Immune checkpoint proteins (ICPs) play a major role in a patient's immune response against cancer. Tumour cells usually express those proteins to communicate with immune cells as a process of ...escaping the anti-cancer immune response. Detecting the major functional immune checkpoint proteins present on cancer cells (such as circulating tumor cells or CTCs) and examining the heterogeneity in their expression at the single-cell level could play a crucial role in both cancer diagnosis and the monitoring of therapy. In this study, we develop a mesoporous gold biosensor to precisely assess ICP heterogeneity in individual cancer cells within a lung cancer model. The platform utilizes a nanostructured mesoporous gold surface to capture CTCs and a Surface Enhanced Raman Scattering (SERS) readout to identify and monitor the expression of key ICP proteins (PD-L1, B7H4, CD276, CD80) in lung cancer cells. The homogeneous and abundant pores in mesoporous 3D gold nanostructures enable increased antibody loading on-chip and an enhanced SERS signal, which are key to our single cell capture, and accurate analysis of ICPs in cancer cells with high sensitivity. Our lung cancer cell line model data showed that our method can detect single cells and analyse the expression of four lung cancer associated ICPs on individual cell surfaces during treatment. To show the potential of our mesoporous gold biosensor in analysing clinical samples, we tested 9 longitudinal Peripheral Blood Mononuclear Cells (PBMC) samples from lung cancer patient before and after therapy. Our mesoporous biosensor successfully captured single CTCs and found that the expression of ICPs in CTCs is highly heterogeneous in both pre-treatment and treated PBMC samples isolated from lung cancer patient blood. We suggest that our findings will help clinicians in selecting the most appropriate therapy for patients.
I.I.Tosheva, G.A.Ikhtiyarova Abu Ali ibn Sina Bukhara State Medical Institute, Bukhara, Uzbekistan Aim: to analyze causative factors, obstetrical, and perinatal outcomes in women with preterm ...premature rupture of the membranes (PPROM) and to develop delivery strategy. Patients and Methods: medical records of 106 deliveries (in 2017–2019) in women with PPROM occurred at 37–39 weeks of gestation were analyzed. Anamnestic somatic and obstetrical gynecological data were assessed. The course of current and previous pregnancies, delivery, and postnatal period was described in detail. Laboratory tests, vaginal flora, and Bishop Score used to rate the readiness of the birth canal for labor and other factors (i.e., bleeding, birth defects, antenatal death, chorioamnionitis signs etc.) were evaluated as well. In addition, uterine and fetal ultrasound was performed. Results: mean age of study women was 26.5 years. All women had obstetrical, gynecological, or somatic comorbidities. 22 women with PPROM (20.7%) were characterized by low socieconomic status. 11 women (11.3%) had bad habits, i.e., drug and alcohol addiction, 20.7% (22 women) occupational hazards, and 30.2% (32 women) compromised family history. Conclusions: retrospective analysis of delivery records has demonstrated that compromised obstetrical, gynecological, and somatic history (which occurred in all women) was the key factor promoting PPROM.PPROM resulting from the pathological growth of cervical vaginal flora causes chorioamnionitis in 26.4% of cases thus accounting for significant increase in the prevalence of obstetrical disorders. Keywords: pregnancy, chorioamnionitis, amnion, preterm premature rupture of the membranes, labor induction, vaginal microflora. For citation: Tosheva I.I., Ikhtiyarova G.A. Pregnancy outcomes in preterm premature rupture of the membranes. Russian Journal of Woman and Child Health. 2020;3(1):–19. DOI: 10.32364/2618-8430-2020-3-1-16-19.
A new family of luminescent platinum(II) acetylide complexes and polymers were formed by the copper(I) catalyzed reaction of
cis
-PtCl
2
(PR
3
)
2
(R=C
6
H
5
–
p
-CH
3
) with appropriate acetylide ...ligands. The reaction of metal precursors with 2.5 equivalents of monoterminal acetylide ligands provided metal complexes
trans
-Pt(
p
-tolyl
3
P)
2
(C≡C-R)
2
(R=C
6
H
4
-
p
-NO
2
(
1
) C
6
H
4
-
p
-CH
3
(
2
)), and equimolar amounts of diterminal ligand and metal chloride precursor, under reflux, afforded the metal poly-yne polymers -Pt(
p
-tolyl
3
P)
2
C≡C–R–C≡C–
n
, (R=biphenyl and 2,5-dioctyloxybenzene). Characterization of the newly developed polymer and metal complexes was accomplished by FT-IR, multinuclear NMR (
1
H,
31
P,
13
C) and mass spectrometry, as well as elemental analysis. The molecular structure of the metal complex
trans
-Pt(
p
-tolyl
3
P)
2
(C≡CC
6
H
4
–
p
-NO
2
)
2
(
1
) was confirmed by single crystal X-ray crystallography. The electronic absorption and photoluminescence spectra of the metal complexes and polymers have been used to probe their photophysical properties. The studies reveal that the presence of heavy metal atom and substituent groups on the phenyl ring of the ligands can enhance the efficiency of intersystem crossing from the S
1
singlet excited state to the T
1
triplet excited state and thus give intense phosphorescence.
Endoscopy and tissue biopsy are the gold standard methodology of diagnosing esophageal adenocarcinoma (OAC) and its premalignant condition, Barrett’s esophagus (BO). This procedure is invasive and ...unsuitable for population screening, leading to late diagnosis and poor prognosis of most OAC. In this context, identifying the likelihood of OAC and/or BO with a pre-endoscopic population-based screening test utilizing liquid biopsies would be an ideal method to stratify patients for endoscopy. Herein, we develop a surface-enhanced Raman scattering lectin-immunoassay (SERS-LIA) to accurately measure serum jacalin (JAC) lectin-binding complement C9 (JAC-C9), JAC-gelsolin (JAC-GSN), and JAC-prekallikrein (JAC-KLKB1). Multiplexing via plasmonic nanoparticles enables sensitive serum detection of 30 ng mL–1 for JAC-C9, 53 ng mL–1 for JAC-GSN, and 23 ng mL–1 for JAC-KLKB1. The diagnostic performance of SERS-LIA was evaluated on 42 serum samples (15 healthy controls, 16 BO, and 11 OAC). Multilinear regression analysis (MLR) achieved excellent receiver operating characteristic (ROC) curves with an area under the curve of 1.00 for OAC versus BO, 1.00 for OAC versus healthy, and 0.98 for OAC versus BO. These findings suggest that our developed multiplex SERS-LIA could be a promising liquid biopsy platform for OAC screening.
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•A SERS DNA biosensor is developed for simultaneous detection of two different species.•A unique design in Raman dye integrated DNA probe contributes in specific and sensitive ...detection of trace amount of DNA.•Graphene and gold nanoparticle justifies as suitable platform in SERS signal enhancement.•Biosensor is validated for simultaneous and quantitative analysis of real samples.
Development of a facile, fast, sensitive and multiplex DNA detection for the quantitative verification of food adulterant is of high significance these days. RT-PCR, and nanoparticle based fluorescence and electrochemical detection techniques have been successfully used in clinical medicine. However, requirements of pre-conditioning steps and fluorescence dye labeling in RT-PCR, inherent photobleaching and overlapping spectra of fluorescent probe in fluorescent assay, are the few drawbacks that urge to find a suitable alternative. Surface-enhanced Raman scattering (SERS) provides molecule specific fingerprint spectra, could be a strategic to resolve the limitations. Here, we report a SERS based duplex DNA detection strategy for simultaneous and quantitative detection of a meat adulterant (pork) and an endangered species – Malayan Box Turtle (MBT). In the biosensing strategy, SERS active dual platforms – graphene oxide-gold nanoparticles (GO-AuNPs) and AuNPs, and uniquely designed Raman tag intercalated short-length signal probe (SP) sequences are used. The sensing principle relies on the covalent linking of SP sequences functionalized AuNPs and capture probe (CP) functionalized GO-AuNPs via hybridization with corresponding target DNA. Coupling of multi-component platforms contributes in huge SERS signal enhancement due to electromagnetic and charge transfer mechanism. The biosensor showed an improved sensitivity in simultaneous detection of both adulterants with limit of detection (LOD) is 1 × 10−14 M. Moreover, efficiency of SERS biosensor was validated by the DNA extracted from real samples with a LOD of 1 × 10-13 M. Furthermore, the biosensing approach showed excellent sequence specificity in discriminating DNA sequences of five non-target species and specificity towards single nucleotide differentiation.
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