Acoustic communication plays an important role in coordinating group dynamics and collective movements across a range of taxa. However, anthropogenic disturbance can inhibit the production or ...reception of acoustic signals. Here, we investigate the effects of noise and light pollution on the calling and collective behaviour of wild jackdaws (
), a highly social corvid species that uses vocalizations to coordinate collective movements at winter roosting sites. Using audio and video monitoring of roosts in areas with differing degrees of urbanization, we evaluate the influence of anthropogenic disturbance on vocalizations and collective movements. We found that when levels of background noise were higher, jackdaws took longer to settle following arrival at the roost in the evening and also called more during the night, suggesting that human disturbance may cause sleep disruption. High levels of overnight calling were, in turn, linked to disruption of vocal consensus decision-making and less cohesive group departures in the morning. These results raise the possibility that, by affecting cognitive and perceptual processes, human activities may interfere with animals' ability to coordinate collective behaviour. Understanding links between anthropogenic disturbance, communication, cognition and collective behaviour must be an important research priority in our increasingly urbanized world. This article is part of the theme issue 'The power of sound: unravelling how acoustic communication shapes group dynamics'.
DNA methylation is strongly associated with smoking status at multiple sites across the genome. Studies have largely been restricted to European origin individuals yet the greatest increase in ...smoking is occurring in low income countries, such as the Indian subcontinent. We determined whether there are differences between South Asians and Europeans in smoking related loci, and if a smoking score, combining all smoking related DNA methylation scores, could differentiate smokers from non-smokers.
Illumina HM450k BeadChip arrays were performed on 192 samples from the Southall And Brent REvisited (SABRE) cohort. Differential methylation in smokers was identified in 29 individual CpG sites at 18 unique loci. Interaction between smoking status and ethnic group was identified at the AHRR locus. Ethnic differences in DNA methylation were identified in non-smokers at two further loci, 6p21.33 and GNG12. With the exception of GFI1 and MYO1G these differences were largely unaffected by adjustment for cell composition. A smoking score based on methylation profile was constructed. Current smokers were identified with 100% sensitivity and 97% specificity in Europeans and with 80% sensitivity and 95% specificity in South Asians.
Differences in ethnic groups were identified in both single CpG sites and combined smoking score. The smoking score is a valuable tool for identification of true current smoking behaviour. Explanations for ethnic differences in DNA methylation in association with smoking may provide valuable clues to disease pathways.
Medulloblastoma is a malignant childhood cerebellar tumour type that comprises distinct molecular subgroups. Whereas genomic characteristics of these subgroups are well defined, the extent to which ...cellular diversity underlies their divergent biology and clinical behaviour remains largely unexplored. Here we used single-cell transcriptomics to investigate intra- and intertumoral heterogeneity in 25 medulloblastomas spanning all molecular subgroups. WNT, SHH and Group 3 tumours comprised subgroup-specific undifferentiated and differentiated neuronal-like malignant populations, whereas Group 4 tumours consisted exclusively of differentiated neuronal-like neoplastic cells. SHH tumours closely resembled granule neurons of varying differentiation states that correlated with patient age. Group 3 and Group 4 tumours exhibited a developmental trajectory from primitive progenitor-like to more mature neuronal-like cells, the relative proportions of which distinguished these subgroups. Cross-species transcriptomics defined distinct glutamatergic populations as putative cells-of-origin for SHH and Group 4 subtypes. Collectively, these data provide insights into the cellular and developmental states underlying subtype-specific medulloblastoma biology.
AbstractThe alignment of curriculum content with standards is a quality improvement measure used to identify gaps or overlaps in academic content. Curriculum mapping results, while commonly used, are ...rarely reported. Undergraduate faculty mapped 11 courses in a single timepoint using a web-based curriculum mapping tool. Quantitative results were calculated and compared to defined client needs categories. A validation analysis was performed by mapping one course in triplicate. Faculty feedback following the exercise was obtained by online survey. Three client needs categories were determined to be marginally out of range in the initial analysis of the quantitative mapping data. The curriculum map was substantiated by high correlation of curriculum measures in the validation process. A curriculum map should be planned with the aim as the central guide to inform the standards and measures selected for comparison. Measures and Standards chosen for the map as well as any actionable data generated should be informed by the aim. Validation can be performed by mapping subsets of the curriculum in triplicate. A team approach with high participation from the faculty should be considered when planning a full curriculum map.
M and M-like surface proteins from group A Streptococcus (GAS) act as virulence factors and have been used in multiple vaccine candidates. While the M protein has been extensively studied, the two ...genetically and functionally related M-like proteins, Mrp and Enn, although present in most streptococcal strains have been relatively less characterised. We compile the current state of knowledge for these two proteins, from discovery to recent studies on function and immunogenicity, using the M protein for comparison as a prototype of this family of proteins. We focus on the known interactions between M-like proteins and host ligand proteins, and analyse the genetic data supporting these interactions. We discuss known and possible functions of M-like proteins during GAS infections, and highlight knowledge gaps where further investigation is warranted.
From mouse to primate, there is a striking discontinuity in our current understanding of the neural coding of motion direction. In non-primate mammals, directionally selective cell types and circuits ...are a signature feature of the retina, situated at the earliest stage of the visual process. In primates, by contrast, direction selectivity is a hallmark of motion processing areas in visual cortex, but has not been found in the retina, despite significant effort. Here we combined functional recordings of light-evoked responses and connectomic reconstruction to identify diverse direction-selective cell types in the macaque monkey retina with distinctive physiological properties and synaptic motifs. This circuitry includes an ON-OFF ganglion cell type, a spiking, ON-OFF polyaxonal amacrine cell and the starburst amacrine cell, all of which show direction selectivity. Moreover, we discovered that macaque starburst cells possess a strong, non-GABAergic, antagonistic surround mediated by input from excitatory bipolar cells that is critical for the generation of radial motion sensitivity in these cells. Our findings open a door to investigation of a precortical circuitry that computes motion direction in the primate visual system.
Research Summary
As a policy response to historically strained police–minority community relations, police diversification is hoped to improve policing outcomes in minority communities. An improved ...police–community relationship may be expected to lead to increased citizen cooperation in crime investigations and therefore be beneficial for crime clearance, but there are different perspectives on the nature of this link. We examined several measures of Black police representation (Black officer percentage, Black racial congruence between the police force and the jurisdiction's population, and presence of Black police chief or head) and their relationships with arrest clearance of National Incident‐Based Reporting System aggravated assault incidents involving Black victims in 205 agencies. We also explored similar Hispanic police representation measures in a sample of aggravated assault incidents with Hispanic victims in 158 agencies. In multilevel survival analyses with agency/jurisdiction‐ and incident‐level controls, none of the Black representation measures were significantly associated with clearance of Black victims’ incidents. We obtained similar results for the Hispanic representation measures and clearance of Hispanic victims’ incidents.
Policy Implications
Although the findings do not indicate a benefit of diversity for clearance involving minority victims, it is encouraging that neither do results suggest that increasing minority police representation would harm clearance. Responding to the public's strong call for demographic reform, it appears that police agencies can continue to increase their racial and ethnic diversity without sacrificing clearance rates, a main indicator of police performance. At least for clearance, warnings of reduced performance when equitable representation is an agency priority do not seem to be well‐founded. There should be continuing research on policing outcomes and diversity as U.S. agencies increase their minority representation, and agencies should emphasize the recruitment, hiring, and retention of minority officers.
Abstract
Cholinergic circuits in the central nervous system are vulnerable to age-related functional decline, but it is not known if aging impacts cholinergic signaling in the vestibular sensory ...organs, which are critically important to balance maintenance and visual gaze stability. We have previously shown cholinergic neurotransmission between vestibular efferent terminals and type II mechanosensory hair cells requires the alpha9 (Chrna9) nicotinic receptor subunit. Homozygous knockout of the alpha9 subunit causes vestibulo-ocular reflex adaptation deficits that mirror those observed in aged mice. This prompted examination of cholinergic signaling in the vestibular sensory organs of aged mice. We confirmed older (>24 months) mice had impaired performance in a balance beam task compared to young (3–4 months) adult mice. While there was no qualitative loss of cholinergic axon varicosities in the crista ampullaris of old mice, qPCR analysis revealed reduced expression of nicotinic receptor subunit genes Chrna1, Chrna9, and Chrna10 in the cristae of old relative to young mice. Functionally, single-cell patch clamp recordings taken from type II vestibular hair cells exposed to acetylcholine show reduced conductance through alpha9/10 subunit-containing nicotinic receptors in older mice, despite preserved passive membrane properties and voltage-activated conductances. These findings suggest that cholinergic signaling in the peripheral vestibular sensory organs is vulnerable to aging processes, manifesting in dynamic molecular and functional age-related changes. Given the importance of these organs to our everyday activities, and the dramatic increase in fall incidence in the older, further investigation into the mechanisms of altered peripheral vestibular function in older humans is warranted.
Graphical Abstract
Graphical Abstract
Abstract Individuals with Down syndrome, the genetic condition caused by trisomy 21, exhibit strong inter-individual variability in terms of developmental phenotypes and diagnosis of co-occurring ...conditions. The mechanisms underlying this variable developmental and clinical presentation await elucidation. We report an investigation of human chromosome 21 gene overexpression in hundreds of research participants with Down syndrome, which led to the identification of two major subsets of co-expressed genes. Using clustering analyses, we identified three main molecular subtypes of trisomy 21, based on differential overexpression patterns of chromosome 21 genes. We subsequently performed multiomics comparative analyses among subtypes using whole blood transcriptomes, plasma proteomes and metabolomes, and immune cell profiles. These efforts revealed strong heterogeneity in dysregulation of key pathophysiological processes across the three subtypes, underscored by differential multiomics signatures related to inflammation, immunity, cell growth and proliferation, and metabolism. We also observed distinct patterns of immune cell changes across subtypes. These findings provide insights into the molecular heterogeneity of trisomy 21 and lay the foundation for the development of personalized medicine approaches for the clinical management of Down syndrome.
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