Current diagnostic standards involve severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection in nasopharyngeal swabs (NPS), but saliva is an attractive and noninvasive option for ...diagnosis. The objectives were to determine the performance of saliva in comparison with NPS for detecting SARS‐CoV‐2 and to compare the optimized home brew reverse‐transcription polymerase chain reaction (RT‐PCR) with a commercial RT‐PCR. Paired NPS and saliva specimens were prospectively collected and tested by RT‐PCR from patients presenting at an emergency room with signs and symptoms compatible with coronavirus disease‐2019. A total of 348 samples from 174 patients were tested by RT‐PCR assays. Among 174 patients with symptoms, 63 (36%) were SARS‐CoV‐2 positive in NPS using the optimized home‐brew PCR. Of these 63 patients, 61 (98%) were also positive in saliva. An additional positive SARS‐CoV‐2 saliva was detected in a patient with pneumonia. Kappa Cohen's coefficient agreement between NPS and saliva was 0.96 (95% confidence interval CI, 0.90–0.99). Median Ct values in NPS versus saliva were 18.88 (interquartile range IQR, 15.60–23.58; range, 11.97–38.10) versus 26.10 (IQR, 22.75–30.06; range, 13.78–39.22), respectively (p < .0001). The optimized home‐brew RT‐PCR demonstrated higher analytical and clinical sensitivity compared with the commercial RT‐PCR assay. A high sensitivity (98%) and agreement (kappa 0.96) in saliva samples compared to NPS was demonstrated when using an optimized home‐brew PCR even when the viral load in saliva was lower than in NPS. This noninvasive sample is easy to collect, requires less consumable and avoids discomfort to patients. Importantly, self‐collection of saliva can diminish exposure to healthcare personnel.
The reflectance of the Earth is a fundamental climate parameter that we measured from Big Bear Solar Observatory between 1998 and 2017 by observing the earthshine using modern photometric techniques ...to precisely determine daily, monthly, seasonal, yearly and decadal changes in terrestrial albedo from earthshine. We find the inter‐annual fluctuations in albedo to be global, while the large variations in albedo within individual nights and seasonal wanderings tend to average out over each year. We measure a gradual, but climatologically significant ∼0.5 W/m2 decline in the global albedo over the two decades of data. We found no correlation between the changes in the terrestrial albedo and measures of solar activity. The inter‐annual pattern of earthshine fluctuations are in good agreement with those measured by CERES (data began in 2001) even though the satellite observations are sensitive to retroflected light while earthshine is sensitive to wide‐angle reflectivity. The CERES decline is about twice that of earthshine.
Plain Language Summary
The net sunlight reaching the Earth's climate system depends on the solar irradiance and the Earth's reflectance (albedo). We have observed earthshine from Big Bear Solar Observatory to measure the terrestrial albedo. For earthshine we measure the sunlight reflected from Earth to the dark part of the lunar face and back to the nighttime observer, yielding an instantaneous large‐scale reflectance of the Earth. In these relative measurements, we also observe the sunlit, bright part of the lunar face. We report here reflectance data (monthly, seasonal and annual) covering two decades, 1998–2017. The albedo shows a decline corresponding to a net climate forcing of about 0.5 W/m2. We find no correlation between measures of solar cycle variations and the albedo variations. The first precise satellite measures of terrestrial albedo came with CERES. CERES global albedo data (2001‐) show a decrease in forcing that is about twice that of earthshine measurements. The evolutionary changes in albedo motivate continuing earthshine observations as a complement to absolute satellite measurements, especially since earthshine and CERES measurements are sensitive to distinctly different parts of the angular reflectivity. The recent drop in albedo is attributed to a warming of the eastern pacific, which is measured to reduce low‐lying cloud cover and, thereby, the albedo.
Key Points
We report on two decades of earthshine measurements of the earth's reflectance made from Big Bear Solar Observatory yielding a large‐scale terrestrial albedo
We find a decline in albedo between 1998 and 2017, corresponding to a radiative increase of 0.5 W/m2, which is climatologically significant
The CERES data show the same behavior, which is attributed to a reversal of the Pacific Decadal Oscillation reducing the Earth's albedo
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) is a master regulator of glycolysis by its ability to synthesize fructose-2,6-bisphosphate, a potent allosteric activator of ...6-phosphofructo-1-kinase. Being a substrate of the E3 ubiquitin ligase anaphase-promoting complex-Cdh1 (APC(Cdh1)), PFKFB3 is targeted to proteasomal degradation in neurons. Here, we show that activation of N-methyl-D-aspartate subtype of glutamate receptors (NMDAR) stabilized PFKFB3 protein in cortical neurons. Expressed PFKFB3 was found to be mainly localized in the nucleus, where it is subjected to degradation; however, expression of PFKFB3 lacking the APC(Cdh1)-targeting KEN motif, or following NMDAR stimulation, promoted accumulation of PFKFB3 and its release from the nucleus to the cytosol through an excess Cdh1-inhibitable process. NMDAR-mediated increase in PFKFB3 yielded neurons having a higher glycolysis and lower pentose-phosphate pathway (PPP); this led to oxidative stress and apoptotic neuronal death that was counteracted by overexpressing glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the PPP. Furthermore, expression of the mutant form of PFKFB3 lacking the KEN motif was sufficient to trigger oxidative stress and apoptotic death of neurons. These results reveal that, by inhibition of APC(Cdh1), glutamate receptors activation stabilizes PFKFB3 thus switching neuronal metabolism leading to oxidative damage and neurodegeneration.
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•Digestate was valorised through solid-state fermentation into biopesticides.•Bacillus thuringiensis can grow on digestate to produce biopesticides.•Reactors of different type ...(insulated and mixed) and scale (10–100 L) were used.•Sporulation over 80% is obtained in all the studied reactors.•Stirred non-insulated reactor is pointed as the best option for further scale-up.
The main goal of this work is the production of a biopesticide through solid-state fermentation of biowaste digestate inoculated with Bacillus thuringiensis (Bt) at pilot scale using different configurations of reactors. Fermentations were carried out using insulated and non-insulated, stirred and non-stirred reactors at different scales (10, 22 and 100 L) in order to assess the influence of the reactor configuration on the biopesticide production process. A maximum temperature of 60 °C was reached in 10-L insulated non-stirred reactors where increments of Bt viable cells and spores with respect to initial values of 1.9 and 171.6 respectively, were attained. In contrast, when temperature was regulated by using 22-L non-insulated stirred reactors the increment of viable cells and spores were 0.8 and 1.9, respectively, at a stable temperature of 27 °C. When the non-insulated stirred reactor was scaled up to 100-L, the increase of viable cells and spore counts were 1.2 and 3.8 respectively, with an average temperature of 28 °C. These results demonstrated that the election of a proper reactor configuration is important when considering the development of a new SSF process, especially when dealing with non-conventional substrates as digestate.
Quasar accretion disc winds observed via broad absorption lines (BALs) in the UV produce strong continuous absorption in X-rays. The X-ray absorber is believed to serve critically as a radiative ...shield to keep the outflow ionizations low enough for radiative driving. However, previous studies have shown that 'mini-BAL' and narrow absorption line (NAL) outflows have dramatically less X-ray absorption than BALs. Here, we examine X-ray and rest-frame UV spectra of eight mini-BAL quasars with outflow speeds in the range 0.1-0.2c to test the hypothesis that these extreme speeds require a strong shield. We find that the X-ray absorption is weak or moderate, with neutral-equivalent column densities N
H < few × 1022 cm−2, consistent with mini-BALs at lower speeds. We use photoionization models to show that the amount of shielding consistent with our data is too weak to control the outflow ionizations and, therefore, it is not important for the acceleration. Shielding in complex geometries also seems unlikely because the alleged shield would need to extinguish the ionizing far-UV flux while avoiding detection in X-rays and the near-UV. We argue that the outflow ionizations are kept moderate, instead, by high gas densities in small clouds. If the mini-BALs form at radial distances of the order of R ∼ 2 pc from the central quasar (broadly consistent with theoretical models and with the mini-BAL variabilities observed here and in previous work), and the total column densities in the mini-BAL gas are N
H 1021 cm−2, then the total radial extent of outflow clouds is only ΔR
clouds 3 × 1013 cm in cases of no/weak shielding or ΔR
clouds 3 × 1014 cm behind the maximum shield allowed by our data. This implies radial filling factors ΔR
clouds/R 5 × 10− 6 or 5 × 10− 5 for the unshielded or maximally shielded cases, respectively. Compared to the transverse sizes 8 × 1015 cm (based on measured line depths), the outflows have shapes like thin 'pancakes' viewed face-on, or they occupy larger volumes like a spray of many dense clouds with a small volume filling factor. These results favour models with magnetic confinement in magnetic disc winds. To the extent that BALs, mini-BALs and NALs probe the same general outflow phenomenon, our result for dense substructures should apply to all three outflow types.
We present a 3D map of extinction in the northern Galactic plane derived using photometry from the INT/WFC Photometric Hα Survey of the northern Galactic plane. The map has fine angular ( ∼ 10 ...arcmin) and distance (100 pc) sampling allied to a significant depth (≳5 kpc). We construct the map using a method based on a hierarchical Bayesian model described in a previous article by Sale. In addition to mean extinction, we also measure differential extinction, which arises from the fractal nature of the interstellar medium, and show that it will be the dominant source of uncertainty in estimates of extinction to some arbitrary position. The method applied also furnishes us with photometric estimates of the distance, extinction, effective temperature, surface gravity, and mass for ∼38 million stars. Both the extinction map and the catalogue of stellar parameters are made publicly available via http://www.iphas.org/extinction.
The adult pineal gland is composed of pinealocytes, astrocytes, microglia, and other interstitial cells that have been described in detail. However, factors that contribute to pineal development have ...not been fully elucidated, nor have pineal cell lineages been well characterized. We applied systematic double, triple and quadruple labeling of cell-specific markers on prenatal, postnatal and mature rat pineal gland tissue combined with confocal microscopy to provide a comprehensive view of the cellular dynamics and cell lineages that contribute to pineal gland development. The pineal gland begins as an evagination of neuroepithelium in the roof of the third ventricle. The pineal primordium initially consists of radially aligned Pax6+ precursor cells that express vimentin and divide at the ventricular lumen. After the tubular neuroepithelium fuses, the distribution of Pax6+ cells transitions to include rosette-like structures and later, dispersed cells. In the developing gland all dividing cells express Pax6, indicating that Pax6+ precursor cells generate pinealocytes and some interstitial cells. The density of Pax6+ cells decreases across pineal development as a result of cellular differentiation and microglial phagocytosis, but Pax6+ cells remain in the adult gland as a distinct population. Microglial colonization begins after pineal recess formation. Microglial phagocytosis of Pax6+ cells is not common at early stages but increases as microglia colonize the gland. In the postnatal gland microglia affiliate with Tuj1+ nerve fibers, IB4+ blood vessels, and Pax6+ cells. We demonstrate that microglia engulf Pax6+ cells, nerve fibers, and blood vessel-related elements, but not pinealocytes. We conclude that microglia play a role in pineal gland formation and homeostasis by regulating the precursor cell population, remodeling blood vessels and pruning sympathetic nerve fibers.
This paper proposes a novel control for voltage-source inverters with the capability to flexibly operate in grid-connected and islanded modes. The control scheme is based on the droop method, which ...uses some estimated grid parameters such as the voltage and frequency and the magnitude and angle of the grid impedance. Hence, the inverter is able to inject independently active and reactive power to the grid. The controller provides a proper dynamics decoupled from the grid-impedance magnitude and phase. The system is also able to control active and reactive power flows independently for a large range of impedance grid values. Simulation and experimental results are provided in order to show the feasibility of the control proposed.
Massive phytoplankton proliferation, and the consequent release of toxic metabolites, can be responsible for seafood poisoning outbreaks: filter-feeding mollusks, such as shellfish, mussels, oysters ...or clams, can accumulate these toxins throughout the food chain and present a threat for consumers' health. Particular environmental and climatic conditions favor this natural phenomenon, called harmful algal blooms (HABs); the phytoplankton species mostly involved in these toxic events are dinoflagellates or diatoms belonging to the genera
,
,
, and
. Substantial economic losses ensue after HABs occurrence: the sectors mainly affected include commercial fisheries, tourism, recreational activities, and public health monitoring and management. A wide range of symptoms, from digestive to nervous, are associated to human intoxication by biotoxins, characterizing different and specific syndromes, called paralytic shellfish poisoning, amnesic shellfish poisoning, diarrhetic shellfish poisoning, and neurotoxic shellfish poisoning. This review provides a complete and updated survey of phycotoxins usually found in marine invertebrate organisms and their relevant properties, gathering information about the origin, the species where they were found, as well as their mechanism of action and main effects on humans.
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