The functional properties of Caryodendron orinocense protein product were investigated and compared with those of soybean (Glycina maxima). The product protein content was 24.47 g/100 g (Nx6.25). ...Solubility increased at both sides of the isoelectric point (pH 4.0) and with increased NaCl concentration up to 0.5M. Compared with soybean flour (50% protein), the protein product exhibited higher water and oil absorption, but lower emulsifying activity, emulsion stability, foaming capacity, and foam stability, the last one increase at higher pH. Emulsifying activity, foaming capacity, and foam stability were ionic strength dependent. C. orinocense protein product increased its emulsifying activity steadily from 0.05M to 0.75M NaCl, while it remained almost constant for soybean flour. Foaming capacity increased drastically at pH 10. The minimum time and concentration to form a gel was 20% in 4 min and 10% in 8 min for the Caryodendron protein product and soybean flour, respectively. The bulk density was 0.5056+/-0.0041 g/mL.
Glutaredoxin catalyzes glutathione-dependent disulfide oxidoreduction reactions in a coupled system with NADPH, GSH and glutathione reductase and has an active site disulfide/dithiol with the ...sequence -Cys-Pro-Tyr-Cys-. Calf thymus glutaredoxin (thioltransferase), which contains two additional structural half-cystine residues, was purified to homogeneity, using a modification of the previously described isolation procedure. This method involved a pI-shift of glutaredoxin, obtained after oxidation of the fully reduced form with hydroxyethyl-disulfide, followed by CM-Sepharose chromatography. On both SDS- and IEF-gels the protein migrated as one band (M(r) 12,000). The pure protein was used to affinity-purify rabbit antiglutaredoxin antibodies obtained by immunization with the oxidized form of glutaredoxin. Using these antibodies the distribution of glutaredoxin was mapped in calf organs and tissues by Western blots and by immunohistochemistry. Glutaredoxin was demonstrated in all organs investigated. Western blots showed the presence of weak additional high molecular weight bands of unknown identity in certain organs. The immunohistochemical analyses revealed that glutaredoxin is highly expressed in a wide variety of cell types, both epithelial and mesenchymal. The distribution and occurrence in the calf organs was similar to that previously described for thioredoxin in the rat. There were some exceptions: e.g., follicular cells in the ovary did not contain immunohistochemically demonstrable glutaredoxin but expressed thioredoxin. Particularly striking were observations of strong glutaredoxin immunoreactivity in oocytes in the ovary and the pattern of glutaredoxin in epithelial tissue of the skin and tongue reflecting differential expression during cell differentiation. The distribution demonstrated that glutaredoxin serves functions apart from the originally described role as hydrogen donor for ribonucleotide reductase which only occurs in replicating cells. Such functions should relate particularly to glutathione-catalyzed protein disulfide oxidoreductions and cellular signalling by redox regulating mechanisms.
Architecture of the ATLAS High Level Trigger Event Selection Software Armstrong, S.; Assamagan, K.; Baines, J.T. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
02/2004, Letnik:
518, Številka:
1
Journal Article
Recenzirano
Odprti dostop
We present an overview of the strategy for Event Selection at the ATLAS High Level Trigger and describe the architecture and main components of the software developed for this purpose.
Padilla asks what vision should determine the mission of the church of Jesus Christ in the midst of the dramatic socioeconomic, political, cultural, and religious changes taking place in Latin ...America and what should be the role of the church as it looks toward the future of a world in profound transition.
The OTELO survey Cedrés, B; Bongiovanni, Á; Cerviño, M ...
Astronomy and astrophysics (Berlin),
05/2021, Letnik:
649
Journal Article
Recenzirano
Odprti dostop
Aims. In this paper, we aim to study the main properties and luminosity function (LF) of the O II3727 emitters detected in the OTELO survey in order to characterise the star formation processes in ...low-mass galaxies at z ∼ 1.43 and to constrain the faint-end of the LF. Methods. Here, we describe the selection method and analysis of the emitters obtained from narrow-band scanning techniques. In addition, we present several relevant properties of the emitters and discuss the selection biases and uncertainties in the determination of the LF and the star formation rate density (SFRD). Results. We confirmed a total of 60 sources from a preliminary list of 332 candidates as O II3727 emitters. Approximately 93% of the emitters have masses in the range of 108 < M*/M⊙ < 109. All of our emitters are classified as late-type galaxies, with a lower value of (u − v) when compared with the rest of the emitters of the OTELO survey. We find that the cosmic variance strongly affects the normalisation (ϕ*) of the LF and explains the discrepancy of our results when compared with those obtained from surveys of much larger volumes. However, we are able to determine the faint-end slope of the LF, namely, α = −1.42 ± 0.06, by sampling the LF down to ∼1 dex lower than in previous works. We present our calculation of the SFRD of our sample and compare it to the value obtained in previous studies from the literature.
This work presents the results of using a new tool to simulate the cogeneration of water and electricity with Concentrating Solar Power (CSP) and Forward Feed Multi-Effect-Desalination (FF-MED) ...plants, by adding a new functionality to the System Advisor Model (SAM) developed by the US National Renewable Energy Laboratory (NREL). The controlling strategy of the MED model is presented in detail, and a case study application is shown. This study compares the results obtained with a CSP plant operating in San Diego, CA, with four different cooling systems: an MED/Seawater Cooling Circuit (SWCC), dry cooling, wet cooling, and a SWCC standalone. The results show that the usage of an MED/SWCC system in cogeneration with a CSP plant can be feasible and has the potential to be economically interesting.
Acidic ribosomal P1 and P2b proteins, referred to as P proteins, and histone H3 are reported for first time in the
Leishmania braziliensis complex. Deoxyribonucleic acid analysis and multiple ...sequence alignment suggest that both P proteins may maintain their structural function in the ribosomal stalk, in spite of the high rate of mutations detected. The deduced amino acid sequence of protein P1 showed 51% identity with
Trypanosoma cruzi protein P1 and protein P2b showed 61% identity with
T. cruzi protein P2b. Another conserved protein,
L. (Viannia) braziliensis histone H3, showed 82% and 70% identity with histone H3 of
L. (Leishmania) infantum and
T. cruzi, respectively. The N-terminal end of this histone is divergent in comparison with the consensus eukaryotic sequence. Their predicted tridimensional structure was designed.
The results of a search for new heavy
W
′
bosons decaying to an electron or muon and a neutrino using proton–proton collision data at a centre-of-mass energy of
s
=
13
TeV are presented. The dataset ...was collected in 2015 and 2016 by the ATLAS experiment at the Large Hadron Collider and corresponds to an integrated luminosity of 36.1
fb
-
1
. As no excess of events above the Standard Model prediction is observed, the results are used to set upper limits on the
W
′
boson cross-section times branching ratio to an electron or muon and a neutrino as a function of the
W
′
mass. Assuming a
W
′
boson with the same couplings as the Standard Model
W
boson,
W
′
masses below 5.1 TeV are excluded at the 95% confidence level.
Genotypic characteristics of human immunodeficiency virus type 1 (HIV-1) in Mexico were investigated in a multicenter study that involved centers in five geographic regions of the country. Study ...samples (n = 65) collected from male patients in 1998-1999 were sequenced within the C2-V5 region of the gp120 env gene. Phylogenetic analysis revealed that subtype B predominates in Mexico. The level of interpatient nucleotide diversity (mean value of 8.9%) was congruent with multiple introductions of the virus and the "aging" epidemic in Mexico. One-third of samples (30.8% of cases) showed polymorphism within the crown of the V3 loop demonstrating non-GPGR motifs. Two new motifs in the V3 loop crown - HPGG and GPEG - were observed. The evolution of the AIDS epidemic in Mexico should be closely monitored since non-B HIV-1 subtypes might be introduced. The nucleotide sequences were deposited in the GenBank under accession numbers AF200855-AF200869, AF200871-AF200892, and AF200894-AF200921.
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