A gram-negative, facultatively anaerobic bacterium with appendages was isolated from continuous cultures with a seawater-sediment suspension containing hexadecane as the sole carbon source. Although ...this organism was isolated from a hexadecane-degrading bacterial community, it was not able to degrade hexadecane. However, this bacterium was able to use different sugars and amino acids for growth, indicating that it probably profits from the lysis or from products like surfactants of other cells in the community. 16S rDNA analysis demonstrated that the isolated strain is phylogenetically related to the family Flavobacteriaceae of the phylum 'Cytophaga-Flavobacterium-Bacteroides'. Evidence based on phenotypic characteristics and 16S rDNA analysis supports the conclusion that this bacterium is distinct from its nearest relative, Zobellia uliginosa (90.72% similarity in 16S rRNA gene sequence), and from the other genera of the Flavobacteriaceae. It is therefore proposed that the isolated marine bacterium represents a novel taxon, designated Muricauda ruestringensis gen. nov., sp. nov. The type strain is strain B1T (= DSM 13258T = LMG 19739T).
Many studies have found that discounting is hyperbolic rather than constant. Hyperbolic discounting induces time-inconsistent behavior and is becoming increasingly popular in economic applications. ...Most studies that provide evidence in favor of hyperbolic discounting either are merely qualitative or they depend on assumptions about, or parametric fittings of, utility functions. This paper provides a quantitative measure for the degree of deviation from stationarity and the induced time-inconsistency that can overcome the problems mentioned. This measure, the hyperbolic factor, also provides simple preference foundations of the most popular discount functions. Moreover, it can easily be calculated from data and does not require knowledge of utility. Thus, the hyperbolic factor provides an easy tool for theoretical preference foundations, critical empirical tests, and quantitative measurements of hyperbolic discounting.
•Cross-flow causes a peak in the stream-wise root mean square at the gap centre.•The stream-wise length of the coherent structures increases at low Reynolds.•The gap vortex streets are closer to the ...gap centreline for high Reynolds.
The enhancement of heat transfer from fuel rods to coolant of a Liquid Metal Fast Reactor (LMFR) decreases the fuel temperature and, thus, improves the safety margin of the reactor. One of the mechanisms that increases heat transfer consists of large coherent structures that can occur across the gap between adjacent rods. This work investigates the flow between two curved surfaces, representing the gap between two adjacent fuel rods. The aim is to investigate the presence of the aforementioned structures and to provide, as partners in the EU SESAME project, an experimental benchmark for numerical validation to reproduce the thermal hydraulics of Gen-IV LMFRs. The work investigates also the applicability of Fluorinated Ethylene Propylene (FEP) as Refractive Index Matching (RIM) material for optical measurements.
The experiments are conducted on two half-rods of 15 mm diameter opposing each other inside a Perspex box with Laser Doppler Anemometry (LDA). Different channel Reynolds numbers between Re = 600 and Re = 30,000 are considered for each P/D (pitch-to-diameter ratio).
For high Re, the stream wise velocity root mean square vrms between the two half rods is higher near the walls, similar to common channel flow. As Re decreases, however, an additional central peak in vrms appears at the gap centre, away from the walls. The peak becomes clearer at lower P/D ratios and it also occurs at higher flow rates. Periodical behaviour of the span wise velocity across the gap is revealed by the frequency spectrum and the frequency varies with P/D and decreases with Re. The study of the stream wise velocity component reveals that the structures become longer with decreasing Re. As Re increases, these structures are carried along the flow closer to the gap centre, whereas at low flow rates they are spread over a wider region. This becomes even clearer with smaller gaps.
To investigate the longitudinal performance of a surgically implanted neuroprosthesis for lower-extremity exercise, standing, and transfers after spinal cord injury.
Case series.
Research or ...outpatient physical therapy departments of 4 academic hospitals.
Subjects (N=15) with thoracic or low cervical level spinal cord injuries who had received the 8-channel neuroprosthesis for exercise and standing.
After completing rehabilitation with the device, the subjects were discharged to unrestricted home use of the system. A series of assessments were performed before discharge and at a follow-up appointment approximately 1 year later.
Neuroprosthesis usage, maximum standing time, body weight support, knee strength, knee fatigue index, electrode stability, and component survivability.
Levels of maximum standing time, body weight support, knee strength, and knee fatigue index were not statistically different from discharge to follow-up (P>.05). Additionally, neuroprosthesis usage was consistent with subjects choosing to use the system on approximately half of the days during each monitoring period. Although the number of hours using the neuroprosthesis remained constant, subjects shifted their usage to more functional standing versus more maintenance exercise, suggesting that the subjects incorporated the neuroprosthesis into their lives. Safety and reliability of the system were demonstrated by electrode stability and a high component survivability rate (>90%).
This group of 15 subjects is the largest cohort of implanted lower-extremity neuroprosthetic exercise and standing system users. The safety and efficiency data from this group, and acceptance of the neuroprosthesis as demonstrated by continued usage, indicate that future efforts toward commercialization of a similar device may be warranted.
The objective of this study was to (1) qualitatively identify the perceived determinants of physical activity among women who have experienced severe preeclampsia, and (2) examine whether these ...determinants are consistent with the overarching processes outlined in the integrated behavior change (IBC) model, a novel model that describes physical activity as being a result of motivational, volitional, and automatic processes.
Patients (n = 35) of the Follow-Up PreEClampsia (FUPEC) Outpatient Clinic, Erasmus MC, the Netherlands, participated in an anonymous online survey. The main outcomes under study were their perceived determinants of physical activity. Responses were analyzed using thematic analysis.
Thirteen themes emerged from the analysis. Six themes corresponded with motivational processes (future health, perceived ability, attitude, future reward or regret, physical appearance, and doing it for others), two with volitional processes (scheduling and planning), and two with automatic processes (affect and stress). Three themes were classified as environmental factors (time constraint, social support, and physical environment).
A range of facilitating and hindering factors were described by women with prior severe preeclampsia as the determinants of their physical activity. These factors corresponded well with the overarching motivational, volitional, and automatic processes described in the IBC model. In addition, motivational and environmental factors beyond the IBC model were described. Addressing these perceived determinants could enhance the efficacy of physical activity interventions in this population.
Motivational, volitional, automatic, and environmental factors drive physical activity in women with prior severe preeclampsia.
Molecular profiling of tumors shows that triple-negative breast cancer (TNBC) can be stratified into mesenchymal (claudin-low breast cancer; CLBC) and epithelial subtypes (basal-like breast cancer; ...BLBC). Subtypes differ in underlying genetics and in response to therapeutics. Several reports indicate that therapeutic strategies that induce lipid peroxidation or proteotoxicity may be particularly effective for various cancers with a mesenchymal phenotype such as CLBC, for which no specific treatment regimens exist and outcomes are poor. We hypothesized that silver nanoparticles (AgNPs) can induce proteotoxic stress and cause lipid peroxidation to a greater extent in CLBC than in BLBC. We found that AgNPs were lethal to CLBCs at doses that had little effect on BLBCs and were non-toxic to normal breast epithelial cells. Analysis of mRNA profiles indicated that sensitivity to AgNPs correlated with expression of multiple CLBC-associated genes. There was no correlation between sensitivity to AgNPs and sensitivity to silver cations, uptake of AgNPs, or proliferation rate, indicating that there are other molecular factors driving sensitivity to AgNPs. Mechanistically, we found that the differences in sensitivity of CLBC and BLBC cells to AgNPs were driven by peroxidation of lipids, protein oxidation and aggregation, and subsequent proteotoxic stress and apoptotic signaling, which were induced in AgNP-treated CLBC cells, but not in BLBC cells. This study shows AgNPs are a specific treatment for CLBC and indicates that stratification of TNBC subtypes may lead to improved outcomes for other therapeutics with similar mechanisms of action.
The association between red blood cell (RBC) transfusion strategies and health care-associated infection is not fully understood.
To evaluate whether RBC transfusion thresholds are associated with ...the risk of infection and whether risk is independent of leukocyte reduction.
MEDLINE, EMBASE, Web of Science Core Collection, Cochrane Central Register of Controlled Trials, Cochrane Database of Sytematic Reviews, ClinicalTrials.gov, International Clinical Trials Registry, and the International Standard Randomized Controlled Trial Number register were searched through January 22, 2014.
Randomized clinical trials with restrictive vs liberal RBC transfusion strategies.
Twenty-one randomized trials with 8735 patients met eligibility criteria, of which 18 trials (n = 7593 patients) contained sufficient information for meta-analyses. DerSimonian and Laird random-effects models were used to report pooled risk ratios. Absolute risks of infection were calculated using the profile likelihood random-effects method.
Incidence of health care-associated infection such as pneumonia, mediastinitis, wound infection, and sepsis.
The pooled risk of all serious infections was 11.8% (95% CI, 7.0%-16.7%) in the restrictive group and 16.9% (95% CI, 8.9%-25.4%) in the liberal group. The risk ratio (RR) for the association between transfusion strategies and serious infection was 0.82 (95% CI, 0.72-0.95) with little heterogeneity (I2 = 0%; τ2 <.0001). The number needed to treat (NNT) with restrictive strategies to prevent serious infection was 38 (95% CI, 24-122). The risk of infection remained reduced with a restrictive strategy, even with leukocyte reduction (RR, 0.80 95% CI, 0.67-0.95). For trials with a restrictive hemoglobin threshold of <7.0 g/dL, the RR was 0.82 (95% CI, 0.70-0.97) with NNT of 20 (95% CI, 12-133). With stratification by patient type, the RR was 0.70 (95% CI, 0.54-0.91) in patients undergoing orthopedic surgery and 0.51 (95% CI, 0.28-0.95) in patients presenting with sepsis. There were no significant differences in the incidence of infection by RBC threshold for patients with cardiac disease, the critically ill, those with acute upper gastrointestinal bleeding, or for infants with low birth weight.
Among hospitalized patients, a restrictive RBC transfusion strategy was associated with a reduced risk of health care-associated infection compared with a liberal transfusion strategy. Implementing restrictive strategies may have the potential to lower the incidence of health care-associated infection.
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