Global modeling of nature's contributions to people Chaplin-Kramer, Rebecca; Sharp, Richard P; Weil, Charlotte ...
Science (American Association for the Advancement of Science),
10/2019, Letnik:
366, Številka:
6462
Journal Article
Recenzirano
The magnitude and pace of global change demand rapid assessment of nature and its contributions to people. We present a fine-scale global modeling of current status and future scenarios for several ...contributions: water quality regulation, coastal risk reduction, and crop pollination. We find that where people's needs for nature are now greatest, nature's ability to meet those needs is declining. Up to 5 billion people face higher water pollution and insufficient pollination for nutrition under future scenarios of land use and climate change, particularly in Africa and South Asia. Hundreds of millions of people face heightened coastal risk across Africa, Eurasia, and the Americas. Continued loss of nature poses severe threats, yet these can be reduced 3- to 10-fold under a sustainable development scenario.
•Deep surface rolling as a post-repair enhancement technology was applied to the laser cladded 7075-T651 aluminium alloy specimens that simulated corrosion damage blend-out repair.•The residual ...stresses induced by the deep surface rolling process were measured.•The deep surface rolling process can introduce deep and high magnitude compressive residual stresses beyond the laser clad and substrate interface.•Spectrum fatigue test showed the fatigue life was significantly increased by deep surface rolling.
Deep surface rolling can introduce deep compressive residual stresses into the surface of aircraft metallic structure to extend its fatigue life. To develop cost-effective aircraft structural repair technologies such as laser cladding, deep surface rolling was considered as an advanced post-repair surface enhancement technology. In this study, aluminium alloy 7075-T651 specimens with a blend-out region were first repaired using laser cladding technology. The surface of the laser cladding region was then treated by deep surface rolling. Fatigue testing was subsequently conducted for the laser clad, deep surface rolled and post-heat treated laser clad specimens. It was found that deep surface rolling can significantly improve the fatigue life in comparison with the laser clad baseline repair. In addition, three dimensional residual stresses were measured using neutron diffraction techniques. The results demonstrate that beneficial compressive residual stresses induced by deep surface rolling can reach considerable depths (more than 1.0mm) below the laser clad surface.
Abstract
Inhibitory interneurons can evoke vasodilation and vasoconstriction, making them potential cellular drivers of neurovascular coupling. However, the specific regulatory roles played by ...particular interneuron subpopulations remain unclear. Our purpose was therefore to adopt a cell-specific optogenetic approach to investigate how somatostatin (SST) and neuronal nitric oxide synthase (nNOS)-expressing interneurons might influence the neurovascular relationship. In mice, specific activation of SST- or nNOS-interneurons was sufficient to evoke hemodynamic changes. In the case of nNOS-interneurons, robust hemodynamic changes occurred with minimal changes in neural activity, suggesting that the ability of blood oxygen level dependent functional magnetic resonance imaging (BOLD fMRI) to reliably reflect changes in neuronal activity may be dependent on type of neuron recruited. Conversely, activation of SST-interneurons produced robust changes in evoked neural activity with shallow cortical excitation and pronounced deep layer cortical inhibition. Prolonged activation of SST-interneurons often resulted in an increase in blood volume in the centrally activated area with an accompanying decrease in blood volume in the surrounding brain regions, analogous to the negative BOLD signal. These results demonstrate the role of specific populations of cortical interneurons in the active control of neurovascular function.
RNA interference--2001 Sharp, P A
Genes & development,
2001-Mar-01, 2001-03-01, 20010301, Letnik:
15, Številka:
5
Journal Article
Recenzirano
Odprti dostop
In the few years since the discovery of RNA interference (RNAi), it has become clear that this process is ancient. RNAi, the oldest and most ubiquitous antiviral system, appeared before the ...divergence of plants and animals. Because aspects of RNAi, known as cosuppression, also control the expression of transposable elements and repetitive sequences, the interplay of RNAi and transposon activities have almost certainly shaped the structure of the genome of most organisms. Surprisingly, we are only now beginning to explore the molecular processes responsible for RNAi and to appreciate the breadth of its function in biology. Practical applications of this knowledge have allowed rapid surveys of gene functions and will possibly result in new therapeutic interventions. Genetic studies have expanded the biology of RNAi to cosuppression, transposon silencing, and the first hints of relationships to regulation of translation and development. The possible roles of RNA-dependent RNA polymerase (RdRp) in RNAi have been expanded. Many experiments indicate that dsRNA directs gene-specific methylation of DNA and, thus, regulation at the stage of transcription in plants. Cosuppression may involve regulation by polycomb complexes at the level of transcription in C. elegans and Drosophila. This article will review these topics and primarily summarize advances in the study of RNAi over the past year.
siRNAs can function as miRNAs Doench, John G; Petersen, Christian P; Sharp, Phillip A
Genes & development,
02/2003, Letnik:
17, Številka:
4
Journal Article
Recenzirano
Odprti dostop
With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a short interfering RNA ...(siRNA) can repress expression of a target mRNA with partially complementary binding sites in its 3' UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs). The mechanism for this repression is cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs.
The agricultural expansion and intensification required to meet growing food and agri-based product demand present important challenges to future levels and management of biodiversity and ecosystem ...services. Influential actors such as corporations, governments, and multilateral organizations have made commitments to meeting future agricultural demand sustainably and preserving critical ecosystems. Current approaches to predicting the impacts of agricultural expansion involve calculation of total land conversion and assessment of the impacts on biodiversity or ecosystem services on a per-area basis, generally assuming a linear relationship between impact and land area. However, the impacts of continuing land development are often not linear and can vary considerably with spatial configuration. We demonstrate what could be gained by spatially explicit analysis of agricultural expansion at a large scale compared with the simple measure of total area converted, with a focus on the impacts on biodiversity and carbon storage. Using simple modeling approaches for two regions of Brazil, we find that for the same amount of land conversion, the declines in biodiversity and carbon storage can vary two- to fourfold depending on the spatial pattern of conversion. Impacts increase most rapidly in the earliest stages of agricultural expansion and are more pronounced in scenarios where conversion occurs in forest interiors compared with expansion into forests from their edges. This study reveals the importance of spatially explicit information in the assessment of land-use change impacts and for future land management and conservation.
Mutations affect individual health, population persistence, adaptation, diversification, and genome evolution. There is evidence that the mutation rate varies among genotypes, but the causes of this ...variation are poorly understood. Here, we link differences in genetic quality with variation in spontaneous mutation in a Drosophila mutation accumulation experiment. We find that chromosomes maintained in low-quality genetic backgrounds experience a higher rate of indel mutation and a lower rate of gene conversion in a manner consistent with condition-based differences in the mechanisms used to repair DNA double strand breaks. These aspects of the mutational spectrum were also associated with body mass, suggesting that the effect of genetic quality on DNA repair was mediated by overall condition, and providing a mechanistic explanation for the differences in mutational fitness decline among these genotypes. The rate and spectrum of substitutions was unaffected by genetic quality, but we find variation in the probability of substitutions and indels with respect to several aspects of local sequence context, particularly GC content, with implications for models of molecular evolution and genome scans for signs of selection. Our finding that the chances of mutation depend on genetic context and overall condition has important implications for how sequences evolve, the risk of extinction, and human health.
Among the 3 billion base pairs of the human genome, there are approximately 30,000-40,000 protein-coding genes, but the function of at least half of them remains unknown. A new tool - short ...interfering RNAs (siRNAs) - has now been developed for systematically deciphering the functions and interactions of these thousands of genes. siRNAs are an intermediate of RNA interference, the process by which double-stranded RNA silences homologous genes. Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology.
The deleterious mutation rate plays a key role in a number of important topics in biology, from mating system evolution to human health. Despite this broad significance, the nature and causes of ...variation in mutation rate are poorly understood, especially in multicellular organisms. We test whether genetic quality, the presence or absence of deleterious alleles, affects the mutation rate in Drosophila melanogaster by using a modified mutation accumulation approach. We find evidence that genotypes constructed to carry deleterious "treatment" alleles on one chromosome during mutation accumulation experience an elevated mutation rate on a different chromosome. Further, this elevation is correlated with the effect of the treatment alleles on phenotypic condition, measured as body mass. Treatment alleles that reduce mass by 10% cause a doubling in the rate of mutational decline. Our results show that mutation rates are sensitive to genetic stress, such that individuals with low-quality genotypes will produce offspring of even lower genetic quality, in a mutational positive feedback loop. This type of variation in mutation rate is expected to alter a variety of predictions based on mutation load theory and accelerate adaptation to new environments. Positive mutational feedback could affect human health by increasing the rate of germline mutation, and possibly somatic mutation, in individuals of poor health because of genetic or environmental stress.
The integrator SSS performs accurate
N
-body simulations of the Solar System when there is a mix of massive bodies and test particles. The orbital motion of all bodies at all times is integrated ...using a 12-10 explicit Runge-Kutta Nyström (RKN) pair. The test particles are divided into sets and each set integrated on a different processor. The explicit RKN pair uses an order 12 interpolant for the position and velocity when checking for collisions. We report on two significant improvements to SSS. The first improvement reduced the local round-off error in interpolated values by approximately four orders of magnitude, permitting more accurate modelling of collisions. The technique used to reduce the round-off error can be applied to other high-order interpolants. The second improvement is hand optimization of the implementation of SSS. This optimization increased the speed of SSS by approximately 60%, permitting more accurate modelling through the use of more test particles. We also present a summary of the numerical performance of SSS on a simulation of the Sun, the planets Earth to Neptune, and 500,000 test particles over 100 million years.
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