The CRISPR/Cas9 system is a powerful tool for the treatment of infectious diseases. In our previous study, we knocked out the Bombyx mori nucleopolyhedrovirus (BmNPV) key genes and BmNPV‐dependent ...host factor to generate transgenic antiviral strains. To further expand the range of target genes for BmNPV and more effectively prevent and control pathogenic infections, we performed gene editing and antiviral analysis by constructing a target‐directed baculovirus early transcriptional activator immediate early‐0 (ie‐0) and 2 (ie‐2) transgenic silkworm line. We hybridized it with Cas9 transgenic line to produce a double‐positive transgenic Cas9(+)/sgIE0‐sgIE2(+) line that could activate the CRISPR gene editing system. We first demonstrated that the system is capable of efficiently editing target genes and resulting in fragment deletions in the BmNPV genome. Survival rate of the transgenic Cas9(+)/sgIE0‐sgIE2(+) line reached 65% after inoculation with 1 × 106 occlusion bodies/larva. Molecular analysis showed that BmNPV DNA replication and viral gene expression level in the transgenic Cas9(+)/sgIE0‐sgIE2(+) line were significantly inhibited compared with the control Cas9(−)/sgIE0‐sgIE2(−) line. These results indicated that IE‐0 and IE‐2, as baculovirus early transcriptional activators, can be used as target sites for gene therapy and that multigene editing could expand the range of target sites for research to create silkworm resistance breeds.
Highlights • β-DG is cleaved by MMP-2/-9 in mouse brains after pMCAO. • MMP-2/-9-mediated β-DG cleavage disturbs AQP4 polarization after pMCAO. • MMP-2/-9 inhibition influences cytotoxic and ...vasogenic edema after pMCAO.
Urban Functional Zone (UFZ) identification facilitates the understanding of urban systems, which are complex and huge, and helps promote sustainable urban development. Existing studies on UFZ ...identification with Points of Interests (POIs) have focused much on more accurately extracting functional semantics, but ignored the fine delineation of UFZs in the spatial domain. The fine delineation of the spatial units of UFZs is also a key issue in UFZ identification. Since the sizes of UFZs can be different in practice, it is difficult to delineate spatially heterogeneous UFZs on a fixed scale. To solve the issue, a novel multi-scale spatial segmentation method was proposed in this study. Through taking the homogeneous socio-economic attributes of UFZs into account, we firstly generated a number of multi-scale spatial units by computing the mixed degree of POIs types, which reflects the mixed functions of each UFZs, using information entropy. Subsequently, we constructed the urban functional corpus of each spatial unit by measuring the spatial distribution pattern of POIs. The Word2Vec model was employed to obtain the semantic embedding vectors of UFZs, following which we adopted cosine distance-based K-means clustering method to group similar UFZs into one cluster. Finally, the enrichment factor was used to help annotate each functional cluster with a specific label. The UFZ identification results were compared with the Baidu e-maps and Baidu street view images for evaluation, and an accuracy of 82.7% was obtained. This study considering the heterogeneous distribution of POIs supports the fine-grained identification of UFZs, providing reference for urban planning.
The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase ...chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.
o solve the technical, economic and environmental problems in the remanufacturing process of decommissioned machine tools, this study proposes a module partition method for remanufacturing retired ...machine tools. Through an analysis of the economic, accuracy, environment-friendly, and functional and physical interactivity principles, this study applies the fuzzy cluster method (FCM) to analyse the modularity of the components to be assembled. It establishes the correlation matrix between the parts, from which a module division scheme is obtained. Finally, a case study is presented to verify the feasibility of the module division method. Keywords decommissioned machine tools; remanufacturing; module partition; fuzzy clustering
To investigate the pharmacist's knowledge about rational use of antimicrobials in Shanxi of China, so as to find out the problems and provide support for the management of antimicrobials.
A ...questionnaire survey was conducted, which included the basic information of the respondents, the basic knowledge about antimicrobial management and the related knowledge about antimicrobial drugs. SPSS 25.0 was used for statistical analysis.
A total of 462 pharmacists were investigated. The average score of the knowledge related to rational use of antimicrobials was 10.49 ± 4.05. It showed that the hospital type, grade, pharmacist's education, professional title and years of experience had effect on the pharmacist's knowledge level about antimicrobial drugs (P < 0.05). Multivariable logistic regression analysis showed that hospital grade and pharmacist's education were the main influencing factors (P < 0.05).
Pharmacists have insufficient knowledge about the rational use of antibacterial drugs. It is essential to strengthen the training in management regulations and application of antibacterial drugs.
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•Helmholtz resonators and perforated panel’s attenuation properties are measured.•CFD simulations of Helmholtz resonators are validated against measurements.•A porous model for CFD simulation of ...perforated plates is presented.•Existing analytical models are compared with CFD models and measured data.•CFD methods are shown to improve the prediction of resultant attenuation.
This paper reports upon developments in the simulation of the passive control of combustion dynamics in industrial gas turbines using acoustic attenuation devices such as Helmholtz resonators and perforated liners.
Combustion instability in gas turbine combustors may, if uncontrolled, lead to large-amplitude pressure fluctuations, with consequent serious mechanical problems in the gas turbine combustor system. Perforated combustor walls and Helmholtz resonators are two commonly used passive instability control devices. However, experimental design of the noise attenuation device is time-consuming and calls for expensive trial and error practice. Despite significant advances over recent decades, the ability of Computational Fluid Dynamics to predict the attenuation of pressure fluctuations by these instability control devices is still not well validated. In this paper, the attenuation of pressure fluctuations by a group of multi-perforated panel absorbers and Helmholtz resonators are investigated both by experiment and computational simulation. It is demonstrated that CFD can predict the noise attenuation from Helmholtz resonators with good accuracy. A porous material model is modified to represent a multi-perforated panel and this perforated wall representation approach is demonstrated to be able to accurately predict the pressure fluctuation attenuation effect of perforated panels. This work demonstrates the applicability of CFD in gas turbine combustion instability control device design.
This study aims to quantitatively summarize the evidence for CKMM gene NcoI (A/G, rs8111989) polymorphism and sport performance in elite athletes.
The PubMed, EMBASE, Weipu, CNKI and Wanfang database ...were searched for eligible studies. These studies contained available genotype frequencies of A/G in elite athletes and general controls, and odds ratio (OR) with 95% confidence interval (CI) was used to assess the strength of this association.
In this meta-analysis, 15 articles on CKMM gene were identified. We conducted stratified analyses according to ethnicity and sports category, and we found that the power athletes had a higher frequency of the G allele (Asian: OR=1.560, 95% CI 1.058∼2.299, P=0.025) and the GG genotype (Overall: OR=1.732, 95% CI 1.211∼2.479, P=0.003. Asians: OR=2.154, 95% CI 1.077∼4.309, P=0.030. Caucasians: OR=1.599, 95% CI 1.049∼2.436, P=0.029) compared to controls. Moreover, in sports that require both endurance and explosive power, athletes also had a higher frequency of the GG genotype (Overall: OR=1.371, 95% CI 1.067∼1.763, P=0.014.). But there was no significant difference for the endurance athletes in any genotype.
This meta-analysis suggests that CKMM gene rs8111989 polymorphism GG genotype is associated with sport performance in power sports but not in endurance sports.
Cette étude vise à récapituler les preuves d’un lien entre le polymorphisme NcoI (A/G, rs8111989) du gène CKMM et les performances sportives chez les athlètes de haut niveau.
Les bases de données PubMed, EMBASE, Weipu, CNKI et Wanfang ont été consultées à le recherche d’études éligibles. Les études contenant les fréquences génotypiques disponibles de A/G chez les athlètes d’élite et les sujets témoins généraux, les odds ratios (OR) avec un intervalle de confiance (IC) à 95 % a été incluses dans l’étude.
Dans cette méta-analyse, 15 articles sur le gène CKMM ont été identifiés. Nous avons effectué des analyses stratifiées en fonction de l’appartenance ethnique et de la catégorie sportive, et nous avons constaté que les athlètes de sports de puissance présentaient une fréquence plus élevée de l’allèle G (asiatique: OR=1,560, IC à 95 % 1,058 ∼ 2,299, p=0,025) et du génotype GG (dans l’ensemble : OR=1,732, IC 95 % 1,211 ∼ 2,479, p=0,003. Asiatiques : OR=2,154, IC 95 % 1,077 ∼ 4,309, p=0,030. Caucasiens : OR=1,599, IC 95 % 1,049 ∼ 2,436, p=0,029) par rapport aux témoins. De plus, dans les sports qui impliquent à la fois l’endurance et la puissance explosive, les athlètes avaient également une fréquence plus élevée du génotype GG (globalement : OR=1,371, IC à 95 % 1,067 ∼ 1,763, p=0,014). Cependant, il n’y avait aucune différence significative pour les athlètes d’endurance quel que soit le génotype.
Notre méta-analyse suggère que le polymorphisme du gène CKMM rs8111989 génotype GG est associé à la performance sportive dans les sports de puissance, mais pas dans les sports d’endurance.
Abstract Background and aims Lactotripeptides (LTPs, including IPP and VPP) have held promise in the framework of lifestyle modification for prevention and control of hypertension – a cardiovascular ...risk factor, as LTPs are reported to have an inhibitory effect on angiotensin-converting enzyme. While the number of clinical trials to test the efficacy of LTP continues to increase, the results have been inconsistent, especially in the last few years. The purpose of the present meta-analysis is to precisely estimate the pooled mean effect of LTPs on conventional blood pressure (BP) generally and on 24-h ambulatory BP (ABP) particularly, as well as the change of BP in relation to baseline BP, race, and study design, to better reflect the evolving field. Data synthesis In general analysis of 24 studies with 28 trials on 1919 human subjects, there are small reductions in both systolic BP (SBP) and diastolic BP (DBP) with the pooled mean effects of 1.66 (95% confidence interval (CI): −2.48 and −0.84) and 0.76 mmHg (−1.31 and −0.20) in response to LTP administration. In analysis of 24-h ABP response to LTP intervention, the reductions of SBP and DBP are 1.30 (−2.49 and −0.11) and 0.57 mmHg (−1.49 and 0.35). In subgroup analysis, the anti-hypertensive efficacy appears to be related to baseline BP, ethnic differences, treatment duration and double versus not double-blind design. Conclusions The present findings indicate that the BP-lowering effect of LTP is statistically significant, though small in magnitude. More clinical investigations (especially randomized double-blind trials with ABP) are warranted to determine the anti-hypertensive efficacy of LTP conclusively.
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