The evolution of a warm water mass related to the Iberian Poleward Current (IPC) was characterized along the northern Galician shelf in November 2008 by means of Sea Surface Temperature and wind ...data. It was observed that under upwelling favorable conditions water temperature decreased along the northern coast and a temperature break appeared between Cape Vilano and Cape Ortegal showing a relaxation of the poleward intrusion. The effect of the IPC was also analyzed inside the Northern Galician Rias taking into account the hydrographical and biogeochemical properties measured on November 18. Water driven by the IPC was observed close to the mouth of the rias, around Cape Estaca de Bares, causing a nutrient salts decrease. Inside the rias a slight biological activity was found near surface resulting from fluvial contributions.
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure-function studies of the ...secretory pathway. Here, we have used P. pastoris to test various models for Golgi trafficking. The experimental approach was to analyze P. pastoris tER-Golgi units by using cryofixed and freeze-substituted cells for electron microscope tomography, immunoelectron microscopy, and serial thin section analysis of entire cells. We find that tER sites and the adjacent Golgi stacks are enclosed in a ribosome-excluding "matrix." Each stack contains three to four cisternae, which can be classified as cis, medial, trans, or trans-Golgi network (TGN). No membrane continuities between compartments were detected. This work provides three major new insights. First, two types of transport vesicles accumulate at the tER-Golgi interface. Morphological analysis indicates that the center of the tER-Golgi interface contains COPII vesicles, whereas the periphery contains COPI vesicles. Second, fenestrae are absent from cis cisternae, but are present in medial through TGN cisternae. The number and distribution of the fenestrae suggest that they form at the edges of the medial cisternae and then migrate inward. Third, intact TGN cisternae apparently peel off from the Golgi stacks and persist for some time in the cytosol, and these "free-floating" TGN cisternae produce clathrin-coated vesicles. These observations are most readily explained by assuming that Golgi cisternae form at the cis face of the stack, progressively mature, and ultimately dissociate from the trans face of the stack.
In plants, cell-to-cell communication is mediated by plasmodesmata and involves the trafficking of non-cell-autonomous proteins (NCAPs). A component in this pathway, Nicotiana tabacum ...Non-Cell-Autonomous Pathway Protein1 (NtNCAPP1), was affinity purified and cloned. Protein overlay assays and in vivo studies showed that NtNCAPP1 is located on the endoplasmic reticulum at the cell periphery and displays specificity in its interaction with NCAPs. Deletion of the NtNCAPP1 amino-terminal transmembrane domain produced a dominant-negative mutant that blocked the trafficking of specific NCAPs. Transgenic tobacco plants expressing this mutant form of NtNCAPP1 and plants in which the NtNCAPP1 gene was silenced were compromised in their ability to regulate leaf and floral development. These results support a model in which NCAP delivery to plasmodesmata is both selective and regulated.
The signals regulating stem cell activation during tissue regeneration remain poorly understood. We investigated the baldness associated with mutations in the voltage-gated calcium channel (VGCC) Ca ...sub(v)1.2 underlying Timothy syndrome (TS). While hair follicle stem cells express Ca sub(v)1.2, they lack detectable voltage-dependent calcium currents. Ca sub(v)1.2 super(TS) acts in a dominant-negative manner to markedly delay anagen, while L-type channel blockers act through Ca sub(v)1.2 to induce anagen and overcome the TS phenotype. Ca sub(v)1.2 regulates production of the bulge-derived BMP inhibitor follistatin-likel (Fstl1), derepressing stem cell quiescence. Our findings show how channels act in nonexcitable tissues to regulate stem cells and may lead to novel therapeutics for tissue regeneration.
Abstract
A search for neutrinos produced in coincidence with gamma-ray bursts (GRBs) was conducted with the Super-Kamiokande (SK) detector. Between December 2008 and March 2017, the Gamma-ray ...Coordinates Network recorded 2208 GRBs that occurred during normal SK operation. Several time windows around each GRB were used to search for coincident neutrino events. No statistically significant signal in excess of the estimated backgrounds was detected. The $\bar\nu_e$ fluence in the range from 8 MeV to 100 MeV in positron total energy for $\bar\nu_e+p\rightarrow e^{+}+n$ was found to be less than $\rm 5.07\times10^5$ cm$^{-2}$ per GRB at a 90% confidence level. For all GRBs, upper bounds were obtained on the fluence as a function of neutrino energy. Additionally, for GRBs at known distances, upper limits were set for the neutrino energy emission at the GRB.
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