Test beam measurements at the test beam facilities of DESY have been conducted to characterise the performance of the EUDET-type beam telescopes originally developed within the EUDET project. The ...beam telescopes are equipped with six sensor planes using MIMOSA 26 monolithic active pixel devices. A programmable Trigger Logic Unit provides trigger logic and time stamp information on particle passage. Both data acquisition framework and offline reconstruction software packages are available. User devices are easily integrable into the data acquisition framework via predefined interfaces.
The biased residual distribution is studied as a function of the beam energy, plane spacing and sensor threshold. Its standard deviation at the two centre pixel planes using all six planes for tracking in a 6 GeV electron/positron-beam is measured to be (2.88 ± 0.08) µm. Iterative track fits using the formalism of General Broken Lines are performed to estimate the intrinsic resolution of the individual pixel planes. The mean intrinsic resolution over the six sensors used is found to be (3.24 ± 0.09) µm. With a 5 GeV electron/positron beam, the track resolution halfway between the two inner pixel planes using an equidistant plane spacing of 20 mm is estimated to (1.83 ± 0.03) µm assuming the measured intrinsic resolution. Towards lower beam energies the track resolution deteriorates due to increasing multiple scattering. Threshold studies show an optimal working point of the MIMOSA 26 sensors at a sensor threshold of between five and six times their RMS noise. Measurements at different plane spacings are used to calibrate the amount of multiple scattering in the material traversed and allow for corrections to the predicted angular scattering for electron beams.
The fast and safe removal of ticks is of medical and veterinary importance since many tick-borne pathogens require time to be transmitted. In the past, many tools and applications were used to remove ...ticks from the skin of humans and pets. Choking the ticks by blocking their respiratory system with chemicals cannot be recommended due to the low respiratory rate of ticks. Mechanical devices to remove ticks are usually recommended; however, they vary with regard to their mechanism of seizing and holding the tick and in the way of extraction (pulling or twisting). In this study, five commercial tick removal devices with different mechanisms were tested on pets according to their practicability, injury of the mouthparts, and the idiosoma of female
Ixodes ricinus
ticks. Therefore, 22 veterinarians and four pet owners removed 596 ticks from various animals by using the different devices and filled in a questionnaire for each case. The tick species and instars were determined, and for the female
I. ricinus
ticks (
n
= 527) the condition of the mouthparts as well as the idiosoma was evaluated. Twisting of the female
I. ricinus
ticks reduced the force required for extraction, the adverse reaction of the animal and the time needed for removal. The device with a “V”-shaped slot which allows a grabbing of the mouthparts delivered the best results according to the condition of the mouthparts and the intactness of the female
I. ricinus
tick’s body. Therefore, grabbing the mouthparts and twisting can be recommended for removal of
I. ricinus
females from pets.
The new silicon microstrip sensors of the End-cap part of the HL-LHC ATLAS Inner Tracker (ITk) present a number of challenges due to their complex design features such as the multiple different ...sensor shapes, the varying strip pitch, or the built-in stereo angle. In order to investigate these specific problems, the “petalet” prototype was defined as a small End-cap prototype. The sensors for the petalet prototype include several new layout and technological solutions to investigate the issues, they have been tested in detail by the collaboration. The sensor description and detailed test results are presented in this paper. New software tools have been developed for the automatic layout generation of the complex designs. The sensors have been fabricated, characterized and delivered to the institutes in the collaboration for their assembly on petalet prototypes. This paper describes the lessons learnt from the design and tests of the new solutions implemented on these sensors, which are being used for the full petal sensor development. This has resulted in the ITk strip community acquiring the necessary expertise to develop the full End-cap structure, the petal.
Infection with the ubiquitous parasite Toxoplasma gondii is a threat for immunocompromised patients and pregnant women and effective immune-prophylaxis is still lacking.
Here we tested a mixture of ...recombinant T. gondii antigens expressed in different developmental stages, i.e., SAG1, MAG1 and GRA7 (SMG), and a lysate derived from T. gondii tachyzoites (TLA) for prophylactic vaccination against cyst formation. Both vaccine formulations were applied systemically followed by an oral TLA-booster in BALB/c mice.
Systemic priming with SMG and oral TLA-booster did not show significant induction of protective immune responses. In contrast, systemic priming and oral booster with TLA induced higher levels of Toxoplasma-specific IgG, IgG1 and IgG2a in sera as well as high levels of Toxoplasma-specific IgG1 in small intestines. Furthermore, high levels of Toxoplasma-specific Th1-, Th17- and Th2-associated cytokines were only detected in restimulated splenocytes of TLA-vaccinated mice. Importantly, in mice orally infected with T. gondii oocysts, only TLA-vaccination and booster reduced brain cysts. Furthermore, sera from these mice reduced tachyzoites invasion of Vero cells in vitro, indicating that antibodies may play a critical role for protection against Toxoplasma infection. Additionally, supernatants from splenocyte cultures of TLA-vaccinated mice containing high levels of IFN-γ lead to substantial production of nitric oxide (NO) after incubation with macrophages in vitro. Since NO is involved in the control of parasite growth, the high levels of IFN-γ induced by vaccination with TLA may contribute to the protection against T. gondii.
In conclusion, our data indicate that prime-boost approach with TLA, but not with the mixture of recombinant antigens SMG, induces effective humoral and cellular Toxoplasma-specific responses and leads to significant reduction of cerebral cysts, thereby presenting a viable strategy for further vaccine development against T. gondii infection.
Two trials were carried out to compare the use of different enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against Sarcoptes scabiei var. suis. In the first trial, we ...employed four tests and 70 selected sera from a closed pig farm with sarcoptic mange. The sera were taken from the breeding area as well as from the finishing unit and compared with skin scrapings. The SARCOPTES-ELISA 2001 yielded the most positive results (88.58%), followed by the ELISA of the National Veterinary Institute, Uppsala (70%), the Acar-Test P-ELISA (52.86%), skin scrapings (48.57%), and the CHEKIT Sarcoptest (30%). In the second trial, eight litters from infected sows were examined from weeks 1–12 of life using the CHEKIT Sarcoptest and the SARCOPTES-ELISA 2001. The presence of maternal antibodies was highest on day 7 in both ELISAs and could be detected until weeks 5–9 of life. Antibodies increased as a result of an active immune response between days 56 and 63 in the SARCOPTES-ELISA 2001 and between days 70 and 77 of life in the CHEKIT Sarcoptest. Significant differences between the first and second litters were observed.
The planned High Luminosity Large Hadron Collider is being designed to maximise the physics potential of the LHC with 10 years of operation at instantaneous luminosities of 7.5×1034cm−2s−1. A ...consequence of this increased luminosity is the expected radiation damage requiring the tracking detectors to withstand hadron fluence to over 1×1015 1 MeV neutron equivalent per cm2 in the ATLAS Strips system. Fast readout electronics, deploying 130 nm CMOS front-end electronics are glued on top of a silicon sensor to make a module. The radiation hard n-in-p micro-strip sensors used have been developed by the ATLAS ITk Strip Sensor collaboration and produced by Hamamatsu Photonics. A series of tests were performed at the DESY-II test beam facility to investigate the detailed performance of a strip module with both 2.5 cm and 5 cm length strips before irradiation. The DURANTA telescope was used to obtain a pointing resolution of 2 μm, with an additional pixel layer installed to improve timing resolution to ∼25 ns. Results show that prior to irradiation a wide range of thresholds (0.5–2.0 fC) meet the requirements of a noise occupancy less than 1×10−3 and a hit efficiency greater than 99%.
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