The contamination of pathogenic micro-organisms and heavy metals in drinking water sources poses a serious threat to human health, which raises the demand for efficient water treatments. Herein, ...multi-functional capacitive deionization (CDI) electrodes were developed for the simultaneous decontamination of bacteria and heavy metal contaminants. Polyhexamethylene guanidine (PHMG), an antibacterial polymer, was deposited on the surface of the activated carbon (AC) electrode with the assistance of mussel-inspired polydopamine (PDA) chemistry. The main characterization results proved successful co-deposition of PDA and PHMG on the AC electrode, forming a hydrophilic coating layer in one step. Electrochemical analyses indicated that the AC-PDA/PHMG electrodes presented satisfactory capacitive behaviors, with outstanding salt adsorption capacity and cycling stability. The modified electrodes also exhibit excellent disinfection performance and heavy metal adsorption performance. The bacterial elimination rate of co-deposited electrodes grew along with the increase in the PHMG content. Particularly, AC-PDA/PHMG2 electrodes successfully removed and deactivated 99.11% Escherichia coli and 98.67% Pseudomonas aeruginosa (104 CFU mL–1) in water within 60 min. Furthermore, three flow cells made by AC-PDA/PHMG2 electrodes connected in series achieved efficient removal of salt, heavy metals such as lead and cadmium, and bacteria simultaneously, which indicated that the adsorption performance is significantly improved compared with pristine AC electrodes. These results denote the enormous potential of this one-step prepared multi-functional electrodes for facile and effective water purification using CDI technology.
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•SA/DE@ZIF-8 microspheres with uniform size were prepared by electrostatic spray technology.•SA/DE@ZIF-8 microspheres have porous and ultra-high specific surface area.•Maximum ...adsorption capacity of Cu(II) was 599.07 mg/g.•Composite microspheres provide a carrier for ZIF-8, easy to recycle and reuse.
Diatomite (DE)-based sodium alginate (SA) loaded ZIF-8 composite microspheres were prepared by high-voltage electrostatic spray technology. SA/DE microspheres were prepared by SA and acid-base-regulated silica sol solution. Ultra-small ZIF-8 was grown on SA/DE microspheres by in-situ synthesis method to obtain ultra-high specific surface area composite microspheres. The morphology and particle size of the composite microspheres were analyzed by SEM, and characterized by FT-IR, XRD and TGA. The optimum adsorption efficiency were achieved by a series of conditions optimization. The adsorption of Cu(II) by SA/DE@ZIF-8 conforms to the Langmuir adsorption model, and the theoretical maximum adsorption capacity is 599.07 mg/g. In summary, this study opens up a new strategy for the design of efficient and easily recycled ZIF-8 composite microspheres for water treatment.
Recent findings have shown that circRNA dysregulation was involved in the development of many types of cancer. However, our knowledge of circRNA in oral squamous cell carcinoma (OSCC) remains elusive.
...Here, we explored whether ciRS-7 could function as a ceRNA in promoting metastasis of OSCC via regulating miR-7 activity. The expression levels of ciRS-7 and miR-7 were examined in clinical samples and cell lines by qRT-PCR, and the effects of ectopic expression of ciRS-7 and miR-7 on cell proliferation, migration and invasion were assessed in vitro and in vivo. The effects of ciRS-7 on miR-7 activity were investigated by means of luciferase reporter assay, qRT-PCR and Western blot. In addition, the effects of miR-7 mediated ciRS-7 on the levels of MAPK/AKT signaling proteins were evaluated by Western blot.
We found that ciRS-7 was highly expressed in OSCC tissues and cell lines compared with normal counterparts. Ectopic expression of ciRS-7 significantly promoted OSCC cell proliferation, migration and invasion through in vitro and in vivo. Based on bioinformatics analysis, qRT-PCR, Western blot and luciferase reporter assays, we determined that ciRS-7 functioned as a sponge for miR-7, resulting in attenuation of miR-7 targets RAF-1 and PIK3CD, which are core components of the MAPK/AKT signaling pathways. Moreover, miR-7 correlated with perineural and lymphovascular invasion in OSCC patients. Further experiments demonstrated that ciRS-7 overexpression could attenuate the anti-tumor effects of miR-7 on OSCC cells.
Our results suggested that ciRS-7 can interact directly with miR-7, resulting in upregulation of RAF-1/PIK3CD expression and enhancing metastatic progression of OSCC.
In order to beneficially exploit the scarce wireless spectral resources, spectrum sharing between communication and radar systems has become a promising research topic. However, traditional network ...association strategies may not result in efficient hybrid communication and radar systems. We circumvent this problem by formulating a partially observable Markov decision processes (POMDP) aided network association scheme, where the radar user acts as the primary user (PU), while the cognitive communication user is the secondary user (SU). For maximizing the network throughput, whilst minimizing the interference imposed on the radar user, the communication user is configured for adaptively selecting its underlay or overlay access mode. Moreover, a low-complexity near-optimal reinforcement learning algorithm is proposed for the co-design by considering both its complexity and feasibility. Finally, we quantify the performance of our proposed POMDP based network association scheme.
Person attribute recognition aims to identify the attribute labels from the pedestrian images. Extracting contextual relation from the images and attributes, including the spatial-semantic relations, ...the spatial context and the semantic correlation, is beneficial to enhance the discrimination of the features for recognizing the attributes. Thus, this work proposes a sequence contextual relation learning (SCRL) method to capture these relations. It first embeds the images and attributes into sequences in two branches. Then SCRL flexibly learns the contextual relation from the sequences with the parallel attention model structure, which integrates the inter-attention and intra-attention models. The inter-attention module is utilized to extract the spatial-semantic relations, while the intra-attention is designed to gain the spatial context and the semantic correlation. Both attention modules are comprised of several parallel attention units and each unit can obtain the pairwise relations in one subspace. Therefore, they obtain the relations in multiple subspaces, which can improve the comprehensiveness of the relation learning. Additionally, for the sake of better extraction of spatial-semantic relations, this paper employs connectionist temporal classification (CTC) loss which is capable of driving the network to enforce monotonic alignment between the image and attribute. It can also accelerate the convergence of the network by the algorithm in it. Extensive experiments on five public datasets, i.e., Market-1501 attribute, Duke attribute, PETA, RAP and PA-100K datasets, demonstrate the effectiveness of the proposed method.
The BES1 gene family, an important class of plant-specific transcription factors, play key roles in the BR signal pathway in plants, regulating various development processes. Until now, there has ...been no comprehensive analysis of the BES1 gene family in Brassica napus, and a cross-genome exploration of their origin, copy number changes, and functional innovation in plants was also not available.
We identified 28 BES1 genes in B. napus from its two subgenomes (AA and CC). We found that 71.43% of them were duplicated in the tetraploidization, and their gene expression showed a prominent subgenome bias in the roots. Additionally, we identified 104 BES1 genes in another 18 representative angiosperms and performed a comparative analysis with B. napus, including evolutionary trajectory, gene duplication, positive selection, and expression pattern. Exploiting the available genome datasets, we performed a large-scale analysis across plants and algae suggested that the BES1 gene family could have originated from group F, expanding to form other groups (A to E) by duplicating or alternatively deleting some domains. We detected an additional domain containing M4 to M8 in exclusively groups F1 and F2. We found evidence that whole-genome duplication (WGD) contributed the most to the expansion of this gene family among examined dicots, while dispersed duplication contributed the most to expansion in certain monocots. Moreover, we inferred that positive selection might have occurred on major phylogenetic nodes during the evolution of plants.
Grossly, a cross-genome comparative analysis of the BES1 genes in B. napus and other species sheds light on understanding its copy number expansion, natural selection, and functional innovation.
The analysis at the single-molecule level of proteins and their interactions can provide critical information for understanding biological processes and diseases, particularly for proteins present in ...biological samples with low copy numbers. Nanopore sensing is an analytical technique that allows label-free detection of single proteins in solution and is ideally suited to applications, such as studying protein–protein interactions, biomarker screening, drug discovery, and even protein sequencing. However, given the current spatiotemporal limitations in protein nanopore sensing, challenges remain in controlling protein translocation through a nanopore and relating protein structures and functions with nanopore readouts. Here, we demonstrate that supercharged unstructured polypeptides (SUPs) can be genetically fused with proteins of interest and used as molecular carriers to facilitate nanopore detection of proteins. We show that cationic SUPs can substantially slow down the translocation of target proteins due to their electrostatic interactions with the nanopore surface. This approach enables the differentiation of individual proteins with different sizes and shapes via characteristic subpeaks in the nanopore current, thus facilitating a viable route to use polypeptide molecular carriers to control molecular transport and as a potential system to study protein–protein interactions at the single-molecule level.
Testicular dysgenesis syndrome in male neonates manifests as cryptorchidism and hypospadias, which can be mimicked by in utero phthalate exposure. However, the underlying phthalate mediated mechanism ...and therapeutic effects of taxifolin remain unclear. Di-(2-ethylhexyl) phthalate (DEHP) is the most abundantly used phthalate and can induce testicular dysgenesis syndrome in male rats. To explore the mechanism of DEHP mediated effects and develop a therapeutic drug, the natural phytomedicine taxifolin was used. Pregnant Sprague-Dawley female rats were daily gavaged with 750 mg/kg/d DEHP or 10 or 20 mg/kg/d taxifolin alone or in combination from gestational day 14 to 21, and male pup's fetal Leydig cell function, testicular MDA, and antioxidants were examined. DEHP significantly reduced serum testosterone levels of male pups, down-regulated the expression of SCARB1, CYP11A1, HSD3B1, HSD17B3, and INSL3, reduced the cell size of fetal Leydig cells, decreased the levels of antioxidant and related signals (SOD2 and CAT, SIRT1, and PGC1α), induced abnormal aggregation of fetal Leydig cells, and stimulated formation of multinucleated gonocytes and MDA levels. Taxifolin alone (10 and 20 mg/kg/d) did not affect these parameters. However, taxifolin significantly rescued DEHP-induced alterations. DEHP exposure in utero can induce testicular dysgenesis syndrome by altering the oxidative balance and SIRT1/PGC1α levels, and taxifolin is an ideal phytomedicine to prevent phthalate induced testicular dysgenesis syndrome.
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•DEHP induces testicular dysgenesis syndrome after in utero exposure in rats.•DEHP reduces testosterone biosynthesis after in utero exposure in rats.•DEHP induces abnormal aggregation of fetal Leydig cells.•DEHP reduces SIRT1/PGC1α levels and formation of multinucleated gonocytes.•Taxifolin rescues the DEHP-induced testicular dysgenesis syndrome an antioxidant.
This paper presents a novel learning method for precise eye localization, a challenge to be solved in order to improve the performance of face processing algorithms. Few existing approaches can ...directly detect and localize eyes with arbitrary angels in predicted eye regions, face images, and original portraits at the same time. To preserve rotation invariant property throughout the entire eye localization framework, a codebook of invariant local features is proposed for the representation of eye patterns. A heat map is then generated by integrating a 2-class sparse representation classifier with a pyramid-like detecting and locating strategy to fulfill the task of discriminative classification and precise localization. Furthermore, a series of prior information is adopted to improve the localization precision and accuracy. Experimental results on three different databases show that our method is capable of effectively locating eyes in arbitrary rotation situations (360° in plane).
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