B-cell stimulation for the purpose of evoking an effective neutralizing humoral immune response is a surface phenomenon that is exquisitely specific to antigen conformation. Consequently, successful ...delivery of antigen, such as would be desired in a vaccine, entails preservation of an antigen's apparent native surface (conformational) properties. Prior to testing the actual vaccinating efficacy of delivered antigens, the surface properties could be assessed through a variety of in vitro and in vivo assays in which the measurement standard would be the properties of the antigens in their native state (whole virus). Using surface modified nanocrystalline carbon and calcium-phosphate ceramic particulates (carbon ceramics and brushite), we evaluated the surface activity of immobilized non-nuclear material extracted from HIV-1. Physical characterization showed that the particles with immobilized antigen ("HIV decoys") measured 50 nm in diameter (HIV = 50-100 nm) and exhibited the same zeta potentials as whole (live) HIV. In vitro testing showed that the HIV decoys were recognized by both conformationally nonspecific and specific monoclonal antibodies, were recognized by human IgG from HIV antibody-positive patients, and could promote surface agglomeration among malignant T-cells similar to live HIV. Last, in vivo testing in three vaccinated animal species showed that the HIV decoys elicited humoral and cellular immune responses similar to that evoked by whole (live) HIV.
In research animals, the immune adjuvant activity of silicone in eliciting antibodies to associated protein antigens is now well established. In humans, the immune adjuvant activity of silicone ...remains controversial. Clusters of data from various research groups are beginning to define the boundary conditions for future large epidemiological studies. In that spirit, this manuscript reports pilot clinical data from a serological study conducted 3 years ago and reported previously in this journal. Sera from a self-selected symptomatic population of patients with breast implants were assayed for elevated concentrations of antibodies showing binding avidity to silicone surface associated antigens anti-SSAA(x). In that study of 249 patients, two distinct statistically significant subgroups were identified on the basis of the serological assay alone: patients without any elevated anti-SSAA(x) (negative) and patients with elevated anti-SSAA(x) (positive) (p < 0.001). In this study, a clinical survey returned by 226 of those patients was correlated with the previously acquired serological findings. The 11 most common clinical complaints reported by the 199 anti-SSAA(x) negative patients and by the 27 anti-SSAA(x) positive were compared and their frequencies analyzed. The 199 anti-SSAA(x) negative patients, as a group, showed a lower frequency of a variety of signs and symptoms compared to the 27 anti-SSAA(x) positive patients. Statistically significant differences were seen in three of the symptoms: fever, foot pain, and sleep disturbance (p < 0.05). In addition, the syndrome of fever in the absence of local chest pain was a significantly associated with anti-SSAA(x) positivity (p < 0.001).
M est née à 35,5 SA avec un RCIU sévère (PN : 1 200 g, TN : 39,5 cm) de diagnostic anténatal. À 8 heures de vie apparaît une hyperglycémie à 3 g/l avec glycosurie sans cétonurie ni acidose en regard ...d’une insulinémie effondrée. L’hyperglycémie persistant malgré des apports glucidiques faibles, le diagnostic de diabète néonatal est posé et l’insulinothérapie est débutée par voie IV. Le relais par pompe à insuline sous-cutanée avec des apports caloriques élevés (NEDC puis biberons) a permis une équilibration glycémique et une reprise pondérale (poids à 2 mois 1/2 : 2 500 g). L’existence d’une diarrhée chronique fait évoquer une insuffisance pancréatique exocrine confirmée par le dosage de l’élastase fécale effondrée < 15 μg/g (N > 200) et de l’activité chymotrypsique nulle (N < 6 U/g). Une anomalie de développement du pancréas est suspectée qui sera confirmée par les échographies et scanners abdominaux (à 3 mois et à 1 an) qui ne mettent pas en évidence de tissu dans la loge pancréatique. L’insulinothérapie par pompe sous-cutanée associée aux extraits pancréatiques et à une nutrition adaptée ont permis une croissance de rattrapage avec un gain de 3DS de poids et 4DS de taille à l’âge de 1 an. L’équilibre glycémique est satisfaisant (à 1 an, HbA1c : 6 %). L’étude génétique du chromosome 6 chez l’enfant et ses parents ne montre pas d’anomalie impliquée dans le diabète néonatal transitoire. La recherche d’une mutation du facteur de transcription PDX-1 (
pancreatic and duodenal homeobox gene-1) est en cours.
Le diabète néonatal est une pathologie rare (1/400 000 naissances) associée à un RCIU ; il peut être transitoire (associé dans 80 % des cas à une anomalie du chromosome 6 d’origine paternelle) ou permanent (DNP). Cette observation est exceptionnelles de DNP correspond au 12
e cas d’agénésie du pancréas rapporté dans la littérature.
Les schistosomes d'ammaux d'Asie Farhati, K.; de Pecoulas, Ph. Eldin; Rajguru-Kazemi, M. ...
Médecine et maladies infectieuses,
2/1995, Letnik:
25, Številka:
2
Journal Article
Les schistosomes d'animaux d'Asie du Sud-Est se répartissent principalement dans le complexe
S. indicum (
Schistosoma indicum, S. nasale, S. spindale et
S. incognitum) et accessoirement dans le ...complexe
S. japonicum (
S. sinensium). Certains de ces schistosomes restent localisés en Inde alors que d'autres se trouvent dans tout le sous-continent indien. Leur importance en médecine vétérinaire est variable, allant de la parasitose bien tolérée à la maladie grave ou mortelle aux conséquences économiques considérables.
South-East asian schistosomes in animals mostly belong to the
S. indicum complex (
Schistosoma indicum, S. nasale, S. spindale and
S. incognitum) and also to the
S. japonicum one (
S. sinensium). Some of them can be found only in India, when other have spread every where in the Indian sub-continent. Their veterinary implication is variable; from a well-supported disease to a life threatening one which has some important economical repercussions.
To study the combining ability in respect of nine characters in rabi sorghum, the parental material consisting of three male sterile lines and ten restorer lines along with two checks was taken. ...There was considerable amount of genetic variability among the parents for all characters under study. The male sterile lines 53A and 36642A and the restores SPV-489, RS -29, SPV-1277 and RSE-5 were found to be good general combiners while, the crosses 53A x SPV-1277, 36642A x SPV-489, 116A x RS-29 and 36642A x RSE-5 exhibited good SCA effects for grain yield and most of its components. From the above crosses 53A x SPV-1277 was the best specific combination which exhibited higher magnitude of significant positively specific combining ability effects for grain yield and most of its components.
