Novel chemistry for vector control is urgently needed to counter insecticide resistance in mosquitoes. Here a new meta-diamide insecticide, broflanilide (TENEBENALTM), was evaluated in East African ...experimental huts in Moshi, northern Tanzania. Two consecutive experimental hut trials with broflanilide 50WP were conducted; the first evaluating the efficacy of three concentrations, 50 mg/m2, 100 mg/m2, and 200 mg/m2 using a prototype formulation, and the second trial evaluating an improved formulation. The IRS treatments were applied on both mud and concrete surfaces and efficacy was monitored over time. The mortality, blood-feeding inhibition and exiting behaviour of free-flying wild mosquitoes was compared between treatment arms. Additionally, cone assays with pyrethroid-susceptible and resistant mosquito strains were conducted in the huts to determine residual efficacy. The first trial showed a dosage-mortality response of the prototype formulation and 3-8 months of residual activity, with longer activity on concrete than mud. The second trial with an improved formulation showed prolonged residual efficacy of the 100 mg/m2 concentration to 5-6 months on mud, and mosquito mortality on the concrete surface ranged between 94-100% for the full duration of the trial. In both trials, results with free-flying, wild Anopheles arabiensis echoed the mortality trend shown in cone assays, with the highest dose inducing the highest mortality and the improved formulation showing increased mortality rates. No blood-feeding inhibition or insecticide-induced exiting effects were observed with broflanilide. Broflanilide 50WP was effective against both susceptible and pyrethroid-resistant mosquito strains, demonstrating an absence of cross resistance between broflanilide and pyrethroids. The improved formulation, which has now been branded VECTRONTM T500, resulted in a prolonged residual efficacy. These results indicate the potential of this insecticide as an addition to the arsenal of IRS products needed to maintain both control of malaria and resistance management of malaria-transmitting mosquitoes.
On 14 November 2016, northeastern South Island of New Zealand was struck by a major moment magnitude (
) 7.8 earthquake. Field observations, in conjunction with interferometric synthetic aperture ...radar, Global Positioning System, and seismology data, reveal this to be one of the most complex earthquakes ever recorded. The rupture propagated northward for more than 170 kilometers along both mapped and unmapped faults before continuing offshore at the island's northeastern extent. Geodetic and field observations reveal surface ruptures along at least 12 major faults, including possible slip along the southern Hikurangi subduction interface; extensive uplift along much of the coastline; and widespread anelastic deformation, including the ~8-meter uplift of a fault-bounded block. This complex earthquake defies many conventional assumptions about the degree to which earthquake ruptures are controlled by fault segmentation and should motivate reevaluation of these issues in seismic hazard models.
Abstract
Flooding of low-lying Arctic regions has the potential to warm and thaw permafrost by changing the surface reflectance of solar insolation, increasing subsurface soil moisture, and ...increasing soil thermal conductivity. However, the impact of flooding on permafrost in the continuous permafrost environment remains poorly understood. To address this knowledge gap, we used a combination of available flooding data on the Ikpikpuk delta and a numerical model to simulate the hydro-thermal processes under coastal floodplain flooding. We first constructed the three most common flood events based on water level data on the Ikpikpuk: snowmelt floods in the late spring and early summer, middle and late summer floods, and floods throughout the whole spring and summer. Then the impact of these flooding events on the permafrost was simulated for one-dimensional permafrost columns using the Advanced Terrestrial Simulator (ATSv1.0), a fully coupled permafrost-hydrology and thermal dynamic model. Our results show that coastal floods have an important impact on coastal permafrost dynamics with a cooling effect on the surficial soil and a warming effect on the deeper soil. Cumulative flooding events over several years can cause continuous warming of the deep subsurface but cool down the surficial layer. Flood timing is a primary control of the vertical extent of the permafrost thaw and the active layer deepening.
Coupling to metal nanoparticles can increase the fluorescence intensity and photostability of fluorescent probes, and this plasmon-enhanced fluorescence is particularly promising for the dimmer ...fluorescent proteins common in biological imaging. Here, we measure the intensity distribution of single Cy3.5 dye molecules and mCherry fluorescent proteins one at a time as they adsorb on a conformal surface 4.8–61.0 nm thick over a gold nanorod (NR). The emission intensities for both types of fluorophores depend nonmonotonically on the spacer thickness, and an optimal spacer thickness of ∼10 nm is observed for both fluorophores using two different spacer layer materials. Emission from fluorophores coupled to metal nanoparticles is affected by two competing processes: an enhanced spontaneous decay rate and quenching via nonradiative antenna modes. After averaging over a conformal surface, the product of the simulated enhanced local electric field intensity and the quantum efficiency modification reproduces the experimental 10 nm ideal spacer thickness. Overall, up to a 3.4-fold average enhancement in fluorescence intensity was achieved despite the simple geometry, based on biocompatible, tunable, and economic colloidal gold NRs. This study of the distance dependence of single-molecule plasmon-enhanced fluorescence shows promise for super-resolving cellular membrane proteins naturally positioned above an extracellular substrate.
Coastal saltwater intrusion (SWI) is one key factor that affects the hydrology, ecology, and biogeochemistry of coastal ecosystems. Future climate change, especially intensified sea level rise (SLR), ...is expected to trigger SWI to encroach on coastal freshwater aquifers more intensively. Numerous studies have investigated decadal/century scale SWI under SLR by assuming a static coastal landscape topography. However, coastal landscapes are highly dynamic in response to SLR, and the impact of coastal landscape evolution on SWI has received very little attention. Thus, this study used a coastal marsh landscape as an example and investigated how coastal marsh evolution affects future SWI with a physically‐based coastal hydro‐eco‐geomorphologic model, Advanced Terrestrial Simulator. Our numerical experiments showed that it is very likely that the marsh elevation increases with future SLR due to sediment deposition, and a depression zone is formed due to different marsh accretion rates between the ocean boundary and the inland. We found that marsh accretion may significantly reduce the surface saltwater inflow at the ocean boundary, and the evolved topographic depression zone may prolong the residence time of surface ponded saltwater, affecting subsurface salinity distribution differently. We also predicted that marshlands may become more sensitive to upland freshwater supply under future SLR, compared with previous predictions without marsh evolution. This study demonstrates the importance of coastal evolution to coastal freshwater‐saltwater interaction. The eco‐geomorphologic effect may not be ignored when evaluating coastal SWI under SLR at decadal or century scales.
Key Points
The effect of coastal marsh evolution on future saltwater intrusion is examined for the first time
Marsh accretion under sea level rise may significantly reduce surface seawater inflow and prolong the surface seawater residence time
Future saltwater intrusion on the evolved marsh landscape may become more sensitive to upland groundwater inflows
Measuring the activity and temperature of rats is commonly required in biomedical research. Conventional approaches necessitate single housing, which affects their behavior and wellbeing. We have ...used a subcutaneous radiofrequency identification (RFID) transponder to measure ambulatory activity and temperature of individual rats when group-housed in conventional, rack-mounted home cages. The transponder location and temperature is detected by a matrix of antennae in a baseplate under the cage. An infrared high-definition camera acquires side-view video of the cage and also enables automated detection of vertical activity. Validation studies showed that baseplate-derived ambulatory activity correlated well with manual tracking and with side-view whole-cage video pixel movement. This technology enables individual behavioral and temperature data to be acquired continuously from group-housed rats in their familiar, home cage environment. We demonstrate its ability to reliably detect naturally occurring behavioral effects, extending beyond the capabilities of routine observational tests and conventional monitoring equipment. It has numerous potential applications including safety pharmacology, toxicology, circadian biology, disease models and drug discovery.
The 2016 moment magnitude (Mw) 7.8 Kaikoura earthquake was one of the largest ever to hit New Zealand. Hamling et al. show with a new slip model that it was an incredibly complex event. Unlike most ...earthquakes, multiple faults ruptured to generate the ground shaking. A remarkable 12 faults ruptured overall, with the rupture jumping between faults located up to 15 km away from each other. The earthquake should motivate rethinking of certain seismic hazard models, which do not presently allow for this unusual complex rupture pattern. Science, this issue p. eaam7194 On 14 November 2016 (local time), northeastern South Island of New Zealand was struck by a major moment magnitude (Mw) 7.8 earthquake. The Kaikoura earthquake was the most powerful experienced in the region in more than 150 years. The whole of New Zealand reported shaking, with widespread damage across much of northern South Island and in the capital city, Wellington. The earthquake straddled two distinct seismotectonic domains, breaking multiple faults in the contractional North Canterbury fault zone and the dominantly strike-slip Marlborough fault system. Earthquakes are conceptually thought to occur along a single fault. Although this is often the case, the need to account for multiple segment ruptures challenges seismic hazard assessments and potential maximum earthquake magnitudes. Field observations from many past earthquakes and numerical models suggest that a rupture will halt if it has to step over a distance as small as 5 km to continue on a different fault. The Kaikoura earthquake's complexity defies many conventional assumptions about the degree to which earthquake ruptures are controlled by fault segmentation and provides additional motivation to rethink these issues in seismic hazard models. Field observations, in conjunction with interferometric synthetic aperture radar (InSAR), Global Positioning System (GPS), and seismology data, reveal the Kaikoura earthquake to be one of the most complex earthquakes ever recorded with modern instrumental techniques. The rupture propagated northward for more than 170 km along both mapped and unmapped faults before continuing offshore at the island's northeastern extent. A tsunami of up to 3 m in height was detected at Kaikoura and at three other tide gauges along the east coast of both the North and South Islands. Geodetic and geological field observations reveal surface ruptures along at least 12 major crustal faults and extensive uplift along much of the coastline. Surface displacements measured by GPS and satellite radar data show horizontal offsets of ~6 m. In addition, a fault-bounded block (the Papatea block) was uplifted by up to 8 m and translated south by 4 to 5 m. Modeling suggests that some of the faults slipped by more than 20 m, at depths of 10 to 15 km, with surface slip of ~10 m consistent with field observations of offset roads and fences. Although we can explain most of the deformation by crustal faulting alone, global moment tensors show a larger thrust component, indicating that the earthquake also involved some slip along the southern end of the Hikurangi subduction interface, which lies ~20 km beneath Kaikoura. Including this as a fault source in the inversion suggests that up to 4 m of predominantly reverse slip may have occurred on the subduction zone beneath the crustal faults, contributing ~10 to 30% of the total moment. Although the unusual multifault rupture observed in the Kaikoura earthquake may be partly related to the geometrically complex nature of the faults in this region, this event emphasizes the importance of reevaluating how rupture scenarios are defined for seismic hazard models in plate boundary zones worldwide. (A and B ) Photos showing the coastal uplift of 2 to 3 m associated with the Papatea block labeled in (C). The inset in (A) shows an aerial view of New Zealand. Red lines denote the location of known active faults. The black box indicates the Marlborough fault system. (C ) Three-dimensional displacement field derived from satellite radar data. The vectors represent the horizontal displacements, and the colored background shows the vertical displacements. On 14 November 2016, northeastern South Island of New Zealand was struck by a major moment magnitude (Mw) 7.8 earthquake. Field observations, in conjunction with interferometric synthetic aperture radar, Global Positioning System, and seismology data, reveal this to be one of the most complex earthquakes ever recorded. The rupture propagated northward for more than 170 kilometers along both mapped and unmapped faults before continuing offshore at the island's northeastern extent. Geodetic and field observations reveal surface ruptures along at least 12 major faults, including possible slip along the southern Hikurangi subduction interface; extensive uplift along much of the coastline; and widespread anelastic deformation, including the ~8-meter uplift of a fault-bounded block. This complex earthquake defies many conventional assumptions about the degree to which earthquake ruptures are controlled by fault segmentation and should motivate reevaluation of these issues in seismic hazard models.
Objectives
To undertake a prospective multicentre national audit of penile prosthesis practice in the UK over a 3‐year period.
Patients and Methods
Data were submitted by urological surgeons as part ...of the British Association of Urological Surgeons Penile Prosthesis National Audit. Patients receiving a penile prosthesis (inflatable or malleable) were included as part of a prospective registry over a 3‐year period. Data were validated and then analysed using a software package (Tableau).
Results
A total of 1071 penile prosthesis procedures were included from 22 centres. The three commonest aetiological factors for erectile dysfunction were diabetes, prostate surgery and Peyronie's disease. Of the recorded data, inflatable penile prostheses were the commonest devices implanted, with 665 devices used (62.1%), whereas malleable prostheses accounted for 14.2% of the implants. Recorded intra‐operative complications included urethral injury (0.7%, n = 7), corporal perforation (1.1%, n = 12) and cross‐over (0.6%, n = 6). Known postoperative complications were recorded in 9.8% of patients (74/752), with the two most frequently reported being postoperative penile pain (n = 11) and scrotal haematoma (n = 14).
Conclusion
This baseline analysis is the largest prospective registry of penile prostheses procedures to date. The data show that, over the 3‐year collection period in the UK, there are now fewer surgeons performing the procedure, together with a reduction in the number of centres. Peri‐operative complications were infrequent, and the rate of implant abortion (e.g. as a result of urethral injury) was very low. Further follow‐up data will be required to publish long‐term outcomes and patient satisfaction.
By following single fluorescent molecules in a microscope, single-particle tracking (SPT) can measure diffusion and binding on the nanometer and millisecond scales. Still, although SPT can at its ...limits characterize the fastest biomolecules as they interact with subcellular environments, this measurement may require advanced illumination techniques such as stroboscopic illumination. Here, we address the challenge of measuring fast subcellular motion by instead analyzing single-molecule data with spatiotemporal image correlation spectroscopy (STICS) with a focus on measurements of confined motion. Our SPT and STICS analysis of simulations of the fast diffusion of confined molecules shows that image blur affects both STICS and SPT, and we find biased diffusion rate measurements for STICS analysis in the limits of fast diffusion and tight confinement due to fitting STICS correlation functions to a Gaussian approximation. However, we determine that with STICS, it is possible to correctly interpret the motion that blurs single-molecule images without advanced illumination techniques or fast cameras. In particular, we present a method to overcome the bias due to image blur by properly estimating the width of the correlation function by directly calculating the correlation function variance instead of using the typical Gaussian fitting procedure. Our simulation results are validated by applying the STICS method to experimental measurements of fast, confined motion: we measure the diffusion of cytosolic mMaple3 in living Escherichia coli cells at 25 frames/s under continuous illumination to illustrate the utility of STICS in an experimental parameter regime for which in-frame motion prevents SPT and tight confinement of fast diffusion precludes stroboscopic illumination. Overall, our application of STICS to freely diffusing cytosolic protein in small cells extends the utility of single-molecule experiments to the regime of fast confined diffusion without requiring advanced microscopy techniques.
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