Candida auris is an emerging multidrug-resistant fungus that causes a wide range of symptoms. We report finding 17 cases of C. auris infection that were originally misclassified but correctly ...identified 27.5 days later on average. Patients with a delayed diagnosis of C. auris had a 30-day mortality rate of 35.2%.
Case Series Study of Melioidosis, Colombia Rodríguez, José Y; Morales-López, Soraya E; Rodríguez, Gerson J ...
Emerging infectious diseases,
08/2019, Letnik:
25, Številka:
8
Journal Article
Recenzirano
Odprti dostop
We report 7 cases of melioidosis in Colombia and comparision of 4 commercial systems for identifying Burkholderia pseudomallei. Phoenix systems were not a definitive method for identifying B. ...pseudomallei. For accurate identification, we recommend including this bacterium in the library databases of matrix-assisted laser desorption/ionization mass spectrometry systems in Latin America.
Bacteremia by non-O1/non-O139 Vibrio cholerae is a rare entity associated with high mortality rates. We report a case of non-O1/non-O139 V. cholerae bacteremia confirmed by polymerase chain reaction ...and agglutination tests. The clinicoepidemiological characteristics and therapeutic options for this infection are also described.
•This is the first reported case of mucormycosis following esthetic surgery and was associated with medical tourism.•Mucormycosis should be considered in the differential diagnosis of necrotizing ...infections of the skin and soft tissue that evolve rapidly after cosmetic surgery performed in tropical or subtropical countries.•Elective cosmetic procedures are the most common medical tourism surgeries, and the most frequent complication is infection of the surgical site.
Mucormycosis caused by Saksenaea erythrospora is rarely reported in humans. Three previous cases have been reported in the literature, two associated with trauma (a sailing accident in Argentina and a combat trauma in Iraq) and one as a cause of invasive rhinosinusitis (India), all in immunocompetent patients . The first case of mucormycosis following esthetic surgery, associated with medical tourism, is reported herein.
A case study of an S. erythrospora infection in an immunocompetent woman after the completion of esthetic surgery (dermolipectomy and breast augmentation) is reported. The infection presented as a rapidly progressive necrotizing infection of the skin and soft tissue, which required a bilateral mastectomy and extensive surgical debridement associated with prolonged antifungal therapy. The organism was identified phenotypically and confirmed biologically after rDNA amplification and sequencing. Two months later, the patient remains hospitalized awaiting the start of reconstructive surgeries. The present case is, to the best of the authors’ knowledge, the first report from Colombia.
Mucormycosis should be considered in the differential diagnosis of necrotizing infections of the skin and soft tissue that evolve rapidly after cosmetic surgery performed in tropical or subtropical countries.
has become a major health threat due to its transmissibility, multidrug resistance and severe outcomes. In a case-control design, 74 hospitalised patients with candidemia were enrolled. In total, 22 ...cases (29.7%) and 52 controls (
, 21.6%;
, 21.6%;
, 21.6%;
, 1.4%) were included and analysed in this study. Risk factors, clinical and microbiological characteristics and outcomes of patients with
and non-
species (NACS) candidemia were compared. Previous fluconazole exposure was significantly higher in
candidemia patients (OR 3.3; 1.15-9.5). Most
isolates were resistant to fluconazole (86.3%) and amphotericin B (59%) whilst NACS isolates were generally susceptible. No isolates resistant to echinocandins were detected. The average time to start antifungal therapy was 3.6 days. Sixty-three (85.1%) patients received adequate antifungal therapy, without significant differences between the two groups. The crude mortality at 30 and 90 days of candidemia was up to 37.8% and 40.5%, respectively. However, there was no difference in mortality both at 30 and 90 days between the group with candidemia by
(31.8%) and by NACS (42.3%) (OR 0.6; 95% IC 0.24-1.97) and 36.4% and 42.3% (0.77; 0.27-2.1), respectively. In this study, mortality due to candidemia between
and NACS was similar. Appropriate antifungal therapy in both groups may have contributed to finding no differences in outcomes.
is an emerging pathogen in the Americas. Cases of mother-to-child transmission of
are rare and probably occur by placental or perinatal infection. We report the first case of native gestational and ...neonatal melioidosis in the Western hemisphere. The isolated strains in the mother and newborn were confirmed by whole-genome sequencing and identified as a novel sequence type ST1748. The comparison of both genomes revealed a nucleotide similarity of 100%. Melioidosis should be considered within the differential diagnosis of febrile illness or pneumonia in pregnant women and newborns from endemic areas of the Americas.
Background Neuronal stem cells (NSCs) are promising for neurointestinal disease therapy. Although NSCs have been isolated from intestinal musclularis, their presence in mucosa has not been well ...described. Mucosa‐derived NSCs are accessible endoscopically and could be used autologously. Brain‐derived Nestin‐positive NSCs are important in endogenous repair and plasticity. The aim was to isolate and characterize mucosa‐derived NSCs, determine their relationship to Nestin‐expressing cells and to demonstrate their capacity to produce neuroglial networks in vitro and in vivo.
Methods Neurospheres were generated from periventricular brain, colonic muscularis (Musc), and mucosa–submucosa (MSM) of mice expressing green fluorescent protein (GFP) controlled by the Nestin promoter (Nestin‐GFP). Neuronal stem cells were also grown as adherent colonies from intestinal mucosal organoids. Their differentiation potential was assessed using immunohistochemistry using glial and neuronal markers. Brain and gut‐derived neurospheres were transplanted into explants of chick embryonic aneural hindgut to determine their fate.
Key Results Musc‐ and MSM‐derived neurospheres expressed Nestin and gave rise to cells of neuronal, glial, and mesenchymal lineage. Although Nestin expression in tissue was mostly limited to glia co‐labelled with glial fibrillary acid protein (GFAP), neurosphere‐derived neurons and glia both expressed Nestin in vitro, suggesting that Nestin+/GFAP+ glial cells may give rise to new neurons. Moreover, following transplantation into aneural colon, brain‐ and gut‐derived NSCs were able to differentiate into neurons.
Conclusions & Inferences Nestin‐expressing intestinal NSCs cells give rise to neurospheres, differentiate into neuronal, glial, and mesenchymal lineages in vitro, generate neurons in vivo and can be isolated from mucosa. Further studies are needed for exploring their potential for treating neuropathies.
Lingual tuberculosis in an HIV/AIDS Patient Rodríguez, José Y; Rodríguez, Gerson J; Álvarez-Moreno, Carlos A
International journal of infectious diseases,
05/2017, Letnik:
58, Številka:
C
Journal Article
Recenzirano
Odprti dostop
Highlights • Oral Tb should be included in the differential diagnosis of oral lesions, irrespective of the existence of pulmonary signs and symptoms. • The diagnosis of oral Tb is difficult and can ...be performed by pathological examination and PCR assays.
•The thick blood smear is a useful, rapid, and low cost technique for the diagnosis of disseminated histoplasmosis.•Thick blood smears by Field stain method allow a high concentration of leukocytes ...per field to be visualized.
Paracoccidioidomycosis simulating Crohn’s disease Rodríguez, José Y.; Rodríguez, Gerson J.; Álvarez-Moreno, Carlos A. ...
International journal of infectious diseases,
07/2018, Letnik:
72
Journal Article
Recenzirano
Odprti dostop
•Paracoccidioidomycosis should be considered in the differential diagnosis of colonic lesions that mimic Crohn’s disease in patients from endemic countries, before the start of immunosuppressive ...therapy.•Molecular diagnostic methods such as panfungal PCR can be useful in the diagnosis of invasive fungal infections.•Serological techniques may be a good option paracoccidioidomicosis diagnosis, however their specificity and sensitivity vary depending on the type of antigen used.
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