17β-estradiol (E2) may interfere with endocrine, metabolic, and gender-differentiated functions in liver in both females and males. Indirect mechanisms play a crucial role because of the E2 influence ...on the pituitary GH secretion and the GHR-JAK2-STAT5 signaling pathway in the target tissues. E2, through its interaction with the estrogen receptor, exerts direct effects on liver. Hypothyroidism also affects endocrine and metabolic functions of the liver, rendering a metabolic phenotype with features that mimic deficiencies in E2 or GH. In this work, we combined the lipid and transcriptomic analysis to obtain comprehensive information on the molecular mechanisms of E2 effects, alone and in combination with GH, to regulate liver functions in males. We used the adult hypothyroid-orchidectomized rat model to minimize the influence of internal hormones on E2 treatment and to explore its role in male-differentiated functions. E2 influenced genes involved in metabolism of lipids and endo-xenobiotics, and the GH-regulated endocrine, metabolic, immune, and male-specific responses. E2 induced a female-pattern of gene expression and inhibited GH-regulated STAT5b targeted genes. E2 did not prevent the inhibitory effects of GH on urea and amino acid metabolism-related genes. The combination of E2 and GH decreased transcriptional immune responses. E2 decreased the hepatic content of saturated fatty acids and induced a transcriptional program that seems to be mediated by the activation of PPARα. In contrast, GH inhibited fatty acid oxidation. Both E2 and GH replacements reduced hepatic CHO levels and increased the formation of cholesterol esters and triacylglycerols. Notably, the hepatic lipid profiles were endowed with singular fingerprints that may be used to segregate the effects of different hormonal replacements. In summary, we provide in vivo evidence that E2 has a significant impact on lipid content and transcriptome in male liver and that E2 exerts a marked influence on GH physiology, with implications in human therapy.
Thyroid hormones are required for normal growth and development in mammals. Congenital-neonatal hypothyroidism (CH) has a profound impact on physiology, but its specific influence in liver is less ...understood. Here, we studied how CH influences the liver gene expression program in adulthood. Pregnant rats were given the antithyroid drug methimazole (MMI) from GD12 until PND30 to induce CH in male offspring. Growth defects due to CH were evident as reductions in body weight and tail length from the second week of life. Once the MMI treatment was discontinued, the feed efficiency increased in CH, and this was accompanied by significant catch-up growth. On PND80, significant reductions in body mass, tail length, and circulating IGF-I levels remained in CH rats. Conversely, the mRNA levels of known GH target genes were significantly upregulated. The serum levels of thyroid hormones, cholesterol, and triglycerides showed no significant differences. In contrast, CH rats showed significant changes in the expression of hepatic genes involved in lipid metabolism, including an increased transcription of PPARα and a reduced expression of genes involved in fatty acid and cholesterol uptake, cellular sterol efflux, triglyceride assembly, bile acid synthesis, and lipogenesis. These changes were associated with a decrease of intrahepatic lipids. Finally, CH rats responded to the onset of hypothyroidism in adulthood with a reduction of serum fatty acids and hepatic cholesteryl esters and to T3 replacement with an enhanced activation of malic enzyme. In summary, we provide in vivo evidence that neonatal hypothyroidism influences the hepatic transcriptional program and tissue sensitivity to hormone treatment in adulthood. This highlights the critical role that a euthyroid state during development plays on normal liver physiology in adulthood.
Introduction:
Several studies have shown increased occurrence of migraine in ADHD patients. However, there is less evidence on whether migraine patients also have a higher ADHD frequency. The aim of ...this paper is determining whether the prevalence of ADHD symptoms or impulsivity is higher in patients with episodic migraine.
Methods:
An observational cohort study has been conducted. Patients with episodic migraine were included. The ADHD Rating scale, the Adult ADHD Self-Report Scale, and the scale of impulsiveness of Plutchik were used.
Results:
The mean value of inattention, hyperactivity and impulsivity scores on the ADHD scale was 5 ± 3.8 in cases and 2.7 ± 2.2 in controls (p < .00001), 4 ± 3.2 in cases and 2.5 ± 2.4 in controls (p = .000621) and 2 ± 1.5 in cases and 1.1 ± 1 in controls (p = .000407), respectively.
Conclusion:
Adults with migraine have a higher prevalence of ADHD symptoms. This should be considered when assessing these patients.
ABSTRACT
Hepatic steatosis is a prominent feature in patients with growth hormone (GH) deficiency. The ubiquitin ligase SOCS2 attenuates hepatic GH signaling by inhibiting the Janus kinase 2 ...(JAK2)‐signal transducer and activator of transcription 5b (STAT5b) axis. Here, we investigated the role of SOCS2 in the development of diet‐induced hepatic steatosis and insulin resistance. SOCS2‐knockout (SOCS2−/−) mice and wild‐type littermates were fed for 4 mo with control or high‐fat diet, followed by assessment of insulin sensitivity, hepatic lipid content, and expression of inflammatory cytokines. SOCS2−/− mice exhibited increased hepatic TG secretion by 77.6% (P< 0.001) as compared with wild‐type control mice and were protected from high‐fat‐diet (HFD)‐induced hepatic steatosis, showing 49.3% (P<0.01) reduction in liver TG levels compared to HFD‐fed wild‐type littermates. In contrast, we found that HFD‐triggered attenuation of systemic insulin sensitivity was more marked in SOCS2−/− mice. Livers from the HFD‐fed SOCS2−/− mice showed increased NF‐κB activity as well as elevated expression of genes for the inflammatory cytokines IFN‐γ and IL‐6. An inhibitory role of SOCS2 on Toll‐like receptor 4 signaling was demonstrated in macrophages obtained from the SOCS2−/− and wild‐type mice. This study identified SOCS2 as an important regulator of hepatic homeostasis under conditions of high‐fat dietary stress.—Zadjali, F., Santana‐Farre, R., Vesterlund, M., Carow, B., Mirecki‐Garrido, M., Hernandez‐Hernandez, I., Flodström‐Tullberg, M., Parini, P., Rottenberg, M., Norstedt, G., Fernandez‐Perez, L., Flores‐Morales, A. SOCS2 deletion protects against hepatic steatosis but worsens insulin resistance in high‐fat‐diet‐fed mice. FASEB J. 26, 3282–3291 (2012). www.fasebj.org
Abstract only
SOCS proteins act as negative regulators of GH signalling. Recently, E2 was demonstrated to induce SOCS‐2 synthesis and inhibit GH signalling in liver and kidney cells. To evaluate the ...role of E2 on GH‐regulated SOCS in vivo, this work used orchidectomized‐hypothyroid (TX‐OX) male rats to minimize the influence of internal hormones on treatment. OX‐TX rats were treated 3 weeks with vehicle or E2 before hormonal replacement with GH, T3, or T3 plus GH during 7 days. We analyzed body growth, serum lipids and steady‐state levels of SOCS‐2, ‐3, and CIS mRNA expression in liver in relation to cited hormones. Hypothyroidism resulted in an over 2‐fold increase in serum cholesterol (Cho) levels and 2‐fold reduction in serum triglyceride (Tg) levels. T3 treatment decreased Cho and GH treatment resulted in 3‐4‐fold reduction in serum FFAs levels. Hypothyroidism reduced the levels of SOCS‐2 and CIS mRNA and induced SOCS‐3. E2 reversed the effects of hypothyroidism on SOCS2 and CIS but not on SOCS3 mRNA levels. Unlike T3, the effects of hypothyroidism on SOCS2 and CIS mRNA levels were reversed by GH. T3 reversed the effects of hypothyroidism on SOCS3. Whereas E2 enhanced the effect of T3 on SOCS2 and CIS, T3 effect on SOCS3 was antagonized. These results indicate that SOCS are differently regulated in vivo and an important physiological role for E2, GH and T3 in maintaining levels of SOCS in male liver MEC‐SAF2006‐07824‐ and Pfizer Spain.
Liver X receptor (LXR) agonists have been shown to influence the development of hyperlipidemia and atherosclerosis in mouse models. It has also been demonstrated that some LXR agonists can cause ...hepatic steatosis in experimental animals. Growth hormone (GH) is known to regulate hepatic metabolism and the absence of hepatic GH receptors (GHR) leads to hepatic steatosis. In this study, we analyzed whether the actions of LXR agonists could involve interference with GH signaling. We showed that LXR agonists impair GH signaling in hepatocytes. LXR agonist treatment attenuated GH induction of suppressor of cytokine signaling 2 (SOCS2), SOCS3, and CIS mRNA levels in BRL-4 cells. Likewise, the activity of a luciferase reporter vector driven by the GH response element (GHRE) of the SOCS2 gene was inhibited by simultaneous treatment with an LXR agonist. The inhibitory effect of LXR agonists on GH signals can be mimicked by overexpression of the LXR regulated factors, sterol regulatory element binding protein 1 (SREBP1) and SREBP2, in hepatic cells. In both cases total and phosphorylated signal transducers and activators of transcription 5b (STAT5b) protein levels were significantly reduced. DNA binding assays demonstrated that SREBP1 binds to an E-box within a previously defined GHRE in the SOCS2 gene promoter, but does not compete with STAT5b binding to a nearby site in the same promoter construct. Taken together, our findings indicate that the inhibitory effects of LXR agonists on GH signaling are mediated by SREBP1, through the downregulation of STAT5b gene transcription and stimulation of STAT5b protein degradation. The findings provide a new insight into the understanding of the molecular actions of LXR agonists, which may be of relevance to their pharmacological actions.
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