Gut microbiota has been suggested to affect lipid metabolism. The objective of this study was to characterize the faecal microbiota signature and both short chain fatty acids (SCFAs) and bile acids ...(BA) profile of hypercholesterolemic subjects. Microbiota composition, SCFAs, BA and blood lipid profile from male volunteers with hypercholesterolemia (HC) and normocholesterolemia (NC) were determined by 16S rDNA sequencing, HPLC, GC and NMR, respectively. HC subjects were characterized by having lower relative abundance of Anaeroplasma (0.002% vs 0.219%, p-value = 0.026) and Haemophilus (0.041% vs 0.078%, p-value = 0.049), and higher of Odoribacter (0.51% vs 0.16%; p-value = 0.044). Correlation analysis revealed that Anaeroplasma and Haemophilus were associated to an unfavourable lipid profile: they correlated negatively to cholesterol and triglycerides related biomarkers and the ratio total to high density lipoprotein (HDL) cholesterol, and positively to HDL size. Odoribacter displayed an opposite behaviour. Faecal SCFAs profile revealed higher abundance of isobutyric (2.76% vs 0.82%, p-value = 0.049) and isovaleric acid (1.32% vs 0.06%, p-value = 0.016) in HC. Isobutyric acid correlated positively with Odoribacter and lipid parameters indicative of an unfavourable profile. BA profile did not show differences between groups. It was concluded that HC subjects showed a particular faecal bacterial signature and SCFAs profile associated with their lipid profile.
Sandcastle worms have developed protein‐based adhesives, which they use to construct protective tubes from sand grains and shell bits. A key element in the adhesive delivery is the formation of a ...fluidic complex coacervate phase. After delivery, the adhesive transforms into a solid upon an external trigger. In this work, a fully synthetic in situ setting adhesive based on complex coacervation is reported by mimicking the main features of the sandcastle worm's glue. The adhesive consists of oppositely charged polyelectrolytes grafted with thermoresponsive poly(N‐isopropylacrylamide) (PNIPAM) chains and starts out as a fluid complex coacervate that can be injected at room temperature. Upon increasing the temperature above the lower critical solution temperature of PNIPAM, the complex coacervate transitions into a nonflowing hydrogel while preserving its volume—the water content in the material stays constant. The adhesive functions in the presence of water and bonds to different surfaces regardless of their charge. This type of adhesive avoids many of the problems of current underwater adhesives and may be useful to bond biological tissues.
A fully synthetic in situ setting adhesive based on complex coacervation is reported by mimicking the main features of the sandcastle worm's glue. The adhesive consists of oppositely charged polyelectrolytes grafted with thermoresponsive poly(N‐isopropylacrylamide) chains. The adhesive starts out as an injectable fluid at room temperature. Upon increasing the temperature, the complex coacervate transitions into a nonflowing hydrogel which bonds to different surfaces.
Analyses of Arabidopsis thaliana defense response to the damping-off oomycete pathogen Pythium irregulare show that resistance to P. irregulare requires a multicomponent defense strategy. Penetration ...represents a first layer, as indicated by the susceptibility of pen2 mutants, followed by recognition, likely mediated by ERECTA receptor-like kinases. Subsequent signaling of inducible defenses is predominantly mediated by jasmonic acid (JA), with insensitive coi1 mutants showing extreme susceptibility. In contrast with the generally accepted roles of ethylene and salicylic acid cooperating with or antagonizing, respectively, JA in the activation of defenses against necrotrophs, both are required to prevent disease progression, although much less so than JA. Meta-analysis of transcriptome profiles confirmed the predominant role of JA in activation of P. irregulare-induced defenses and uncovered abscisic acid (ABA) as an important regulator of defense gene expression. Analysis of cis-regulatory sequences also revealed an unexpected overrepresentation of ABA response elements in promoters of P. irregulare-responsive genes. Subsequent infections of ABA-related and callose-deficient mutants confirmed the importance of ABA in defense, acting partly through an undescribed mechanism. The results support a model for ABA affecting JA biosynthesis in the activation of defenses against this oomycete.
Cis‐(+)‐12‐oxo‐phytodienoic acid (OPDA) is likely to play signaling roles in plant defense that do not depend on its further conversion to the phytohormone jasmonic acid. To elucidate the role of ...OPDA in Solanum lycopersicum (tomato) plant defense, we have silenced the 12‐oxophytodienoate reductase 3 (OPR3) gene. Two independent transgenic tomato lines (SiOPR3‐1 and SiOPR3‐2) showed significantly reduced OPR3 expression upon infection with the necrotrophic pathogen Botrytis cinerea. Moreover, SiOPR3 plants are more susceptible to this pathogen, and this susceptibility is accompanied by a significant decrease in OPDA levels and by the production of JA‐Ile being almost abolished. OPR3 silencing also leads to a major reduction in the expression of other genes of the jasmonic acid (JA) synthesis and signaling pathways after infection. These results confirm that in tomato plants, as in Arabidopsis, OPR3 determines OPDA availability for JA biosynthesis. In addition, we show that an intact JA biosynthetic pathway is required for proper callose deposition, as its pathogen‐induced accumulation is reduced in SiOPR3 plants. Interestingly, OPDA, but not JA, treatment restored basal resistance to B. cinerea and induced callose deposition in SiOPR3‐1 and SiOPR3‐2 transgenic plants. These results provide clear evidence that OPDA by itself plays a major role in the basal defense of tomato plants against this necrotrophic pathogen.
The diesel engine requirements regarding the reduction of exhaust emissions, especially nitrogen oxide (NOx) and particulate matter (PM) are becoming more stringent year by year. A current method ...used for NOx control is exhaust gas recirculation (EGR). However, this approach significantly increases the production of soot for medium and high rates. Water addition can also be used for NOx control, achieving 50% NOx reduction rates in relation to EGR with a lower production of soot and without additional carbon monoxide (CO) and hydrocarbons (HC) substantially. This paper analyses the weaknesses and the strengths of adding water into the intake manifold with a flow-blurring injector for NOx reduction on a current technology diesel engine with multiple injection thorough a proprietary tool for heat release rate that considered real gas properties. A reduction of NOx emissions around 60–70% was achieved with water injection at different loads and speeds. Besides, a clear relationship was established between the minimum attainable NOx emission and the thermal capacity of the load (air plus water), this result stablish the hard relationship between NOx formation and combustion chamber temperature and therefore shows the strong dependence of the Zeldovich mechanism.
•Effects of adding water into the intake manifold with a flow-blurring injector.•NOx reduction on a current technology diesel engine with multiple injection.•The NOx emission was reduced by adding water in comparison to the EGR.•A reduction of NOx emissions around 60–70% was achieved with water injection.•The minimum attainable NOx emission and the thermal capacity are related.
Satellite-derived sea ice concentration (SIC) and reanalyzed atmospheric data are used to explore the predictability of the winter Euro-Atlantic climate resulting from autumn SIC variability over the ...Barents–Kara Seas region (SIC/BK). The period of study is 1979/80–2012/13. Maximum covariance analyses show that the leading predictand is indistinguishable from the North Atlantic Oscillation (NAO). The leading covariability mode between September SIC/BK and winter North Atlantic–European sea level pressure (SLP) is not significant, indicating that no empirical prediction skill can be achieved. The leading covariability mode with either October or November SIC/BK is moderately significant (significance levels <10%), and both predictor fields yield a cross-validated NAO correlation of 0.3, suggesting some empirical prediction skill of the winter NAO index, with sea ice reduction in the Barents–Kara Seas being accompanied by a negative NAO phase in winter. However, only November SIC/BK provides significant cross-validated skill of winter SLP, surface air temperature, and precipitation anomalies over the Euro-Atlantic sector, namely in southwestern Europe. Statistical analysis suggests that November SIC/BK anomalies are associated with a Rossby wave train–like anomaly across Eurasia that affects vertical wave activity modulating the stratospheric vortex strength, which is then followed by downward propagation of anomalies that impact transient-eddy activity in the upper troposphere, helping to settle and maintain the NAO-like pattern at surface. This stratospheric pathway is not detected when using October SIC/BK anomalies. Hence, only November SIC/BK, with a one-month lead time, could be considered as a potential source of regional predictability.
Introduction
On March 11th, 2020, the WHO declared the SARS‐Cov‐2 pandemic. Syndromes have been detected in relation to COVID‐19 such as encephalitis, acute necrotizing hemorrhagic encephalopathy and ...cerebrovascular complications. There are also cases of peripheral nervous system involvement.
Methods
Our case would be the 3rd patient with MFS associated with COVID‐19 as far as we know.
Results
We present a 51 years old female diagnosed with MFS two weeks after COVID‐19. RTPCR to SARS‐CoV‐2 was negative but IgG was positive.
Conclusion
Most of the cases were mild or moderate with typical signs and symptoms. All were treated with IV immunoglobulin with good response in most cases. Despite the short evolution time of the cases surviving the current pandemic, the description of cases of post‐infectious neurological syndromes suggests that this is probably not an infrequent complication in the subacute stage of Covid‐19 disease.
The current European F-gas regulation establishes restrictions in the use of fluoride refrigerants with a high global warming potential (GWP) in applications of refrigeration and air conditioning ...(RAC) systems. Moreover, a gradual limitation on the GWP weight of the fluoride refrigerants placed on the market is ongoing and will end with approximately one-fifth of today's offer. In this context, many of the RAC systems operate with refrigerants R134a, R404A, and R410A that have GWP values of 1300, 3943 and 2088, respectively, are being forced to be replaced by environmentally friendly alternatives, like hydrofluoroolefin (HFO) refrigerants and their mixtures with hydrofluorocarbons (HFC), which can be designed to present intermediate characteristics and become the ideal candidate many RAC applications. This work presents the most recent HFC/HFO/HC/R744 refrigerant mixture options for an alternative to the refrigerants mentiond above and compares their energetic and performance with the early developed mixture prototypes.
Climate models are seen by many to be unverifiable. However, near-term climate predictions up to 10 years into the future carried out recently with these models can be rigorously verified against ...observations. Near-term climate prediction is a new information tool for the climate adaptation and service communities, which often make decisions on near-term time scales, and for which the most basic information is unfortunately very scarce. The Fifth Coupled Model Intercomparison Project set of co-ordinated climate-model experiments includes a set of near-term predictions in which several modelling groups participated and whose forecast quality we illustrate here. We show that climate forecast systems have skill in predicting the Earth's temperature at regional scales over the past 50 years and illustrate the trustworthiness of their predictions. Most of the skill can be attributed to changes in atmospheric composition, but also partly to the initialization of the predictions.
The establishment of Local Action Groups (LAGs) within the framework of LEADER with the participation of public and private actors through a bottom–up approach (i.e., the empowerment of local ...society) and the management of local development strategies constitutes one of the major innovations in the field of rural policy in Spain. The protagonism of local society and the local management of development processes entail the introduction and experimentation of previously unknown mechanisms of territorial governance. However, the efficacy of this rhetoric has been seriously limited in its practical implementation, with difficulties conceiving truly integrated and multi-sectoral strategies, increasing bureaucratization and the progressive exhaustion of local actors. Yet the factor most responsible for slowing the progress of LAGs and LEADER has been the conception and use of them as clientelistic and power instruments by local and regional elites (mainly composed of public actors).
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•LAGs play an important role in fostering economic development in rural areas in Spain.•LAGs have been able to foster many individual actions, but rarely comprehensive strategies.•LAGs are increasingly encouraging capacity-building, democracy, local empowerment and governance, and gaining legitimacy and leadership.•From LEADER Plus onwards, the exhaustion of stakeholders and an increased workload have hindered effectiveness.•From LEADER Plus onwards, many LAGs have become mere instruments of power and clientelism, thereby losing legitimacy.
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