The contamination patterns and fate of pharmaceutically active compounds (PhACs) were investigated in the Evrotas River (Southern Greece). This is a temporary river with differing levels of water ...stress and water quality impairment in a number of its reaches. Three sampling campaigns were conducted in order to capture different levels of water stress and water quality. Four sampling sites located on the main channel of the Evrotas River were sampled in July 2015 (moderate stream flow), and June and September 2016 (low stream flow). Discharge of urban wastewater has been determined as the main source of pollution, with PhACs, nutrients and other physicochemical parameters considerably increasing downstream the wastewater treatment plant (WWTP) of Sparta city. Due to the pronounced hydrological variation of the Evrotas River, generally, the highest concentrations of PhACs have been detected during low flow conditions. Simultaneously, low flow resulted in an increased water travel time and consequently longer residence time that accounted for the higher attenuation of most PhACs. The average decrease in total concentration of PhACs within the studied waterbody segment (downstream of Sparta city) increased from 22% in July 2015 to 25% in June 2016 and 77% in September 2016. The PhACs with the highest average concentration decrease throughout the sampling campaigns were hydrochlorothiazide, followed by sotalol, carbamazepine, valsartan, and naproxen.
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•Variation of PhAC and nutrient concentrations relates to river flow variability.•PhACs and nutrients are considerably higher downstream of the WWTP Sparta.•Longer residence times accounts for higher in-stream attenuation of most PhACs
Human appropriation of water resources may induce water stress in freshwater ecosystems when ecosystem needs are not met. Intensive abstraction and regulation cause river ecosystems to shift towards ...non-natural flow regimes, which might have implications for their water quality, biological structure and functioning. We performed a meta-analysis of published studies to assess the potential effects of water stress on nutrients, microcontaminants, biological communities (bacteria, algae, invertebrates and fish), and ecosystem functions (organic matter breakdown, gross primary production and respiration). Despite the different nature of the flow regime changes, our meta-analysis showed significant effects of human-driven water stress, such as significant increases in algal biomass and metabolism and reduced invertebrate richness, abundance and density and organic matter decomposition. Water stress also significantly decreased phosphate concentration and increased the concentration of pharmaceutical compounds. The magnitude of significant effects was dependent on climate, rainfall regime, period of the year, river size and type of water stress. Among the different causes of water stress, flow regulation by dams produced the strongest effects, followed by water abstraction and channelization.
Temporary streams are submitted to high seasonal hydrological variations which induce habitat fragmentation. Global change promotes longer non-flow periods, affecting hydrological continuity and the ...distribution of biological assemblages in river networks. We aimed to investigate the effects of hydrological discontinuity on phototrophic biofilm assemblages in a Mediterranean stream, at both network and habitat scales. At the network scale during basal flow conditions, mostly nitrate and DOC concentrations were associated to the taxonomical and trait distribution of algae and cyanobacterial assemblages. Cyanobacteria dominated at the upstream and downstream sites of the network, while green algae and diatoms were abundant in its middle part. At the habitat scale, hydrological discontinuity promoted large changes in biofilm composition between riffles and pools, where pools were inhabited preferentially by green algae and riffle habitats by cyanobacteria. Our findings emphasize the myriad of factors affecting the spatial distribution of phototrophic biofilms, which become more heterogeneous according to water flow interruption. Under the predicted climate change scenarios, spatial heterogeneity in temporary streams may increase, which will lead to change phototrophic biofilm assemblages.
Spatial heterogeneity along river networks is interrupted by dams, affecting the transport, processing, and storage of organic matter, as well as the distribution of biota. We here investigated the ...structure of planktonic (free-living, FL), particle-attached (PA) and sediment-associated (SD) bacterial and archaeal communities within a small reservoir. We combined targeted-amplicon sequencing of bacterial and archaeal 16S rRNA genes in the DNA and RNA community fractions from FL, PA and SD, followed by imputed functional metagenomics, in order to unveil differences in their potential metabolic capabilities within the reservoir (tail, mid, and dam sections) and lifestyles (FL, PA, SD). Both bacterial and archaeal communities were structured according to their life-style preferences rather than to their location in the reservoir. Bacterial communities were richer and more diverse when attached to particles or inhabiting the sediment, while Archaea showed an opposing trend. Differences between PA and FL bacterial communities were consistent at functional level, the PA community showing higher potential capacity to degrade complex carbohydrates, aromatic compounds, and proteinaceous materials. Our results stressed that particle-attached prokaryotes were phylogenetically and metabolically distinct from their free-living counterparts, and that performed as hotspots for organic matter processing within the small reservoir.
The extensive use of antibiotics in human and veterinary medicine and their subsequent release into the environment may have direct consequences for autochthonous bacterial communities, especially in ...freshwater ecosystems. In small streams and rivers, local inputs of wastewater treatment plants (WWTPs) may become important sources of organic matter, nutrients and emerging pollutants, such as antibiotic resistance genes (ARGs). In this study, we evaluated the effect of WWTP effluents as a source of ARGs in river biofilms. The prevalence of genes conferring resistance to main antibiotic families, such as beta-lactams (blaCTX-M), fluoroquinolones (qnrS), sulfonamides (sul I), and macrolides (ermB), was determined using quantitative PCR (qPCR) in biofilm samples collected upstream and downstream WWTPs discharge points in four low-order streams. Our results showed that the WWTP effluents strongly modified the hydrology, physico-chemistry and biological characteristics of the receiving streams and favoured the persistence and spread of antibiotic resistance in microbial benthic communities. It was also shown that the magnitude of effects depended on the relative contribution of each WWTP to the receiving system. Specifically, low concentrations of ARGs were detected at sites located upstream of the WWTPs, while a significant increase of their concentrations was observed in biofilms collected downstream of the WWTP discharge points (particularly ermB and sul I genes). These findings suggest that WWTP discharges may favour the increase and spread of antibiotic resistance among streambed biofilms. The present study also showed that the presence of ARGs in biofilms was noticeable far downstream of the WWTP discharge (up to 1 km). It is therefore reasonable to assume that biofilms may represent an ideal setting for the acquisition and spread of antibiotic resistance determinants and thus be considered suitable biological indicators of anthropogenic pollution by active pharmaceutical compounds.
•We evaluated the effect of WWTP effluents on small rivers.•We investigated the occurrence of antibiotic resistance genes (ARGs) in biofilms.•WWTP effluents deeply modified the characteristics of the receiving streams.•WWTP effluents favoured the spread and persistence of ARGs in biofilms.•The increase of ARGs in biofilms was noticeable far downstream of the WWTP discharge.
The study of biofilms in rivers revealed that small wastewater treatment plants can be a relevant source of antibiotic resistance genes to benthic communities of freshwater ecosystems.
A tale of pipes and reactors Casas-Ruiz, Joan P.; Catalán, Núria; Gómez-Gener, Lluís ...
Limnology and oceanography,
November 2017, Letnik:
62, Številka:
S1
Journal Article
Recenzirano
Odprti dostop
The potential for rivers to alter the flux of dissolved organic matter (DOM) from land to ocean is widely accepted. Yet anticipating when and where rivers behave as active reactors vs. passive pipes ...of DOM stands as a major knowledge gap in river biogeochemistry, resulting in uncertainties for global carbon models. Here, we investigate the controls on in-stream DOM dynamics by evaluating changes in DOM concentration and composition along several reaches of a medium-sized river network over one full hydrological year. Roughly half of the observations over time and space showed active reactor conditions and, among these, similar proportion of gains and losses was measured. High water residence times promoted the active over passive behavior of the reaches, while DOM properties and nitrate availability determined whether they supplied or removed DOM from the river. Among different DOM fractions, protein-like DOM both of terrestrial and aquatic origin seemed to drive bulk DOM patterns. Our study emphasizes the role of water residence time as a physical constraint for in-stream processes, and provides new insights into the key factors governing the net balance between in-stream gains and losses of DOM in rivers.
Increasing evidence exists that emerging pollutants such as pharmaceuticals (PhACs) and endocrine-disrupting compounds (EDCs) can be bioaccumulated by aquatic organisms. However, the relative role of ...trophic transfers in the acquisition of emerging pollutants by aquatic organisms remains largely unexplored. In freshwater ecosystems, wastewater treatment plants are a major source of PhACs and EDCs. Here we studied the entrance of emerging pollutants and their flow through riverine food webs in an effluent-influenced river. To this end we assembled a data set on the composition and concentrations of a broad spectrum of PhACs (25 compounds) and EDCs (12 compounds) in water, biofilm, and three aquatic macroinvertebrate taxa with different trophic positions and feeding strategies (Ancylus fluviatilis, Hydropsyche sp., Phagocata vitta). We tested for similarities in pollutant levels among these compartments, and we compared observed bioaccumulation factors (BAFs) to those predicted by a previously-developed empirical model based on octanol–water distribution coefficients (Dow). Despite a high variation in composition and levels of emerging pollutants across food web compartments, observed BAFs in Hydropsyche and Phagocata matched, on average, those already predicted. Three compounds (the anti-inflammatory drug diclofenac, the lipid regulator gemfibrozil, and the flame retardant TBEP) were detected in water, biofilm and (at least) one macroinvertebrate taxa. TBEP was the only compound present in all taxa and showed magnification across trophic levels. This suggests that prey consumption may be, in some cases, a significant exposure route. This study advances the notion that both waterborne exposure and trophic interactions need to be taken into account when assessing the potential ecological risks of emerging pollutants in aquatic ecosystems.
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•We studied emerging pollutants in an effluent-influenced river food web.•12 pharmaceuticals and 5 endocrine-disrupting compounds were detected.•Observed bioaccumulation factors matched those predicted by an empirical model.•The flame retardant TBEP experienced trophic magnification.•Both water and diet may be important sources of exposure for aquatic consumers.
Biofilms can be regarded as early warning systems for detection of the effects of toxicants on aquatic systems, because they have been successfully used for detection of other environmental stressors ...(e.g. pH, salinity, organic pollution). A variety of methods is used for detection of the effects of toxicants by use of biofilms. The methods range from structurally-based to functionally-based, and from in vitro-based to systemic approaches. Physiological approaches may be appropriate for detection of acute effects. Among these methods, photosynthesis is more related to the effect of toxicants affecting algal communities, directly or indirectly, and extracellular enzyme activity is less specific. Selecting one or the other may depend on the suspected direct effect of the toxicant. Integrated studies have revealed the relevance of toxicants to top-down or bottom-up regulation of the biofilm community. Persistent or chronic effects should affect other biofilm indicators, for example growth or biomass-related factors (e.g. chlorophyll), or community composition. Among these, community composition might better reflect the effects of the toxicant(s), because this may cause a shift from a sensitive to a progressively tolerant community. Community composition-based approaches do not usually adequately reflect cause-effect relationships and require complementary analysis of properties affected in the short-term, for example physiological properties. The current array of methods available must be wisely combined to disentangle the effects of chemicals on biofilms, and whether these effects are transient or persistent, to successfully translate the chemical action of toxicants into the effect they might have on the river ecosystem.
Impacts of environmental stressors on food webs are often difficult to predict because trophic levels can respond in divergent ways, and biotic interactions may dampen or amplify responses. Here we ...studied food‐web‐level impacts of urban wastewater pollution, a widespread source of degradation that can alter stream food webs via top‐down and bottom‐up processes. Wastewater may (1) subsidize primary producers by decreasing nutrient limitation, inducing a wide‐bottomed trophic pyramid. However, (2) wastewater may also reduce the quality and diversity of resources, which could decrease energy transfer efficiency by reducing consumer fitness, leading to predator starvation. Additionally, (3) if higher trophic levels are particularly sensitive to pollution, primary consumers could be released from predation pressure. We tested these hypotheses in 10 pairs of stream sites located upstream and downstream of urban wastewater effluents with different pollutant levels. We found that wastewater pollution reduced predator richness by ∼34%. Community size spectra (CSS) slopes were steeper downstream than upstream of wastewater effluents in all except one impact site where predators became locally extinct. Further, variation in downstream CSS slopes were correlated with pollution loads: the more polluted the stream, the steeper the CSS. We estimate that wastewater pollution decreased energy transfer efficiencies to primary consumers by ∼70%, limiting energy supply to predators. Additionally, traits increasing vulnerability to chemical pollution were overrepresented among predators, which presented compressed trophic niches (δ15N‐δ13C) downstream of effluents. Our results show that wastewater pollution can impact stream food webs via a combination of energy limitation to consumers and extirpation of pollution‐sensitive top predators. Understanding the indirect (biotically mediated) vs. direct (abiotic) mechanisms controlling responses to stress may help anticipating impacts of altered water quantity and quality, key signatures of global change.
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