Small non-coding microRNAs (miRNAs) are implicated in gene regulation, including those involved in coronary artery disease (CAD). Our aim was to identify whether specific serum miRNAs present in the ...circulating lipoproteins (Lp) are associated with stable or vulnerable CAD patients. A cardiovascular disease-focused screening array was used to assess miRNAs distribution in sera collected from 95 CAD patients: 30 with stable angina (SA), 39 with unstable angina (UA), 26 at one month after myocardial infarction (MI) and 16 healthy control subjects. We found that miR-486, miR-92a and miR-122 presented the highest expression in CAD sera. These miRNA together with miR-125a, miR-146a and miR-33a were further individually analyzed by TaqMan assays. The results were consistent with PCR-array screening data that all of these miRNAs were significantly increased in CAD patients compared to controls. Using a binary logistic regression model, we established that miR-486 and miR-92a in association with some high-density lipoprotein (HDL) components can designate vulnerable CAD patients. Further, all classes of Lp were isolated from sera by density gradient ultracentrifugation. Analysis of the selected miRNAs in each Lp class showed that they were associated mainly with HDL, miR-486 and miR-92a having the highest levels. In UA and MI patients, miR-486 prevailed in HDL2, while miR-92a prevailed in HDL3, and their levels discriminate between stable and vulnerable CAD patients. We identified two circulating miRNAs that in association with some lipid metabolism biomarkers can be used as an additional tool to designate vulnerable CAD patients.
We determine the low-redshift field galaxy stellar mass function (GSMF) using an area of 143 deg2 from the first three years of the Galaxy And Mass Assembly (GAMA) survey. The magnitude limits of ...this redshift survey are r < 19.4 mag over two-thirds and 19.8 mag over one-third of the area. The GSMF is determined from a sample of 5210 galaxies using a density-corrected maximum volume method. This efficiently overcomes the issue of fluctuations in the number density versus redshift. With H
0= 70 km s−1 Mpc−1, the GSMF is well described between 108 and 1011.5 M⊙ using a double Schechter function with
,
, α1=−0.35,
and α2=−1.47. This result is more robust to uncertainties in the flow-model corrected redshifts than from the shallower Sloan Digital Sky Survey main sample (r < 17.8 mag). The upturn in the GSMF is also seen directly in the i-band and K-band galaxy luminosity functions. Accurately measuring the GSMF below 108 M⊙ is possible within the GAMA survey volume but as expected requires deeper imaging data to address the contribution from low surface-brightness galaxies.
In this paper, likelihood-based algorithms are explored for linear digital modulation classification. Hybrid likelihood ratio test (HLRT)- and quasi HLRT (QHLRT)- based algorithms are examined, with ...signal amplitude, phase, and noise power as unknown parameters. The algorithm complexity is first investigated, and findings show that the HLRT suffers from very high complexity, whereas the QHLRT provides a reasonable solution. An upper bound on the performance of QHLRT-based algorithms, which employ unbiased and normally distributed non-data aided estimates of the unknown parameters, is proposed. This is referred to as the QHLRT-Upper Bound (QHLRT-UB). Classification of binary phase shift keying (BPSK) and quadrature phase shift keying (QPSK) signals is presented as a case study. The Cramer-Rao Lower Bounds (CRBs) of non-data aided joint estimates of signal amplitude and phase, and noise power are derived for BPSK and QPSK signals, and further employed to obtain the QHLRT-UB. An upper bound on classification performance of any likelihood-based algorithms is also introduced. Method-of-moments (MoM) estimates of the unknown parameters are investigated and used to develop the QHLRT-based algorithm. Classification performance of this algorithm is compared with the upper bounds, as well as with the quasi Log-Likelihood Ratio (qLLR) and fourth-order cumulant based algorithms.
Airborne lidar (Light Detection And Ranging) is a proven technology that can be used to accurately assess aboveground forest biomass and bio-energy feedstocks. The overall goal of this study was to ...develop a method for assessing aboveground biomass and component biomass for individual trees using airborne lidar data in forest settings typical for loblolly pine stands (
Pinus taeda L.) in the southeastern United States. More specific objectives included: (1) assessing the accuracy of estimating diameter at breast height (dbh) for individual pine trees using lidar-derived individual tree measurements, such as tree height and crown diameter, and (2) investigating the use of lidar-derived individual tree measurements with linear and nonlinear regression to estimate per tree aboveground biomass. In addition, the study presents a method for estimating the biomass of individual tree components, such as foliage, coarse roots, stem bark, and stem wood, as derived quantities from the aboveground biomass prediction. A lidar software application, TreeVaW, was used to extract forest inventory parameters at individual tree level from a lidar-derived canopy height model. Lidar-measured parameters at individual tree level, such as height and crown diameter, were used with regression models to estimate dbh, aboveground tree biomass, and tree-component biomass. Field measurements were collected for 45 loblolly pine trees over 0.1- and 0.01-acre plots. Linear regression models were able to explain 93% of the variability associated with individual tree biomass, 90% for dbh, and 79–80% for components biomass.
With the advent of low-cost and high-resolution digital cameras, and sophisticated photo editing software, digital images can be easily manipulated and altered. Although good forgeries may leave no ...visual clues of having been tampered with, they may, nevertheless, alter the underlying statistics of an image. Most digital cameras, for example, employ a single sensor in conjunction with a color filter array (CFA), and then interpolate the missing color samples to obtain a three channel color image. This interpolation introduces specific correlations which are likely to be destroyed when tampering with an image. We quantify the specific correlations introduced by CFA interpolation, and describe how these correlations, or lack thereof, can be automatically detected in any portion of an image. We show the efficacy of this approach in revealing traces of digital tampering in lossless and lossy compressed color images interpolated with several different CFA algorithms.
Blood-brain barrier alterations in ageing and dementia Popescu, Bogdan O; Toescu, Emil C; Popescu, Laurenţiu M ...
Journal of the neurological sciences,
08/2009, Letnik:
283, Številka:
1
Journal Article, Conference Proceeding
Recenzirano
Abstract The current pathogenic scenarios of different types of dementia are based on a number of common mechanisms of neurodegeneration, such as accumulation of abnormal proteins (within or outside ...cells), mitochondrial dysfunction and oxidative stress, calcium homeostasis dysregulation, early synaptic disconnection and late apoptotic cell death. Ageing itself is associated with mild cognitive deterioration, probably due to subtle multifactorial changes resulting in a global decrease of a functional brain reserve. Increased age is a risk factor for neurodegeneration and key pathological features of dementia can also be found in aged brains. One of the underexplored brain structures in ageing and dementia is the blood-brain barrier (BBB), a complex cellular gate which regulates tightly the transport of molecules into and from the central nervous system. Disruption of this barrier is now increasingly documented not only in brain vascular disease but also in ageing and neurodegenerative disorders. To date, such evidence points mainly at an association between various dementia forms and disruption of the BBB. But, in reviewing such results, and taking into account the exquisite sensitivity of neuronal function to the composition of the interstitial brain fluid (IBF), which is regulated by the BBB, we would like to propose the existence of a possible causal link between alterations of BBB and conditions associated with cognitive decline.
Signaling through mitogen-activated protein kinases (MPKs) cascades is a complex and fundamental process in eukaryotes, requiring MPK-activating kinases (MKKs) and MKK-activating kinases (MKKKs). ...However, to date only a limited number of MKK-MPK interactions and MPK phosphorylation substrates have been revealed. We determined which Arabidopsis thaliana MKKs preferentially activate 10 different MPKs in vivo and used the activated MPKs to probe high-density protein microarrays to determine their phosphorylation targets. Our analyses revealed known and novel signaling modules encompassing 570 MPK phosphorylation substrates; these substrates were enriched in transcription factors involved in the regulation of development, defense, and stress responses. Selected MPK substrates were validated by in planta reconstitution experiments. A subset of activated and wild-type MKKs induced cell death, indicating a possible role for these MKKs in the regulation of cell death. Interestingly, MKK7- and MKK9-induced death requires Sgt1, a known regulator of cell death induced during plant innate immunity. Our predicted MKK-MPK phosphorylation network constitutes a valuable resource to understand the function and specificity of MPK signaling systems.
Despite its critical importance to carbon storage modeling, forest vertical structure remains poorly characterized over large areas. Canopy height estimates from current satellite missions such as ...ICESat-2 (Ice, Cloud, and land Elevation Satellite-2) offer promise to close this knowledge gap, but their validation is critically important to inform their measurement uncertainties and scientific utility. Using existing airborne laser scanning (ALS) data, the agreement of a variety of terrain and aboveground canopy height metrics including summary height statistics and percentiles, from ICESat-2’ Land, Water and Vegetation Elevation product (ATL08) product was assessed in 12 sites across six major biomes in the United States. The agreement between ATL08 and ALS heights was assessed using the mean bias (Bias, ATL08 – ALS), the mean absolute error (MAE) and their percent equivalents, percent bias (pBias) and percent MAE (pMAE), respectively. In general, the agreement between ATL08 and ALS terrain heights was high (Bias 0.18 m, pBias 0.1%) while canopy heights showed lower agreement (Bias −1.71 m, pBias −15.9%). Analyses by biome, time of acquisition and beam strength of the ICESat-2 photon data also showed generally higher agreement for ATL08 terrain than canopy heights. Analyses also showed the performance of ATL08 heights varied with canopy cover with ATL08 terrain heights showing the best agreement when canopy cover was between 40 and 70% while the best performance for ATL08 canopy heights was observed when canopy cover was greater than 80%. This observation, coupled with analyses by biome, indicate that ATL08 canopy heights are more suitable in relatively dense canopy environments such as conifer and broadleaf forests than relatively sparse environments such a temperate grassland and Savannas. Higher level canopy height percentiles (95th and 98th) showed higher agreement (mean Bias −12.5%) with ALS heights than lower percentiles (minimum, 25th, mean pBias ~39.2%). These findings indicate that ATL08 canopy heights show more promise for routine canopy height characterization using the 95th and 98% percentiles but is limited in characterizing intermediate vertical structure. The observed performance differences between ATL08 terrain and canopy heights are attributed to differences in photon sampling rates over terrain and canopy surfaces which, compounded with background noise in ICESat-2 photon data, led to different effectiveness for ATL08 processing routines in filtering terrain and off-terrain points. This assessment of the impact of a variety of factors provides the vegetation community with an understanding of the capabilities and limitations of height estimates from the ICESat-2 ATL08 product.
•Assessed ICESat-2’ ATL08 terrain and canopy heights across multiple sites in the US.•Evaluated ATL08 heights by site, biome, acquisition time and beam strength.•ATL08 terrain heights agreed closely with airborne lidar data across sites/factors.•ATL08 canopy heights varied widely compared to lidar across sites or factors.•These results inform usability of ATL08 data across a variety of factors.
Using the complete Galaxy and Mass Assembly I (GAMA-I) survey covering ∼142 deg2 to r
AB= 19.4, of which ∼47 deg2 is to r
AB= 19.8, we create the GAMA-I galaxy group catalogue (G3Cv1), generated ...using a friends-of-friends (FoF) based grouping algorithm. Our algorithm has been tested extensively on one family of mock GAMA lightcones, constructed from Λ cold dark matter N-body simulations populated with semi-analytic galaxies. Recovered group properties are robust to the effects of interlopers and are median unbiased in the most important respects. G3Cv1 contains 14 388 galaxy groups (with multiplicity ≥2), including 44 186 galaxies out of a possible 110 192 galaxies, implying ∼40 per cent of all galaxies are assigned to a group. The similarities of the mock group catalogues and G3Cv1 are multiple: global characteristics are in general well recovered. However, we do find a noticeable deficit in the number of high multiplicity groups in GAMA compared to the mocks. Additionally, despite exceptionally good local spatial completeness, G3Cv1 contains significantly fewer compact groups with five or more members, this effect becoming most evident for high multiplicity systems. These two differences are most likely due to limitations in the physics included of the current GAMA lightcone mock. Further studies using a variety of galaxy formation models are required to confirm their exact origin. The G3Cv1 catalogue will be made publicly available as and when the relevant GAMA redshifts are made available at http://www.gama-survey.org.
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