The InSight (Interior Exploration using Seismic Investigations, Geodesy and Heat Transport) mission landed on the surface of Mars on November 26, 2018. One of the scientific instruments in the ...payload that is essential to the mission is the SEIS package (Seismic Experiment for Interior Structure) which includes a very broadband and a short period seismometer. More than one year since the landing, SEIS continues to be fully operational and has been collecting an exceptional data set which contains not only the signals of seismic origins, but also noise and artifacts induced by the martian environment, the hardware on the ground that includes the seismic sensors, and the programmed operational activities of the lander. Many of these non-seismic signals will be unfamiliar to the scientific community. In addition, many of these signals have signatures that may resemble seismic events either or both in time and frequency domains. Here, we report our observations of common non-seismic signals as seen during the first 478 sols of the SEIS data, i.e. from landing until the end of March 2020. This manuscript is intended to provide a guide to scientists who use the data recorded on SEIS, detailing the general attributes of the most commonly observed non-seismic features. It will help to clarify the characteristics of the seismic dataset for future research, and to avoid misinterpretations when searching for marsquakes.
•This paper is a summary of the InSight data from Mars, mainly focusing on the seismic data set.•We describe the signals of non-seismic origins that potentially can cause misinterpretations as marsquakes.•We outline the common features in the data such as artifacts and patterns for future reference that may be unfamiliar to the scientific community.
Interior exploration using Seismic Investigations, Geodesy and Heat Transport's (InSight) seismometer package Seismic Experiment for Interior Structure (SEIS) was placed on the surface of Mars at ...about 1.2 m distance from the thermal properties instrument Heat flow and Physical Properties Package (HP3) that includes a self‐hammering probe. Recording the hammering noise with SEIS provided a unique opportunity to estimate the seismic wave velocities of the shallow regolith at the landing site. However, the value of studying the seismic signals of the hammering was only realized after critical hardware decisions were already taken. Furthermore, the design and nominal operation of both SEIS and HP3 are nonideal for such high‐resolution seismic measurements. Therefore, a series of adaptations had to be implemented to operate the self‐hammering probe as a controlled seismic source and SEIS as a high‐frequency seismic receiver including the design of a high‐precision timing and an innovative high‐frequency sampling workflow. By interpreting the first‐arriving seismic waves as a P‐wave and identifying first‐arriving S‐waves by polarization analysis, we determined effective P‐ and S‐wave velocities of vP=119−21+45 ${v}_{P}=11{9}_{-21}^{+45}$ m/s and vS=63−7+11 ${v}_{S}=6{3}_{-7}^{+11}$ m/s, respectively, from around 2,000 hammer stroke recordings. These velocities likely represent bulk estimates for the uppermost several 10s of cm of regolith. An analysis of the P‐wave incidence angles provided an independent vP/vS ratio estimate of 1.84−0.35+0.89 $1.8{4}_{-0.35}^{+0.89}$ that compares well with the traveltime based estimate of 1.86−0.25+0.42 $1.8{6}_{-0.25}^{+0.42}$. The low seismic velocities are consistent with those observed for low‐density unconsolidated sands and are in agreement with estimates obtained by other methods.
Plain Language Summary
In the framework of the NASA Interior exploration using Seismic Investigations, Geodesy and Heat Transport (InSight) mission, two scientific instruments were placed on the surface of Mars: A seismometer to detect signals from marsquakes and other sources generating seismic (elastic) waves and a self‐hammering temperature sensor that was designed to penetrate the Martian subsurface. The hammering of the temperature sensor generated vibrations that were measured by the seismometer and could be used to determine the elastic parameters of the shallow subsurface of Mars. We found low seismic velocities for the shallowest several tens of cm that are typical for low‐density loose sands. This information is important to further study the local geological setting at the InSight landing site and the shallow Martian subsurface in general.
Key Points
Seismic signals from the Heat flow and Physical Properties Package mole provide a unique opportunity to study the shallow regolith
First‐arrival traveltimes and P‐wave incidence angles constrain elastic parameter estimates
Low seismic velocities are consistent with unconsolidated low‐density sand
In December 2018, the NASA InSight lander successfully placed a seismometer on the surface of Mars. Alongside, a hammering device was deployed at the landing site that penetrated into the ground to ...attempt the first measurements of the planetary heat flow of Mars. The hammering of the heat probe generated repeated seismic signals that were registered by the seismometer and can potentially be used to image the shallow subsurface just below the lander. However, the broad frequency content of the seismic signals generated by the hammering extends beyond the Nyquist frequency governed by the seismometer's sampling rate of 100 samples per second. Here, we propose an algorithm to reconstruct the seismic signals beyond the classical sampling limits. We exploit the structure in the data due to thousands of repeated, only gradually varying hammering signals as the heat probe slowly penetrates into the ground. In addition, we make use of the fact that repeated hammering signals are sub‐sampled differently due to the unsynchronized timing between the hammer strikes and the seismometer recordings. This allows us to reconstruct signals beyond the classical Nyquist frequency limit by enforcing a sparsity constraint on the signal in a modified Radon transform domain. In addition, the proposed method reduces uncorrelated noise in the recorded data. Using both synthetic data and actual data recorded on Mars, we show how the proposed algorithm can be used to reconstruct the high‐frequency hammering signal at very high resolution.
Key Points
Hammering of the InSight heat probe generates high‐frequency seismic signals that exceed the Nyquist frequency of the seismometer
We developed a new data acquisition and reconstruction workflow that allows for the recovery of the full‐bandwidth hammering signals
During hammering, we deliberately turned off the seismometer's anti‐aliasing filters and reconstructed the aliased signal using a sparseness‐promoting algorithm
At the ETH Zurich we are developing a modular simulator that provides a realistic simulation of the Front End Electronics (FEE) for LISA Gravitational Reference Sensor (GRS). It is based on the GRS ...FEE-simulator already implemented for LISA Pathfinder. It considers, in particular, the non-linearity and the critical details of hardware, such as the non-linear multiplicative noise caused by voltage reference instability, test mass charging and detailed actuation and sensing algorithms. We present the simulation modules, considering the above-mentioned features. Based on the ETH GRS FEE-simulator for LISA Pathfinder we aim to develop a modular simulator that provides a realistic simulation of GRS FEE for LISA.
On board LISA Pathfinder spacecraft the test mass displacement along the main measurement axis is sensed in two different ways: optically and electrostatically. We have monitored the relative ...calibration between the two measurements during the mission science phase. The trend sensitivity of the relative calibration has been computed for different physical parameters, such as temperature, magnetic field, test mass bias voltage and current.
Accurate determination of intracellular metabolite levels requires well-validated procedures for sampling and sample treatment. Several methods exist for metabolite extraction, but the literature is ...contradictory regarding the adequacy and performance of each technique. Using a strictly quantitative approach, we have re-evaluated five methods (hot water, HW; boiling ethanol, BE; chloroform−methanol, CM; freezing-thawing in methanol, FTM; acidic acetonitrile−methanol, AANM) for the extraction of 44 intracellular metabolites (phosphorylated intermediates, amino acids, organic acids, nucleotides) from S. cerevisiae cells. Two culture modes were investigated (batch and chemostat) to check for growth condition dependency, and three targeted platforms were employed (two LC-MS and one GC/MS) to exclude analytical bias. Additionally, for the determination of metabolite recoveries, we applied a novel approach based on addition of 13C-labeled internal standards at different stages of sample processing. We found that the choice of extraction method can drastically affect measured metabolite levels, to an extent that for some metabolites even the direction of changes between growth conditions can be inverted. The best performances, in terms of efficacy and metabolite recoveries, were achieved with BE and CM, which yielded nearly identical levels for the metabolites analyzed. According to our results, AANM performs poorly in yeast and FTM cannot be considered adequate as an extraction method, as it does not ensure inactivation of enzymatic activity.
The NASA InSight lander successfully placed a seismometer on the surface of Mars. Alongside, a hammering device was deployed that penetrated into the ground to attempt the first measurements of the ...planetary heat flow of Mars. The hammering generated repeated seismic signals that were registered by the seismometer. These signals can potentially be used to image the shallow subsurface just below the lander. However, the frequencies excited by the hammering probe widely exceed the Nyquist frequency dictated by the seis-mometer's sampling rate. Here, we propose an algorithm to reconstruct the seismic signals beyond the classical sampling theorem. We exploit the structure in the data due to thousands of repeated, only gradually varying hammering signals as the heat probe slowly penetrates into the ground. This allows us to reconstruct signals by enforcing a sparsity constraint in a modified Radon transform domain.
Quantitative metabolomics of microbial cultures requires well-designed sampling and quenching procedures. We successfully developed and applied a differential method to obtain a reliable set of ...metabolome data for
Escherichia coli K12 MG1655 grown in steady-state, aerobic, glucose-limited chemostat cultures. From a rigorous analysis of the commonly applied quenching procedure based on cold aqueous methanol, it was concluded that it was not applicable because of release of a major part of the metabolites from the cells. No positive effect of buffering or increasing the ionic strength of the quenching solution was observed. Application of a differential method in principle requires metabolite measurements in total broth and filtrate for each measurement. Different methods for sampling of culture filtrate were examined, and it was found that direct filtration without cooling of the sample was the most appropriate. Analysis of culture filtrates revealed that most of the central metabolites and amino acids were present in significant amounts outside the cells. Because the turnover time of the pools of extracellular metabolites is much larger than that of the intracellular pools, the differential method should also be applicable to short-term pulse response experiments without requiring measurement of metabolites in the supernatant during the dynamic period.
A gas chromatography isotope dilution mass spectrometry (GC–IDMS) method was developed for the quantification of the metabolites of the non-oxidative part of pentose phosphate pathway (PPP). A ...mid-polar GC column (Zebron ZB-AAA, 10
m, film composition 50% phenyl 50% dimethyl polysiloxane) was used for the chromatographic separation of the intermediates. The optimized GC–MS procedure resulted in improved separation performances and higher sensitivities compared to previous methods. Furthermore, the use of
13C-labeled cell extracts as internal standards improved the data quality and eliminated the need to perform a recovery check for each metabolite. The applicability of the new method was demonstrated by analyzing intracellular metabolite levels in samples derived from aerobic glucose-limited chemostat cultures of
Saccharomyces cerevisiae at steady state as well as following a short-term glucose pulse. The major achievements of the proposed quantitative method are the independent quantification of the epimers ribulose-5-phosphate and xylulose-5-posphate and the measurement of compounds present at very low concentrations in biological samples such as erythrose-4-phosphate and glyceraldehyde-3-phosphate.
Accurate determination of intracellular metabolite levels requires reliable, reproducible techniques for sampling and sample treatment. Quenching in 60% (v/v) methanol at -40°C is currently the ...standard method for sub-second arrest of metabolic activity in microbial metabolomics but there have been contradictory reports in the literature on whether leakage of metabolites from the cells occurs. We have re-evaluated this method in S. cerevisiae using a comprehensive, strictly quantitative approach. By determining the levels of a large range of metabolites in different sample fractions and establishing mass balances we could trace their fate during the quenching procedure and confirm that leakage of metabolites from yeast cells does occur during conventional cold methanol quenching, to such an extent that the levels of most metabolites have been previously underestimated by at least twofold. In addition, we found that the extent of leakage depends on the time of exposure, the temperature and the properties of the methanol solutions. Using the mass balance approach we could study the effect of different quenching conditions and demonstrate that leakage can be entirely prevented by quenching in pure methanol at <=-40°C, which we propose as a new improved method. Making use of improved data on intracellular metabolite levels we also re-evaluated the need of sub-second quenching of metabolic activity and of removing the extracellular medium. Our findings have serious implications for quantitative metabolomics-based fields such as non-stationary ¹³C flux analysis, in vivo kinetic modeling and thermodynamic network analysis.