Glioblastoma is the most frequent brain tumor in adults and is the most lethal form of human cancer. Despite the improvements in treatments, survival of patients remains poor. In order to identify ...microRNAs (miRs) involved in glioma tumorigenesis, we evaluated, by a miRarray, differential expression of miRs in the tumorigenic glioma LN-18, LN-229 and U87MG cells compared with the non-tumorigenic T98G cells. Among different miRs we focused our attention on miR-221 and -222. We demonstrated the presence of a binding site for these two miRs in the 3' untranslated region of the protein tyrosine phosphatase μ (PTPμ). Previous studies indicated that PTPμ suppresses cell migration and is downregulated in glioblastoma. Significantly, we found that miR-221 and -222 overexpression induced a downregulation of PTPμ as analyzed by both western blot and real-time PCR. Furthermore, miR-222 and -221 induced an increase in cell migration and growth in soft agar in glioma cells. Interestingly, the re-expression of PTPμ gene was able to revert the miR-222 and -221 effects on cell migration. Furthermore, we found an inverse correlation between miR-221 and -222 and PTPμ in human glioma cancer samples. In conclusion, our results suggest that miR-221 and -222 regulate glioma tumorigenesis at least in part through the control of PTPμ protein expression.
Flows of angular rock fragments are released down a concave upward chute in the laboratory to study their mobility. This mobility is measured as the reciprocal of the apparent coefficient of friction ...that is equal to the vertical drop of the center of mass of the granular material divided by its horizontal distance of travel. Our experiments show that the finer the grain size (with all the other features the same), the larger the mobility of the center of mass. We believe this to be due to the fact that in finer grain size flows there are less agitated particles per unit of flow mass, so that these flows dissipate less energy per unit of travel distance. Our experiments show also that the larger the volume (with all the other features the same), the larger the apparent coefficient of friction. We believe this to be so because the frontal portion of a flow reaches the less steep part of a curved slope and stops before the rear portion, preventing the rear portion and the center of mass from traveling farther downhill. This phenomenon (which is more prominent in larger‐volume flows, whose rear and frontal ends are more distant) counteracts the decrease of energy dissipation per unit of flow mass because of the decrease of particle agitation per unit of flow mass that is expected when the volume of a flow increases (with all the other features the same). Our analysis generates a functional relationship between the dimensionless apparent coefficient of friction and a scaling parameter whose numerator is equal to the mean grain size multiplied by the cube root of the deposit volume and whose denominator is the square of the channel width. The purpose of our experiments is to understand the dynamics of rock avalanches and dense pyroclastic flows.
Key Points
Finer grain size flows have smaller apparent coefficients of friction
Larger‐volume flows have larger apparent coefficients of friction
Larger‐volume deposits have more distal frontal end
In this study, 139 Tuscan Wastewater Treatment Plants (WWTPs) were analyzed with the aim of evaluating their efficiency and highlighting the main efficiency drivers, as well as distinguishing among ...wastewater features, WWTP technology, other features of WWTPs, output variables, and sludge disposal. From a methodological point of view, the proposed method includes an ordinary least squares analysis of total plant costs regressed on a set of 28 exogenous variables and a two-stage Data Envelopment Analysis model, where efficiency scores are obtained through weight restrictions. Moreover, the results of this study demonstrate that, with the exception of the “other features of WWTPs”, all other clusters of variables exert a negative effect on cost savings; in other words, larger scale of operations and higher usage of the productive capacity (grouped as “other features of WWTPs”) can improve cost efficiency.
Continuous monitoring of food properties during drying is of stark relevance for the food processing industry. The main task of this research is to apply laser backscattering technology to ...simultaneously predict variations in hardness, moisture content and soluble solids content (SSC) of apples during drying. A laser diode with a wavelength of 635
nm was used to non-destructively analyze photon migration in the apple tissue. The backscattering area in pixel numbers, representing the illuminated area after laser light injection, and light luminescence measured by grey values were used for estimating changes in internal quality parameters during drying. A decrease in moisture content caused a reduction in photon scattering determined by diminished light propagation in the fruit tissue. Additionally, scattering images showed less photon migration as the SSC increased due to the light absorption of light at 635
nm by fruit compounds. To estimate moisture content, calibration models gave the highest coefficients of determination (
R
2
=
0.8 and 0.89) with standard errors of cross validation (SECV) of 11.6 and 9.8 for backscattering area and light luminescence, respectively. However, predictions obtained for SSC were slightly lower. Laser light measurement at 635
nm was found to be adequate for predicting changes in moisture content and SSC of apple during drying over different stages. On the contrary, photon scattering at 635
nm is not recommended as estimator of change in hardness during apple drying, based on the results.
A typical feature of ageing is a chronic, low-grade inflammation characterized by a general increase in the production of pro-inflammatory cytokines and inflammatory markers ("inflamm-ageing"). This ...status may slowly damage one or several organs, especially when unfavorable genetic polymorphisms and epigenetic alterations are concomitant, leading to an increased risk of frailty together with the onset of age-related chronic diseases. The contribution of different tissues (adipose tissue, muscle), organs (brain, liver), immune system and ecosystems (gut microbiota) to age-related inflammation ("inflamm-ageing") will be discussed in this review in the context of its onset/progression leading to site-restricted and systemic effects. Moreover, some of the possible strategies and therapies to counteract the different sources of molecular mediators which lead to the age-related inflammatory phenotype will be presented.
The role of particles heavier than the fluid (glass spheres in water) in a turbulent open channel flow over a smooth bed is examined at volume concentration about $10^{-3}$. The present work focuses ...on the dynamical interaction between the solid (particles) and the fluid phases in the near-wall region. Experimental measurements have been performed by means of phase Doppler anemometry to acquire two velocity components, particle size and concentration data simultaneously; the Reynolds number of the flow was close to 15 000. It is observed that in the particle-laden flow, the vertical profiles of the streamwise mean velocity (for both fluid and solid phases) are reduced in the outer layer ($y^{ + }\,{ >}\, 20$), but increased in the viscous sublayer ($y^{ + }\,{<}\,5$) in comparison to the clear-water conditions, leading to an apparent slip kinematic boundary condition close to the wall ($y^{ + } \,{\approx}\,2$). Moreover, in the presence of solid particles, the flow exhibits a velocity close to the wall ($y^{ + }\,{ <}\, 15$) which is smaller than that of the particles, while in the outer layer the opposite takes place. In particle-laden flow, turbulence intensities of the streamwise and especially of the vertical velocity are damped for $y^{ + }\,{>}\,10$–20 (depending on particle inertia) but enhanced in the very near-wall region ($y^{ + }\,{ <}\, 5$), as is the Reynolds stress. These findings can be explained if they are referred to the mechanism of particle entrainment and deposition, which takes place close to the wall. This mechanism is related to particle inertia and to the dynamic of the structure of near-wall turbulence, which connects the buffer and outer regions with the very near-wall region. A significant momentum exchange between the two phases, which is particularly effective in the buffer region, is revealed by the quadrant analysis of the Reynolds stresses.
In the field of photodynamic therapy (PDT), optimization of the in vivo therapeutic efficacy needs a comprehensive study of the photo-killing action spectrum that depends on both the photosensitizer ...(PS) absorption and the tissue optical properties. This is especially true in the case of gastric infections by Helicobacter pylori: PS absorption has been largely investigated in vitro, while the contribution of tissue optical properties and illumination geometry has been poorly studied, despite being parameters that reflect the specific in vivo conditions. To investigate their influence, we focussed on the case of a point-like light source positioned in the antrum. This models a therapeutic device developed by our team which consists of a LED-based ingestible pill. By a simple 3D illumination model, our approach mediates light-tissue interaction over the illuminated stomach wall surface, then calculates its average transmittance T by means of a 1D model representative of the mean gastric mucosa structure. Finally, by merging T(λ) with the photosensitizers' absorption we obtained the in vivo action spectrum. This shows two peaks at about 500 and 630 nm, indicating a noticeable influence of the tissue with respect to in vitro studies, where the action spectrum reflects PS absorption only. Our approach defines one average action spectrum for this specific therapeutic context, which reflects the need to choose one emission spectrum for the light source used. The proposed methodology could be applied to any other illumination geometry of cave organs, provided appropriate model modifications for the light source and tissue characteristics are made.
Glioblastoma is the most frequent brain tumor in adults and is the most lethal form of human cancer. Despite the improvements in treatments, survival of patients remains poor. In order to identify ...microRNAs (miRs) involved in glioma tumorigenesis, we evaluated, by a miRarray, differential expression of miRs in the tumorigenic glioma LN-18, LN-229 and U87MG cells compared with the non-tumorigenic T98G cells. Among different miRs we focused our attention on miR-221 and -222. We demonstrated the presence of a binding site for these two miRs in the 3' untranslated region of the protein tyrosine phosphatase μ (PTPμ). Previous studies indicated that PTPμ suppresses cell migration and is downregulated in glioblastoma. Significantly, we found that miR-221 and -222 overexpression induced a downregulation of PTPμ as analyzed by both western blot and real-time PCR. Furthermore, miR-222 and -221 induced an increase in cell migration and growth in soft agar in glioma cells. Interestingly, the re-expression of PTPμ gene was able to revert the miR-222 and -221 effects on cell migration. Furthermore, we found an inverse correlation between miR-221 and -222 and PTPμ in human glioma cancer samples. In conclusion, our results suggest that miR-221 and -222 regulate glioma tumorigenesis at least in part through the control of PTPμ protein expression.
Efficient water management is a priority in the European Union, since the operational efficiency of many water utilities is very low compared to best practice. Several countries are restructuring the ...water industry to save costs. Larger-scale operations and vertical integration are promoted to achieve scale and scope economies; however, the literature is not unanimous that such economies exist. There is also little evidence of the effect of customer density on costs. This article offers some insights into this matter, analysing the Danish water industry by a two-stage Data Envelopment Analysis approach to investigate the effects of size, scope and density in the wastewater industry. The results show that the Danish wastewater industry is positively affected by vertical integration and higher population density: firms that serve more than 100 person per km of sewer and combine water and wastewater services achieve better efficiency. Size does not have any significant influence on global efficiency, although technical pure efficiency decreases statistically with firm size.
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