To understand the experience, training, and needs of midwives in their approach to perinatal grief. A descriptive cross‐sectional study was carried out using an online questionnaire with 26 questions ...related to institutional management and individual clinical practices in the care of a perinatal loss was developed by a team of midwives from the Hospital “La Mancha‐Centro” of Alcazar de San Juan (Ciudad Real). Strobe checklist was followed. A total of 267 midwives participated. A total of 92.1% (246) of the centers had specific protocols for action, but each professional applied their own criteria. The presence of a perinatal psychology team was nonexistent according to 88% (235) of those surveyed. Regarding their training and professional experience, 16.5% (44) of the midwives had never received training. Only 4.1% (11) of the midwives felt very prepared to care for women with a perinatal loss. Among the factors associated with greater application of recommended practices in the face of perinatal death by midwives were being a woman, having prior training on care during perinatal death, and a greater perception of preparation (p < 0.05). The perception of lack of preparation on the part of midwives in the accompaniment of these families was high.
In this work, an innovative green strategy has been developed for the analysis of twenty-seven endocrine disruptors, including bisphenols, alkylphenols and alkylphenol ethoxylates, phthalic acid ...esters and one adipate in tropical beverages. For this purpose, nine natural hydrophobic deep eutectic solvents based on the terpenoids camphor, thymol and menthol at different molar ratios were investigated for the first time as extractants for the liquid-liquid microextraction of the target analytes from coconut waters and Aloe Vera drinks. A mixture of camphor:thymol at molar ratio 1:2 (n/n) was selected as extraction solvent. Determination of the target analytes was carried out by ultra-high performance liquid chromatography coupled to tandem mass spectrometry. After optimisation of the determination and extraction conditions, the methodology was validated achieving good results in terms of linearity, as well as recovery values in the range 75–111% and limits of quantification from 0.137 to 10.08 μg/L. Finally, the developed methodology was applied to the analysis of commercially available samples, finding the presence of diethyl phthalate.
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•Camphor-based NAHDESs were tested as extraction solvents of xenobiotic contaminants.•Camphor:thymol (1:2, n/n) was applied for the extraction of BPs, APs, APnEOs and PAEs.•Plastic-related EDCs were analysed in coconut waters and Aloe Vera drinks.•The separation and quantification of target analytes were carried out by UHPLC-MS/MS.•The recovery and LOQs of the method were in the range 75–111% and 0.137–10.08 μg/L.
In its classical form, autophagy is an essential, homeostatic process by which cytoplasmic components are degraded in a double-membrane-bound autophagosome in response to starvation. Paradoxically, ...although autophagy is primarily a protective process for the cell, it can also play a role in cell death. The roles of autophagy bridge both the innate and adaptive immune systems and autophagic dysfunction is associated with inflammation, infection, neurodegeneration and cancer. In this review, we discuss the contribution of autophagy to inflammatory, infectious and neurodegenerative diseases, as well as cancer.
•Autophagy is a homeostatic process by which cell components are degraded.•Autophagy can also play a role in cell death.•Autophagy contributes to host defense and inflammation.•Autophagic dysfunction is associated with cancer.
Brain ischaemia (stroke) triggers an intense inflammatory response predominately mediated by the accumulation of inflammatory cells and mediators in the ischaemic brain. In this context, regulatory T ...(Treg) cells, a subpopulation of CD4+ T cells with immunosuppressive and anti‐inflammatory properties, are activated in the late stages of the disease. To date, the potential therapeutic usefulness of Treg cells has not been tested. In this study, we aimed to investigate whether Treg cells exert protection/repair following stroke. Both the adoptive transfer of Treg cells into ischaemic rats and the stimulation of endogenous T‐cell proliferation using a CD28 superagonist reduced the infarct size at 3–28 days following the ischaemic insult. Moreover, T cell‐treated animals had higher levels of FoxP3 and lower levels of IL‐1β, CD11b+ and CD68+ cells in the infarcted hemisphere when compared with control animals. However, T‐cell treatment did not alter the rate of proliferation of NeuN‐, NCAM‐ or CD31‐positive cells, thereby ruling out neurogenesis and angiogenesis in protection. These results suggest that adoptive transfer of T cells is a promising therapeutic strategy against the neurological consequences of stroke.
Dietary habits strongly influence gut microbiota. The aim of this study was to compare and correlated the abundance of Firmicutes and Bacteroidetes phyla, some representative bacteria of these phyla ...such as Bacteroides thetaiotaomicron, Prevotella, Faecalibacterium prausnitzii, Clostridium leptum and Bifidobacterium longum as a member of Actinobacteria phylum in young adults with their food intake. Faecal samples used came from lean subjects (BMI = 19.83 ± 0.94 kg/m
2
), overweight (BMI = 27.17 ± 0.51 kg/m
2
) and obese (BMI = 41.33 ± 5.25 kg/m
2
). There were significant differences in total studied gut microbiota between the overweight and lean groups. Members of the Firmicutes phylum, and Bifidobacterium longum, were more abundant in the lean group. The results suggest that diet rich in unsaturated fatty acids and fibre promote an abundant population of beneficial bacteria such as B. longum and Bacteroidetes. However, it has been considered that the results may be biased due to the size of the individuals studied; therefore the results could be only valid for the studied population.
Introduction: Bilateral vestibulopathy is an important cause of imbalance that is misdiagnosed. The clinical management of patients with bilateral vestibular loss remains difficult as there is no ...clear evidence for an effective treatment. In this paper, we try to analyze the effect of chronic electrical stimulation and adaptation to electrical stimulation of the vestibular system in humans when stimulating the otolith organ with a constant pulse train to mitigate imbalance due to bilateral vestibular dysfunction (BVD). Methods: We included 2 patients in our study with BVD according to Criteria Consensus of the Classification Committee of the Bárány Society. Both cases were implanted by using a full-band straight electrode to stimulate the otoliths organs and simultaneously for the cochlear stimulation we use a perimodiolar electrode. Results: In both cases Vestibular and clinical test (video head impulse test, videonistagmography cervical vestibular evoked myogenic potentials, cVEMP and oVEMP), subjective visual vertical test, computerized dynamic posturography, dynamic gait index, Time UP and Go test and dizziness handicap index) were performed. Posture and gait metrics reveal important improvement if compare with preoperartive situation. Oscillopsia, unsteadiness, independence and quality of life improved to almost normal situation. Discussion/Conclusion: Prosthetic implantation of the otolith organ in humans is technically feasible. Electrical stimulation might have potential effects on balance and this is stable after 1 year follow-up. This research provides new possibilities for the development of vestibular implants to improve gravito-inertial acceleration sensation, in this case by the otoliths stimulation.
The Uplink Wavefront Corrector System (UWCS) is a pathfinder instrument to demonstrate the uplink correction by Adaptive Optics techniques; this novel application can be directly usable in two ...fields: Free-Space Optical Communications and the generation of Laser Guide Stars. A Rayleigh LGS is propagated to the sky while the atmospheric wavefront aberrations are measured by a Shack-Hartmann WFS with 12 x 12 sub-apertures using a Natural Guide Star as a reference. The laser upwards propagation path is then pre-compensated by a 97-actuator deformable mirror. A scoring camera is attached to the finder telescope, next to the main aperture, in order to show the overall result, which is assessed in terms of beam power concentration. Present paper described the design process of the UWCS and its integration and testing in the Optical Ground Station telescope, at Teide Observatory (Spain).
The transesterification of sunflower oil was carried out using three different zeolites (mordenite, beta and X). A methyl ester content of 95.1
wt% was obtained at 60
°C employing zeolite X. The high ...catalytic activity of zeolite X gives processes competitive advantages over conventional processes based on homogeneous catalysis at the same temperature.
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The aim of this study was to analyse the catalytic performance of several heterogeneous catalysts in the transesterification of sunflower oil with methanol. In order to characterize the different catalysts, nitrogen adsorption/desorption and CO
2 temperature programmed desorption were used. The transesterification of sunflower oil was carried out using three different zeolites: mordenite, beta and X, to determine the influence of the kind of zeolite on the methyl ester production. The influence of the metal incorporation technique was studied using both impregnation and ion-exchange methods. Also, the transesterification reaction was carried out using catalysts with different metal loading. Finally, zeolite X was agglomerated with a binder, sodium bentonite, to study how the presence of a binder could change the catalytic performance of the zeolite. A methyl ester content of 93.5 and 95.1
wt% was obtained at 60
°C employing zeolite X with or without sodium bentonite, respectively. All biodiesel synthesized were characterized using the standard UNE-EN 14214. A complete deactivation study was carried out in order to check the sodium leaching from the catalyst. The results supported the hypothesis of a homogeneous-like mechanism where the alkali methoxide species were leached out.
A series of binderless activated carbon monoliths (ACMs) have been prepared from petroleum pitch and using KOH as activating agent. Characterization shows that these activated carbons combine a large ...“apparent” surface area (up to
S
BET
∼
3000
m
2/g) together with a well-developed narrow micropore size distribution. Dynamic column adsorption experiments using different volatile organic compounds (VOCs), ethanol and benzene, show that these activated carbons prepared from mesophase-based materials exhibit a superior saturation capacity compared to conventional carbon materials. The total amount adsorbed reaches values as high as 18
g/100
g AC and 40
g/100
g AC, for ethanol and benzene, respectively. These are the best results reported in the literature. The total amount adsorbed for both molecules correlates with the total volume of narrow micropores, thus confirming the pore size specificity required for the adsorption of VOC molecules. Regeneration studies show that ethanol can be easily desorbed at room temperature by flowing clean air through the adsorbent whereas benzene requires a further heating for complete desorption/regeneration.
•The principles and applications of analyte protectants (APs) in GC analysis are reviewed for the first time.•Some mechanisms and theoretical calculations involving APs and active sites are proposed ...for the first time.•Use of APs in a modern GC–MS high efficiency ion source design demonstrates strong signal enhancements of nonpolar analytes for the first time.•Effective use of APs in GC-HRMS using an orbitrap is reported in the literature for the first time.
Despite nearly 80 years of advancements in gas chromatography (GC), indirect chemical matrix effects (MEs), known as the matrix-induced response enhancement effect, still occur to cause a high bias in the GC analysis of susceptible analytes, unless precautions are taken. Matrix-matched calibration is one common option used in GC to compensate for the MEs, but this approach is usually inconvenient, imprecise, and inefficient. Other options, such as the method of standard additions, surface deactivation techniques, chemical derivatizations, priming the GC, and/or use of internal standards, also have flaws in practice. When methods are accommodating, the use of analyte protectants (APs) can provide the best practical solution to not only overcome MEs, but also to maximize analyte signal by increasing chromatographic and detection efficiencies for the analytes. APs address the source of MEs in every injection by filling active sites in the GC inlet, column, and detector, particularly in GC–MS, rather than the analytes that would otherwise undergo degradation, peak tailing, and/or diminished response due to interactions with the active sites. The addition of an adequate amount of APs (e.g. sugar derivatives) to all calibration standards and final extracts alike often leads to lower detection limits, better accuracy, narrower peaks, and greater robustness than the other options to compensate for MEs in GC. This article consists of a critical review of the scientific literature, proposal of mechanisms and theory, and re-evaluation studies involving APs for the first time in GC-orbitrap and GC–MS/MS with a high-efficiency ion source design. The findings showed that 1 µg each of co-injected shikimic acid and sorbitol in the former case, and 1 µg shikimic acid alone in the latter case, led to high quality results in multi-residue analysis of pesticides and environmental contaminants.
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