Young children are an important focus of sun-protection efforts, but there has been relatively little study of sun-protection interventions developed for preschool-aged children and their parents. ...This paper reports on the evaluation of
Sun Protection is Fun! (S.P.F.), designed to improve parents' practices and psychosocial outcomes related to protecting preschool children from sun exposure.
A group-randomized trial was conducted in 20 preschools to evaluate the
S.P.F. parent intervention that included a video, newsletters, and handbooks. A separate, on-site intervention for preschool staff aimed to create a preschool climate that encouraged parents' sun protection for their children. Cross-sectional samples of parents completed surveys at baseline (
n = 384), 12 months (
n = 640), and 24 months (
n = 694).
S.P.F. demonstrated significant effects on parents' sun-avoidance strategies at 12 months (
P < .05) and sunscreen use at 24 months (
P < .05). There were significant intervention effects on parents' sun-protection knowledge (
P < .001), perceived norms of teachers' sunscreen use (
P < .001), sunscreen impediments (
P < .05), and sunscreen expectancies (
P < .05) at 12 months. Parents' perceived norms of teacher sunscreen use were significantly improved at 24 months (
P < .001).
More intense intervention strategies may need to complement take-home materials to result in greater effects on parents' sun protection for their children.
Little is known about platelet transfusions in pediatric critical illness. We sought to describe the epidemiology, indications, and outcomes of platelet transfusions among critically ill children.
...Prospective cohort study.
Multicenter (82 PICUs), international (16 countries) from September 2016 to April 2017.
Children ages 3 days to 16 years prescribed a platelet transfusion in the ICU during screening days.
None.
Over 6 weeks, 16,934 patients were eligible, and 559 received at least one platelet transfusion (prevalence, 3.3%). The indications for transfusion included prophylaxis (67%), minor bleeding (21%), and major bleeding (12%). Thirty-four percent of prophylactic platelet transfusions were prescribed when the platelet count was greater than or equal to 50 × 10 cells/L. The median (interquartile range) change in platelet count post transfusion was 48 × 10 cells/L (17-82 × 10 cells/L) for major bleeding, 42 × 10 cells/L (16-80 × 10 cells/L) for prophylactic transfusions to meet a defined threshold, 38 × 10 cells/L (17-72 × 10 cells/L) for minor bleeding, and 25 × 10 cells/L (10-47 × 10 cells/L) for prophylaxis in patients at risk of bleeding from a device. Overall ICU mortality was 25% but varied from 18% to 35% based on indication for transfusion. Upon adjusted analysis, total administered platelet dose was independently associated with increased ICU mortality (odds ratio for each additional 1 mL/kg platelets transfused, 1.002; 95% CI, 1.001-1.003; p = 0.005).
The majority of platelet transfusions are given as prophylaxis to nonbleeding children, and significant variation in platelet thresholds exists. Studies are needed to clarify appropriate indications, with focus on prophylactic transfusions.
Prostate movement imposes limits on safe dose-escalation with external beam radiation therapy. If the precise daily location of the prostate is known, dose escalation becomes more feasible. We have ...developed an approach to dose escalation using a combination of prostate brachytherapy followed by external beam radiation therapy in which fiducial markers are placed along with125 I seeds during transperineal interstitial permanent prostate brachytherapy. These markers serve to verify daily prostate location during the subsequent external beam radiotherapy. Prior to implementing this approach, preliminary studies were performed to test visibility of the markers. Three different125 I seed models, as well as gold and silver marker seeds were placed within tissue-equivalent phantoms. Images were obtained with conventional x-rays (75–85 kV) and 6 MV photons from a linear accelerator. All125 I seed models were clearly visible on conventional x-rays but none were seen with 6 MV photons. The gold markers were visible with both energies. The silver markers were visible with conventional x-rays and 6 MV x-rays, but not as clearly as the gold seeds at 6 MV. Subsequently, conventional x-rays, CT scans, and 6 MV port films were obtained in 29 patients in whom fiducial gold marker seeds were implanted into the prostate during125 I prostate brachytherapy. To address the possibility of “seed migration” within the prostate, CT scans were repeated 5 weeks apart in 14 patients and relative positions of the gold seeds were evaluated. The repeated CT scans showed no change in intraprostatic gold marker location, suggesting minimal migration. The gold seeds were visible with conventional x-rays, CT, and 6 MV port films in all patients. During the course of external beam radiation therapy, the gold markers were visible on routine 6 MV port films and were seen in different locations from film to film suggesting prostate motion. Mean daily displacement was 4–5 mm in the anterior-posterior, and 4–5 mm in superior-inferior dimensions. Left-right displacement appeared less, averaging 2–3 mm. We conclude that implantation of gold marker seeds during prostate brachytherapy represents an easily implemented and practical means of prostate localization during subsequent image-guided external beam radiotherapy. With such markers, conformality of the external beam component can be confidently improved without expensive new equipment.
Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of ...conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-mining caterpillars used by more than 5000 species of parasitoids. Several hundred thousand specimens of ACG-reared adult Lepidoptera and parasitoids have been intensively and extensively studied morphologically by many taxonomists, including most of the co-authors. DNA barcoding -- the use of a standardized short mitochondrial DNA sequence to identify specimens and flush out undisclosed species -- was added to the taxonomic identification process in 2003. Barcoding has been found to be extremely accurate during the identification of about 100 000 specimens of about 3500 morphologically defined species of adult moths, butterflies, tachinid flies, and parasitoid wasps. Less than 1% of the species have such similar barcodes that a molecularly based taxonomic identification is impossible. No specimen with a full barcode was misidentified when its barcode was compared with the barcode library. Also as expected from early trials, barcoding a series from all morphologically defined species, and correlating the morphological, ecological and barcode traits, has revealed many hundreds of overlooked presumptive species. Many but not all of these cryptic species can now be distinguished by subtle morphological and/or ecological traits previously ascribed to 'variation' or thought to be insignificant for species-level recognition. Adding DNA barcoding to the inventory has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.
We investigate the correlation between far-infrared (FIR) and radio luminosities in distant galaxies, a lynchpin of modern astronomy. We use data from the Balloon-borne Large Aperture Submillimetre ...Telescope (BLAST), Spitzer, the Large Apex BOlometer CamerA (LABOCA), the Very Large Array and the Giant Metre-wave Radio Telescope (GMRT) in the Extended Chandra Deep Field South (ECDFS). For a catalogue of BLAST 250-μm-selected galaxies, we remeasure the 70–870-μm flux densities at the positions of their most likely 24-μm counterparts, which have a median interquartile redshift of 0.74 0.25, 1.57. From these, we determine the monochromatic flux density ratio, q250(= log10S250 μm/S1400 MHz), and the bolometric equivalent, qIR. At z≈ 0.6, where our 250-μm filter probes rest-frame 160-μm emission, we find no evolution relative to q160 for local galaxies. We also stack the FIR and submm images at the positions of 24-μm- and radio-selected galaxies. The difference between qIR seen for 250-μm- and radio-selected galaxies suggests that star formation provides most of the IR luminosity in ≲100-μJy radio galaxies, but rather less for those in the mJy regime. For the 24-μm sample, the radio spectral index is constant across 0 < z < 3, but qIR exhibits tentative evidence of a steady decline such that qIR∝ (1 +z)−0.15±0.03– significant evolution, spanning the epoch of galaxy formation, with major implications for techniques that rely on the FIR/radio correlation. We compare with model predictions and speculate that we may be seeing the increase in radio activity that gives rise to the radio background.
Abstract only
As part of the NIH SPARC program efforts to study the intrinsic cardiac nervous system, we developed the first comprehensive molecular phenotyping data on the right atrial ganglionated ...plexus (RAGP) within the intrinsic cardiac nervous system of the pig. We collected hundreds of single neurons from the RAGP and assayed these for expression of a wide range of genes relevant to neuronal functions. The sinoatrial node (SAN) of 4 pigs were injected with FastBlue, a retrograde tracer, labeling neurons projecting to the SAN. The RAGP of those pigs were then collected and sectioned. Both SAN‐projecting and non‐SAN‐projecting single neurons in RAGP were collected through laser capture microdissection (LCM), allowing for spatial mapping of all collected samples. Over 400 single neurons were collected (n=4 animals) and each neuron was assayed for over 300 genes using high throughput microfluidic qPCR using BioMark. Neuronal phenotypes were distinguished by multivariate analysis showing patterns of network activity between multiple genes. Interestingly, these phenotypes show no spatial preferences within the RAGP. Our results revealed extensive combinatorial expression of neurotransmitters across the RAGP neuronal phenotypes. Additionally, there was a large overlap in expression profiles of SAN‐projecting and non‐SAN‐projecting neurons, without any single gene or module acting as a distinguishing marker between the connectionally labeled groups. Previous studies have focused on cholinergic and catecholaminergic processes, showing evidence for extensive protein expression of cholinergic markers in RAGP. Our findings are in stark contrast to these results and demonstrate high gene expression correlation between cholinergic and catecholaminergic markers such as tyrosine hydroxylase (Th) and choline acetyltransferase (ChAT) in single neurons. This finding suggests that cells are poised for a rapid shift in neurotransmitter signaling as well as the possibility for post transcriptional regulation of neuron phenotype plasticity. Our present findings significantly expand the list of neuromodulatory peptides and their receptors known to be expressed in RAGP. For example, neuropeptide Y and somatostatin showed distinct co‐expression patterns with their respective receptors with significant non‐overlapping expression of transmitters and cognate receptors across single neurons. Taken together, our results reveal a complex organization of neuronal networks within RAGP‐SAN complex that is not entirely governed by connectivity or spatial location, with combinatorial patterns of local paracrine networks with potential influence on cardiac function.
Support or Funding Information
Supported by the NIH Common Fund SPARC Program award OT2 OD023848 and NIH NHLBI Multiscale Modeling award U01 HL133360.
Elevated plasma homocysteine is associated with a variety of diseases in humans including coronary heart disease, stroke, peripheral vascular disease, and birth defects. However, the mechanism by ...which plasma homocysteine affects cells is unknown. We have examined the growth of isogenic wild-type and cystathionine β-synthase (CBS) deficient yeast in response to homocysteine and its immediate metabolic precursor,
S-adenosylhomocysteine (SAH). CBS deficient yeast export significantly more homocysteine into the media than wild-type yeast and have elevated internal pools of homocysteine and SAH. We found that 5 mM homocysteine added to the media had very little effect on the growth of wild-type or CBS deficient yeast, although intracellular homocysteine concentrations increased five- to tenfold. In contrast, as little as
25
μM
S-adenosylhomocysteine inhibited the growth of CBS deficient yeast, but had no effect on wild-type yeast. Measurements of the intracellular
S-adenosylmethionine (SAM) and SAH indicate that CBS deficient yeast contain reduced SAM/SAH ratios relative to wild-type, and this ratio is further reduced by adding SAH to the media. Growth inhibition by SAH in CBS deficient yeast can be totally reversed by addition of SAM to the media, indicating that the ratio and not absolute level is critical for cell growth. These results suggest that CBS plays a key role in the regulation of the SAM/SAH ratio inside cells and that excessive perturbations of this ratio can inhibit growth. We hypothesize that elevated extracellular homocysteine present in humans may reflect an altered intracellular SAM/SAH ratio and that this may be related to disease pathogenesis.
The conserved domain database (CDD) is part of NCBI's Entrez database system and serves as a primary resource for the annotation of conserved domain footprints on protein sequences in Entrez. ...Entrez's global query interface can be accessed at http://www.ncbi.nlm.nih.gov/Entrez and will search CDD and many other databases. Domain annotation for proteins in Entrez has been pre-computed and is readily available in the form of 'Conserved Domain' links. Novel protein sequences can be scanned against CDD using the CD-Search service; this service searches databases of CDD-derived profile models with protein sequence queries using BLAST heuristics, at http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi. Protein query sequences submitted to NCBI's protein BLAST search service are scanned for conserved domain signatures by default. The CDD collection contains models imported from Pfam, SMART and COG, as well as domain models curated at NCBI. NCBI curated models are organized into hierarchies of domains related by common descent. Here we report on the status of the curation effort and present a novel helper application, CDTree, which enables users of the CDD resource to examine curated hierarchies. More importantly, CDD and CDTree used in concert, serve as a powerful tool in protein classification, as they allow users to analyze protein sequences in the context of domain family hierarchies.
Gallium nitride (GaN) high electron mobility transistors (HEMTs) are key components of modern radio frequency (RF) power amplifiers. However, device self-heating negatively impacts both the ...performance and reliability of GaN HEMTs. Accordingly, laser-based pump-probe methods have been used to characterize the thermal resistance network of epitaxial material stacks that are used to fabricate HEMT structures. However, validation studies of these measurement results at the device level are lacking. In the present work, a GaN-on-SiC wafer was characterized using frequency-domain thermoreflectance and steady-state thermoreflectance techniques. The thermal conductivity of the GaN channel/buffer layer, SiC substrate, and the interfacial thermal boundary resistance at the GaN/SiC interface were determined. Results were validated by performing thermal imaging and modeling of a transmission line measurement (TLM) structure fabricated on the GaN-on-SiC wafer.
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