Fundamentação: A fibrose cística (FC) é uma das doenças genéticas letais mais comuns. Nos últimos anos, a sobrevida dos pacientes portadores dessa doença tem aumentado em decorrência principalmente ...do uso de antibióticos profiláticos e terapêuticos. Estes apresentam potencial ototóxico, sendo responsáveis por perdas auditivas sensorioneurais (SSN). Além disso, os pacientes com FC apresentam rinossinusopatias, sintomáticas e assintomáticas, que também necessitam de acompanhamento adequado. Objetivos: Definir a prevalência de alterações auditivas sensorioneurais (SSN) e rinossinusopatias nos pacientes com FC do Hospital de Clínicas de Porto Alegre (HCPA); descrever os possíveis fatores relacionados com tais incidências; e, a partir dessas informações, projetar o funcionamento do ambulatório de otorrinolaringologia (ORL) e FC do HCPA. Material e Métodos: Estudo retrospectivo, em que foram revisados os prontuários de pacientes com FC do Ambulatório de Pneumologia Infantil e de Adultos do HCPA. Foi criado um protocolo a ser preenchido em que se buscava a descrição do perfil da amostra em relação à idade do diagnóstico da doença, idade atual, tipos de antibióticos já utilizados, média do número de tratamentos, percentual que possui avaliação auditiva com resultados, e, por último, investigação radiológica e resultados. Resultados: Foram revisados 107 prontuários de pacientes com FC. A idade média da amostra foi de 7,87 anos e 1,33 anos a idade média de diagnóstico de FC. Os exames audiométricos foram realizados por 39,3% (42) das crianças, encontrando-se 28,56% (12) casos de SSN de graus variados. Um percentual mais elevado apresentava ausência de reflexo estapédico (36,3%). Somente 24,3% (26) realizaram RX dos seios da face nos quais 96.9% estavam alterados. Um total de 667 tratamentos com aminoglicosídeos, sendo 49.5% com tobramicina, 43.4% com amicacina e 7% com gentamicina. Conclusão: Diante desses resultados mostra-se necessário um ambulatório específico de otorrinolaringologia e de FC para acompanhamento desses pacientes. O Ambulatório terá como objetivo auxiliar na prevenção e tratamento das perdas auditivas decorrentes dos tratamentos com aminoglicosídeos, assim como tratar, quando necessário, das vias aéreas superiores em relação à obstrução e inflamação associadas a presença de pólipos rinossinusais. Este ambulatório já está em funcionamento.
Cystic fibrosis (CF) is considered one of the most prevalent lethal genetic disorders. The life expectancy of those patients has been rising. Besides the well-known prevalence of chronic ...rhinosinusitis, neurosensory hearing loss (NSHL) due to the large use of ototoxic antibiotics may be another problem that should receive special attention in otolaryngology (ENT).
Define retrospectively the prevalence of NSHL among patients with CF from the Hospital de Clínicas de Porto Alegre (HCPA) and based on this data discuss the need for a specific out patient ENT-CF clinic.
Files from CF HCPA patients were reviewed for age at diagnosis, the current age, types of antibiotics used, number of treatments, percentage with audiologic evaluation and its respective results.
One hundred and seven files were reviewed. The mean age at diagnosis was 1.33 years old (SD 1.97, range 0-12). The mean age of the sample was 7.87 (SD 4.49, range 1-22). Audiologic evaluation was performed in 39.3% (42) of the patients. 28.56% (12) had some degree of NSHL, while 36.3% did not present acoustic reflex. From 667 treatments with antibiotics, 49.5% were with tobramycin, 43.4% with amycacin and 7% with gentamicin.
The prevalence of NSHL among CF patients supports the need for a specific ENT-CF outpatient clinic. This clinic would help on the prevention and treatment of NSHL due to the use of ototoxic antibiotics and on the better understanding/control of rhinosinusal disorders.
Cystic fibrosis (CF) is considered one of the most prevalent lethal genetic disorders. The life expectancy of those patients has been rising. Besides the well-known prevalence of chronic ...rhinossinusitis, neurossensorial hearing loss (NSHL) due to the large use of ototoxic antibiotics may be another problem that should receive special attention in otolaryngology (ENT).
Objectives: Define retrospectively the prevalence of NSHL among patients with CF from the Hospital de Clı́nicas de Porto Alegre (HCPA) and based on this data discuss the need for a specific out patient ENT–CF clinic.
Material and method: Files from CF HCPA patients were reviewed for age at diagnosis, the current age, types of antibiotics used, number of treatments, percentage with audiologic evaluation and its respective results.
Results: One hundred and seven files were reviewed. The mean age at diagnosis was 1.33 years old (SD 1.97, range 0–12). The mean age of the sample was 7.87 (SD 4.49, range 1–22). Audiologic evaluation was performed in 39.3% (42) of the patients. 28.56% (12) had some degree of NSHL, while 36.3% did not present acoustic reflex. From 667 treatments with antibiotics, 49.5% were with tobramicyn, 43.4% with amycacin and 7% with gentamicin.
Conclusions: The prevalence of NSHL among CF patients supports the need for a specific ENT–CF outpatient clinic. This clinic would help on the prevention and treatment of NSHL due to the use of ototoxic antibiotics and on the better understanding/control of rhinossinusal disorders.
New palladacyclopentadienyl complexes with bis-N-heterocyclic carbenes as spectator ligands have been synthesized and exhaustively characterized. The crystal structure of complex 1a has been also ...determined by X-ray diffraction analysis. Their in vitro cytotoxicity and that of other palladacyclopentadienyl derivatives coordinating different ancillary ligands has been determined against different cancer cell lines. Many complexes have shown an antiproliferative activity toward tumor cells often definitely better than cisplatin, whereas they have resulted practically inactive against the non-cancer MRC-5 cell line. The mechanism of action of bis-NHC derivative 1a, particularly active against ovarian cancer cell lines was studied in depth. Through a longitudinally analysis, it is shown that compound 1a induces apoptosis via DNA damage and release of cytochrome C. We propose compound 1a as a powerful and specific drug for the therapy of a deadly disease such as high grade serous ovarian cancer.
Palladacyclopentadienyl complexes with N-heterocyclic carbene as spectator ligands show a selective and potent antiproliferative activity against different tumor cells lines and in particular toward ovarian cancer cells, with induction of apotosis via DNA damage and release of cytochrome C. Display omitted
•New palladacyclopentadienyl complexes with N-Heterocyclic carbenes as spectator ligands have been synthetized.•Many complexes have shown an antiproliferative activity toward tumor cells often better than cisplatin.•Compound 1a induces apoptosis via DNA damage and release of cytochrome C.•We propose compound 1a as a potent and specific drug for the therapy of high grade serous ovarian cancer.
A general synthetic entryway into novel cationic Pd(II) indenyl complexes bearing one alkyl/aryl phosphine and one N‐heterocyclic carbene is reported. All metal complexes have been exhaustively ...characterized by spectroscopic and structural analyses, highlighting that the indenyl fragment has an hapticity intermediate between η3 and η5. Most of the target complexes are stable in solid state and in solution for a long time. Two different applications of these organopalladium compounds are proposed. Firstly, they have been tested as antiproliferative agents towards three different ovarian cancer cell lines, showing a cytotoxicity significantly higher than that of cisplatin, with a clear dependence on the nature of the coordinated phosphine. Moreover, the similar cytotoxicity towards cisplatin‐sensitive and cisplatin‐resistant cell lines suggests that these new palladium derivatives act with a different mechanism of action with respect to classical platinum‐based drugs.
Finally, the water‐soluble palladium complexes bearing 1,3,5‐triaza‐7‐phosphaadamantane (PTA) have demonstrated interesting catalytic performances in Suzuki–Miyaura coupling in aqueous media, being, inter alia, readily and efficiently recyclable.
Palladium indenyl complexes bearing phosphine and NHC ligands: The synthesis and characterization of novel Pd(II)‐indenyl complexes bearing phosphine and NHC ligands is reported. Some of these derivatives, showed good antitumor activity and catalytic performances in aqueous media.
A series of new palladium allyl complexes bearing purine-based carbenes derived from caffeine, theophylline and theobromine have been prepared and characterized by NMR spectroscopy, and elemental ...analysis and in two cases by single crystal X-ray diffraction. The cytotoxic and proapoptotic activities of compounds have been determined in vitro on human ovarian cancer A2780 and SKOV-3 cell lines. These experiments have shown that the palladium-allyl fragment induces a general cytotoxicity, but the choice of the supporting ligands is of paramount importance for achieving the best results. In particular complexes 4c, 4d and 5d exhibit a higher antiproliferative effect (IC50: 0.09, 0.81 and 0.85 μM respectively) than cisplatin (IC50: 1.5 μM) on A2780 cells, and 4d (IC50: 1.7 μM vs. 5.94 μM) on SKOV-3 cell line. Moreover in many cases it has been proved that the cytotoxicity of our complexes is associated with the induction of apoptosis.
A new dinuclear Pd(i) complex coordinating two bis(NHC) ligands revealed an unsuspected stability despite the unsaturation of the two metal centres. Even more surprisingly, the compound showed high ...and selective antiproliferative activity against different cancer cell lines and ovarian cancer tumoroids, and the mechanism of action was different from that of cisplatin.
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