Insecticide use continues as the main strategy to control Aedes aegypti, the vector of dengue, Zika, chikungunya, and yellow fever. In the city of Tapachula, Mexico, mosquito control programs ...switched from pyrethroids to organophosphates for outdoor spatial spraying in 2013. Additionally, the spraying scheme switched from total coverage to focused control, prioritizing areas with higher entomological-virological risk. Five years after this strategy had been implemented, we evaluated the status and variability of insecticide resistance among Ae. aegypti collected at 26 sites in Tapachula.
We determined the lethal concentrations at 50% of the tested populations (LC50) using a bottle bioassay, and then, we calculated the resistance ratio (RR) relative to the susceptible New Orleans strain. Permethrin and deltamethrin (pyrethroids), chlorpyrifos and malathion (organophosphates), and bendiocarb (carbamate) were tested. The frequencies of the substitutions V1016I and F1534C, which are in the voltage-gated sodium channel and confer knockdown-resistance (kdr) to pyrethroid insecticides, were calculated. Despite 5 years having passed since the removal of pyrethroids from the control programs, Ae. aegypti remained highly resistant to permethrin and deltamethrin (RR > 10-fold). In addition, following 5 years of chlorpyrifos use, mosquitoes at 15 of 26 sites showed moderate resistance to chlorpyrifos (5- to 10-fold), and the mosquitoes from one site were highly resistant. All sites had low resistance to malathion (< 5-fold). Resistance to bendiocarb was low at 19 sites, moderate at five, and high at two. Frequencies of the V1016I ranged from 0.16-0.71, while C1534 approached fixation at 23 sites (0.8-1). Resistance profiles and kdr allele frequencies varied across Tapachula. The variability was not associated with a spatial pattern at the scale of the sampling.
Mosquito populations respond to selection pressure at a focal scale in the field. Spatial variation across sites highlights the importance of testing multiple sites within geographical regions.
► We studied the effect of pH and Mg in the crystallization of amorphous CaCO3 (ACC). ► The study combined synchrotron-based scattering with electron microscopy. ► The pH-dependent C speciation and ...hydration strength of Mg2+ control ACC structure. ► This ACC structure governs the ACC dissolution rate and crystallization pathway.
The effects of pH and Mg on the crystallization of amorphous calcium carbonate (ACC) to vaterite and/or calcite were studied using a combination of in situ time resolved synchrotron-based techniques and electron microscopy. The experiments showed that Mg increased the stability of ACC and favoured the formation of calcite over vaterite. A neutral (∼7) starting pH during mixing promoted the transformation of ACC into calcite via a dissolution/reprecipitation mechanism. Conversely, when ACC formed in a solution that started with a high initial pH (∼11.5), the transformation to calcite occurred via metastable vaterite, which formed via a spherulitic growth mechanism. In a second stage this vaterite transformed to calcite via a surface-controlled dissolution and recrystallization mechanism. These crystallization pathways can be explained as a consequence of the pH-dependent composition, local structure, stability and dissolution rates of ACC.
•LCOE estimation for Si-based monofacial and bifacial modules worldwide.•Monofacial PV systems are more cost-effective at latitudes below 40° for low albedo.•Bifacial PV systems are more ...cost-effective at latitudes beyond 40°.•Bifacial PV systems generate up to 12% more energy at latitudes below 65°, and up to 71% above 65°.
The present work studies the features of photovoltaic systems (PV) formed either by monofacial or bifacial crystalline p-type Si-based solar modules. To determine which module technology would be more favorable worldwide, a total of 55 locations around the globe are analyzed considering their weather profiles, market situation, and module electrical performance for 1 MWp systems. Levelized cost of electricity (LCOE) of the system has been used to decide which technology is more cost-effective. This is obtained for installations which allow any module orientation (AMO) or vertical module orientation (VMO). The results reveal that for latitudes above 40°, bifacial AMO designs are in general more cost-effective than monofacial AMO systems. This tendency is reversed, however, for latitudes below 40° with low albedo values. Nevertheless, if the albedo value is kept to a minimum between 0.12 and 0.30 (depending on the location), bifacial AMO designs can become more cost-effective. When comparing monofacial AMO against bifacial VMO, the latter is more cost-effective only for locations close to the poles, i.e., latitudes higher than 65°. Nonetheless, bifacial VMO designs could even reach lower LCOE values than the ones from monofacial AMO systems at latitudes below 65° if the albedo value is maintained to a minimum between 0.29 and 0.57 (depending on the location). This work will be useful not only to the scientific community, but also to PV installation companies to properly select the most adequate technology, and to optimize the installation design for particular projects.
First-line therapy for advanced non-small-cell lung cancer (NSCLC) that lacks targetable mutations is platinum-based chemotherapy. Among patients with a tumor proportion score for programmed death ...ligand 1 (PD-L1) of 50% or greater, pembrolizumab has replaced cytotoxic chemotherapy as the first-line treatment of choice. The addition of pembrolizumab to chemotherapy resulted in significantly higher rates of response and longer progression-free survival than chemotherapy alone in a phase 2 trial.
In this double-blind, phase 3 trial, we randomly assigned (in a 2:1 ratio) 616 patients with metastatic nonsquamous NSCLC without sensitizing EGFR or ALK mutations who had received no previous treatment for metastatic disease to receive pemetrexed and a platinum-based drug plus either 200 mg of pembrolizumab or placebo every 3 weeks for 4 cycles, followed by pembrolizumab or placebo for up to a total of 35 cycles plus pemetrexed maintenance therapy. Crossover to pembrolizumab monotherapy was permitted among the patients in the placebo-combination group who had verified disease progression. The primary end points were overall survival and progression-free survival, as assessed by blinded, independent central radiologic review.
After a median follow-up of 10.5 months, the estimated rate of overall survival at 12 months was 69.2% (95% confidence interval CI, 64.1 to 73.8) in the pembrolizumab-combination group versus 49.4% (95% CI, 42.1 to 56.2) in the placebo-combination group (hazard ratio for death, 0.49; 95% CI, 0.38 to 0.64; P<0.001). Improvement in overall survival was seen across all PD-L1 categories that were evaluated. Median progression-free survival was 8.8 months (95% CI, 7.6 to 9.2) in the pembrolizumab-combination group and 4.9 months (95% CI, 4.7 to 5.5) in the placebo-combination group (hazard ratio for disease progression or death, 0.52; 95% CI, 0.43 to 0.64; P<0.001). Adverse events of grade 3 or higher occurred in 67.2% of the patients in the pembrolizumab-combination group and in 65.8% of those in the placebo-combination group.
In patients with previously untreated metastatic nonsquamous NSCLC without EGFR or ALK mutations, the addition of pembrolizumab to standard chemotherapy of pemetrexed and a platinum-based drug resulted in significantly longer overall survival and progression-free survival than chemotherapy alone. (Funded by Merck; KEYNOTE-189 ClinicalTrials.gov number, NCT02578680 .).
Tip-enhanced Raman spectroscopy (TERS) has been rapidly improved over the past decade and opened up opportunities to study phonon properties of materials at the nanometer scale. In this Letter, we ...report on TERS of an ultrathin MoS2 flake on a nanostructured Au on silicon surface forming a two-dimensional (2D) crystal/plasmonic heterostructure. Au nanostructures (shaped in triangles) are prepared by nanosphere lithography, and then MoS2 is mechanically exfoliated on top of them. The TERS spectra acquired under resonance conditions at 638 nm excitation wavelength evidence strain changes spatially localized to regions as small as 25 nm in TERS imaging. We observe the highest Raman intensity enhancement for MoS2 on top of Au nanotriangles due to the strong electromagnetic confinement between the tip and a single triangle. Our results enable us to determine the local strain in MoS2 induced during heterostructure formation. The maximum frequency shift of E2g mode is determined to be (4.2 ± 0.8) cm–1, corresponding to 1.4% of biaxial strain induced in the MoS2 layer. We find that the regions of maximum local strain correspond to the regions of maximum topographic curvature as extracted from atomic force microscopy measurements. This tip-enhanced Raman spectroscopy study allows us to determine the built-in strain that arises when 2D materials interact with other nanostructures.
Expected increases in food demand and the need to limit the incorporation of new lands into agriculture to curtail emissions, highlight the urgency to bridge productivity gaps, increase farmers ...profits and manage risks in dryland cropping. A way to bridge those gaps is to identify optimum combination of genetics (G), and agronomic managements (M) i.e. crop designs (GxM), for the prevailing and expected growing environment (E). Our understanding of crop stress physiology indicates that in hindsight, those optimum crop designs should be known, while the main problem is to predict relevant attributes of the E, at the time of sowing, so that optimum GxM combinations could be informed. Here we test our capacity to inform that "hindsight", by linking a tested crop model (APSIM) with a skillful seasonal climate forecasting system, to answer "What is the value of the skill in seasonal climate forecasting, to inform crop designs?" Results showed that the GCM POAMA-2 was reliable and skillful, and that when linked with APSIM, optimum crop designs could be informed. We conclude that reliable and skillful GCMs that are easily interfaced with crop simulation models, can be used to inform optimum crop designs, increase farmers profits and reduce risks.
Quantum-mechanical shell effects are expected to strongly enhance nuclear binding on an "island of stability" of superheavy elements. The predicted center at proton number Z = 114, 120, or 126 and ...neutron number N = 184 has been substantiated by the recent synthesis of new elements up to Z = 118. However, the location of the center and the extension of the island of stability remain vague. High-precision mass spectrometry allows the direct measurement of nuclear binding energies and thus the determination of the strength of shell effects. Here, we present such measurements for nobelium and lawrencium isotopes, which also pin down the deformed shell gap at N = 152.
Laminar heterostructures composed by materials with different ferroic properties have been proposed for the control of magnetism with an applied voltage. We present here a study of the structural and ...magnetic properties of chemically disordered FePt ferromagnetic films of different thicknesses that have been sputter-deposited on ferroelectric PMN-PT (011) single crystals.
By means of static and dynamic magnetic measurements we have found that it is possible to switch the magnetization easy axis by 90° if an electric field is applied in a direction perpendicular to the film plane. Using ferromagnetic resonance techniques we have estimated the strain induced magnetoelectric coupling of the FePt/PMN-PT heterostructure, obtaining ME=477(15) Oe m/MV for FePt thicknesses greater than 26 nm, which corresponds to an average magnetoelastic constant B=−13.2(1.1) MJ/m3. The associated saturation magnetostriction was estimated in λs∼90 ppm. Films thinner than 26 nm present corresponding values that are smaller by approximately 20%. A possible explanation for this difference is the well known transition from planar to stripe-like magnetic domains that occurs in FePt around this film thickness. However, this thickness dependence of ME is relatively smaller than that found in other ferromagnets such as Fe or FeGa, which makes FePt a potential candidate for thin film applications in magnetoelectronic devices.
•Magnetoelectricity of FePt films deposited on ferroelectric PMN-PT (011) crystals.•Dependence of the magnetoelastic constant B of FePt alloy films on film thickness.•B changes when stripe domains are formed for thicknesses larger than ∼ 26 nm.
ZIKV is a new addition to the arboviruses circulating in the New World, with more than 1 million cases since its introduction in 2015. A growing number of studies have reported vector competence (VC) ...of Aedes mosquitoes from several areas of the world for ZIKV transmission. Some studies have used New World mosquitoes from disparate regions and concluded that these have a variable but relatively low competence for the Asian lineage of ZIKV.
Ten Aedes aegypti (L) and three Ae. albopictus (Skuse) collections made in 2016 from throughout Mexico were analyzed for ZIKV (PRVABC59-Asian lineage) VC. Mexican Ae. aegypti had high rates of midgut infection (MIR), dissemination (DIR) and salivary gland infection (SGIR) but low to moderate transmission rates (TR). It is unclear whether this low TR was due to heritable salivary gland escape barriers or to underestimating the amount of virus in saliva due to the loss of virus during filtering and random losses on surfaces when working with small volumes. VC varied among collections, geographic regions and whether the collection was made north or south of the Neovolcanic axis (NVA). The four rates were consistently lower in northeastern Mexico, highest in collections along the Pacific coast and intermediate in the Yucatan. All rates were lowest north of the NVA. It was difficult to assess VC in Ae. albopictus because rates varied depending upon the number of generations in the laboratory.
Mexican Ae. aegypti and Ae. albopictus are competent vectors of ZIKV. There is however large variance in vector competence among geographic sites and regions. At 14 days post infection, TR varied from 8-51% in Ae. aegypti and from 2-26% in Ae. albopictus.
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