Cervical cancer is the third female cancer most common worldwide. The carcinogenic process involves an alteration of the mechanisms associated with transcription. Several studies have reported an ...oncogenic role of the polycomb complex subunit, EZH2. However, the role of EZH2 in cervical cancer is unknown. Hence, the objective of this study was to determine the role of EZH2 in transcriptional regulation in cervical cancer. The EZH2 expression and the methylation status of its promoter were analyzed in The Cancer Genome Atlas. The EZH2 enrichment profile was analyzed using chromatin immunoprecipitation with massively parallel DNA sequencing data provided by ENCODE project. The chromatin compartments were identified in the 4D Nucleome Data Portal. The functional annotation was examined in Enrichr. We report that EZH2 expression is increased in cervical cancer which is associated with hypomethylation of its promoter. EZH2 is enriched at promoter and distal intergenic regions. We identified that EZH2 defines chromatin domains enriched with H3K27me3 within repressive compartments in the HeLa-S3 cell line. Additionally, high EZH2 expression is associated with the repression of the senescent phenotype in cervical cancer patients. Our results suggest the participation of EZH2 in the generation of domains with a silencer function in cervical cancer, which regulate the expression of genes associated with cellular senescence.
This paper introduces a systematic approach for water integration in industrial facilities based on properties. New property operators and mixing rules (e.g., for odor and toxicity) are proposed to ...enable the tracking of stream characteristics and the inclusion in an optimization model. Additionally, the dependence of the properties on temperature is accounted for. The thermal effects upon properties are also considered in the proposed water-network model by including cooling and heating. The proposed model for water integration is based on a recycle scheme that includes regeneration processes to improve the stream properties. Furthermore, temperature-adjusting property interceptors are included in the superstructure of the regeneration network to satisfy the temperature constraints in the sinks and also to improve temperature-dependent properties. The applicability of the proposed approach is shown through several case studies, where methodology comparison is made with respect to the ones previously reported in the literature.
Identifying edentulous victims in forensic contexts poses a significant challenge. It has been reported that having a denture to reproduce and compare Palatal Rugae (PR) patterns is crucial for ...identifying edentulous individuals, yet there are no validated protocols for conducting this procedure. In this study, a new method was developed and validated for plaster molding of the internal surface of upper dentures, along with a protocol involving focus-stacked photographs and reference scales. Thirty-eight edentulous subjects participated in the study, obtaining plaster models of the patients (PM), and their dentures (AM) were standardized. The AM/PM model was highlighted and photographed with ten shots in different z-focuses for each model, using standard lighting and aligning the position of the PR perpendicular to the photographic axis using tubular spirit levels and modeling clay. The images were processed using the stacking technique and analyzed by three observers through the proposed protocol using Adobe® Photoshop®. The results were analyzed based on the intra-observer and inter-observer agreement levels, with a 95% confidence interval. This study demonstrated high-precision intra-observer and inter-observer agreement (k = 1) in the matching of Palatal Rugae (PR) and maxillary morphology obtained from participants and their dentures. The protocol is simple, cost-effective, and precise. It enables standardization of the technique for obtaining plaster models, and the exposure of PR and photographic protocol minimizes the presence of artifacts in the images, thereby reducing the likelihood of errors and promoting the reproducibility of both the recording technique and the comparison of the PR.Identifying edentulous victims in forensic contexts poses a significant challenge. It has been reported that having a denture to reproduce and compare Palatal Rugae (PR) patterns is crucial for identifying edentulous individuals, yet there are no validated protocols for conducting this procedure. In this study, a new method was developed and validated for plaster molding of the internal surface of upper dentures, along with a protocol involving focus-stacked photographs and reference scales. Thirty-eight edentulous subjects participated in the study, obtaining plaster models of the patients (PM), and their dentures (AM) were standardized. The AM/PM model was highlighted and photographed with ten shots in different z-focuses for each model, using standard lighting and aligning the position of the PR perpendicular to the photographic axis using tubular spirit levels and modeling clay. The images were processed using the stacking technique and analyzed by three observers through the proposed protocol using Adobe® Photoshop®. The results were analyzed based on the intra-observer and inter-observer agreement levels, with a 95% confidence interval. This study demonstrated high-precision intra-observer and inter-observer agreement (k = 1) in the matching of Palatal Rugae (PR) and maxillary morphology obtained from participants and their dentures. The protocol is simple, cost-effective, and precise. It enables standardization of the technique for obtaining plaster models, and the exposure of PR and photographic protocol minimizes the presence of artifacts in the images, thereby reducing the likelihood of errors and promoting the reproducibility of both the recording technique and the comparison of the PR.
•An optimization framework for designing macroscopic water systems is proposed.•Optimal planning and scheduling for water storage and distribution is considered.•The model incorporates several ...options for water recycling, reusing and collecting.•Future projections are considered in the model.•A case study for the city of Morelia in Mexico is presented.
Water scarcity is one of the main concerns of several countries around the world. In this context, several approaches have been proposed for resource conservation and available water augmentation through specific actions such as process intensification and the use, reclamation, reuse, and recycle of alternative water sources. Nonetheless, there are no reported methodologies optimizing the multiannual planning of water usage, discharge, reclamation, storage and distribution in a macroscopic system considering natural and alternative water sources. In this paper, a multi-period mathematical programming model for the optimal planning of water storage and distribution in a macroscopic system is presented. The model addresses important factors such as population growth, change in the time value of money and change in the precipitation patterns. The proposed model is applied to the case of a Mexican city. The results show important advantages from the economic and sustainability points of view.
Batoid (rays and skates) populations are declining worldwide, with unknown ecological consequences due to lacking consolidated data on the trophic ecology of these species. Such trends are ...particularly disconcerting in Mexican waters, where batoids are heavily exploited by commercial fisheries. To assess the current state of knowledge of batoid diet in this region, we conducted a meta‐analysis of 54 published stomach content analysis studies. Trophic niche was assessed from 44 total species, including variations due to sex, ontogeny, season, and region, as well as trophic overlap among species. The species assessed belonged to the taxonomic families Urotrygonidae, Dasyatidae, Potamotrygonidae, Mobulidae, Rhinopteridae, Aetobatidae, Gymnuridae, Rhinobatidae, Rajidae, Arhyncobatidae, and Narcinidae. Most of the diet studies to date were conducted in the Mexican Pacific (n = 24), whereas only three studies have come from the Mexican Atlantic, with 27 additional studies coming from other American countries from the tropical and subtropical region. Crustaceans were reported in more than 50% of the species assessed and were also generally the most important prey item, with either high (76%–100%) and medium‐high (51%–75%) importance based on dietary indices from the literature reviewed. While the diet of 40 species (91%) consisted of more than one prey type, feeding strategy analysis (Levin and Shannon–Wiener indices) of 27 species indicated that 25 were specialists and two generalists. Species diets varied with ontogeny (20%), sex (11%), region (11%), and seasonality (9%). According to the reviewed studies, interspecific diet overlap was evident in 36% of species, mainly in the family Urotrygonidae. Batoids were grouped into four trophic guilds: crustacivores (68.1%), annelidivores (primarily polychaetes) (13.6%), molluscivores (11.3%), and piscivores (6.8%), based on the literature reviewed. This study showed that most of the batoids had a specialist crustacean‐based diet. Future research should focus on species devoid of dietary data to encapsulate the trophic niche breadth of this group in Mexican waters, particularly from the Mexican Atlantic and surrounding regions.
Ecologists examine diet composition in order to assess the spatial and temporal variations in interactions between species, the impact of different species traits on the ecological network structure, ...and the long-term effects of the removal of different species by small-scale fisheries. In this study, our goal was to compare the diets of silky sharks (Carcharhinus falciformis) and scalloped hammerhead sharks (Sphyrna lewini) off the south-west coast of Mexico in order to infer their diet preferences and spatial distributions. We sampled 164 S. lewini (96 stomachs had food, 68 were empty) and 183 C. falciformis (30 stomachs had food, 153 were empty) in Puerto Madero, Chiapas in 2011. The large number of empty stomachs may be the result of using longline fishing gear, which causes high stress resulting in regurgitation. Based on the index of relative importance (%IRI), the fish Chloroscombrus orqueta (IIR = 27.7%) was the most important species in the diet of S. lewini, while the squid Dosidicus gigas (IIR = 34%) was the primary prey of C. falciformis. Levin's index (Bi) and Shannon's index (H′) confirm that both sharks are generalists, as in other regions. The trophic levels of S. lewini (TL = 4.1) and C. falciformis (TL = 4.2) are characteristic of tertiary consumers; meanwhile, the Morisita–Horn index indicates low interspecific overlap between all categories. These results confirm that these two sharks have different foraging preferences or movement patterns; thus, there is no trophic overlap between species as they play unique roles in the ecological network off the south-west coast of Mexico.
The aim of this article was to analyze the evidence regarding the effectiveness of intervention programs for children with autism based on the participation of their parents. To obtain the data, a ...systematic search was carried out in four databases (PsycARTICLES (ProQuest), ERIC (ProQuest), PubMed (ProQuest), and Scopus). The retrieved documents were refined under the inclusion/exclusion criteria, and a total of 51 empirical studies were selected. These studies were first classified according to the function of the intervention objective and, later, by the methodology applied (19 studies were based on comprehensive interventions, 11 focused on the nuclear symptoms of autism spectrum disorder (ASD), 12 focused on the promotion of positive parenting, and nine interactions focused on child play). Once all of the documents had been analyzed, the evidence indicated scientific efficacy in most studies, mainly in those based on child development and the application of behavioral analysis principles. Moreover, the positive influence of parent participation in such programs was demonstrated.
Green tomato (
) is a specie native to Mexico, and it is known as "tomatillo" or "husk tomato". The fruit contains vitamins, minerals, phenolic compounds, and steroidal lactones, presenting ...antimicrobial activity and antinarcotic effects. Therefore, it is not only used in traditional Mexican cuisine, but also in traditional medicine to relieve some discomforts such as fever, cough, and amygdalitis. However, it is a perishable fruit whose shelf life is very short. As a part of the peel, cuticle, and epicuticular waxes represent the most important part in plant protection, and the specific composition and structural characterization are significant to know how this protective biopolymer keeps quality characteristics in fresh fruits.
cutin was obtained by enzymatic treatments (cellulase, hemicellulose, and pectinase) and different concentrations of TFA, and studied through Cross Polarization Magic Angle Spinning Nuclear Magnetic Resonance (CPMAS
C NMR), Ultra-High Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS), and was morphologically characterized by Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). The main constituents identified under the basis of UHPLC-MS analysis were 9,10,18-trihydroxy-octadecanoic acid and 9,10-epoxy-18-hydroxy-octadecanoic acid with 44.7 and 37.5%, respectively. The C16 absence and low occurrence of phenolic compounds, besides the presence of glandular trichomes, which do not allow a continuous layer on the surface of the fruit, could be related to a lower shelf life compared with other common fruits such as tomato (
).
Environmental changes have been associated with natural climatic variability or human activity. Water resources management is, perhaps, the most drastic change observed in the coastal environment. ...However, external forcings such as the El Niño event have important implications in the global and regional hydrological balance. These environmental changes have an impact on the density and biomass of the ichthyofauna in the Terminos Lagoon (TL) for the past 30 years, presumably, associated with variations in the temperature and surface salinity of the sea. Therefore, in the present study, δ18O was quantified in otoliths of two important species due to their dominance: Stellifer lanceolatus and Eucinostomus gula, and to understand the environmental changes reflected in both species. The δ18O was analyzed in otoliths of these two species captured in 1998/1997, 2006/2007 and 2016/2017 and were compared with in situ temperature and salinity data. Sea surface temperature and salinity increased by 2 °C and 9, respectively, between 1997 and 2017. Stellifer lanceolatus δ18O values was in isotopic equilibrium with seawater calcite; while, E. gula is not in isotopic equilibrium. The δ18O of S. lanceolatus and E. gula varied significantly with the increase in salinity (R2 = 0.8987 and R2 = −0.2964) and not with the sea surface temperature. S. lanceolatus is an excellent bioindicator of changes in sea surface salinity in this region of the Gulf of Mexico.
•δ18O in Stellifer lanceolatus was in isotopic equilibrium with seawater calcite.•δ18O in Eucinostomus gula wasn't in isotopic equilibrium with seawater calcite.•δ18O of both species varied significantly with the increase in salinity.•S. lanceolatus is an excellent bioindicator of changes in salinity.
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