Supplementing minerals beyond dietary requirements can increase the risk of toxicity and mineral excretion, making the selection of more bioavailable sources crucial. Thus, this work aimed to use ...metalloproteomics tools to investigate possible alterations in the hepatic proteome of broilers fed with diets containing two sources (sulfate and hydroxychloride) and two levels of copper (15 and 150 ppm) and manganese (80 and 120 ppm), totaling four treatments: low Cu/Mn SO
, high Cu/Mn SO
, low Cu/Mn (OH)Cl and high Cu/Mn (OH)Cl. The difference in abundance of protein spots and copper and manganese concentrations in liver and protein pellets were analyzed by analysis of variance with significance level of 5%. The Cu and Mn concentrations determined in liver and protein pellets suggested greater bioavailability of hydroxychloride sources. We identified 19 Cu-associated proteins spots, 10 Mn-associated protein spots, and 5 Cu and/or Mn-associated protein spots simultaneously. The analysis also indicated the induction of heat shock proteins and detoxification proteins in broilers fed with high levels of copper and manganese, suggesting the involvement of these proteins in metal tolerance and stress.
Diets for feedlot cattle must be a higher energy density, entailing high fermentable carbohydrate content. Feed additives are needed to reduce possible metabolic disorders. This study aimed to ...analyze the post-rumen effects of different levels of starch (25%, 35%, and 45%) and additives (monensin or a blend of essential oils and exogenous α-amylase) in diets for Nellore feedlot cattle. The cecum tissue proteome was analyzed via two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and then differentially expressed protein spots were identified with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The use of blends of essential oils associated with α-amylase as a feed additive promoted the upregulation of enzymes such as triosephosphate isomerase, phosphoglycerate mutase, alpha-enolase, beta-enolase, fructose-bisphosphate aldolase, pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), L-lactate dehydrogenase B, L-lactate dehydrogenase A chain, L-lactate dehydrogenase, and ATP synthase subunit beta, which promote the degradation of carbohydrates in the glycolysis and gluconeogenesis pathways and oxidative phosphorylation, support pyruvate metabolism through the synthesis of lactate from pyruvate, and participate in the electron transport chain, producing ATP from ADP in the presence of a proton gradient across the membrane. The absence of proteins related to inflammation processes (leukocyte elastase inhibitors) in the cecum tissues of animals fed essential oils and amylase may be because feed enzymes can remain active in the intestine and aid in the digestion of nutrients that escape rumen fermentation; conversely, the effect of monensin is more evident in the rumen and less than 10% results in post-ruminal action, corroborating the hypothesis that ionophore antibiotics have a limited effect on the microbiota and intestinal fermentation of ruminants. However, the increase in starch in these diets promoted a downregulation of enzymes linked to carbohydrate degradation, probably caused by damage to the cecum epithelium due to increased responses linked to inflammatory injuries.
The aim of the present study was to evaluate the differential expression of plasma proteins in broiler chickens supplemented with different sources (sulfates and hydroxychlorides) and levels of ...copper (15 and 150 mg kg
) and manganese (80 and 120 mg kg
). For this, plasma samples from 40 broiler chickens were used, divided into four experimental groups: S15-80 (15 ppm CuSO
and 80 ppm MnSO
), S150-120 (150 ppm CuSO
and 120 ppm MnSO
), H15-80 (15 ppm Cu(OH)Cl and 80 ppm Mn(OH)Cl), and H150-120 (150 ppm Cu(OH)Cl and 120 ppm Mn(OH)Cl). From plasma samples obtained from each bird from the same treatment, four pools were made considering 10 birds per group. Plasma proteome fractionation was performed by 2D-PAGE. Concentrations of the studied minerals were also evaluated in both plasma and protein pellet samples. A higher concentration of Cu and Mn was observed in the plasma and protein pellets of groups that received higher mineral supplementation levels compared to those receiving lower levels. Mn concentrations were higher in plasma and protein pellets of the hydroxychloride-supplemented groups than the sulfate-supplemented groups. Analysis of the gels revealed a total of 40 differentially expressed spots among the four treatments. Supplementation with different sources of minerals, particularly at higher levels, resulted in changes in protein regulation, suggesting a potential imbalance in homeostasis.
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The aim of this study was to characterize the proteins differentially expressed in the pectoralis major muscle of broilers supplemented with passion fruit seed oil (PFSO) under cyclic ...heat stress conditions. Ninety one-day-old male chicks were housed in cages arranged in a climatic chamber, where they were kept under cyclic heat stress for eight hours a day from the beginning to the end of the experiment. The birds were divided into two experimental groups, one group supplemented with 0.9% PFSO and a control group (CON) without PFSO supplementation. At 36 days of age, 18 birds were slaughtered to collect muscle samples. From pools of breast fillet samples from each group, proteolytic cleavage of the protein extracts was performed, and later, the peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The 0.9% PFSO supplementation revealed the modulation of 57 proteins in the pectoralis major muscle of broilers exposed to cyclic heat stress. Among them, four proteins were upregulated, and 46 proteins were downregulated. In addition, seven proteins were expressed only in the CON group. These results suggest that PFSO may increase heat tolerance, with a possible reduction in oxidative stress, activation of neuroprotective mechanisms, protection against apoptosis, decrease in inflammatory responses, and regulation of energy metabolism.
This study aimed to evaluate different energy sources in multiple supplements on performance, intake, and digestibility of Santa Ines sheep grazing urochloa grass (
Urochloa mosambicensis
) during ...the rainy season. The experimental area was divided into seven paddocks of 4 ha each, with an average of dry matter (DM) availability of 3.21 tn/ha. A completely randomized design was carried out, in which there were four treatments, and each treatment was repeated six times. Twenty-four intact lambs (average: 32.0 kg of body weight) were supplemented with a mineral mixture, the control group (MM), mesquite pod meal (MPM), wheat bran (WB), or sorghum grain (SG) as energy sources. The digestibility of DM and crude protein (CP) in MPM and WB is higher than that in MM and SG groups. Neutral detergent fiber (NDF) digestibility was similar between supplemented lambs, and it was higher than the MM. The supplementation promoted higher weight gain than in the control group (0.126 vs. 0.061 g/day, respectively;
P
< 0.001). The supplementation increased the DM, and CP intake. The NDF intake only increased in the WB group. The CP digestibility was higher for the MPM and WB groups than that for MM and SG ones (
P
< 0.001). Sheep supplementation in the rainy season increased the average daily gain (ADG). Any supplement tested in the present study can be used during the rainy season. The choice for the supplement will depend on the availability and costs of the mesquite pod meal, sorghum grain, or wheat bran.
This trial aimed to analyze the effects of including three byproducts from the biodiesel industry on the intake, digestibility, milk yield and composition, and feeding behavior of lactating cows. ...Eight crossbred Holstein-Zebu lactating cows with average body weight 525 ± 18.5 kg and average milk yield of 8 ± 1.45 kg day
–1
were assigned to four treatments (diets) in a double-Latin square design, as follows: a diet based on corn- and soybean meal-based concentrate and three diets with 20% inclusion of byproducts from the biodiesel industry (cottonseed cake, sunflower meal, and castor bean cake) on a total dry matter basis. The cows were housed in individual covered stalls with concrete floor equipped with individual concrete troughs for feeding and automatic drinkers, and fed diets containing 60% sugarcane and 40% concentrate. The inclusion of the byproducts in the diet changed the intake, digestibility of some nutritional components, milk yield and composition, and feeding behavior of lactating cows. The use of cottonseed cake and sunflower meal in the diet increased milk yield, and fat-corrected milk yield; while the use of castor bean cake reduced the intake, digestibility of dry matter and total digestible nutrients, milk yield, and fat-corrected milk yield. The inclusion of byproducts from the biodiesel industry in the diets did not change the fat, lactose, total solids, and solids-not-fat of milk. Therefore, the cottonseed cake and sunflower meal can be included at up to 20% of the total diet.
Inclusion of licuri meal in the diet of pasture dairy cows Costa, Evely Giovanna Leite; da Silva, Fabiano Ferreira; Silva, Robério Rodrigues ...
Tropical animal health and production,
11/2019, Letnik:
51, Številka:
8
Journal Article
Recenzirano
The objective of this study was to evaluate the inclusion of increasing levels of licuri meal as a substitute for soybean bran in the diet of pasture-based dairy cows and its effects on the intake, ...nutrient digestibility, and milk production and composition. Eight crossbred Holstein/Zebu cows were used in two 4 × 4 Latin square plots. The diets contained increasing percentage of licuri meal (0.0, 4.16, 7.09, and 9.45%) in the total diet, replacing soybean bran. The inclusion of licuri meal in the diet affected total dry matter intake and dry matter intake in relation to body weight, which presented a quadratic effect. The same behavior was observed for the consumption of EE, CP, TDN, and NDFap. The NFC consumption showed a linear decrease with the addition of the by-product in the diet. There was no influence of the inclusion of the licuri meal on the digestibility of the DM, CP, NFC, and TDN, while the digestibility of the NDFap and EE presented increasing linear effect. There was a reduction of milk production and food efficiency with the inclusion of increasing levels of the meal. The fat content in milk showed a linear increasing effect. Ditto for cholesterol. It is recommended to include not more than 7.00% of licuri meal for lactating cows with an average production of 20 kg day
−1
.
Dental classification of carcasses is used as a parameter of cattle maturity at slaughter, and it can influence carcass and meat quality traits. Brazilian beef-packing companies use the number of ...permanent incisor (PI) teeth as a parameter for bonus and certification of carcasses with superior quality. However, when non-castrated male such as F1 Angus-Nellore (Bos taurus×Bos indicus) are slaughtered, only animals without PI teeth are subsidized by the breed association. We evaluated these animals finished in feedlot for 180 days with zero versus two PI teeth on the carcass and meat quality traits. At the time of slaughter, 88 carcasses were selected, forming two treatments according to dental carcass maturity (0 versus 2 PI teeth; 44 animals per category). It was demonstrated that the number of PI teeth (0 versus 2 PI) did not influence (p>0.05) carcass (weights, yield, cooling loss, ribeye area and the backfat thickness) and meat quality traits (Longissimus thoracis chemical composition, color, cooking losses, shear force and pH). Thus, dental carcass maturity (zero versus two PI teeth) does not influence non-castrated male F1 Angus-Nellore finished in feedlot for 180 days. This is the first study to demonstrate that carcasses of non-castrated male F1 Angus-Nellore with two PI teeth should be subsidized in a similar way to those with zero PI teeth. Moreover, Brazilian beef-packing companies could produce heavier and leaner carcasses of acceptable quality though the use of crossbred cattle such as non-castrated F1 Angus Nellore.
Dental classification of carcasses is used as a parameter of cattle maturity at slaughter, and it can influence carcass and meat quality traits. Brazilian beef-packing companies use the number of ...permanent incisor (PI) teeth as a parameter for bonus and certification of carcasses with superior quality. However, when non-castrated male such as F1 Angus-Nellore (
×
) are slaughtered, only animals without PI teeth are subsidized by the breed association. We evaluated these animals finished in feedlot for 180 days with zero versus two PI teeth on the carcass and meat quality traits. At the time of slaughter, 88 carcasses were selected, forming two treatments according to dental carcass maturity (0 versus 2 PI teeth; 44 animals per category). It was demonstrated that the number of PI teeth (0 versus 2 PI) did not influence (p>0.05) carcass (weights, yield, cooling loss, ribeye area and the backfat thickness) and meat quality traits (
chemical composition, color, cooking losses, shear force and pH). Thus, dental carcass maturity (zero versus two PI teeth) does not influence non-castrated male F1 Angus-Nellore finished in feedlot for 180 days. This is the first study to demonstrate that carcasses of non-castrated male F1 Angus-Nellore with two PI teeth should be subsidized in a similar way to those with zero PI teeth. Moreover, Brazilian beef-packing companies could produce heavier and leaner carcasses of acceptable quality though the use of crossbred cattle such as non-castrated F1 Angus Nellore.
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