We report sub-arcsec CO observations of the disks around MWC 480, LkCa 15 and DM Tau, and simultaneous measurements of HCO+ J=1-0. We derive the disk properties by fitting a standard disk model, with ...all parameters power laws of the distance to the star. Biases are explained and discussed. We find evidence for vertical temperature gradient in the disks of MWC 480 and DM Tau, as in AB Aur, but not in LkCa 15. The disks temperature increase with stellar effective temperature. Most of the CO gas is at temperatures smaller than 17 K, the condensation temperature on grains. The scale height of the CO distribution appears larger (by 50%) than the hydrostatic scale height. The more UV luminous stars have more CO, but there is no simple dependency of CO abundance and isotopologue ratio with stellar type. The 13CO/HCO+ ratio is around 600. The temperature behaviour is consistent with expectations, but published chemical models have difficulty reproducing the observed CO quantities. Vertical mixing and photo-dissociation at the disk outer edge seem important chemical agents. The CO data suggest a more complex surface density distribution than assumed in models.
We present sub-arcsecond images of AB Auriga obtained with the IRAM Plateau de Bure interferometer in the isotopologues of CO, and in continuum at 3 and 1.3 mm. Instead of being centrally peaked, the ...continuum emission is dominated by a bright, asymmetric (spiral-like) feature at about 140 AU from the central star. The large scale molecular structure suggests the AB Aur disk is inclined between 23 and 43 degrees, but the strong asymmetry of the continuum and molecular emission prevents an accurate determination of the inclination of the inner parts. We find significant non-Keplerian motion, with a best fit exponent for the rotation velocity law of 0.41 +/- 0.01, but no evidence for radial motions. The disk has an inner hole about 70 AU in radius. The disk is warm and shows no evidence of depletion of CO. The dust properties suggest the dust is less evolved than in typical T Tauri disks. Both the spiral-like feature and the departure from purely Keplerian motions indicates the AB Aur disk is not in quasi-equilibrium. Disk self-gravity is insufficient to create the perturbation. This behavior may be related either to an early phase of star formation in which the Keplerian regime is not yet fully established and/or to a disturbance of yet unknown origin. An alternate, but unproven, possibility is that of a low mass companion located about 40 AU from AB Aur.
We performed sub-arcsecond high-sensitivity nterferometric observations of the thermal dust emission at 1.4 mm and 2.8 mm in the disks surrounding LkCa 15 and MWC 480, with the new 750 m baselines of ...the IRAM PdBI array. This provides a linear resolution of about 60 AU at the Taurus distance. We report the existence of a cavity of about 50 AU radius in the inner disk of LkCa 15. Whereas LkCa 15 emission is optically thin, the optically thick core of MWC 480 is resolved at 1.4 mm with a radius of about 35 AU, constraining the dust temperature. In MWC 480, the dust emission is coming from a colder layer than the CO emission, most likely the disk mid-plane. These observations provide direct evidence of an inner cavity around LkCa 15. Such a cavity most probably results from the tidal disturbance created by a low mass companion or large planet at about 30 AU from the star. These results suggest that planetary system formation is already at work in LkCa 15. They also indicate that the classical steady-state viscous disk model is a too simplistic description of the inner 50 AU of ''proto-planetary'' disks, and that the disk evolution is coupled to the planet formation process. The MWC 480 results indicate that a proper estimate of the dust temperature and size of the optically thick core are essential to determine the dust emissivity index.
We report the detection of the J=2-1 line of DCO+ in the proto-planetary disk of DM Tau and re-analyze the spectrum covering the 465 GHz transition of HDO in this source, recently published by ...Ceccarelli et al. (2005). A modelling of the DCO+ line profile with the source parameters derived from high resolution HCO+ observations yields a DCO+/HCO+ abundance ratio of about 0.004, an order of magnitude smaller than that derived in the low mass cores. The re-analysis of the 465 GHz spectrum, using the proper continuum flux (0.5 Jy) and source systemic velocity (6.05 km/s), makes it clear that the absorption features attributed to HDO and C6H are almost certainly unrelated to these species. We show that the line-to-continuum ratio of an absorption line in front of a Keplerian disk can hardly exceed the ratio of the turbulent velocity to the projected rotation velocity at the disk edge, unless the line is optically very thick (tau > 10 000). This ratio is typically 0.1-0.3 in proto-planetary disks and is about 0.15 in DM Tau, much smaller than that for the alleged absorption features. We also show that the detection of H2D+ in DM Tau, previously reported by these authors, is only a 2-sigma detection when the proper velocity is adopted. So far, DCO+ is thus the only deuterated molecule clearly detected in proto-planetary disks.
We determined a transcriptional profile specific for clonal stromal mesenchymal stem cells from adult and fetal hematopoietic sites. To identify mesenchymal stem cell-like stromal cell lines, we ...evaluated the adipocytic, osteoblastic, chondrocytic, and vascular smooth muscle differentiation potential and also the hematopoietic supportive (stromal) capacity of six mouse stromal cell lines from adult bone marrow and day 14.5 fetal liver. We found that two lines were quadripotent and also supported hematopoiesis, BMC9 from bone marrow and AFT024 from fetal liver. We then ascertained the set of genes differentially expressed in the intersection set of AFT024 and BMC9 compared with those expressed in the union set of two negative control lines, 2018 and BFC012 (both from fetal liver); 346 genes were upregulated and 299 downregulated. Using Ingenuity software, we found two major gene networks with highly significant scores. One network contained downregulated genes that are known to be implicated in osteoblastic differentiation, proliferation, or transformation. The other network contained upregulated genes that belonged to two categories, cytoskeletal genes and genes implicated in the transcriptional machinery. The data extend the concept of stromal mesenchymal stem cells to clonal cell populations derived not only from bone marrow but also from fetal liver. The gene networks described should discriminate this cell type from other types of stem cells and help define the stem cell state.
The goal of our study was to identify a subset of genes commonly expressed in Side Populations (SP), isolated by Hoechst staining followed by flow cytometry, from adult mouse bone marrow, male adult ...germinal cells, muscle primary culture, and mesenchymal cells. These SP cells have been proposed to be a “stem-like” population and are used here as a “model” that may reveal mechanisms which would be relevant for a better understanding of stem cell properties.
Transcriptional profiles for SP and the more differentiated non-SP cells isolated from the four tissues were compared by hybridization on microarray using a common external reference. Among the 503 genes differentially expressed, which discriminate SP and non-SP cells in all the tissues, the genes upregulated in SP cells are implicated in the quiescent status of the cells, the maintenance of their pluripotency and the capacity to undergo asymmetric division. These genes may be responsible for the decision for self-renewal of these cells, whereas the repression of lineage-affiliated genes in SP cells could be responsible for their undifferentiated state.
These genes, acting in concert, may be the key players that mediate the mechanisms that control stem cell functions, and our results suggest that we have identified common “stemness functions” of these “stem-like” cells.
