Objectives
The COVID‐19 crisis is a significant stressor worldwide. The physical and emotional condition of individuals with pain sensitization syndromes who are experiencing the pandemic may worsen. ...This study investigated the contribution of life changes due to the coronavirus to emotional distress in individuals with a diagnosis of chronic central sensitization pain and tested whether the associations between level of pain and sensitization were independent of or mediated by emotional distress.
Methods
Spanish individuals with chronic pain (N = 362) completed an online survey on direct or indirect exposure to the consequences of COVID‐19, pain intensity, and emotional distress. They also completed central sensitization questionnaires.
Results
An association was found between changes in daily routines and pain intensity, emotional distress, and sensitization scores. Correlations were found between emotional distress, sensitization, and pain intensity. Significant predictors of emotional distress were age, difficulty in receiving medical care, changes in daily routines, and diminished social support. Emotional distress did not mediate the association between sensitization and pain intensity.
Conclusion
Due to the COVID‐19 situation, individuals with central sensitization pain syndromes may be at higher risk of developing psychological distress. Interdisciplinary interventions involving psychologists are urgently needed to provide this population with appropriate health care.
In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in ...plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has ten AGO genes of which AGO1, AGO2, and AGO7 have been shown to play roles in antiviral defense. A genetic analysis was used to identify and characterize the roles of AGO proteins in antiviral defense against Turnip mosaic virus (TuMV) in Arabidopsis. AGO1, AGO2 and AGO10 promoted anti-TuMV defense in a modular way in various organs, with AGO2 providing a prominent antiviral role in leaves. AGO5, AGO7 and AGO10 had minor effects in leaves. AGO1 and AGO10 had overlapping antiviral functions in inflorescence tissues after systemic movement of the virus, although the roles of AGO1 and AGO10 accounted for only a minor amount of the overall antiviral activity. By combining AGO protein immunoprecipitation with high-throughput sequencing of associated small RNAs, AGO2, AGO10, and to a lesser extent AGO1 were shown to associate with siRNAs derived from silencing suppressor (HC-Pro)-deficient TuMV-AS9, but not with siRNAs derived from wild-type TuMV. Co-immunoprecipitation and small RNA sequencing revealed that viral siRNAs broadly associated with wild-type HC-Pro during TuMV infection. These results support the hypothesis that suppression of antiviral silencing during TuMV infection, at least in part, occurs through sequestration of virus-derived siRNAs away from antiviral AGO proteins by HC-Pro. These findings indicate that distinct AGO proteins function as antiviral modules, and provide a molecular explanation for the silencing suppressor activity of HC-Pro.
Abstract
We present 888 visual-wavelength spectra of 122 nearby type II supernovae (SNe II) obtained between 1986 and 2009, and ranging between 3 and 363 days post-explosion. In this first paper, we ...outline our observations and data reduction techniques, together with a characterization based on the spectral diversity of SNe II. A statistical analysis of the spectral matching technique is discussed as an alternative to nondetection constraints for estimating SN explosion epochs. The time evolution of spectral lines is presented and analyzed in terms of how this differs for SNe of different photometric, spectral, and environmental properties: velocities, pseudo-equivalent widths, decline rates, magnitudes, time durations, and environment metallicity. Our sample displays a large range in ejecta expansion velocities, from ∼9600 to ∼1500 km s
−1
at 50 days post-explosion with a median
value of 7300 km s
−1
. This is most likely explained through differing explosion energies. Significant diversity is also observed in the absolute strength of spectral lines, characterized through their pseudo-equivalent widths. This implies significant diversity in both temperature evolution (linked to progenitor radius) and progenitor metallicity between different SNe II. Around 60% of our sample shows an extra absorption component on the blue side of the
P-Cygni profile (“Cachito” feature) between 7 and 120 days since explosion. Studying the nature of Cachito, we conclude that these features at early times (before ∼35 days) are associated with Si
ii
, while past the middle of the plateau phase they are related to high velocity (HV) features of hydrogen lines.
The Tampa Scale for Kinesiophobia (TSK) is one of the most frequently employed measures for assessing pain-related fear in pain patients. Although the TSK has been translated into different ...languages, a Spanish version of the TSK has not been available, up to now. Thus, the aim of this study was to validate the Spanish version of the TSK in 2 different pain samples: A heterogeneous chronic pain sample (n = 125) and a musculoskeletal acute pain sample (n = 86). Factor analysis revealed a 2-factor model of 11 items replicated on both samples, named TSK-11. The instrument obtained shows good reliability (internal consistency and stability) and validity (convergent and predictive), with the advantage of brevity. Evidence is provided on discriminant validity between both TSK factors (called Activity Avoidance and Harm). The Harm factor shows the best predictive validity, as it predicts pain persistence, catastrophizing, depression, and pain intensity scores after 6 months. Changes in the Activity Avoidance factor are positively correlated with changes in catastrophizing and anxiety, and negatively associated with changes in functional status. The results of this study point to the relative contribution of both components of pain-related fear to pain adjustment.
This article presents the Spanish version of the TSK. Factor analysis revealed a 2-factor model (called Activity Avoidance and Harm). The version obtained shows good reliability and validity. Results provide clinicians with access to a measure of pain-related fear for Spanish-speaking pain patients, offering the advantage of brevity.
The indication for antimicrobial treatment of asymptomatic bacteriuria (AB) after kidney transplantation (KT) remains controversial. Between January 2011 and December 2013, 112 KT recipients that ...developed one episode or more of AB beyond the second month after transplantation were included in this open‐label trial. Participants were randomized (1:1 ratio) to the treatment group (systematic antimicrobial therapy for all episodes of AB occurring ≤24 mo after transplantation 53 patients) or control group (no antimicrobial therapy 59 patients). Systematic screening for AB was performed similarly in both groups. The primary outcome was the occurrence of acute pyelonephritis at 24‐mo follow‐up. Secondary outcomes included lower urinary tract infection, acute rejection, Clostridium difficile infection, colonization or infection by multidrug‐resistant bacteria, graft function and all‐cause mortality. There were no differences in the primary outcome in the intention‐to‐treat population (7.5% 4 of 53 in the treatment group vs. 8.4% 5 of 59 in the control group; odds ratio OR 0.88, 95% confidence interval CI 0.22–3.47) or the per‐protocol population (3.8% 1 of 26 in the treatment group vs. 8.0% 4 of 50 in the control group; OR 0.46, 95% CI 0.05–4.34). Moreover, we found no differences in any of the secondary outcomes. In conclusion, systematic screening and treatment of AB beyond the second month after transplantation provided no apparent benefit among KT recipients (NCT02373085).
This randomized controlled trial shows no difference in the incidence of acute pyelonephritis between kidney transplant recipients who had all their episodes of asymptomatic bacteriuria treated and those who did not receive antimicrobial treatment for asymptomatic bacteriuria beyond the second month after transplantation. See the editorial from Blumberg on page 2779.
Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of ...collagen VI in articular cartilage. Collagen VI anchors the chondrocyte to the PCM. It has been suggested that co-culture of chondrons with mesenchymal stromal cells (MSCs) might enhance extracellular matrix (ECM) production. This co-culture study investigates whether MSCs help to preserve the PCM and increase ECM production. Primary bovine chondrons or chondrocytes or rat MSCs were cultured alone to establish a baseline level for ECM production. A xenogeneic co-culture monolayer model using rat MSCs (20, 50, and 80%) was established. PCM maintenance and ECM production were assessed by biochemical assays, immunofluorescence, and histological staining. Co-culture of MSCs with chondrons enhanced ECM matrix production, as compared to chondrocyte or chondron only cultures. The ratio 50:50 co-culture of MSCs and chondrons resulted in the highest increase in GAG production (18.5 ± 0.54 pg/cell at day 1 and 11 ± 0.38 pg/cell at day 7 in 50:50 co-culture versus 16.8 ± 0.61 pg/cell at day 1 and 10 ± 0.45 pg/cell at day 7 in chondron monoculture). The co-culture of MSCs with chondrons appeared to decelerate the loss of the PCM as determined by collagen VI expression, whilst the expression of high-temperature requirement serine protease A1 (HtrA1) demonstrated an inverse relationship to that of the collagen VI. Together, this implies that MSCs directly or indirectly inhibited HtrA1 activity and the co-culture of MSCs with chondrons enhanced ECM synthesis and the preservation of the PCM.
Background
The present interdisciplinary consensus review proposes clinical considerations and recommendations for anaesthetic practice in patients undergoing gastrointestinal surgery with an ...Enhanced Recovery after Surgery (ERAS) programme.
Methods
Studies were selected with particular attention being paid to meta‐analyses, randomized controlled trials and large prospective cohort studies. For each item of the perioperative treatment pathway, available English‐language literature was examined and reviewed. The group reached a consensus recommendation after critical appraisal of the literature.
Results
This consensus statement demonstrates that anaesthesiologists control several preoperative, intraoperative and postoperative ERAS elements. Further research is needed to verify the strength of these recommendations.
Conclusions
Based on the evidence available for each element of perioperative care pathways, the Enhanced Recovery After Surgery (ERAS ®) Society presents a comprehensive consensus review, clinical considerations and recommendations for anaesthesia care in patients undergoing gastrointestinal surgery within an ERAS programme. This unified protocol facilitates involvement of anaesthesiologists in the implementation of the ERAS programmes and allows for comparison between centres and it eventually might facilitate the design of multi‐institutional prospective and adequately powered randomized trials.
Abstract
Eridanus II (Eri II) is an ultrafaint dwarf (UFD) galaxy (
M
V
= −7.1) located at a distance close to the Milky Way virial radius. Early shallow color–magnitude diagrams (CMDs) indicated ...that it possibly hosted an intermediate-age or even young stellar population, which is unusual for a galaxy of this mass. In this paper, we present new Hubble Space Telescope/Advanced Camera for Surveys CMDs reaching the oldest main-sequence turnoff with excellent photometric precision and derive a precise star formation history (SFH) for this galaxy through CMD fitting. This SFH shows that the bulk of the stellar mass in Eri II formed in an extremely short star formation burst at the earliest possible time. The derived star formation rate profile has a width at half maximum of 500 Myr and reaches a value compatible with null star formation 13 Gyr ago. However, tests with mock stellar populations and with the CMD of the globular cluster M92 indicate that the star formation period could be shorter than 100 Myr. From the quantitative determination of the amount of mass turned into stars in this early star formation burst ( ∼2 × 10
5
M
⊙
) we infer the number of supernova (SN) events and the corresponding energy injected into the interstellar medium. For reasonable estimates of the Eri II virial mass and values of the coupling efficiency of the SN energy, we conclude that Eri II could be quenched by SN feedback alone, thus casting doubts on the need to invoke cosmic reionization as the preferred explanation for the early quenching of old UFD galaxies.
For the past six years we have carried out a search for massive planets around main sequence and evolved stars in the open cluster M 67, using radial velocity (RV) measurements obtained with HARPS at ...ESO (La Silla), SOPHIE at OHP and HRS at HET. Additional RV data come from CORALIE at the Euler Swiss Telescope. We aim to perform a long-term study on giant planet formation in open clusters and determine how it depends on stellar mass and chemical composition. We report the detection of three new extrasolar planets: two in orbit around the two G dwarfs YBP1194 and YBP1514, and one around the evolved star S364. The orbital solution for YBP1194 yields a period of 6.9 days, an eccentricity of 0.24, and a minimum mass of 0.34 MJup. YBP1514 shows periodic RV variations of 5.1 days, a minimum mass of 0.40 MJup, and an eccentricity of 0.39. The best Keplerian solution for S364 yields a period of 121.7 days, an eccentricity of 0.35 and a minimum mass of 1.54 MJup. An analysis of Hα core flux measurements as well as of the line bisectors spans revealed no correlation with the RV periods, indicating that the RV variations are best explained by the presence of a planetary companion. Remarkably, YBP1194 is one of the best solar twins identified so far, and YBP1194b is the first planet found around a solar twin that belongs to a stellar cluster. In contrast with early reports and in agreement with recent findings, our results show that massive planets around stars of open clusters are as frequent as those around field stars.
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