Long noncoding RNA HOXA11 antisense RNA (HOXA11-AS) is involved in tumorigenesis and development of some human cancers. However, the role of HOXA11-AS in human laryngeal squamous cell cancer (LSCC) ...is yet unclear. In this study, we firstly investigated the expression of HOXA11-AS in LSCC. Microarray and qRT-PCR showed that the level of HOXA11-AS was significantly higher in LSCC than that in the corresponding adjacent non-neoplastic tissues. ISH revealed that HOXA11-AS was strongly expressed in the nucleus and closely related to the T grade, neck nodal metastasis, and clinical stage. Patients with T3-4 grade, neck nodal metastasis, or advanced clinical stage presented a high HOXA11-AS expression. Kaplan-Meier analysis showed that high HOXA11-AS expression could predict a poor prognosis in LSCC patients. Furthermore, HOXA11-AS knockdown significantly inhibited the growth, migration, and invasion of LSCC cells. Taken together, the current data indicated that HOXA11-AS plays an oncogenic role in the cellular processes of LSCC and serve as a novel marker and a potential therapeutic target in LSCC patients.
Approximately 40% of patients with Henoch–Schonlein purpura (HSP) develop Henoch–Schonlein purpura nephritis (HSPN) after 4 to 6 weeks of subcutaneous hemorrhaging. Immunoglobulin-A nephropathy ...(IgAN) and HSPN have numerous similarities, which can cause difficulty in correctly diagnosing the disorder during a differential diagnosis. The pathogenesis of the 2 diseases is not clear. We enrolled 137 patients with HSPN, 107 patients with IgAN, and 28 healthy (control) patients in our study. The levels of alpha-smooth muscle actin (α-SMA), c-Met, and Gal-deficient IgA1 (Gd-IgA1) in the 3 patient groups were determined and compared. The α-SMA, c-Met, and Gd-IgA1 levels and the clinical data from the patients with HSPN were analyzed for any correlations. The α-SMA and c-Met levels of the HSPN group were significantly higher than those of the IgAN and healthy control groups ( P < 0.01). The Gd-IgA1 levels of the HSPN and IgAN groups were significantly different from the Gd-IgA1 level of the healthy control group ( P < 0.01). The α-SMA levels of the HSPN group were positively correlated with blood urea nitrogen levels, serum creatinine levels, hematuria index, and proteinuria levels ( P < 0.01). The c-Met levels of the HSPN group were positively correlated with the blood urea nitrogen and serum creatinine levels ( P < 0.01). There were no significant differences among the α-SMA, c-Met, and Gd-IgA1 levels or the clinical data for the child and adult patients with HSPN. The serum levels of α-SMA and c-Met in patients with HSPN may be associated with the degree of disease severity. Gd-IgA1 is involved in the common immunologic pathogenesis of HSPN and IgAN.
β-Ionone is a constituent of vegetables and fruits, and can induce apoptosis in some types of malignant cells. However, the mechanism of apoptosis in osteosarcoma (U2os) cells is currently unclear. ...In this study, we determined whether β-ionone can induce apoptosis in U2os cells in vitro and which signal pathway(s) is involved. We found that β-ionone inhibited cell proliferation in U2os cells in a concentration- and time-dependent manner and caused cell cycle arrest at the G1-S phase. TUNEL assay, DNA ladder and assessment of Caspase 3 activity showed that apoptosis was the determinant in the effects of β-ionone. Furthermore, Expression of the p53 protein increased in a concentration-dependent and time-dependent manner according to immunocytochemistry and immunoblotting after β-ionone treatment. In addition, β-ionone upregulated Bax protein and downregulated Bcl2 protein which led to Bax translocation and cytochrome c release, subsequently activated Caspase 3, thus resulting in apoptosis. In summary, these data suggested that β-ionone induced apoptosis in a concentration-dependent manner in U2os cells via a p53-dependent mitochondrial pathway.
beta-Ionone is a constituent of vegetables and fruits, and can induce apoptosis in some types of malignant cells. However, the mechanism of apoptosis in osteosarcoma (U2os) cells is currently ...unclear. In this study, we determined whether beta-ionone can induce apoptosis in U2os cells in vitro and which signal pathway(s) is involved. We found that beta-ionone inhibited cell proliferation in U2os cells in a concentration- and time-dependent manner and caused cell cycle arrest at the G1-S phase. TUNEL assay, DNA ladder and assessment of Caspase 3 activity showed that apoptosis was the determinant in the effects of beta-ionone. Furthermore, Expression of the p53 protein increased in a concentration-dependent and time-dependent manner according to immunocytochemistry and immunoblotting after beta-ionone treatment. In addition, beta-ionone upregulated Bax protein and downregulated Bcl2 protein which led to Bax translocation and cytochrome c release, subsequently activated Caspase 3, thus resulting in apoptosis. In summary, these data suggested that beta-ionone induced apoptosis in a concentration-dependent manner in U2os cells via a p53-dependent mitochondrial pathway. PUBLICATION ABSTRACT
b-Ionone is a constituent of vegetables and fruits, and can induce apoptosis in some types of malignant cells. However, the mechanism of apoptosis in osteosarcoma (U2os) cells is currently unclear. ...In this study, we determined whether b-ionone can induce apoptosis in U2os cells in vitro and which signal pathway(s) is involved. We found that b-ionone inhibited cell proliferation in U2os cells in a concentration- and time-dependent manner and caused cell cycle arrest at the G1-S phase. TUNEL assay, DNA ladder and assessment of Caspase 3 activity showed that apoptosis was the determinant in the effects of b-ionone. Furthermore, Expression of the p53 protein increased in a concentration-dependent and time-dependent manner according to immunocytochemistry and immunoblotting after b-ionone treatment. In addition, b-ionone upregulated Bax protein and downregulated Bcl2 protein which led to Bax translocation and cytochrome c release, subsequently activated Caspase 3, thus resulting in apoptosis. In summary, these data suggested that b-ionone induced apoptosis in a concentration-dependent manner in U2os cells via a p53-dependent mitochondrial pathway.
High-performance Ag–Cu alloy nanoparticles (NPs) were successfully synthesized by a solventless mix–bake–wash method and tested for NH3-SCO. The prepared Ag2Cu1 catalyst with a perfect Ag–Cu alloy ...structure exhibited better T 100 (200 °C, the temperature at which 100% NH3 conversion was obtained), higher reaction rates, and lower E a compared to that with ordinary bimetallic Ag–Cu (AgCuO x ). The characterization data revealed much smaller Ag–Cu alloy nanoparticles of the Ag2Cu1 catalyst and more Ag/Cu metallic species on the surface, which can increase the amount of chemisorbed surface oxygen (Oβ) and enhance NH3 adsorption and activation in the low-temperature range, therefore leading to a much higher NH3-SCO activity. Kinetic studies and density functional theory calculations indicated that Cu decoration at Ag by Ag–Cu alloying could enhance the adsorption/activation of NH3 and O2. It has been found that O2 was more easily transformed from the adsorption state to the transition state than NH3, which enhanced the performance of NH3 oxidation. In addition, the Ag2Cu1 catalyst exhibited excellent durability because of the stabilization of Ag sites by the Ag–Cu alloy structure.
The oncogene protein ubiquitin-conjugating enzyme E2T (UBE2T) is reported to be upregulated in hepatocellular carcinoma (HCC) and correlated with poor clinical outcomes of HCC patients. However, the ...underlying mechanism by which UBE2T exerts its oncogenic function in HCC remains largely unexplored. In this study, in vitro and in vivo experiments suggested that UBE2T promoted HCC development including proliferation and metastasis. GSEA analysis indicated that UBE2T was positively correlated with pyrimidine metabolism, and LC/MS-MS metabolomics profiling revealed that the key products of pyrimidine metabolism were significantly increased in UBE2T-overexpressing cells. UBE2T overexpression led to the upregulation of several key enzymes catalyzing de novo pyrimidine synthesis, including CAD, DHODH, and UMPS. Moreover, the utilization of leflunomide, a clinically approved DHODH inhibitor, blocked the effect of UBE2T in promoting HCC progression. Mechanistically, UBE2T increased Akt K63-mediated ubiquitination and Akt/β-catenin signaling pathway activation. The disruption of UBE2T-mediated ubiquitination on Akt, including E2-enzyme-deficient mutation (C86A) of UBE2T and ubiquitination-site-deficient mutation (K8/14 R) of Akt impaired UBE2T's effect in upregulating CAD, DHODH, and UMPS. Importantly, we demonstrated that UBE2T was positively correlated with p-Akt, β-catenin, CAD, DHODH, and UMPS in HCC tumor tissues. In summary, our study indicates that UBE2T increases pyrimidine metabolism by promoting Akt K63-linked ubiquitination, thus contributing to HCC development. This work provides a novel insight into HCC development and a potential therapeutic strategy for HCC patients.
Aiming to solve the problems of system sum-rate reduction caused by wavefront distortion, this paper studies an adaptive anti-jamming beamforming through a preprocessing deep reinforcement learning ...(DRL). This algorithm combines the perception ability of deep learning (DL) with the decision-making ability of reinforcement learning (RL). First, a global positioning system (GPS) signal model is designed by using an dual-polarized antenna array and a intelligent reflecting surface (IRS), where IRS is designed to enhance the transmitting power while providing the double degrees of freedom. Second, a preprocessing DL network based on a cyclic convolutional neural network (CNN) is built to approximate the evaluated variable to the target value. Then an DRL anti-jamming strategy is proposed to delete the wavefront distortion in dynamic jamming environment, which is beneficial to improve the system sum-rate. Simulations indicate the GPS antenna array has the symmetrical “apple-shape” omnidirectional radiation, and the side-lobe of beamforming is lower than − 15 dB, null-depth has − 33 dB at θ = − 50°, the system sum-rate from 15 to 70 bits/s/Hz are obtained when SNR = 10–45 dB. Additionally, CNN with loss = − 25 dB when slot > 220, then CNN has a good preprocessing performance.
A type of silsesquioxane-based flexible polyimide aerogel was synthesized by 3,3′,4,4′-benzophenonetetracarboxylic dianhydride (BTDA), 4,4′-diaminodiphenyl and octa(aminopropylsilsesquioxane) ...(POSS-NH
2
) with a facile thermal curing and atmospheric drying process. POSS-NH
2
was introduced into the polyimide backbone as a cross-linking agent. The obtained aerogel possessed high hydrophobicity with a water contact angle exceeding 143°. And the aerogels showed good cyclic adsorption capacity for various organic solvents, such as acetone, methanol, ethyl acetate, etc. After 15 adsorption experiments, the original adsorption capacity of the aerogels (more than 3 times their own weight) was still maintained. SEM and BET confirmed that the introduction of POSS-NH
2
could form a larger pore structure inside the aerogel, thereby achieving an improvement in adsorption performance. The results indicated this kind of aerogel might be flexibly utilized as adsorbent materials for oil pollutants and harmful organic reagents in the field of wastewater treatment.
Graphic abstract
PDF
