Bioelectronic interfaces require electrodes that are mechanically flexible and chemically inert. Flexibility allows pristine electrode contact to skin and tissue, and chemical inertness prevents ...electrodes from reacting with biological fluids and living tissues. Therefore, flexible gold electrodes are ideal for bioimpedance and biopotential measurements such as bioimpedance tomography, electrocardiography (ECG), electroencephalography (EEG), and electromyography (EMG). However, a manufacturing process to fabricate gold electrode arrays on plastic substrates is still elusive. In this work, a fabrication and low‐temperature sintering (≈200 °C) technique is demonstrated to fabricate gold electrodes. At low‐temperature sintering conditions, lines of different widths demonstrate different sintering speeds. Therefore, the sintering condition is targeted toward the widest feature in the design layout. Manufactured electrodes show minimum feature size of 62 μm and conductivity values of 5 × 10 6 S m−1. Utilizing the versatility of printing and plastic electronic processes, electrode arrays consisting of 31 electrodes with electrode‐to‐electrode spacing ranging from 2 to 7 mm are fabricated and used for impedance mapping of conformal surfaces at 15 kHz. Overall, the fabrication process of an inkjet‐printed gold electrode array that is electrically reproducible, mechanically robust, and promising for bioimpedance and biopotential measurements is demonstrated.
Fabrication of inkjet‐printed flexible gold electrode arrays on plastic substrates is described, with a special focus on laser‐cut freestanding electrodes, low‐temperature sintering, and the methodology used for impedance mapping on conformal surfaces. Taking advantage of low‐cost and large‐area manufacturing techniques, these electrically reproducible and mechanically robust electrode arrays are promising for novel wearable biomedical sensing.
Chitosan in Nanostructured Thin Films Pavinatto, Felippe J; Caseli, Luciano; Oliveira, Osvaldo N
Biomacromolecules,
08/2010, Letnik:
11, Številka:
8
Journal Article
Recenzirano
This review paper brings an overview of the use of chitosans in nanostructured films produced with the Langmuir−Blodgett (LB) or the electrostatic layer-by-layer (LbL) techniques, with emphasis on ...their possible applications. From a survey in the literature one may identify three main types of study with chitosan in nanostructured films. First, the interaction between chitosans and phospholipid Langmuir monolayers has been investigated for probing the mechanisms of chitosan action in their biological applications, with the monolayers serving as cell membrane models. In the second type, chitosan serves as a matrix for immobilization of biomolecules in LB as well as in LbL films, for which chitosan is suitable to help preserve the bioactivity of such biomolecules for long periods of time even in dry, solid films. An important application of these chitosan-containing films is in sensing and biosensing. The third type of study involves exploiting the mechanical and biocompatibility properties of chitosan in producing films with enhanced properties, for example, for tissue engineering. It is emphasized that chitosans have been proven excellent building blocks to produce films with controlled molecular architecture, allowing for synergy between distinct materials. We also discuss the prospects of the field, following a critical review of the latest developments in nanostructured chitosan films.
Printing techniques have been extensively used in the fabrication of organic electronic devices, such as light-emitting diodes and display backplanes. These techniques, in particular inkjet printing, ...are being employed for the localized dispensing of solutions containing biological molecules and cells, leading to the fabrication of bio-functional microarrays and biosensors. Here, we report the fabrication of an all-printed and flexible biosensor for antioxidants. Gold (Au) interdigitated electrodes (IDEs) with sub-100 µm features were directly inkjet-printed on plastic substrates using a nanoparticle-based ink. Conductivities as high as 5×10(6) S/m (12% of bulk Au) were attained after sintering was conducted at plastic-compatible 200 °C for 6 h. The enzyme Tyrosinase (Tyr) was used in the active layer of the biosensors, being innovatively deposited by large-area rotogravure printing. A tailor-made ink was studied, and the residual activity of the enzyme was 85% after additives incorporation, and 15.5% after gravure printing. Au IDEs were coated with gravure films of the Tyr-containing ink, and the biosensor was encapsulated with a cellulose acetate dip-coating film to avoid dissolution. The biosensor impedance magnitude increases linearly with the concentration of a model antioxidant, allowing for the construction of a calibration curve. Control experiments demonstrated the molecular recognition characteristic inferred by the enzyme. We found that the biosensor sensitivity and the limit of detection were, respectively, 5.68 Ω/µm and 200 µM. In conclusion, a disposable, light-weight, all-printed and flexible biosensor for antioxidants was successfully fabricated using fast and large-area printing techniques. This opens the door for the fabrication of technological products using roll-to-roll processes.
When pressure is applied to a localized area of the body for an extended time, the resulting loss of blood flow and subsequent reperfusion to the tissue causes cell death and a pressure ulcer ...develops. Preventing pressure ulcers is challenging because the combination of pressure and time that results in tissue damage varies widely between patients, and the underlying damage is often severe by the time a surface wound becomes visible. Currently, no method exists to detect early tissue damage and enable intervention. Here we demonstrate a flexible, electronic device that non-invasively maps pressure-induced tissue damage, even when such damage cannot be visually observed. Using impedance spectroscopy across flexible electrode arrays in vivo on a rat model, we find that impedance is robustly correlated with tissue health across multiple animals and wound types. Our results demonstrate the feasibility of an automated, non-invasive 'smart bandage' for early detection of pressure ulcers.
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► Electrostatic interactions govern interaction between mucin and chitosan in a cell membrane model. ► Mucin expands Langmuir monolayers from negatively charged phospholipid. ► ...Chitosan is able to remove mucin from a phospholipid monolayer. ► Desorption of mucin from LB films was induced by chitosan.
Chitosans have been widely exploited in biological applications, including drug delivery and tissue engineering, especially owing to their mucoadhesive properties, but the molecular-level mechanisms for the chitosan action are not known in detail. It is believed that chitosan could affect the mucus by interacting with the proteins mucins, in a process mediated by the cell membrane. In this study we used Langmuir monolayers of dimyristoylphosphatidic acid (DMPA) as simplified membrane models to investigate the interplay between the activity of mucins and chitosan. Surface pressure and surface potential measurements were performed with DMPA monolayers onto which chitosan and/or mucin was adsorbed. We found that the expanding effect from mucin was considerably reduced when chitosan was injected after mucin had been adsorbed on the DMPA monolayer. The results were consistent with the formation of complexes between mucin and chitosan, thus highlighting the importance of electrostatic interactions. Furthermore, chitosan could remove mucin that was co-deposited along with DMPA in Langmuir–Blodgett (LB) films, which could be ascribed to molecular-level interactions between chitosan and mucin inferred from the FTIR spectra of the LB films. In conclusion, the results with Langmuir and LB films suggest that electrostatic interactions are crucial for the mucoadhesive mechanism, which is affected by the complexation between chitosan and mucin.
Incorporation into cell membranes is key for the action of photosensitizers in photomedicine treatments, with hydroperoxidation as the prominent pathway of lipid oxidation. In this paper, we use ...Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as cell membrane models to investigate adsorption of the photosensitizer erythrosin and its effect on photoinduced lipid oxidation. From surface pressure isotherms and polarization-modulated infrared reflection–absorption spectroscopy (PM-IRRAS) data, erythrosin was found to adsorb mainly via electrostatic interaction with the choline in the head groups of both DOPC and DPPC. It caused larger monolayer expansion in DOPC, with possible penetration into the hydrophobic unsaturated chains, while penetration into the DPPC saturated chains was insignificant. Easier penetration is due to the less packed DOPC monolayer, in comparison to the more compact DPPC according to the monolayer compressibility data. Most importantly, light irradiation at 530 nm made the erythrosin-containing DOPC monolayer become less unstable, with a relative surface area increase of ca. 19%, in agreement with previous findings for bioadhesive giant vesicles. The relative area increase is consistent with hydroperoxidation, supporting the erythrosin penetration into the lipid chains, which favors singlet oxygen generation close to double bonds, an important requirement for photodynamic efficiency.
A lead-zirconate-titanate (PZT) thin film additively manufactured on a flexible substrate is demonstrated in this article as an actuator. PZT nano-particles (NPs) are first fabricated via a ...hydrothermal process. The NPs are then suspended in ethanol with a polymer additive to form PZT ink. The PZT ink is later drop-cast on a flexible substrate with two electrode designs for evaluation: sandwich electrodes and interdigitated electrodes (IDEs). The design of sandwich electrodes is difficult to realize because silver electrodes cannot be properly printed onto the PZT film with good conductivity and dimension accuracy. In contrast, a pair of IDEs is successfully inkjet printed on polyethylene terephthalate (PET) tape. Then the PZT ink is drop-cast to form a PZT film. Sinusoidal voltage applied over the electrodes drives the device into resonance serving as a resonator. For hard-disk drives, the 3-D printed PZT films can be used as dual-stage actuators or as absorbers to actively or passively reduce flex cable vibration.
The interaction between chitosan and Langmuir and Langmuir−Blodgett (LB) films of dimyristoyl phosphatidic acid (DMPA) is investigated, with the films serving as simplified cell membrane models. At ...the air−water interface, chitosan modulates the structural properties of DMPA monolayers, causing expansion and decreasing the monolayer elasticity. As the surface pressure increased, some chitosan molecules remained at the interface, but others were expelled. Chitosan could be transferred onto solid supports alongside DMPA using the LB technique, as confirmed by infrared spectroscopy and quartz crystal microbalance measurements. The analysis of sum-frequency vibration spectroscopy data for the LB films combined with surface potential measurements for the monolayers pointed to chitosan inducing the ordering of the DMPA alkyl chains. Furthermore, the morphology of DMPA LB films, studied with atomic force microscopy, was affected significantly by the incorporation of chitosan, with the mixed chitosan−DMPA films displaying considerably higher thickness and roughness, in addition to chitosan aggregates. Because chitosan affected DMPA films even at pressures characteristic of cell membranes, we believe this study may help elucidate the role of chitosan in biological systems.
•The low oral and topical toxicity of natamycin for mammalian cells was explained.•We investigate the effect of natamycin on Langmuir monolayers (model membranes).•The effects of natamycin depend on ...the cholesterol content in the monolayers.•Significant effects are verified for 50mol% or higher cholesterol content.•The effects are negligible for 25mol% cholesterol content (mammalian membranes).
Natamycin is an effective, broad spectrum antifungal with no reported resistance, in contrast to most antimicrobials. It also exhibits reduced (oral and topical) toxicity to humans, which is probably associated with the lack of effects on mammalian cell membranes. In this paper we employ Langmuir monolayers to mimic a cell membrane, whose properties are interrogated with various techniques. We found that natamycin has negligible effects on Langmuir monolayers of dipalmitoyl phosphatidylcholine (DPPC), but it strongly affects cholesterol monolayers. Natamycin causes the surface pressure isotherm of a cholesterol monolayer to expand even at high surface pressures since it penetrates into the hydrophobic chains. It also reduces the compressibility modulus, probably because natamycin disturbs the organization of the cholesterol molecules, as inferred with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In mixed cholesterol/DPPC monolayers, strong effects from natamycin were only observed when the cholesterol concentration was 50mol% or higher, well above its concentration in a mammalian cell membrane. For a sterol concentration that mimics a real cell membrane in mammals, i.e. with 25mol% of cholesterol, the effects were negligible, which may explain why natamycin has low toxicity when ingested and/or employed to treat superficial fungal infections.
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