Three experimental diets in which fish meal was partially (50 and 75%, named PP50 and PP75 diets, respectively) or totally replaced (PP100 diet) by plant protein sources were fed to rainbow trout and ...gilthead sea bream. We studied the effects of these diets on digestive enzymes in comparison to fish fed fish meal diets (FM). A mixture of vegetal ingredients (corn gluten meal, wheat gluten, extruded peas and rapeseed meal) was used to meet the amino acid requirements of the fish. Over a 12-week trial, four groups of the two species were fed one experimental diet twice a day until they showed satiety.
After the growth period, post-prandial protease and α-amylase activities in proximal intestine were measured. Trout fed fish meal as the sole protein source (FM diet) showed a peak in total protease activity 3 h post-feeding; however, trout fed plant protein based diets (PP diets) did not register a peak in activity of these enzymes. In FM fed sea bream, proteolytic activity peaked in the most proximal intestinal segment 6 h post-feeding. This peak was also observed in PP fed sea bream, although the magnitude tended to decrease as the percentage of plant protein increased. This reduction was due to the specific inhibition of chymotrypsin-like bands, although a slight increase in trypsin secretion was detected in the zymograms of this species. The replacement of fish meal by plant protein did not affect α-amylase activity in either trout or sea bream.
In sea bream proximal intestine histology was also studied. The PP100 fed group showed shorter folds and smaller goblet cell population when compared to FM fed group.
In response to feeding the PP75 and PP100 diets, sea bream and trout showed a significant increase in relative intestinal length. Despite this compensatory mechanism, final weights of the two species diminished; however, this decrease exceeded 20% only in the case of groups fed the PP100 diets.
Through combined ferromagnetic resonance, spin pumping, and inverse spin Hall effect experiments in Co|Pt bilayers and Co|Cu|Pt trilayers, we demonstrate consistent values of ℓsfPt=3.4±0.4 nm and ...θSHEPt=0.056±0.010 for the respective spin diffusion length and spin Hall angle for Pt. Our data and model emphasize the partial depolarization of the spin current at each interface due to spin-memory loss. Our model reconciles the previously published spin Hall angle values and explains the different scaling lengths for the ferromagnetic damping and the spin Hall effect induced voltage.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows a fast and reliable bacterial identification from culture plates. Direct analysis of clinical ...samples may increase its usefulness in samples in which a fast identification of microorganisms can guide empirical treatment, such as blood cultures (BC). Three hundred and thirty BC, reported as positive by the automated BC incubation device, were processed by conventional methods for BC processing, and by a fast method based on direct MALDI-TOF MS. Three hundred and eighteen of them yield growth on culture plates, and 12 were false positive. The MALDI-TOF MS-based method reported that no peaks were found, or the absence of a reliable identification profile, in all these false positive BC. No mixed cultures were found. Among these 318 BC, we isolated 61 Gram-negatives (GN), 239 Gram-positives (GP) and 18 fungi. Microorganism identifications in GN were coincident with conventional identification, at the species level, in 83.3% of BC and, at the genus level, in 96.6%. In GP, identifications were coincident with conventional identification in 31.8% of BC at the species level, and in 64.8% at the genus level. Fungaemia was not reliably detected by MALDI-TOF. In 18 BC positive for Candida species (eight C. albicans, nine C. parapsilosis and one C. tropicalis), no microorganisms were identified at the species level, and only one (5.6%) was detected at the genus level. The results of the present study show that this fast, MALDI-TOF MS-based method allows bacterial identification directly from presumptively positive BC in a short time (<30 min), with a high accuracy, especially when GN bacteria are involved.
As far as the authors know, no in-depth comparison has been made between the different performances of the solar photo-Fenton process for the removal of contaminants of emerging concern (CECs) as a ...function of pH. To this end, real WWTP secondary effluents were treated in continuous flow mode at pilot plant scale. The effect of hydraulic residence time (HRT), liquid depth and percentage of CEC removal on treatment capacity was studied. At acidic pH (2.8), the iron source was FeSO4 and at neutral pH (7.0), it was Fe(III)-EDDS. At both pH values, 2250 L m−2 d−1 can be treated in 15-cm deep raceway pond reactors at 30 min HRT with 0.1 mM iron and 0.88 mM H2O2 in order to achieve 80% CEC removal. Treatment costs were 0.25 € m−3 and 0.56 € m−3 at acidic and neutral pH, respectively. This study paves the way for the solar photo-Fenton process to be employed on a commercial scale.
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•In-depth comparison between acidic and neutral pH solar photo-Fenton for CEC removal•At acidic pH, the iron source was FeSO4 and at neutral pH, the complex Fe(III)-EDDS.•The effect of HRT, liquid depth and CEC removal goal on treatment capacity was studied.•2250 L m−2 d−1 treated in 15-cm deep RPRs at 30 min of HRT, 0.1 mM Fe, 0.88 mM H2O2•Unitary costs were estimated as 0.25 € m−3 and 0.56 € m−3 at acidic and neutral pH.
The Rashba effect is an interaction between the spin and the momentum of electrons induced by the spin-orbit coupling (SOC) in surface or interface states. Its potential for conversion between charge ...and spin currents has been theoretically predicted but never clearly demonstrated for surfaces or interfaces of metals. Here we present experiments evidencing a large spin-charge conversion by the Bi/Ag Rashba interface. We use spin pumping to inject a spin current from a NiFe layer into a Bi/Ag bilayer and we detect the resulting charge current. As the charge signal is much smaller (negligible) with only Bi (only Ag), the spin-to-charge conversion can be unambiguously ascribed to the Rashba coupling at the Bi/Ag interface. This result demonstrates that the Rashba effect at interfaces can be used for efficient charge-spin conversion in spintronics.
In this study, we found that in the adipose tissue of wildtype animals, insulin and TGF‐β signalling converge via a BMP antagonist short gastrulation (sog) to regulate ECM remodelling. In tumour ...bearing animals, Sog also modulates TGF‐β signalling to regulate ECM accumulation in the fat body. TGF‐β signalling causes ECM retention in the fat body and subsequently depletes muscles of fat body‐derived ECM proteins. Activation of insulin signalling, inhibition of TGF‐β signalling, or modulation of ECM levels via SPARC, Rab10 or Collagen IV in the fat body, is able to rescue tissue wasting in the presence of tumour. Together, our study highlights the importance of adipose ECM remodelling in the context of cancer cachexia.
Synopsis
During cancer cachexia, insulin and TGF‐β signalling converge in the fat body via Sog to hinder ECM secretion, which in turn affects muscle integrity.
Eye tumour models employing RasV12 together with Scrib RNAi or Dlg RNAi were used to study how inter‐organ communication affects fat body and muscle integrity in cancer cachexia.
TGF‐β and insulin signalling converge in the fat body to control ECM secretion via Sog.
Modulating ECM secretion from the fat body rescues muscle integrity in the presence of tumour.
During cancer cachexia, insulin and TGF‐β signalling converge in the fat body via Sog to hinder ECM secretion, which in turn affects muscle integrity.
High performance sport for females is an area which is gaining more and more relevance today, but which hasn't received the same research interest as sport for males. The aim of the present study was ...to analyse the motivational climate perceived by high performance female athletes and the implications on perceived motor competence and autonomous behaviour and check the differences according category, sport experience and training hours in performance and master climate. The participants were 615 female athletes who practice top level team sports, with ages comprised of 16 to 38 (M = 22,10; SD = 4,91). The sample consisted of two different categories: junior (n = 242) and senior (n = 373). These players participated in different team sports, specifically football, handball, basketball and volleyball, training between 6 and 24 hours a week (M = 8,34; DT = 4,33). The variables measured were: perceived motivational climate in sport, autonomous behaviour and perceived motor competence. A cluster analysis was carried out using, as a variable, the perceived motivational climate, and showing the existence of two profiles, one related to ego and the other to task. The multivariate analysis showed that the profile orientated towards the task had significant differences compared to the autonomous behaviour (M = 4.66 vs M = 3.41). At the same time an analysis was carried out looking at different social demographic variables, revealing how there were differences in the sports experience (those participants with more than ten years' experience were more orientated towards ego, compared to those with less than ten years' experience) and the category (junior athletes were more orientated towards the task, compared to senior athletes). It was concluded that a greater orientation towards the task can lead to an improvement in the perception of motor competence, with the youngest and least experienced athletes being the most prominent in this category.
We present results on spin to charge current conversion in experiments of resonant spin pumping into the Dirac cone with helical spin polarization of the elemental topological insulator (TI) α-Sn. By ...angle-resolved photoelectron spectroscopy (ARPES), we first check that the Dirac cone (DC) at the α-Sn (0 0 1) surface subsists after covering Sn with Ag. Then we show that resonant spin pumping at room temperature from Fe through Ag into α-Sn layers induces a lateral charge current that can be ascribed to the inverse Edelstein effect by the DC states. Our observation of an inverse Edelstein effect length much longer than those generally found for Rashba interfaces demonstrates the potential of TIs for the conversion between spin and charge in spintronic devices. By comparing our results with data on the relaxation time of TI free surface states from time-resolved ARPES, we can anticipate the ultimate potential of the TI for spin to charge conversion and the conditions to reach it.
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