Glioblastoma multiforme (GBM) is the most common and aggressive glioma with the poorest survival. Use of biomarkers for screening patients with GBM may be used to modify treatments and improve ...outcomes. The level of human telomerase (hTERT) expression is an independent predictor of outcome of many cancers, and a functional variant of hTERT MNS16A (shorter tandem repeats or short S allele) is associated with increased hTERT mRNA expression. We investigated whether hTERT MNS16A variant genotype predicted survival in GBM patients.
We genotyped hTERT MNS16A in 299 GBM patients using polymerase chain reaction and determined hTERT genotype by classifying the DNA band of 243 or 272 base pairs (bp) as S allele and 302 or 333 bp as long (L) allele. We compared overall survival using Kaplan-Meier estimates and equality of survival distributions using the log-rank test, and we computed univariate and multivariate Cox proportional hazards models to estimate the effects of selected variables.
Overall survival differed significantly by hTERT MNS16A genotype, with median survivals of 25.1, 14.7, and 14.6 months for the SS, SL, and LL genotypes, respectively. Compared with the SS genotype, the hazard ratios for the SL and LL genotypes were 1.69 and 1.87, respectively, after adjustment for other factors. Multivariate Cox regression analysis showed an independent statistically significant association between the hTERT MNS16A variant genotype and outcome.
A functional hTERT MNS16A genotype is a potential biomarker for assessment of survival outcome of GBM. Larger studies are needed to verify these findings.
Abstract
In recommender systems, our main task is to predict the rating of a new product from the authorized user and then return the best rating for the particular item and this technique reduced ...the existing prediction error rate. Our proposed system is user-item rating matrix prediction based on Synergetic filtering techniques and this technique is more efficiency compared to other technique. Proposed System are providing personalized recommendation for help the users accord with information overburden problem. However, the techniques are the data insufficiency of the user-item rating matrix underlying for brand-new items and users are severely affected by Synergetic filtering technique. Since the character of common links and items between more accessible by the users in the Internet and this paper exploits the common links of users and the character of items to overcome the existing problems and to ease the rating insufficient effect. However they may need excessive computational moment, and they often accost the insufficient problem which negatively modify the ability of the system. Specifically, we initially propose a Kernel-based Attribute-aware Self adaptation and multi thresholding model to blend the character information of items into matrix factorization and then introduce self-adaptation and multi thresholding. KASM can find the indefinite interactions among characters, users, and items, which reduce the rating insufficient effect for brand-new items by nature. In this paper we suggest a quick recommendation algorithm based on self-adaptation and multi thresholding. Self-adaptation in its genuine meaning is a state-of-the-art method to alter the setting of control specification. It is called self-adaptive because the algorithm controls the setting of these specification itself sink them into a distinctive genome and emerging them. It is construct to deal with the specified drawbacks and enhance the prediction quality. Extended analysis on two real world data sets establish that our proposed method can attain necessarily improve performance than other state-of the-art-methods. In this method we get the accuracy rate of predicting user rating will be 95%
•A new measure of collaborative intelligence (CI) in the knowledge based service (KBS).•CI-based models for finding the best collaborators in collaborative networks.•Customizable decision making ...process in the collaborative network optimization tool.•Decreased avg. service cost in KBS close to 50% with high CI suggested by the models.
E-services are provided by several web-enabled companies. While some of them are competitive in nature, others are collaborative. There are few methodologies currently existing for measuring the value of e-collaboration among these e-service providers. The objective of this research is to measure collaborative intelligence (CI) in the knowledge based service (KBS) industry and to identify measures for finding the best collaborators during the formation and functioning stages of collaborative networks. The model developed in this research, CIMK (collaborative intelligence measure of KBS), measures CI by multi-objective optimization on parameters for collaboration, and suggests optimal operating points for various clients with greater flexibility. CIMK allows decision makers to customize the model based on their knowledge of the industry. Then, the CNOA (Collaborative Network Optimization Algorithm) is applied to select the best providers for requests based on their CI levels. CNOA has been implemented over a HUB-CI (HUB with CI) platform, which is a next generation collaboration support system developed at Purdue University. Three analytic experiments are designed and performed to validate the models (1) in terms of usability, (2) to compare the CIMK with alternative methods, and (3) to find the relative advantages of the CIMK model. The results of the experiments indicate that the average service cost can decrease by close to 50% when operating points with high CI, suggested by CIMK model, are implemented. The CI level computed by CIMK is successfully used as a decision parameter for on-going matching e-service providers to different requests.
We describe the first report of temporally related cases of Bordetella holmesii bacteremia. Demographic and clinical data were collected through chart abstraction and case-patient interviews. ...Twenty-two cases were identified from 6 states. Symptom onset dates ranged from April 2010 to January 2011. Median age of patients was 17.1 years and 64% had functional or anatomic asplenia. Pulsed-field gel electrophoresis profiles of a sample of isolates were identical. These cases occurred during a peak in pertussis outbreaks with documented cases of B. holmesii/Bordetella pertussis respiratory coinfection; whether there is a link between B. holmesii respiratory and bloodstream infection is unknown.
We describe an efficient high-throughput method for accurate DNA sequencing of entire cDNA clones. Developed as part of our involvement in the Mammalian Gene Collection full-length cDNA sequencing ...initiative, the method has been used and refined in our laboratory since September 2000. Amenable to large scale projects, we have used the method to generate >7 Mb of accurate sequence from 3695 candidate full-length cDNAs. Sequencing is accomplished through the insertion of Mu transposon into cDNAs, followed by sequencing reactions primed with Mu-specific sequencing primers. Transposon insertion reactions are not performed with individual cDNAs but rather on pools of up to 96 clones. This pooling strategy reduces the number of transposon insertion sequencing libraries that would otherwise be required, reducing the costs and enhancing the efficiency of the transposon library construction procedure. Sequences generated using transposon-specific sequencing primers are assembled to yield the full-length cDNA sequence, with sequence editing and other sequence finishing activities performed as required to resolve sequence ambiguities. Although analysis of the many thousands (22 785) of sequenced Mu transposon insertion events revealed a weak sequence preference for Mu insertion, we observed insertion of the Mu transposon into 1015 of the possible 1024 5mer candidate insertion sites.
Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC ...clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.
Antibody-drug conjugates (ADCs) are potent cytotoxic drugs linked to antibodies through chemical linkers, and allow specific targeting of drugs to neoplastic cells. The expression of CD22 is limited ...to B-cells, and we show that CD22 is expressed on the vast majority of non-Hodgkin's lymphomas (NHLs). An ideal target for an ADC for the treatment of NHL would have limited expression outside the B-cell compartment and be highly effective against NHL. We generated an ADC consisting of a humanized anti-CD22 antibody conjugated to the anti-mitotic agent maytansine with a stable linker (anti-CD22-MCC-DM1). Anti-CD22-MCC-DM1 was broadly effective in in vitro killing assays on NHL B-cell lines. We did not find a strong correlation between in vitro potency and CD22 surface expression, internalization of ADC or sensitivity to free drug. We show that anti-CD22-MCC-DM1 was capable of inducing complete tumor regression in NHL xenograft mouse models. Further, anti-CD22-MCC-DM1 was well tolerated in cynomolgus monkeys and substantially decreased circulating B-cells as well as follicle size and germinal center formation in lymphoid organs. These results suggest that anti-CD22-MCC-DM1 has an efficacy, safety and pharmacodynamic profile that support its use as a treatment for NHL.
A solvent-free mechanochemical route for the preparation of poly(2,5-dimethoxyaniline) hydrochloride nanostructures is developed and reported in the article. High conductivity, good crystallinity, ...and nanostructured morphology are observed for the prepared polymer. This polymeric powder is utilized as a cathode material in hybrid supercapacitor and its electrochemical performance is evaluated and discussed in this short report. The maximum specific capacitance of the poly(2,5-dimethoxyaniline) hydrochloride/activated carbon hybrid supercapacitor is found to be 125 F g
−1
at 1 mA cm
−2
current density. The cell delivers a specific energy as high as 50 Wh kg
−1
at a specific power of 97 W kg
−1
and also exhibits an excellent cycle performance with more than 99% coulombic efficiency and the maintenance of 85% of its initial capacitance after 1,000 cycles.
A continuous feedback control approach for real-time scheduling of discrete events is presented in this paper motivated by the need for control theoretic techniques to analyze and design such systems ...in distributed manufacturing applications. These continuous feedback control systems exhibit highly nonlinear and discontinuous dynamics. Specifically, when the production demand in the manufacturing system exceeds the available resource capacity then the control system “chatters” and exhibits sliding modes. This sliding mode behavior is advantageously used in the scheduling application by allowing the system to visit different schedules within an infinitesimal region near the sliding surface. In this paper, an analytical model is developed to characterize the sliding mode dynamics. This model is then used to design controllers in the sliding mode domain to improve the effectiveness of the control system to “search” for schedules with good performance. Computational results indicate that the continuous feedback control approach can provide near-optimal schedules and that it is computationally efficient compared to existing scheduling techniques.
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