Background and Purpose
Bradykinin (BK‐(1–9)) is an endogenous nonapeptide involved in multiple physiological and pathological processes. Peptide fragments of bradykinin are believed to be ...biologically inactive. We have now tested the two major peptide fragments of bradykinin in human and animals.
Experimental Approach
BK peptides were quantified by MS in male rats. NO release was quantified from human, mouse and rat cells loaded with DAF‐FM. Rat aortic rings were used to measure vascular reactivity. Changes in BP and HR were measured in conscious male rats. To evaluate pro‐inflammatory effects both vascular permeability and nociception were measured in adult mice.
Key Results
BK‐(1–7) and BK‐(1–5) are produced in vivo from BK‐(1–9). Both peptides induced NO production in all cell types tested. However, unlike BK‐(1–9), NO production elicited by BK‐(1–7) or BK‐(1–5) was not inhibited by B1 or B2 receptor antagonists. BK‐(1–7) and BK‐(1–5) induced concentration‐dependent vasorelaxation of aortic rings, without involvement of B1 or B2 receptors. Intravenous or intra‐arterial administration of BK‐(1–7) or BK‐(1–5) induced similar hypotensive response in vivo. Nociceptive responses of BK‐(1–7) and BK‐(1–5) were reduced compared to BK‐(1–9), and no increase in vascular permeability was observed for BK‐(1–9) fragments.
Conclusions and Implications
BK‐(1–7) and BK‐(1–5) are endogenous peptides present in plasma. BK‐related peptide fragments show biological activity, not mediated by B1 or B2 receptors. These BK fragments could constitute new, active components of the kallikrein–kinin system.
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•HYP/P123 polymeric micelles for photoinactivation were formulated.•Association of HYP/P123 and low-intensity orange LED was effective in photoinactivation.•Mixture design determined ...Staphylococcus aureus reduction times.•HYP/P123 formulations did not photoinhibit biofilms.
Photoinactivation is a promising technique for Staphylococcus aureus control. This microorganism causes foodborne diseases (DTAs) and forms biofilms that are highly resistant and difficult to eradicate. Thus, the aim of this study was to evaluate the photodynamic activity of hypericin (HYP) in polymeric nanoparticles (Pluronic® P123) against S. aureus planktonic and biofilm cells. Planktonic cells and biofilms of S. aureus (ATCC 25923) were subjected to photoinactivation using low-power orange LED (0.3 mW/cm²) with different HYP formulation concentrations in Pluronic® P123. The P123 molar ratios were 2.5 (HYP/P123-2.5) and 10 (HYP/P123-10), respectively. The treatment times for planktonic cells were proposed by a mixture design, and bacterial photoinactivation was observed in concentrations of 12.5 to 3.12 μmol/L for HYP/P123-2.5 and reductions of ∼ 4.0 log CFU/mL in 12.5 to 0.78 μmol/L for HYP/P123-10. For biofilms, 30 min of darkness and 30 min of illumination were used. Maximum reductions were similar for both formulations and corresponded to approximately 0.9 log CFU/cm². It was concluded that photoinactivation with longer lighting times was effective against planktonic cells and could be potentially applied to control S. aureus.
O sorgo (Sorghum L. moench) é uma poaceae com características morfofisiológicas que conferem adaptaçãoàs condições de deficiência hídrica, o que a torna uma alternativa promissora para o Alto Sertão ...Sergipano.Uma maneira de selecionar materiais promissores de sorgo no melhoramento é analisar as correlações entreas variáveis morfológicas. Assim, objetivou-se utilizar a análise de trilha para seleção indireta de sorgoforrageiro com base nas características morfológicas na região do Alto Sertão Sergipano. O experimento foiconduzido na fazenda experimental da Embrapa Semiárido, em Nossa Senhora da Glória (SE), comdelineamento experimental em blocos casualizados e três repetições. Foram utilizados 16 tratamentos, sendo quatro testemunhas comerciais e 12 híbridos experimentais, total de 48 parcelas. As variáveis estudadas foram altura de planta, diâmetro do colmo, comprimento da panícula, estande final, peso do colmo, peso da folha, peso da panícula, peso da planta, matéria fresca, matéria seca, porcentagem de matéria seca e massa de forragem. Houve variabilidade entre os genótipos, e correlações fenotípicas fortes e positivas do peso da planta com peso do colmo, diâmetro do colmo e massa de forragem de 0,99, 0,92 e 0,78, respectivamente. A análise de trilha mostrou que o peso da planta apresentou maior correlação positiva (0,798) e alto efeito direto sobre a massa de forragem (0,949). Assim, recomenda-se a seleção indireta de sorgo com base na característica fenotípica maior massa de forragem, por meio do maior peso de planta.
Abstract The spermatic membrane is rich in polyunsaturated fatty acids, which makes it sensitive to the action of reative species of oxygen, which can damage the seminal quality of the scraps. The ...purpose of this study was to evaluate the effect of the supplementation of two selenium sources at different doses. Third five scraps were allocated in four groups: (INOR30) 0.30 ppm sodium selenite; (COMP30) 0.30 ppm selenium metal-amino acid; (MIXED15+15) 0.15 ppm sodium selenite + 0.15 ppm selenium metal-amino acid and (COMP15) 0.15 ppm selenium metal-amino acid. The ejaculates of the scraps were evaluated over 22 weeks, resulting in 210 samples evaluated for volume, motility, pH, presence of agglutination and morphological changes, and 140 samples for spermatic concentration. The data was analyzed with repeated measures in time in a mixed model with type of selenium supplementation, periods of evaluation (one period of two weeks + five periods of four weeks) and their interactions as fixed effects, and animal and the worker that collected the ejaculates as random effects. Results showed no difference in selenium supplementation with the sources and doses used. In this way, it was possible to verify that the metal amino acid of selenium at the dose of 0.15 ppm promotes the same effect as the diets formulated with 0.30 ppm of sodium selenite.
Resumo A membrana espermática é rica em ácidos graxos poliinsaturados, o que a torna sensível à ação de espécies reativas de oxigênio, que podem prejudicar a qualidade seminal dos cachaços. O objetivo do presente estudo foi avaliar o efeito da suplementação de duas fontes de selênio em diferentes doses. Trinta e cinco cachaços foram distribuídos em quatro grupos: (INOR30) 0,30 ppm de selenito de sódio; (COMP30) 0,30 ppm de metal-aminoácido de selênio; (MISTO15+15) 0,15 ppm de selenito de sódio + 0,15 ppm de metal-aminoácido de selênio e (COMP15) 0,15 ppm de metal-aminoácido de selênio. Os ejaculados dos cachaços foram avaliados durante 22 semanas, resultando em 210 amostras avaliadas para volume, motilidade, pH, presença de aglutinação e alterações morfológicas, e 140 amostras para concentração espermática. Os dados foram analisados com medidas repetidas no tempo em modelo misto, em que o tipo de suplementação de selênio, os períodos de avaliação (um período de duas semanas + cinco períodos de quatro semanas) e suas interações foram os efeitos fixos, e o animal e o funcionário que coletou os ejaculados foram os efeitos aleatórios. Os resultados obtidos demonstraram não haver diferença na suplementação de selênio com as fontes e doses utilizadas. Com isso, foi possível verificar que o metal-aminoácido de selênio na dose de 0,15 ppm promove o mesmo efeito das dietas formuladas com 0,30 ppm de selenito de sódio.
Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate ...protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.
This work shows a research about the conceptions of Environmental Education of newly formed sciences teachers from the course of Degree in Sciences of the Federal University of Paraná, Litoral ...Sector. The research was qualitative with interviews by guidelines with eight of twenty of the recent graduates. The interviews were transcribed and the method to analyze was Content Analysis. The main units of analysis that emerged were: integrated understanding of society and the environment; work as an activity of interest and employment with a focus on income; understanding of interdisciplinarity in the context of environmental issues and; Environmental Education with critical and emancipatory aspects. The outcomes show an understanding of work related to the dominant ideology through market principles. An integrated understanding of society and environment that involves environmental and social aspects. These understandings are interlaced with the understandings related to interdisciplinarity and Environmental Education involving critical and emancipatory aspects.
Bradykinin (BK-(1-9)) is an endogenous nonapeptide involved in multiple physiological and pathological processes. Peptide fragments of bradykinin are believed to be biologically inactive. We have now ...tested the two major peptide fragments of bradykinin in human and animals.
BK peptides were quantified by MS in male rats. NO release was quantified from human, mouse and rat cells loaded with DAF-FM. Rat aortic rings were used to measure vascular reactivity. Changes in BP and HR were measured in conscious male rats. To evaluate pro-inflammatory effects both vascular permeability and nociception were measured in adult mice.
BK-(1-7) and BK-(1-5) are produced in vivo from BK-(1-9). Both peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B
or B
receptor antagonists. BK-(1-7) and BK-(1-5) induced concentration-dependent vasorelaxation of aortic rings, without involvement of B
or B
receptors. Intravenous or intra-arterial administration of BK-(1-7) or BK-(1-5) induced similar hypotensive response in vivo. Nociceptive responses of BK-(1-7) and BK-(1-5) were reduced compared to BK-(1-9), and no increase in vascular permeability was observed for BK-(1-9) fragments.
BK-(1-7) and BK-(1-5) are endogenous peptides present in plasma. BK-related peptide fragments show biological activity, not mediated by B
or B
receptors. These BK fragments could constitute new, active components of the kallikrein-kinin system.
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