The research elucidates the stress degradation behavior of Ertugliflozin, which is used for the treatment of type-2 diabetics. The degradation was conducted as per ICH guidelines and Ertugliflozin is ...relatively stable in thermal, photolytic, neutral, and alkaline hydrolysis conditions; however, considerable degradation was detected in acid hydrolysis and oxidative hydrolysis. Degradation products were identified by ultra-high-performance liquid chromatography-mass spectrometry, isolated by semi-preparative high-performance liquid chromatography, and structural characterization using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Total four degradation products were identified and isolated in acid degradation, which are degradation products 1, 2, 3, and 4. Whereas in oxidative conditions, degradation product 5 was identified. All the five degradation products formed are novel, which was not reported earlier. This is the first time documented complete structural characterization of all five degradation products by using a hyphenated analytical technique. High-resolution mass, and nuclear magnetic resonance spectroscopy were used in the present study to get concrete confirmation of degradation products structures. The current method is also used to identify degradation products with shorter runtime in the future.
The current research explains the stress degradation behavior of Apixaban, which is an anticoagulant or blood thinner. The degradation was conducted using hydrolytic, oxidative, thermal, and ...photolytic conditions. Apixaban is relatively stable in oxidative, thermal, and photolytic conditions; however, considerable degradation was observed in acid and base hydrolysis. Degradation products were identified using ultra‐high performance liquid chromatography‐mass spectrometry, isolated using semi‐preparative high‐performance liquid chromatography, and structural characterization by high‐resolution mass spectrometry and nuclear magnetic resonance spectroscopy. A total of five degradation products were identified and isolated in acid and base degradation. Degradation products 1, 2, and 3 were observed in acid conditions, whereas in base conditions, along with those three, two more degradation products 4 and 5 were identified. The representative thing was that among the five degradation products, two sets of positional isomers 1, 4, and 2, 5 were observed; out of which 2 and 5 are novel. The remaining degradation products 1, 3, and 4 are already reported tentatively using a single analytical technique of mass analysis without any evidence from nuclear magnetic resonance spectroscopy. Hence, the present study focused on using high‐resolution mass, and nuclear magnetic resonance spectroscopy data for concrete confirmation of structures for degradation products.
In an effort to develop potent antibacterial and anticancer agents, a series of C5′-N-cyclopropylcarboxamido-C6-amino-C2-alkynylated purine nucleoside analogues 11a-g were synthesized through a ...Sonogashira cross-coupling reaction. The nine-step synthesis is easy to perform, and employs commercially available reagents. 2-Iodo-5′-N-cyclopropylcarboxamidoadenosine (9) was used as the starting intermediate for the synthesis of title derivatives 11a-g. Synthetic intermediates (2-9) and final products (11a-g) were appropriately characterized by IR,
1
H NMR,
13
C NMR and mass spectroscopy. The synthesized purine nucleoside analogues (11a-g) were evaluated for their in vitro antibacterial activity against two gram-positive and two gram-negative bacteria. They were then tested for cytotoxicity against MDA-MB-231 and Caco-2 cancer cell lines to determine their anti-cancer activity. Among the tested compounds, compounds 11c and 11g showed most potent antibacterial activity against S.aureus and P.aeruginosa bacterial strains. Compounds 11b and 11e displayed considerable IC
50
s
of 7.9 and 6.8 µg/mL, respectively, vs MDA-MB-231 cell lines of 7.5 and 8.3 µg/mL, respectively, against the Caco-2 cell lines.
This research uses computational analytical tools to study the impact of nano-dopants on the characteristics of liquid crystals. The main focus of the study is on the conformation analysis and degree ...of interaction between liquid crystal materials and nano-dopants. FTIR, UV visible spectroscopy, and thermal microscopic techniques are among the spectroscopic methods employed to analyze the information. Furthermore, computationally driven approaches are used to gain a deeper understanding of how nano-dopants influence the characteristics of materials. The molecular interactions between the various functional groups of pure and nanocomposite materials can be investigated by utilizing the wavelet transform on FTIR spectrum data. Additionally, the presence and influence of nano-dopants on liquid crystals are investigated through polarized optical microscopy (POM) and correlation studies of optical texture analysis with UV visible spectroscopy. Here, p, n-alkoxybenzoic acid (nOBA, where n = 7,9) doped with magnetic nanoparticles (Fe3O4) of size 20nm is considered for investigation. The findings from this study show the successful utilization of computational methods to analyze the effect of nano-dopants on the characteristics of liquid crystals and their potential use in the development of biosensor devices.
A new series of chalcones
were synthesized and evaluated for
antiproliferative activity against human colon cancer cell lines. The synthesis of the key intermediate compounds
was achieved by ...tetrakis(triphenylphosphine) palladium(II) mediated Suzuki cross coupling reaction. Chalcone
shows superior anticancer activity with IC
value of 21.0 μg/mL compared to the IC
value of the reference drug doxorubicin at 21.65 μg/mL.
Montmorillonite K10 is a suitable catalyst in a multicomponent reaction involving an aldehyde, an amine, and thioglycolic acid in
-dimethylformamide as solvent at moderate (50°C) to reasonably high ...(120°C) temperatures to form thiazolidinones. The reaction involves easy workup and purification. Several thiazolidinones were prepared. In particular, campholenic aldehyde obtained from α-pinene was used to synthesize potentially bioactive thiazolidinones. All products were characterized by IR,
H NMR, and mass spectra. Preliminary anticancer screening tests revealed that two compounds show anticancer activity and can be taken up for further screening.
This study utilizes spectral analysis techniques and various computational methods to examine the impact of hydrogen bonding on the optical characteristics of supramolecular liquid crystals within ...the UV-visible spectrum.This investigation involves the computational method of spectral denoising for quantitative analysis of UV-visible spectra, and then spectral analysis is carried out for the measurement of the optical properties of supramolecular liquid crystals. Other computational methods, like the texture absorbance method, are also employed for colorimetric studies of compounds.The investigation will focus on supramolecular systems:p,n- alkoxy benzoic acid: Do-decyl hydroxybenzoate (nOBA:12HB), where n = 7, 8, and 9. The supramolecular system is formed via hydrogen bond development between the OH acceptor of the non-liquid crystalline substance Do-decyl hydroxyl benzoate (12HB) and the COOH donor of the liquid crystal p-n alkoxy benzoic acid. Here, optical properties: such as absorption coefficient, optical energy band gap, Urbach energy, and refractive index of supramolecular liquid crystals are measured for the denoised spectral data. The influence of hydrogen bonding and alkoxy chain length on the optical properties of liquid crystals has been deliberated upon. Further, the attempt at computation studies on the spectral data will yield important insights for subsequent investigations into UV-visible spectral data measurements.
In this paper, the self-assembling nature of nano-doped liquid crystals is characterized using different techniques. Liquid crystals, namely 4, n-alkoxybenzoic acid (nOBA, where n = 7,8), doped with ...magnetic nanoparticles (Fe3O4 of size 20nm) are considered for the present study. The structural and morphological properties of nano-doped liquid crystals are determined by the techniques of X-ray diffraction and Scanning Electron Microscopy. Morphological properties like grains, grain boundaries, and crystal defects are elucidated using the scanning electron microscope technique. Structural properties such as crystal structure, grain size, inter-atomic spacing, lattice constant, and dislocation density are determined using X-ray diffraction studies. The characteristics measured from the X-ray diffraction studies are correlated using the statistical analysis of Scanning electron microscope images. This is an objective analysis of X-ray diffraction studies. In addition to these techniques, colorimetric studies of optical anisotropic liquid crystals are carried out by measuring the chromaticity coordinates and color purity. This study utilizes the polarizing optical microscope technique. Furthermore, the influence of nano dopants on the characteristics of liquid crystals with carbon chain length is also discussed.
Synthesis, characterization and biological activity of novel 4,5-diaryl/heteroaryl thiophene-2-carboxylic acid derivatives are described. Aryl/heteroaryl esters were converted to substituted ...thiophene esters via a Vilsmeier-Haack reaction, which were then hydrolyzed to 4,5-diaryl/heteroaryl thiophene-2-carboxylic acid derivatives
. All products were characterized by
H NMR,
C NMR, IR and MS. Antibacterial activity of the synthesized compounds against two Gram-positive and two Gram-negative bacteria, as well as their anticancer activity against PC-3 cell line (human prostate cancer cell lines) were evaluated.
