Development of biocompatible and biodegradable 3D scaffold for biomedical application has been challenging. Herein, the application of a novel nanocomposite 3D scaffold based on poly vinyl alcohol ...and sodium alginate embedded with Ca2+ doped titanium dioxide nanoparticle (PVA+SA/Ca@TiO2) as bioactive material was investigated. The 3D scaffold was prepared using a solvent casting method and characterized using FTIR, SEM, XRD and TGA to study their physiochemical properties. The spectroscopy techniques were used to confirm the formation of the required poly vinyl alcohol and sodium alginate with Ca doped TiO2 nanoparticles fillers interaction by the identification of bands. The Ti-OH bonds and the bonds related to the interconnected network between the inorganic and the organic components from hybrids. The PVA+SA/Ca@TiO2 nanocomposite 3D scaffold was demonstrated good bioactivity to promote nucleation and growth of hydroxyapatite (HAp) in the simulated body fluid. The PVA+SA/Ca@TiO2 nanocomposite 3D scaffold supported the growth and proliferation of 3T3 mouse fibroblast cells with strong proliferation and cell attachment. The biocompatibility and nontoxic character of the 3D scaffold was probed using fibroblast cell with over 90% of cell viability. It offers a potential for cell attachment and captured for biomedical application.
Wound healing dressing based on a natural polymer of gellan gum incorporating zinc oxide nanoparticles and multiwall carbon nanotubes (GG/ZnONP + MWCNT) bionanocomposite film was fabricated via the ...solution casting method. The physicochemical properties of the film were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM). Moreover, the antibacterial properties of the bionanocomposite film were investigated for wound healing applications. The characterization results confirmed the reinforcement of the gellan gum (GG) matrix with zinc oxide nanoparticles (ZnONP) and multiwall carbon nanotubes (MWCNT), as an amorphous GG/ZnONP + MWCNT bionanocomposite film was obtained. SEM morphological analysis shows that the addition of ZnONP and MWCNT nanofillers changed the film microstructure into a sponge-like structure that is more suitable for fluid uptake and thus more useful for wound healing. The GG/ZnONP + MWCNT bionanocomposite film demonstrated good antibacterial activity against all strains tested. Furthermore, macroscopic analysis shows that the wound treated with GG/ZnONP + MWCNT bionanocomposite film recovered completely (100%) in 14 days, compared to pure GG film (90.76%) and negative control (77.40%). As a result, the GG/ZnONP + MWCNT bionanocomposite film could be a promising wound dressing material.
This study investigated the effect of sawdust at 0, 10, 20, 30 and 40 wt% towards physical properties and filtration efficiency of porous clay membrane. Sawdust in various quantities was added into ...water containing clay, polyethylene glycol and sodium silicate and then stirred to form homogenized slurry. The slurry then was casted into Plaster of Paris (PoP) mould. The green body was cut into required sizes, dried and sintered in furnace at 1000 °C. The porosity and density of porous clay was determined by Archimedes principle, while morphology was observed by Hitachi Tabletop Scanning Electron Microscope (TTSEM). The filtration test was examined on a dead-end filtration setup in batch mode operation using nitrogen as carrier gas. Distilled water was filtered and used to determine membrane permeability, while aquaculture wastewater filtration was used to determine the turbidity removal. Turbidity of aquaculture wastewater and effluent (after filtration) were measured using turbidity meter. The results indicated that porosity increased from 38 % to 64 %, while density decreased from 1.5 g/cm3 to 0.8 g/cm3 with increasing amount of sawdust from 0 to 40 wt%. This data was supported by TTSEM which is indicate that the amount of pores increased with increasing amount of sawdust. Permeation of membrane increased from 45289 to 143999 L.h-1.m-2.bar-1 and efficiency of turbidity removal increased from 87 % to 89 % with increasing amount of sawdust from 0 to 40 wt%. As conclusion, 30 wt% was an optimum amount of sawdust which is produced ceramic membrane with good structure integrity, porosity, high permeability and high turbidity removal.
Abstract
The consumption of too much fluoride ions through drinking water can seriously harm human health. Thus fluoride ions need to be removed by the novel and efficient nanomaterials materials ...synthesized via eco-friendly method. The pure and iron-doped hydroxyapatites were synthesized using a simple co-precipitation technique for the removal of fluoride from water. The synthesized materials were characterized by advanced technical tools. The point of zero charge of the materials was determined by the salt addition method. Crystallite size and degree of crystallinity were observed to decrease with the substitution of calcium. However, the surface area and pore volume were found to have enhanced with modification of iron in the apatite. Batch adsorption experimental data were well fitted to pseudo-second order and Langmuir models, which implied that the sorption process is chemisorption through a monolayer on a homogenous surface. The maximum sorption capacities of HA and Fe-HA were found to be 40.46 and 83.86 mg g
−1
, respectively. The thermodynamic data revealed that the adsorption process is endothermic and spontaneous. The regeneration and reuse analysis insured that the materials have good potential for reuse. The adsorption mechanism was inferred as chemisorption through electrostatic interaction and ion exchange. The modification of hydroxyapatite using iron considered as a competent sorbent for the removal of fluoride ions.
In this study, functionalized-MWCNTs with 3-aminopropyltriethoxysilane (APTES) loaded titania nanoparticles (MWCNTs-APTES-TiO2) were prepared to investigate their physicochemical properties and ...photocatalytic activity for methyl orange (MO) degradation. The TiO2 nanoparticles, functionalized-MNCNT and composite powders were characterized by XRD, raman, and TEM. The results obtained proved that titania (TiO2) nanoparticles was successfully loaded on APTES-MWCNTs. For application, MWCNTs-APTES-TiO2 composites were used as photocatalyst for degradation of methyl orange (MO) in aqueous solution under UV light irradiation and the result shows that 87% MO was degraded after 180 min. Kinetic analysis indicated that photocatalytic degradation of MO solution by MWCNTs-APTES-TiO2 obeyed second-order kinetic model (R2 > 0.95), supported by half-life equations and graph. Because of the presence of carbon nanotubes accelerated the degradation of methyl orange due to inhibition of electron-hole recombination, the formation of additional hydroxyl radicals and functional groups of the latter had an inhibitory effect on the degradation of methyl orange. This study suggested the MWCNTs-APTES-TiO2 nanocomposite has considerable potential as environmental photocatalyst due to higher degradation rate as compared to bare TiO2.
Antibacterial Study of Gellan Gum (GG) Film Incorporated Norfloxacin Ismail, Nur Arifah; Amin, Khairul Anuar Mat; Razali, Mohd. Hasmizam
Journal of pure & applied microbiology : an international research journal of microbiology,
06/2019, Letnik:
13, Številka:
2
Journal Article
Recenzirano
Odprti dostop
A series of norfloxacin-loaded films were fabricated by combining with gellan gum (GG) using evaporative casting technique. The films were evaluated in terms of morphological, water vapour ...transmission rates (WVTR), water uptake, release study and antibacterial study. The prepared films contained smooth appearance of cross-section distributed by good film of materials. The results indicated that the film swelling were increased at higher concentration of norfloxacin loaded, however decreased the WVTR properties. All films showed almost 100 % of drug release within 5 - 20 minutes. It was found that, the antibacterial activity was directly proportional to the release rate which is at higher concentration of norfloxacin resulted in stronger antibacterial properties. Keywords: Gellan gum; Film; Antibacterial.
One million cases of skin wounds, either closed or open wounds, necessitate wound treatments to improve the quality of life. In this study, gellan gum biopolymer (Ag@TiO2NRs/GG) hydrogel film with Ag ...loaded TiO2 nanorods was fabricated for wound healing dressing. The wound healing performance of Ag@TiO2WR/GG hydrogel was tested in vitro and in vivo to investigate its ability to regenerate skin tissue. FTIR, XRD, and SEM were used to examine the physical and chemical properties of prepared Ag@TiO2NRs/GG hydrogel film, as well as pure Ag and Ag@TiO2NRs. The FTIR spectra revealed the functional groups of Ag, TiO2NRs, GG, and their interactions. The hydrogel film was in an amorphous form, according to XRD analysis, due to the helical structure of GG and the presence of Ag and TiO2NRs in distinct phases. The SEM image shows agglomeration of Ag particles and elongated TiO2 nanorods, indicating that Ag@TiO2NRs were successfully incorporated onto GG hydrogel film. Human skin fibroblast cells (CRL2522) were used to study the in vitro wound healing of Ag@TiO2NRs/GG hydrogel film for cell viability and proliferation. After 72 h, ∼98,022 cells well−1 were counted, indicating that the Ag@TiO2NRs/GG was biocompatible and non-toxic. In vivo wound healing on Sprague Dawley rats revealed 100% wound healing after 14 days of treatment with Ag@TiO2NRs/GG hydrogel film. On a treated skin wound, ultrasound images revealed a thicker epidermis, clear dermis, and subcutis layer, indicating a positive correlation between wound healing and skin tissue regeneration.
In this research, the g-C
3
N
4
/TiO
2
nanocomposite was prepared by a simple hydrothermal method and was used as photocatalyst for dye degradation. Prior to that, the g-C
3
N
4
/TiO
2
was ...characterized by different analytical techniques such as FTIR, XRD, SEM, and nitrogen gas adsorption. Main functional groups of g-C
3
N
4
/TiO
2
composite are shown in the FTIR spectrum. The XRD pattern reveals that the presence of anatase and rutile phases of TiO
2
as well as layer stacking of conjugated aromatic and in-planar repeating triazine unit of g-C
3
N
4
. The SEM analysis shows the presence of 2D-layered structured of g-C
3
N
4
and agglomerated spherical TiO
2
particles (0D). The 2D/0D g-C
3
N
4
/TiO
2
nanocomposite shows higher photocatalytic activity than pure g-C
3
N
4
and TiO
2
, whereby 100% of MB was degraded under visible light after 2 h. This is attributed to their high surface area which is 273.32 mg
−1
and generation of more effective reactive oxygen species of
∙
OH and
∙
O
2
to degrade MB.
•Hydrothermally synthesised CuO loaded TiO2 nanotubes photocatalyst.•CuO prevented and retarded the electron-hole recombination.•Effectively and efficiently convert CO2 to fuels.
Copper oxide (CuO) ...loaded titanium dioxide (TiO2) nanotube photocatalyst was synthesised using the facile hydrothermal method. The synthesised photocatalyst were characterised by X-ray diffraction (XRD), field emission scanning microscopy (FESEM), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS) to study their physiochemical properties. The photocatalytic activity of the photocatalysts was tested for carbon dioxide (CO2) photoconversion into fuels. CuO loaded TiO2 nanotube demonstrated higher CO2 conversion (100%) than TiO2 nanotube (72.20%) because of the former’s effective separation of photogenerated electron-hole pairs in the presence of CuO.
Biomaterials with exceptional biocompatibility and bioactivity are now pushing the boundaries of bone tissue engineering. In this study, natural Arabic gum biopolymer incorporating titanium dioxide ...nanoparticles (NAG + TiO2NP) nanocomposite film was fabricated. The FTIR and XRD analysis show the presence of functional groups assigned to NAG biopolymers and highly crystalline anatase TiO2NP. Well dispersed TiO2NP can be seen from SEM micrograph suggesting good interaction between TiO2NP filler and NAG biopolymer matrix to enhance the mechanical characteristics of nanocomposite film. The NAG + TiO2NP nanocomposite film exhibited strong bioactivity to form bone-like apatite and promoted the proliferation of MG-63 cells attributed to their excellent biocompatibility and non-toxicity. The NAG + TiO2NP nanocomposite film also displays high antibacterial activity with (36.33 ± 1.53) mm and (27.00 ± 2.00) mm inhibition zone were recorded against Staphylococcus aureus and Escherichia coli. The findings indicate that the NAG + TiO2NP nanocomposite film, with its improved mechanical properties, high swelling capacity, biodegradability, and non-toxicity, shows promise as a viable option for bone tissue regeneration materials.
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