Abstract
Environmental temperature is a critical factor for all forms of life, and thermal tolerance defines the habitats utilized by a species. Moreover, the evolutionary tuning of thermal ...perception can also play a key role in habitat selection. Yet, the relative importance of thermal tolerance and perception in environmental adaptation remains poorly understood. Thermal conditions experienced by anuran tadpoles differ among species due to the variation in breeding seasons and water environments selected by parental frogs. In the present study, heat tolerance and avoidance temperatures were compared in tadpoles from five anuran species that spatially and temporally inhabit different thermal niches. These two parameters were positively correlated with each other and were consistent with the thermal conditions of habitats. The species difference in avoidance temperature was 2.6 times larger than that in heat tolerance, suggesting the importance of heat avoidance responses in habitat selection. In addition, the avoidance temperature increased after warm acclimation, especially in the species frequently exposed to heat in their habitats. Characterization of the heat-sensing transient receptor potential ankyrin 1 (TRPA1) ion channel revealed an amphibian-specific alternatively spliced variant containing a single valine insertion relative to the canonical alternative spliced variant of TRPA1, and this novel variant altered the response to thermal stimuli. The two alternatively spliced variants of TRPA1 exhibited different thermal responses in a species-specific manner, which are likely to be associated with a difference in avoidance temperatures among species. Together, our findings suggest that the functional change in TRPA1 plays a crucial role in thermal adaptation processes.
ABSTRACT
We present an archival Atacama Large Millimeter/submillimeter Array (ALMA) study of the cyanide radical (CN) N = 1 − 0/carbon monoxide (CO) J = 1 − 0 intensity ratio in nearby (z < 0.05) ...ultra-luminous and luminous infrared galaxies (U/LIRGs). We identify 16 U/LIRGs that have been observed in both CN and CO lines at ∼500 pc resolution based on 16 different ALMA projects. We measure the (CN bright)/CO and (CN bright)/(CN faint) intensity ratios at an ensemble of molecular clouds scales (CN bright = CN N = 1 − 0, J = 3/2 − 1/2; CN faint = CN N = 1 − 0, J = 1/2 − 1/2 hyperfine groupings). Our global measured (CN bright)/CO ratios range 0.02–0.15 in LIRGs and 0.08–0.17 in ULIRGs. We attribute the larger spread in LIRGs to the variety of galaxy environments included in our sample. Overall, we find that the (CN bright)/CO ratio is higher in nuclear regions, where the physical and excitation conditions favour increased CN emission relative to the disc regions. 10 out of 11 galaxies which contain well-documented active galactic nuclei show higher ratios in the nucleus compared with the disc. Finally, we measure the median resolved (CN bright)/(CN faint) ratio and use it to estimate the total integrated CN line optical depth in ULIRGs (τ ∼ 0.96) and LIRGs (τ ∼ 0.23). The optical depth difference is likely due to the higher molecular gas surface densities found in the more compact ULIRG systems.
Ambient temperature fluctuations are detected via the thermosensory system which allows animals to seek preferable thermal conditions or escape from harmful temperatures. Evolutionary changes in ...thermal perception have thus potentially played crucial roles in niche selection. The genus Xenopus (clawed frog) is suitable for investigating the relationship between thermal perception and niche selection due to their diverse latitudinal and altitudinal distributions. Here we performed comparative analyses of the neuronal heat sensors TRPV1 and TRPA1 among closely related Xenopus species (X. borealis, X. muelleri, X. laevis, and X. tropicalis) to elucidate their functional evolution and to assess whether their functional differences correlate with thermal niche selection among the species. Comparison of TRPV1 among four extant Xenopus species and reconstruction of the ancestral TRPV1 revealed that TRPV1 responses to repeated heat stimulation were specifically altered in the lineage leading to X. tropicalis which inhabits warmer niches. Moreover, the thermal sensitivity of TRPA1 was lower in X. tropicalis than the other species, although the thermal sensitivity of TRPV1 and TRPA1 was not always lower in species that inhabit warmer niches than the species inhabit cooler niches. However, a clear correlation was found in species differences in TRPA1 activity. Heat‐evoked activity of TRPA1 in X. borealis and X. laevis, which are adapted to cooler niches, was significantly higher than in X. tropicalis and X. muelleri which are adapted to warmer niches. These findings suggest that the functional properties of heat sensors changed during Xenopus evolution, potentially altering the preferred temperature ranges among species.
We analyze the Sun's shadow observed with the Tibet-III air shower array and find that the shadow's center deviates northward (southward) from the optical solar disk center in the "away" ("toward") ...interplanetary magnetic field (IMF) sector. By comparing with numerical simulations based on the solar magnetic field model, we find that the average IMF strength in the away (toward) sector is 1.54±0.21_{stat}±0.20_{syst} (1.62±0.15_{stat}±0.22_{syst}) times larger than the model prediction. These demonstrate that the observed Sun's shadow is a useful tool for the quantitative evaluation of the average solar magnetic field.
When native cellulose is treated by catalytic oxidation with 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO)/NaBr/NaClO under aqueous conditions, significant amounts of carboxylate and aldehyde ...groups can be introduced on surfaces of cellulose I crystallites and into disordered regions without any changes in crystallinity of cellulose I or in the original fibrous morphology. In this study, behavior of ion exchange of carboxylate groups in the TEMPO-oxidized fibrous cellulose prepared from cotton linters was compared with that of fibrous carboxymethyl cellulose (F-CMC) with almost the same carboxylate content as that of the TEMPO-oxidized cellulose. These fibrous celluloses were soaked in various metal salt solutions, and metal ion contents in the celluloses were determined by X-ray fluorescence analysis after washing with water and drying of the celluloses. In all metal salts examined in the ion-exchange experiments, the TEMPO-oxidized cellulose had metal ion contents higher than those of F-CMC. Especially, lead, calcium and silver ions were greatly introduced into the TEMPO-oxidized cellulose with the metal ion/carboxylate molar ratio of about 1:1. The adsorption selectivity of metal ions on the TEMPO-oxidized cellulose was also studied using aqueous solutions containing multiple metal salts for soaking, and the following selectivity order was obtained:
Pb
2+>La
3+>Al
3+>Cu
2+>Ba
2+>Ni
2+>Co
2+>Cd
2+, Sr
2+, Mn
2+, Ca
2+>Mg
2+
Moreover, swelling ability of the TEMPO-oxidized celluloses having various metal carboxylate groups in water and wet tensile strength of handsheets prepared thereof were studied in terms of kinds of metal ion introduced. Degree of dissociation of the metal carboxylate groups in these celluloses is likely to affect the results of the above characteristics of the TEMPO-oxidized celluloses.
Between 2003 and 2007, 99 knees in 77 patients underwent opening wedge high tibial osteotomy. We evaluated the effect of initial stable fixation combined with an artificial bone substitute on the ...mid- to long-term outcome after medial opening-wedge high tibial osteotomy (HTO) for medial compartmental osteoarthritis or spontaneous osteonecrosis of the knee in 78 knees in 64 patients available for review at a minimum of five years (mean age 68 years; 49 to 82). The mean follow-up was 6.5 years (5 to 10). The mean Knee Society knee score and function score improved from 49.6 (SD 11.4, 26 to 72) and 56.6 (SD 15.6, 5 to 100) before surgery to 88.1 (SD 12.5, 14 to 100) and 89.4 (SD 15.6, 5 to 100) at final follow-up (p < 0.001) respectively. There were no significant differences between patients aged ≥ 70 and < 70 years. The mean standing femorotibial angle was corrected significantly from 181.7° (SD 2.7°, 175° to 185°) pre-operatively to 169.7° (SD 2.4°, 164° to 175°) at one year's follow-up (p < 0.001) and 169.6° (SD 3.0°, 157° to 179°) at the final follow-up (p = 0.69 vs one year). Opening-wedge HTO using a stable plate fixation system combined with a bone substitute is a reliable procedure that provides excellent results. Although this treatment might seem challenging for older patients, our results strongly suggest that the results are equally good.
Nociceptive receptors enable animals to sense tissue-damaging stimuli, thus playing crucial roles in survival. Due to evolutionary diversification, responses of nociceptive receptors to specific ...stimuli can vary among species. Multispecies functional comparisons of nociceptive receptors help elucidate their evolutionary process and molecular basis for activation. The transient receptor potential ankyrin 1 (TRPA1) ion channel serves as a nociceptive receptor for chemical and thermal stimuli that is heat-activated in reptiles and frogs while potentially cold-activated in rodents. Here, we characterized channel properties of avian TRPA1 in chicken. Chicken TRPA1 was activated by noxious chemicals that also activate TRPA1 in other vertebrates. Regarding thermal sensitivity, chicken TRPA1 was activated by heat stimulation, but not cold, thus thermal sensitivity of avian TRPA1 does not coincide with rodent TRPA1, although both are homeotherms. Furthermore, in chicken sensory neurons, TRPA1 was highly coexpressed with TRPV1, another nociceptive heat and chemical receptor, similar to mammals and frogs. These results suggest that TRPA1 acted as a noxious chemical and heat receptor, and was coexpressed with TRPV1 in the ancestral terrestrial vertebrate. The acquisition of TRPV1 as a novel heat receptor in the ancestral terrestrial vertebrate is likely to have affected the functional evolution of TRPA1 regarding thermal sensitivity and led to the diversification among diverse vertebrate species. Additionally, we found for the first time that chicken TRPA1 is activated by methyl anthranilate (MA) and its structurally related chemicals used as nonlethal bird repellents. MA-induced responses were abolished by a TRPA1 antagonist in somatosensory neurons, indicating that TRPA1 acts as a MA receptor in chicken. Furthermore, TRPA1 responses to MA varied among five diverse vertebrate species. Utilizing species diversity and mutagenesis experiments, three amino acids were identified as critical residues for MA-induced activation of chicken TRPA1.
The distribution of metals within a galaxy traces the baryon cycle and the buildup of galactic disks, but the detailed gas phase metallicity distribution remains poorly sampled. We have determined ...the gas phase oxygen abundances for 7138 H ii regions across the disks of eight nearby galaxies using Very Large Telescope/Multi Unit Spectroscopic Explorer (MUSE) optical integral field spectroscopy as part of the PHANGS-MUSE survey. After removing the first-order radial gradients present in each galaxy, we look at the statistics of the metallicity offset (ΔO/H) and explore azimuthal variations. Across each galaxy, we find low ( = 0.03-0.05 dex) scatter at any given radius, indicative of efficient mixing. We compare physical parameters for those H ii regions that are 1 outliers toward both enhanced and reduced abundances. Regions with enhanced abundances have high ionization parameter, higher H luminosity, lower H velocity dispersion, younger star clusters, and associated molecular gas clouds showing higher molecular gas densities. This indicates recent star formation has locally enriched the material. Regions with reduced abundances show increased H velocity dispersions, suggestive of mixing introducing more pristine material. We observe subtle azimuthal variations in half of the sample, but cannot always cleanly associate this with the spiral pattern. Regions with enhanced and reduced abundances are found distributed throughout the disk, and in half of our galaxies we can identify subsections of spiral arms with clearly associated metallicity gradients. This suggests spiral arms play a role in organizing and mixing the interstellar medium.
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