High-resolution mapping and monitoring of national land use/cover changes contribute significantly to the knowledge of the interaction between human activities and environmental changes. China’s Land ...Use/cover Dataset (CLUD) for 2020 and its dynamic changes in 2015–2020 were developed to extend the CLUD to over 30 years (i.e., the 1980s to 2020 at 5-year intervals) by integrating remote sensing big data and knowledge-based human-computer interaction interpretation methods. This integrating method for CLUD 2020 improved the efficiency of national land use/cover mapping and the accuracy of land use pattern change detection compared to earlier CLUD products, with an overall accuracy of 95%. The intensity of land use change decreased across China in 2015–2020 compared to 2010–2015, although both characteristics of its spatial changes were similar. The cropland area continued to shrink at national scale in 2015–2020, with two regional hotspots including the widespread conversions from dry land into paddy land in Northeast China and the coexistence of widespread land cultivation and cropland abandonment in Xinjiang of Northwest China. Built-up land area continued to expand in China, showing consistency between 2015–2020 and 2010–2015, in which hotspots transited from the surroundings of coastal megacities to the city surroundings of the central and western zones. For natural land, although the woodland and grassland decreased in 2015–2020, its magnitude expanded compared to 2010–2015. In comparison, the water body area in Qinghai-Tibet Plateau increased significantly under the continuous impact of climate change. These characteristics of land use change were closely related to the development strategy of the top-level design of the 13th Five-Year Plan (2016–2020) (e.g., ecological civilization construction and high-quality development).
Abstract
Many of the currently available COVID-19 vaccines and therapeutics are not effective against newly emerged SARS-CoV-2 variants. Here, we developed the metallo-enzyme domain of angiotensin ...converting enzyme 2 (ACE2)—the cellular receptor of SARS-CoV-2—into an IgM-like inhalable molecule (HH-120). HH-120 binds to the SARS-CoV-2 Spike (S) protein with high avidity and confers potent and broad-spectrum neutralization activity against all known SARS-CoV-2 variants of concern. HH-120 was developed as an inhaled formulation that achieves appropriate aerodynamic properties for rodent and monkey respiratory system delivery, and we found that early administration of HH-120 by aerosol inhalation significantly reduced viral loads and lung pathology scores in male golden Syrian hamsters infected by the SARS-CoV-2 ancestral strain (GDPCC-nCoV27) and the Delta variant. Our study presents a meaningful advancement in the inhalation delivery of large biologics like HH-120 (molecular weight (MW) ~ 1000 kDa) and demonstrates that HH-120 can serve as an efficacious, safe, and convenient agent against SARS-CoV-2 variants. Finally, given the known role of ACE2 in viral reception, it is conceivable that HH-120 has the potential to be efficacious against additional emergent coronaviruses.
Prostate cancer (PC), a common malignant tumor, is the second-leading cause of cancer death among American men. Its successful treatment greatly relies on the early diagnose. Engrailed-2 (EN2) has ...been confirmed being existed with a high level in the urine of PC patients. In this study, to explore the application of EN2 in PC, we detected the immunohistochemical staining difference and EN2 expression level between benign prostatic hyperplasia (BPH) and PC.
We developed a monoclonal antibody against the helix 3 in EN2 and confirmed its specificity with Western blotting (WB) and immunofluorescence detecting the subcellular localization of endogenous and exogenous EN2 in three PC cell lines (LNCap, PC3, and DU145). We conducted immunohistochemical staining using this homemade antibody, and RT-PCR to detect the expression of EN2 in 25 PC and 25 BPH cases, and analyzed the correlation of EN2 expression and PC clinical staging.
The results of WB and immunofluorescence showed our homemade EN2 monoclonal antibody could specifically bind endogenous and exogenous EN2 protein in three different PC cell lines. Endogenous EN2 was generally expressed in the cytoplasm and exogenous EN2 mostly existed in the nucleus of these cell lines. Immunohistochemical staining in PC had extremely stronger signals than that in BPH, suggesting a higher EN2 expression level in PC, which was confirmed by RT-PCR. Interestingly, the stained areas in BPH tissues were mainly in nucleus and cytoplasm, while in PC tissues were mainly on cytomembrane. Moreover, the expression level of EN2 was positively correlated with the PC clinical staging.
Using our homemade EN2 antibody, we have found different staining patterns and expression level of EN2 in BPH and PC,which may be helpful to predict prostatic disease progression.
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