The main purpose of this study was to determine pesticide contamination levels in commonly consumed vegetable and fruit samples collected from three different regions of Gujarat state, India. Samples ...(n = 312) were collected and extracted using modified QuEChERS method and quantified for presence of residues of 52 pesticides (organochlorine, organophosphate, carbamate and synthetic pyrethroids) by GC-MS/MS and UHPLC-QTOF/MS. Of the 52 pesticides, residues of 12 pesticides were detected and bifenthrin, fenvalerate and cypermethrin were frequently detected in maximum number of samples. The results observed that, 98.8 % tomato, 97.5 % banana, 90 % eggplant, 88.8 % pomegranate, 83.8 % orange, 75 % okra and 66.3 % of green chilli samples were below the Maximum Residue Limit (MRL). Acute and chronic dietary risk assessment was calculated due to consumption of vegetable and fruit samples for the Indian adolescent and adult population and results showed that the majority of samples had HQ (Hazard Quotient) and HI (Hazard Index) values were less than 1. Overall results revealed that, the studied commodities were safe for human consumption. However, monitoring studies may be carried out to protect the consumers.
•Pesticide determination and health risk assessment in vegetables and fruits.•Modified QuEChERS extraction method followed by d-SPE clean-up employed.•52 Multi-class pesticides analyzed using GC-MS/MS and UHPLC-QTOF/MS.•aHI and cHI above 1 for certain commodities indicating a possible hazard to human health.•Long-term monitoring and awareness programs must be implemented.
Display omitted
•Melatonin albumin nanoparticle (MNP) embedded polycaprolactone scaffold fabricated.•Controlled release observed for 22 days.•MNP concentration modulated melatonin release from ...scaffolds.•Significant elevation in Glycosaminoglycans - Human chondrocytes.•Diffusion & dissolution mechanism of drug release – mathematical models.
The therapeutic potential of an engineered cartilage construct can be enhanced by sustained delivery of chondrogenic drug like melatonin from 3D porous scaffolds embedded with melatonin loaded bovine serum albumin nanoparticles (MNP). In this study, MNP was synthesized and loaded into polycaprolactone (PCL) scaffolds. 12 % (w/v) and 10 % (w/v) PCL scaffolds were fabricated with different concentrations of MNP. X- ray diffraction and Raman analysis of MNP and scaffolds revealed amorphization of melatonin which is highly desired in drug delivery applications. Additionally, Fourier Transform Infrared spectroscopic analysis confirmed the drug to be chemically inert to fabrication process. Field emission scanning electron microscopic analysis suggested highly interlinked porous scaffold (diameter 50 μm – 300 μm) and MNP diameters in the range of 110−200 nm. Importantly, UV spectrophotometric analysis showed that all groups of scaffolds showed sustained release for 21 days, wherein MNP concentration had an influence on release behaviour of melatonin from scaffolds. Drug release kinetics studied using mathematical models revealed, diffusion and dissolution mechanism of release. Furthermore, in vitro evaluation of MNP loaded scaffolds with Human chondrocytes for 21 days increased glycosaminoglycans deposition significantly. In brief, sustained release of melatonin from polycaprolactone scaffolds increased the therapeutic potential of the engineered construct.
•A new multiresidue method for the quantitation of pesticide residues by UHPLC–TOF/MS.•The method is fast (5min) with high mass accuracy.•Simultaneous determination of 60 pesticide residues in ...vegetables and fruits.•This analytical method is environmentally friendly.
A multiresidue method, based on the sample preparation by solid-phase extraction cartridges and detection by ultra-high-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOF–MS), was used for the analysis of 60 pesticides in vegetable and fruit samples. Quantitation by UHPLC/TOF–MS is accomplished by measuring the accurate mass of the protonated molecules M+H+. The mass accuracy typically obtained is routinely better than 2ppm. The rates of recovery for pesticides studied were satisfactory, ranging from 74% to 111% with a relative standard deviation (RSD) of less than 13.2%, at concentrations below 10μgkg−1. The method limit of quantification (MLOQ) for most compounds was below the MRLs established by the Food Safety Standard Authority of India and the European Union. The uncertainty was determined using repeatability, recovery and calibration curves data for each pesticide. The method illustrated is suitable for routine quantitative analyses of pesticides in food samples.
On 4th December 2020, a sudden outbreak, with neurological symptoms like seizures, loss of consciousness etc., was reported in a town from south India. By 3rd day about 400 people were involved. A ...multi disciplinary team from our institute visited the site to investigate the outbreak. Based on the case history and clinical examination of the patients, the team suspected a probable diagnosis of an acute pesticide, heavy metal and/or mycotoxin exposure for which, biological samples (blood, urine) were collected from those who reported the symptoms as well as from a few who did not report symptoms (controls). To identify the source, water and food samples were collected. The samples were subjected to ICP-MS for heavy metal analysis, LC-MS/MS for pesticide analysis, microbiological analysis and ELISA-Kit method for aflatoxins if any. Clinical and dietary details were collected from a total of 112 participants, of which, 103 cases (77 active cases at Hospital and 26 recovered cases from community) and 9 were controls. A total of 109 biological samples, 36 water samples and food samples were collected. The mean age of the study participants was 29.2 years. Among cases, Seizures were seen in 84%, loss of consciousness in 66%, mental confusion in 35%, pinpoint pupil in 11%. Triazophos (organophosphate) pesticide was present in 74% of Blood samples and its metabolites were present in 98% of the urine samples collected from the cases. All the ten heavy metals investigated including lead, mercury and nickel were found to be within permissible limits except for a few samples. No presence of mycotoxins was observed in Food samples. Water samples which included Head pump and reservoir were free from pesticides; however, all water samples from households of cases had triazophos pesticide with a mean concentration of 1.00 ug/L. Thus, it was concluded that, the probable cause of outbreak was Triazophos (Organophosphate) pesticide contamination in water at the Household level. Regular surveillance for the presence of residual pesticides in soil, water and food with heightened vigour is recommended to prevent future outbreaks.
Three species of mangroves and six species of salt marshes were collected from various locations along the Tuticorin coast for the estimation of metals like Cu, Cd, Pb, and Zn. The bioaccumulation of ...metals in mangroves is in the order of Cu > Pb > Zn > Cd, and the season-wise accumulation was higher in monsoon followed by summer, post-monsoon, and pre-monsoon seasons. The accumulation of metals in mangroves showed higher in Rhizophoraapiculata followed by Avicenniamarina, and Rhizophoramucronata. The concentration of metals in salt marshes is in the order of Pb > Zn > Cu > Cd, and the species-wise accumulation showed in the order of Suaeda maritime > Ipomoea sp. > Suaeda sp. > Spinifix littoreous > Sesuvium portacastrum > Ipomoea pes-capras. BAF in salt marhes and mangroves is as Cd < Pb < Cu < Zn and Cd < Cu < Pb < Zn, respectively.
Display omitted
•Three species of mangroves and six species of saltmarshes were collected from Tuticorin for the estimation of metals.•Among the metals tested, the Pb and Cd contents are more and over all accumulation showed more during monsoon season.•The metals contents in mangrove and saltmarshes are in the order of Cu > Pb > Zn > Cd and Pb > Zn > Cu > Cd respectively.•Bioaccumulation Factor in saltmarhs and mangroves are as Cd < Pb < Cu < Zn and Cd < Cu < Pb < Zn respectively.•The present study recommends regular monitoring and strict enforcement of law for discharge of effluents from industries.
Objective
The systemic illnesses associated with chronic lead exposure are partially explained by the interaction between lead and calcium metabolism. Lead exposure is posited to alter calcium levels ...either by altering calcium homeostasis markers or altering bone remodeling. The present study investigated the interaction between blood lead levels and calcium homeostasis markers and bone remodeling markers among lead-smelting plant workers.
Method
Adult male workers employed at the lead-smelting plant were clinically investigated as part of their regular occupational health assessment program. Additionally, control participants without occupational lead exposure, employed in administrative and white-collar jobs were invited to participate in the study. Sociodemographic and occupational details were collected by pre-standardized semi-structured questionnaires from all consenting participants, followed by clinical examination and blood collection. Blood lead levels were estimated using microwave-assisted acid digestion and the inductively coupled plasma mass spectrometry technique. Serum calcium and total protein and alkaline phosphatase levels were estimated as per standard biochemical techniques. 25-hydroxy vitamin-D
3
, calcitriol, and osteocalcin were estimated using the enzyme-linked immunosorbent assay. In addition to comparative analysis for comparing the two groups, independent linear regression models were explored to investigate the associations between serum calcium and blood lead and osteocalcin levels.
Result
A total of 189 lead-exposed men employed at the lead-smelting plant and 25 male control participants consented to participate. The two groups were similar in age, diet, and body mass index. Occupationally exposed individuals exhibited significantly lower serum calcium and higher bone remodeling markers (osteocalcin and alkaline phosphatase) as compared to controls. However, the serum 25-hydroxy vitamin-D
3
and calcitriol levels were not significantly different between the two groups. Lastly, the serum lead and osteocalcin were weakly but significantly associated with serum calcium levels after controlling for variations in total protein, diet, 25-hydroxy vitamin-D
3
, calcitriol, and alkaline phosphatase in the study participants.
Conclusion
Current observations reinforce the adverse role of lead exposure on calcium metabolism. Although lead exposure is posited to affect calcium metabolism by multiple pathways, current study observations favor the bone remodeling pathway. The observations recommend periodic screening for calcium and bone health among lead-exposed adults.
Graphical abstract
The growing interest in estimating the blood lead levels, for early detection of lead exposure, warranted a need for a validated analytical method for trace levels estimation of lead. The present ...study aimed to develop an analytical method for detecting trace amounts to elevated levels of lead in human blood using the Graphite Furnace Atomic Absorption Spectrometry technique and its application in evaluating blood lead levels among occupationally exposed individuals. The method validation was performed with standard test parameters including linearity, recovery, precision, method detection limit, and limit of quantification. The validation results for each performance parameter were in agreement with acceptable criteria as per standard guidelines. The correlation was observed as optimum linear (R
= 0.998) between absorbance and lead concentration range from 0 to 10 µg/dL. The recoveries for spiked samples ranged between 95 and 105%. The calculated value for the method detection limit was 0.16 µg/dL and the limit of quantification was 0.51 µg/dL. The precision for all spiked concentrations was below 10% of the relative standard deviation. Evaluation of lead exposure among occupationally exposed individuals revealed the study population had found average blood lead level (42.80 ± 12.47 µg/dL), which was above the upper acceptable limit suggested by Occupational Safety and Health Administration, USA. The majority of system-specific symptoms were observed among study groups having mean blood lead levels above 40 µg/dL. However, sociodemographic status and employment factors were found possible determinants of the prevalence of high blood lead levels.
Modulation of initial burst and long term release from electrospun fibrous mats can be achieved by sandwiching the drug loaded mats between hydrophobic layers of fibrous polycaprolactone (PCL). ...Ibuprofen (IBU) loaded PCL fibrous mats (12% PCL-IBU) were sandwiched between fibrous polycaprolactone layers during the process of electrospinning, by varying the polymer concentrations (10% (w/v), 12% (w/v)) and volume of coat (1 ml, 2 ml) in flanking layers. Consequently, 12% PCL-IBU (without sandwich layer) showed burst release of 66.43% on day 1 and cumulative release (%) of 86.08% at the end of 62 days. Whereas, sandwich groups, especially 12% PCLSW-1 & 2 (sandwich layers-1 ml and 2 ml of 12% PCL) showed controlled initial burst and cumulative (%) release compared to 12% PCL-IBU. Moreover, crystallinity (%) and hydrophobicity of the sandwich models imparted control on ibuprofen release from fibrous mats. Further, assay for cytotoxicity and scanning electron microscopic images of cell seeded mats after 5 days showed the mats were not cytotoxic. Nuclear Magnetic Resonance spectroscopic analysis revealed weak interaction between ibuprofen and PCL in nanofibers which favors the release of ibuprofen. These data imply that concentration and volume of coat in flanking layer imparts tighter control on initial burst and long term release of ibuprofen.
Heavy metals are an important group of chemical contaminants and food is the major vehicle for entry into the system. Fish constitute a major source of heavy metals in food. Concentration of heavy ...metals in commercially important species of fish, shellfish and fish products from fish markets in and around the Cochin area was evaluated using an atomic absorption spectrometer. The concentration ranges of Cd, Pb, Hg, Cr, As, Zn, Cu, Co, Mn, Ni, and Se in the samples were <0.07–1, <0.07–1.32, <0.05–2.31, <0.05 to 3.65, <0.1–4.14, 0.6 to 165, 0.15 to 24, <0.02 to 0.85, <0.08 to 9.2, <0.032–1.38 and; <0.03–1.35
mg/kg, respectively. The present study showed that different metals were present in the samples at different levels but within the maximum residual levels prescribed by the EU and USFDA and the fish and shellfish from these areas, in general, are safe for human consumption.
Vehicular emissions on long-term exposure predispose metropolitan bus drivers to cardiorespiratory ailments.
To evaluate the cardiorespiratory risk of urban metropolitan bus drivers related to ...vehicular emission exposure.
Bus drivers (with service >5 years, n = 254) and their administrative controls (primarily engaged in indoor white collared jobs, n = 73) were recruited. Demographic, occupational and clinical details were collected through pre-validated standardized format. Pulmonary Function Test (PFT) and lipid profile were carried out with standard protocol. Risk for cardiovascular events for preceding 10-years was estimated with WHO/ISH risk prediction chart and QRISK3 score. Exposure assessments for particulate matter (PM) were performed for both groups while duty hours.
Exposure of drivers to PM2.5 six times and PM10 five times higher in comparison to administration staff (PM2.5- 970.9 v/s 145.0μg/m3 TWA and PM10- 1111.7 v/s 233.8μg/m3 TWA). Bus drivers exhibited significantly higher prevalence of respiratory symptoms (dyspnea-25% v/s 6.8% and cough-20.1% v/s 9.8%) and compromised PFT (obstructive-21% v/s 5.7% and restrictive-4.2% v/s 2.9%) in comparison to controls. Multivariate regression statistics reveal a significant decline for FEV1/FVC and FEV25-75 % among bus drivers compared to controls, controlling the influence of physiological and environmental factors. The difference between predicted cardiac age and their respective chronological age was twice higher (8.3 v/s 4.3 years) among drivers compared to their administration staff.
Bus drivers were exposed to high levels of outdoor air pollutants. Further, the drivers exhibited higher risk for ischemic attack and obstructive airway diseases as compared to administration staff.
PDF
