Abstract
The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane ...protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6
194–213
of AQP6 loop C contribute to intracellular endoplasmic reticulum (ER) retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous expression systems.
This paper presents results of a research in the field of software development, design methods, as well as training and synthesis of binary neural networks. The research is based on the model of a ...biomorphic neuron proposed by A.A. Zhdanov, which features noise immunity and is capable of forgetting and additional training. Software tools for designing and visualizing the modeling of binary neural structures are described. Use cases and features of a formal markup language for neural network models, as well as principles of generating deep learning structures, are discussed. A neural network markup language interpreter can automatically generate source code in Verilog with the description of the neural-like implementation of intelligent systems for software and hardware solutions on programmable logic devices (PLDs).
Cognitive ergonomics of DNA-algorithms Stepanyan, I V
IOP conference series. Materials Science and Engineering,
04/2021, Letnik:
1129, Številka:
1
Journal Article
Recenzirano
Odprti dostop
Abstract
To improve the ergonomic qualities of graphical interfaces and optimize the visual perception of large data presented in text form, a method of information processing based on DNA algorithms ...is proposed. These algorithms were developed by the author in the study of the molecular genetic and structural properties of long nucleotide sequences. The result of processing information presented in text form is an object of discrete geometry, two-dimensional projections of which can serve for visual identification of the internal structure of information, as well as for interpretation as characteristic symbols. At the same time, the considered transformations have the property of visualizing the characteristic cluster structure of information. It is shown that genetic nucleotide sequences, as well as texts in natural language, lend themselves to visual classification, which contributes to an increase in the productivity of mental work when analyzing big data. It is shown that DNA algorithms can reduce the load on the visual analyzer during the initial examination of data presented in text form. This makes it possible to apply this method when operators interact with various information streams and text data of arbitrary nature, as well as to improve the ergonomic qualities of specialized graphical interfaces.
DNA Clustering Algorithms Stepanyan, I. V.
Automatic documentation and mathematical linguistics,
2021/1, Letnik:
55, Številka:
1
Journal Article
Recenzirano
This paper makes two generalizations of the previously presented algorithms of the author based on the principles of information coding in molecular genetics. This is an account of the frequency ...characteristics of subalphabetic representations of polynucleotides and a generalization of an algorithm for processing arbitrary information presented in a quaternary code. The second generalization indicates the general significance of the proposed algorithms, which the author called molecular genetic or DNA algorithms, emphasizing their difference from the well-known genetic algorithms of the Holland type. An example of displaying the results of the operation of DNA algorithms in the frequency domain with visualization of the cluster structure is given. The example makes it possible to trace a structure that is quite common for DNA, which consists of a main cluster and several satellite clusters. Natural language texts processed by DNA algorithms in the structural and frequency domains are analyzed and compared.
Adriane Fugh-Berman and colleagues describe how strategies similar to those used to market drugs to physicians are directed towards people with hemophilia.
Gastrointestinal motility patterns can be mapped
via
electrical signals measured non-invasively on the body surface. However, short-term (≈ 2–4 h) meal response studies as well as long-term ...monitoring (≥ 24 h) may be hindered by skin irritation inherent with traditional Ag/AgCl pre-gelled (“wet”) electrodes. The aim of this work was to investigate the practical utility of using dry electrodes for GI body-surface electrical measurements. To directly compare dry vs. wet electrodes, we simultaneously recorded electrical signals from both types arranged in a 9-electrode array during an ≈ 2.5 h colonic meal-response study. Wavelet-based analyses were used to identify the signature post-meal colonic cyclic motor patterns. Blinded comparison of signal quality was carried out by four expert manual reviewers in order to assess the practical utility of each electrode type for identifying GI activity patterns. Dry electrodes recorded high-quality GI signals with signal-to-noise ratio of 10.0 ± 3.5 dB, comparable to that of wet electrodes (9.9 ± 3.6 dB). Although users rated dry electrodes as slightly more difficult to self-apply, they caused no skin irritation and were thus better tolerated overall. Dry electrodes are a more comfortable alternative to conventional wet electrode systems, and may offer a potentially viable option for long-term GI monitoring studies.
The pandemic and social upheaval of 2020 and 2021 have given language instructors the mission—and obligation—of adapting their curriculum to multiple crises. Although these crises have abated ...somewhat with classes returning to campus, we will always face fluctuations in terms of the events affecting our language regions and communities. How can we address current affairs in our classes with as much plasticity as possible? This article discusses the use of open integrated performance assessments, students’ attitudes toward these assessments, and offers a model for creating materials that respond to the ACTFL standards while also taking into account the issues of the day.
The effects of lutein (100 and 200
μg/g muscle), sesamol (250 and 500
μg/g muscle), ellagic acid (300 and 600
μg/g muscle) and olive leaf extract (100 and 200
μg/g muscle) on total viable counts ...(TVCs), lipid oxidation (thiobarbituric acid-reactive substances, TBARS), colour, oxymyoglobin oxidation, pH, water-holding capacity (WHC), sensorial properties of raw beef patties (
M. longissimus thoracis et lumborum) stored in modified atmosphere packs (80% O
2:20% CO
2) (MAP) aerobically at 4
°C for up to 8 and 12
days, respectively, were examined. All the nutraceuticals reduced (
P
<
0.001) TVCs. The addition of sesamol, ellagic acid and olive leaf extract reduced (
P
<
0.001) TBARS in raw beef patties in both packaging systems. Sesamol addition to beef resulted in lower (
P
<
0.01)
a* redness values and increased oxymyoglobin oxidation. Conversely, lutein and olive leaf extract reduced (
P
<
0.001) oxymyoglobin oxidation relative to the control. The graded addition of ellagic acid and olive leaf extract improved (
P
<
0.001) WHC.
This work aims to provide a better understanding of the cumulative effect of successive nanosecond repetitively pulsed (NRP) discharges on the ignition process. Fast chemiluminescence imaging of both ...the post-discharge and the following flame was used to analyze the ignition of a lean propane/air mixture (ϕ = 0.7) by a train of 82 NRP discharges at a pulse repetition frequency (PRF) of 30 kHz, in comparison with the traditional spark ignition. The transition from the non-equilibrium plasma to the ignition kernel has been imaged. It has been found that, differently from traditional spark ignition and despite similar experimental conditions, each NRP ignition develops in a unique way. NRP discharges generate both highly reactive species and thermal instabilities in the gap: the multi-pulse strategy produce a gas motion resulting in a jetting phenomenon, reported here for the first time. An algorithm within ImageJ software was used to quantitatively describe the observed jetting phenomenon. The results presented in this work suggest that jetting is the driver of the ignition initiated by NRP discharges, at least in the experimental conditions investigated here.
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