A
bstract
The production of
J/ψ
mesons is studied with the LHCb detector using data from
pp
collisions at
TeV corresponding to an integrated luminosity of 71 nb
−1
. The differential cross-section ...for inclusive
J/ψ
production is measured as a function of its transverse momentum
p
T
. The cross-section in the fiducial region 0
< p
T
<
12 GeV
/c
and rapidity 2.0
<
<
4.5 is measured to be 5.6 ± 0.1 (stat) ± 0.4 (syst)
μ
b, with the assumption of unpolarised
J/ψ
production. The fraction of
J/ψ
production from
b
-hadron decays is measured to be (7
.
1 ± 0
.
6 (stat) ± 0
.
7 (syst))%.
The effect of low intensity microwaves on the conformational state of the genome of X-irradiated E. coli cells was studied by the method of viscosity anomalous time dependencies. It has been ...established that within the ranges of 51.62-51.84 GHz and 41.25-41.50 GHz the frequency dependence of the observed effect has a resonance nature with a resonance halfwidth of the order of 100 MHz. The power dependence of the microwave effect within the range of 0.1-200 (μW/cm
has shown that a power density of 1 μW/cm
is sufficient to suppress radiation-induced repair of the genome conformational state. The effect of microwave suppression of repair is well reproduced and does not depend on the sequence of cell exposure to X-rays and microwave radiation in the millimeter band. The results obtained indicate the role of the cell genome in the resonant interaction of cells with low intensity millimeter waves.
The pLF1311 natural plasmid from Lactobacillus fermentum 1311 was used to construct a single-replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and Escherichia coli. ...The new vector is capable of conjugative mobilization from E. coli to various hosts by conjugal transfer. The final vector (3.4 kb) showed a high segregational and structural stability and a high copy number. Glutamyl endopeptidase genes from Bacillus licheniformis (gseBL) and B. intermedius (gseBI) were cloned in both pLF9 and pLF14 vectors and introduced to B. subtilis. The yield of enzymes in the pLF-derived producers was 6- to 30-fold more than in the natural producers and reached 100–150 mg/L of mature protease.
A modified method for preparing barium and lead titanate films is suggested, which combines precipitation from solution and elements of sol-gel processing. The ∼1-µm-thick BaTiO3/Pt and PbTiO3/Pt ...films deposited on Pt foil are transparent, show good adhesion, and have the 1 : 1 : 3 stoichiometry. The films are finegrained, with a mean grain size of 0.1–0.2 µm. The barium titanate films are characterized bytC = 110-125°C, ε20 = 167-500, tanδ20 = 0.015-0.06, εmax = 1050,Ps= 3.2 µC/cm2,Ec = 14 kV/cm, ρ = (5-7) × 109 Ω cm, andVbr = 80-150 kV/cm. The lead titanate films are characterized byc/a = 1.056, ε20 = 105-110, tanδ20 = 0.11-0.16,Ps = 4.1 µC/cm2,Ec = 34 kV/cm, ρ = (0.8-3.0) × 109 Ω cm, andVbr = 50-100 kV/cm.
Single-crystal Pb5Ge3O11 films 5–105 μm thick, adhering well to platinum substrates, were prepared by pyrolysis of metalorganic precursors in combination with in situ sol-gel processing. The best ...films consisted of densely packed crystallites ranging in size from 200 to 300 μm, with hexagonal habits. A welldefined ferroelectric transition was revealed at 170–180°C. The typical parameters of the films are ɛ20 = 30–40, tan δ ≃0.02, ɛmax ≃200, Ps = 3.2 μC/cm2,Ec = 16 kV/cm, and ρ = 108-109 Ω cm, in agreement (except for ɛmax) with those of lead germanate crystals
The significant difference in the effects of right- and left-handed polarized microwaves of millimeter range (MMW) on Escherichia coli cells and rat thymocytes has been shown previously. The helicity ...of DNA and supercoiling of DNA loops was suggested to cause the observed dependence of effects on polarization. It is known that DNA intercalators such as ethidium bromide (EtBr) are able to change the supercoiling of DNA loops and to affect the DNA helicity. In the present work, the combined effects of EtBr (1 μg/ml) and circularly polarized microwaves were studied in E. coli cells K12 AB1157 at the stationary stage of growth. The method of anomalous viscosity time dependencies (AVTD) was used to measure changes in the conformation of the genome. The cells were exposed to microwaves at 51.755 GHz and 0.1 mW/cm
2
for 10 min. Under these conditions of exposure, left-handed microwaves induced changes in the genome conformation, whereas right polarization was almost ineffective. The incubation of cells with EtBr inverted the effective polarization, and right-handed MMW became more effective than left polarization. The data obtained provide new evidence that DNA is a target of MMW effects on cells.
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