The microbiological study of maize rhizosphere (Zea x mays) cultivated in Brazil led to the isolation of a bacterium, which was identified as Rhizobium sp. based upon 16 S rRNA analysis. The chemical ...study of fractions obtained from bacterium extract allowed the identification of the compound N-(3-oxo-tetradec-7-enoyl)-homoserine lactone (1) using uni and bidimensional nuclear magnetic resonance spectroscopy (NMR), gas chromatography coupled to low resolution electron impact mass spectrometry (GC–MS), and ultra-high resolution Qq-time-of-flight mass spectrometry (UHR-QqTOF-MS). Biological assays with the strains Agrobacterium tumefasciens A136 and Chromobacterium violaceum CV026 were also performed and showed negative results, corroborating the hypothesis of production of only the long chain acyl homoserine lactone (AHL) 1, and the lack of presence of short or medium chain homologues, as observed by instrumental GC–MS and NMR analyses. The chemotaxonomic relevance of the presence of long chain AHL in Rhizobiaceae was discussed.
O objetivo é avaliar a frequência dos traumatismos em dentes decíduos, as sequelas advindas das injúrias envolvendo os tecidos de sustentação, bem como a associação destas injúrias às sequelas ...clínicas e radiográficas. Trata-se de um estudo clínico longitudinal, no qual foram avaliados 342 dentes traumatizados, atendidas no serviço odontológico de uma instituição pública, as avaliações foram realizadas após o traumatismo e decorrido 24 meses. Os dados foram submetidos a uma análise descritiva e ao teste Qui-quadrado (p≤0,05). A maior frequência de traumas acorreu na idade 24-35 meses, o principal fator etiológico à queda da própria altura (77,8 %), gênero masculino (59,5%), arco dentário o superior (96,5%), dente 51 (43,1%); 70,6% possuíam mais de um dente afetado e 8,2% histórico de trauma; a injúria mais prevalente foi a luxação lateral (33,6%). Na proservação, houve associação entre tipo de injúria ao tecido de suporte com a presença de sequelas radiográficas (p=0,02) e perda precoce (p=0,01). Conclui-se que as principais sequelas observadas foram a descoloração coronária e reabsorção radicular inflamatória e houve associação significante entre as variáveis tipo de injúria ao tecido de sustentação com a presença de sequelas radiográficas e a perda precoce do dente decíduo traumatizado.
El artículo presenta los resultados de una investigación dedicada a analizar las implicancias de las políticas públicas en la vida interna de las universidades argentinas, en línea con estudios ...internacionales elaborados en las últimas décadas. La hipótesis plantea que la reforma de los '90 propició la emergencia de nuevas áreas, funciones y roles en la gestión de las instituciones. Durante este período, la iniciativa estatal se orientó hacia la modernización de la gestión universitaria con el objetivo de aumentar sus niveles de eficacia y eficiencia. Los instrumentos de política pública estuvieron inspirados en el paradigma de la Nueva Gestión Pública (NGP), orientación que generó tensiones y nuevas vinculaciones con los tradicionales modelos de gobierno y gestión de las universidades nacionales. La estrategia de investigación combinó metodologías cuantitativas y cualitativas. Para ello, se recolectó y analizó información institucional, se aplicó una encuesta a una muestra de personal estratificada según períodos de creación y se realizaron entrevistas en profundidad. De acuerdo con los resultados obtenidos, las universidades han creado áreas de gestión para hacer frente a las demandas externas; a la vez que, generaron funciones y roles que son desempeñados por un nuevo tipo de profesionales que combina competencias académicas y administrativas
Kallikrein-related peptidases are secreted serine proteases that exert stimulatory or inhibitory effects on tumor progression. A recent study demonstrated that kallikrein-related peptidase 5 (KLK5) ...concentration is elevated in serum of patients with ovarian carcinoma. At the moment, the presence of KLK5 in other ovarian pathological lesions is not clearly determined. Moreover, the possibility of a spontaneous humoral immune response to KLK5 has not been studied yet.
In this study, we examined KLK5 levels and antibody (IgG and IgM) response to KLK5 in the serum of 50 healthy women, 50 patients with benign pelvic masses, 17 patients with ovarian borderline tumors, and 50 patients with ovarian carcinomas, using 3 enzyme-linked immunosorbent assay tests available in-house.
At 95% specificity on healthy controls, 52% of patients with ovarian carcinoma showed high serum KLK5 (sKLK5) levels, whereas patients with benign pathological lesions or borderline tumors showed almost undetectable sKLK5 levels. Moreover, sKLK5 levels were positively associated to International Federation of Gynaecologists and Obstetricians stage suggesting a possible role of sKLK5 in ovarian cancer progression. Our results about humoral response showed elevated levels of KLK5-specific antibodies in 20% of patients with benign masses, 26% of patients with borderline tumors, and 36% of patients with ovarian carcinomas when compared with healthy controls. Interestingly, KLK5 antibodies were also found in patients with undetectable sKLK5 levels.
In conclusion, our results showed that KLK5 is a potential new biomarker to be used in combination with other biomarkers for ovarian cancer detection. Moreover, the existence of KLK5 antibodies suggests that KLK5 might represent a possible target for immune-based therapies.
Graph Neural Networks (GNNs) have become the leading paradigm for learning on (static) graph-structured data. However, many real-world systems are dynamic in nature, since the graph and node/edge ...attributes change over time. In recent years, GNN-based models for temporal graphs have emerged as a promising area of research to extend the capabilities of GNNs. In this work, we provide the first comprehensive overview of the current state-of-the-art of temporal GNN, introducing a rigorous formalization of learning settings and tasks and a novel taxonomy categorizing existing approaches in terms of how the temporal aspect is represented and processed. We conclude the survey with a discussion of the most relevant open challenges for the field, from both research and application perspectives.
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