Objectives and Background
The involvement of DNA methylation in periodontal disease is not clear. Lipopolysaccharide (LPS) derived from Porphyromonas gingivalis is involved in the progression of ...periodontal disease. We recently developed an in vitro model of LPS infection in human periodontal fibroblast cells (HPdLFs) for a prolonged period. In this study, we examined genome‐wide analysis of DNA methylation in HPdLFs stimulated with LPS derived from P. gingivalis for a prolonged period. We noted the hypermethylation of extracellular matrix (ECM)‐related genes and examined whether hypermethylation affected their transcription levels.
Material and Methods
HPdLFs were grown in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. The culture was repeated, alternating 3 d with LPS derived from P. gingivalis and 3 d without LPS for 1 mo. Untreated samples were used as controls. DNA was analyzed using the human CpG island microarray. Quantitative methylation‐specific polymerase chain reaction was carried out to confirm reproducibility of the microarray data. The expression levels of mRNA of the selected ECM‐related genes from the data were analyzed by quantitative reverse transcription–polymerase chain reaction.
Results
We found 25 ECM‐related genes with hypermethylation at the CpG island of the promoter region, which exhibited a fourfold greater hypermethylation than controls. Among these genes, hypermethylation of nine ECM‐related genes, FANK1, COL4A1‐A2, 12A1 and 15A1, LAMA5 and B1, MMP25, POMT1 and EMILIN3, induced a significantly downregulated expression of their mRNA.
Conclusion
These results indicate that LPS derived from P. gingivalis may cause DNA hypermethylation of some ECM‐related genes followed by downregulated expression of their transcriptional levels.
We describe the details of a silicon–tungsten prototype electromagnetic calorimeter module and associated readout electronics. Detector performance for this prototype has been measured in test beam ...experiments at the CERN PS and SPS accelerator facilities in 2015/16. The results are compared to those in Monte Carlo Geant4 simulations. This is the first real-world demonstration of the performance of a custom ASIC designed for fast, lower-power, high-granularity applications.
(
) gene has been implicated in X-linked intellectual disability (XLID), but the molecular pathogenesis is unknown. We show that Ftsj1 is responsible for 2'-
-methylation of 11 species of cytosolic ...transfer RNAs (tRNAs) at the anticodon region, and these modifications are abolished in Ftsj1 knockout (KO) mice and XLID patient-derived cells. Loss of 2'-
-methylation in Ftsj1 KO mouse selectively reduced the steady-state level of tRNA
in the brain, resulting in a slow decoding at Phe codons. Ribosome profiling showed that translation efficiency is significantly reduced in a subset of genes that need to be efficiently translated to support synaptic organization and functions. Ftsj1 KO mice display immature synaptic morphology and aberrant synaptic plasticity, which are associated with anxiety-like and memory deficits. The data illuminate a fundamental role of tRNA modification in the brain through regulation of translation efficiency and provide mechanistic insights into FTSJ1-related XLID.
The baryon production mechanism at the intermediate pT (2–5 GeV/c) at RHIC is still not well understood. The beam energy scan data in Cu+Cu and Au+Au systems at RHIC may provide us a further insight ...on the origin of the baryon anomaly and its evolution as a function of . In 2005 RHIC physics program, the PHENIX experiment accumulated the first intensive low beam energy data in Cu+Cu collisions. We present the preliminary results of identified charged hadron spectra in Cu+Cu at and 62.4 GeV using the PHENIX detector. The centrality and beam energy dependences of (anti)proton to pion ratios and the nuclear modification factors for charged pions and (anti)protons are presented.
The study aimed for the complete purification and recharacterization of the highly hydrophobic circular bacteriocins, gassericin A and reutericin 6. Gassericin A and reutericin 6 were purified to ...homogeneity using previously described method and reverse-phase HPLC with an octyl column and eluents of aqueous acetonitrile and 2-propanol. Mass analysis, N-terminal sequencing and bacteriocin assay of the HPLC-purified bacteriocins showed the two bacteriocins had identical seamless circular structures with the same m/z value (5651) of M + H⁺ and both had the same specific activity. d/l-amino acid composition analysis using two distinct methods with the chiral fluorescent derivatization reagents (+)-1-(9-fluorenyl)ethyl chloroformate and o-phthalaldehyde/N-acetyl- l-cystein revealed neither gassericin A nor reutericin 6 contained d-alanine residues contrary to our previous results. Purified gassericin A and reutericin 6 are chemically identical circular molecules containing no d-alanine residues. The HPLC conditions developed in this study will facilitate advanced purification and correct characterization of other highly hydrophobic bacteriocins.
A reaction plane detector for PHENIX at RHIC Richardson, E.; Akiba, Y.; Anderson, N. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
04/2011, Letnik:
636, Številka:
1
Journal Article
Recenzirano
Odprti dostop
A plastic scintillator paddle detector with embedded fiber light guides and photomultiplier tube readout, referred to as the Reaction Plane Detector (RXNP), was designed and installed in the PHENIX ...experiment prior to the 2007 run of the Relativistic Heavy Ion Collider (RHIC). The RXNP's design is optimized to accurately measure the reaction plane (RP) angle of heavy-ion collisions, where, for mid-central sNN=200GeV Au+Au collisions, it achieved a 2nd harmonic RP resolution of ∼0.75, which is a factor of ∼2 greater than PHENIX's previous capabilities. This improvement was accomplished by locating the RXNP in the central region of the PHENIX experiment, where, due to its large coverage in pseudorapidity (1.0<|η|<2.8) and ϕ(2π), it is exposed to the high particle multiplicities needed for an accurate RP measurement. To enhance the observed signal, a 2-cm Pb converter is located between the nominal collision region and the scintillator paddles, allowing neutral particles produced in the heavy-ion collisions to contribute to the signal through conversion electrons. This paper discusses the design, operation and performance of the RXNP during the 2007 RHIC run.
Recent results on identified hadrons from the PHENIX experiment in Au+Au collisions at mid-rapidity at √
s
NN
= 200 GeV are presented. The centrality dependence of transverse momentum distributions ...and particle ratios for identified charged hadrons are studied.
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