The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard ...diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n-6) to α-linolenic acid (ALA, C18:3n-3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3-10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB-Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets.
During the early stages of pregnancy, the uterine endometrium undergoes dramatic morphologic and functional changes accompanied with dynamic variation in gene expression. Pregnancy-stage specific ...differentially expressed gene (DEG)-transcript-probes were investigated and identified by comparing endometrium transcriptome at 9th day (9D), 12th day (12D) and 16th day (16D) of early pregnancy in Polish large-white (PLW) gilts. Endometrium comparisons between 9D-vs-12D, 9D-vs-16D and 12D-vs-16D of early pregnancy identified 6049, 374 and 6034 highly significant DEG-transcript-probes (
< 0.001; >2 FC). GO term enrichment analysis identified commonly shared upregulated endometrial DEG-transcript-probes (
< 0.001; >2 FC), that were regulating the gene functions of anatomic structure development and transport (
), DNA-binding and
activity (
), ion-binding and
activity (
), cell proliferation and apoptosis activity (
. Downregulated DEG-transcript-probes (
< 0.001; >2 FC) were involved in regulating the gene functions of
activity (
TC616413 gene-transcript and
LOC100525539. Moreover, blastn comparison of microarray-probes sequences against
assembly identified commonly shared upregulated endometrial DEG-transcript-probes (E < 0.06; >2 FC), that were regulating the gene functions of reproduction and growth (
), cytoskeleton organization and
activity (
),
activity (
), enzyme-binding and cell-population proliferation (VAV3), cancer-susceptibility candidate gene (
), cytoskeletal protein-binding (
), ion-binding, enzyme regulator activity (
) Downregulated endometrial DEG-transcript-probes (E < 0.06; >2FC) were involved in regulating the gene functions of signal-transduction (
catabolic and metabolic processes (
. Microarray validation experiment on selected candidate genes showed complementarity to significant endometrial DEG-transcript-probes responsible for the regulation of immune response (
), lipid metabolism (
), cell-adhesion (
),
(
), intercellular transmission (
), cell-adhesion (
) and response to stimuli (
) was confirmed by RT-PCR. This study provides a clue that identified pregnancy-stage specific microarray transcript probes could be considered as candidate genes for recognition and establishment of early pregnancy in the pig.
The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard ...diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n–6) to α-linolenic acid (ALA, C18:3n–3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3–10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB—Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets.
Urbański, P., Pierzchała, M., Terman, A., Kamyczek, M., Różycki, M., Roszczyk, A. and Czarnik, U. 2015. The relationship between the polymorphism of the porcine CAST gene and productive traits in ...pigs. Can. J. Anim. Sci. 95: 361–367. The aim of the study was to characterize the polymorphism of the calpastatin gene identified with ApaLI, Hpy188I and PvuII restriction enzymes in two pig breeds and one line bred in Poland, and to evaluate the relationship between the CAST genotype and carcass traits. The analysis covered a total of 617 pigs of two breeds, Polish Landrace (185) and Polish Large White (216), and synthetic line L990 (216). All animals studied appeared to be monomorphic at two loci: CAST/ApaLI and CAST/Hpy188I, while three genotypes were observed at CAST/PvuII locus. Statistical analysis was carried out for each breed separately using the least square methods of the GLM procedure. The model included the effect of the CAST genotype, fixed effect of the RYR1 genotype and the effect of the sire. Because the RYR1 genotype could significantly modify the effect of other genes, the effect of the RYR1 genotype was included in the statistical model. The relationship between the polymorphism and several productive traits was identified in each of the study groups of pigs. Animals carrying the heterozygous genotype at this locus showed most extreme values for some of the traits tested. Our results suggest that the CAST /PvuII genotype might be utilized in the selection of valuable pig carcass traits, particularly weight and size of the loin.
Cystatin B gene is a candidate gene for carcass and meat quality traits of pigs and belongs to the family 1 of cysteine proteinase inhibitors. The enzyme is a cathepsin inhibitor and the proteolytic ...cystatin/cathepsin system plays an important role in the growth and development of muscles. Investigations presented here covered 707 pigs from different genetic groups reared in Poland. The aim of this study was to characterise the polymorphism of the CSTB gene identified with restriction endonuclease: TaqI and PvuII, and to analyse the relation between the CSTB genotypes and carcass traits. All tested animals proved to be monomorphic at the CSTB/TaqI locus. All three possible genotypes were observed with regard to the second CSTB/PvuII locus. In Polish Large White and Polish Landrace pigs the highest frequency was reported for BB homozygotes. The association between CSTB and carcass traits was found only in Polish Landrace pigs for the meat content of carcass, meat content of valuable cuts and weight of the loin.
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