New experimental results on in-beam gamma spectroscopy of neutron rich boron isotopes are presented for 17B and its neutron removal fragments 14,15B, after scattering with a H2 target. A gamma ...transition for 17B is observed at 1089±15 keV. The fragment 15B is observed abundantly associated with a gamma transition of 1336±10 keV. This suggests for the first time a core-excited structure for 17B thereby providing a new insight into its structure. Observations for 12,14B are also presented. The data set provides a useful systematic study of first excited states of neutron rich boron isotopes showing the dramatic drop in excitation energy beyond N=8.
Abstract
Background
A suicidal function of neutrophils to ensnare and kill microbes by expelling neutrophil extracellular traps (NETs) via histone citrullination and chromatin decondensation has ...emerged as it enhances local inflammation and thrombogenicity at the culprit lesion of acute myocardial infarction (AMI).
Purpose
This multicenter study was aimed to examine 1) the histopathological feature of coronary thrombus with the NETs formation in AMI patients, and 2) the association of NETs with impaired coronary flow after percutaneous coronary intervention (PCI) for AMI patients.
Methods
Coronary thrombi were obtained from 44 patients with AMI male; 77%, age; 69±12(SD) y-o)who underwent PCI within 24 hours from the onset. Aspirated thrombi were immediately bisected and frozen, and 5-µm thickness unstained sections were processed for polarized light microscopy (PLM) imaging and immunofluorescence staining. NETs were immunohistochemically defined when objects were co-stained with anti-myeloperoxidase and anti-citrullinated histone H3 antibodies (Figure A,B). Adjacent sections were stained with Masson’s trichrome to measure the whole thrombus area. The ImageJ1 software was utilized for the automatic segmentation on the digitized histology images. Segmented NETs area was divided by the thrombus area as a measure of NETs. Clinical characteristics, including AMI subset, post PCI final TIMI flow grade and TIMI frame count, were collected.
Results
All aspirated thrombi exhibited NETs (Figure A), and then subjects were split into 2 groups with a median value of NETs (62.8 x10-4 um2/um2); high-NETs and low-NETs groups. Patient characteristics were all comparable between the 2 groups. PLM results showed that birefringent crystals, suggestive of cholesterol crystals, were more frequently noted in the thrombi with high-NETs compared with those with low-NETs (50.0% vs. 13.6%, P=0.010). In the immunofluorescence, NETs were seen in the proximity of the crystals (Figure B). The multivariable analysis with covariates of age, sex, major coronary risk factors, MI related to left anterior descending artery and the onset to aspiration time revealed that the presence of birefringent crystals remained an independent predictor for high-NETs formation OR=5.78, 95%CI=1.13-29.66, P=0.035. In the high-NETs group, the prevalence of the final TIMI flow grade 3 was significantly lower than the low-NETs group (63.6% vs. 90.9 %, P=0.031). Likewise, there was a significant positive correlation between NETs area and TIMI frame count, defined as the number of cineframes required for contrast to reach a standardized distal coronary landmark (R=0.681, P<0.001).
Conclusion
The present study demonstrated that NETs were highly expressed in coronary thrombus aspirated from AMI patients in association with crystalline components, relating to coronary flow impairment following PCI in AMI patients.
Lifetime measurements were performed on low-lying excited states of the neutron-rich isotope C17 using the recoil shadow method. The γ-decay mean lifetimes were determined to be 583±21(stat)±35(syst) ...ps for the first excited state at 212 keV and 18.9±0.6(stat)±4.7(syst) ps for the second excited state at 333 keV. Based on a comparison with the empirical upper limits for the electromagnetic transition strengths, these decays are concluded to be predominantly M1 transitions. The reduced M1 transition probabilities to the ground state were deduced to be (1.0±0.1)×10−2μN2 and (8.2−1.8+3.2)×10−2μN2, respectively, for the first and second excited states. The strongly hindered M1 strength as well as the lowered excitation energy represents unique nature of the 212-keV state.
Recent molecular evidence suggests an association with a new herpesvirus, Kaposi’s sarcoma-associated herpesvirus (KSHV/HHV - 8), and primary effusion lymphomas (PELs). PELs have a characteristic ...morphology, phenotype, and clinical presentation, with malignant effusions in the absence of a contiguous solid tumor mass. We have established a cell line (KS - 1) from a KSHV-positive human immunodeficiency virus (HlV)-negative patient with pleural cavity-based lymphoma that was passaged into triple-immunodeficient BNX mice. In contrast to cell lines from body cavity-based lymphomas derived from HIV-positive individuals that contain both KSHV and Epstein Barr viral genome, these cells contain only KSHV, allowing for uncontaminated virologic studies. Ultrastructural examination identified malignant cells with features of late differentiating B cells (immunoblasts). Cells with viral cytopathic effect contained typical 110-nm intranuclear herpesvirus nucleocapsids and complete cytoplasmic virions, confirming the association of PEL with KSHV.
Previously we developed a carcinogenesis model involving the combination of 9, 10-dimethyl-1, 2-benzanthracene (DMBA) application with physical wounding of hamster lingual mucosa. The presence of a ...novel hamster oral papillomavirus (HOPV) was demonstrated and its genome sequenced. In the present study, this HOPV hamster model was used to test whether vaccination with the L1 gene could prevent the development of oral carcinoma. DNA plasmids encoding the L1 gene or the vector alone were injected intramuscularly into 20 vaccinated and 20 control hamsters, respectively. The lingual tips of the hamsters were painted with DMBA for 8 weeks. A portion of the lingual tips was excised, and the tips were then painted daily with DMBA until the animals were killed 13 days later. All control hamsters developed lingual carcinoma, whereas 12 of the L1-vaccinated hamsters showed no lesions. These results suggest that immunization with L1 DNA vaccines may prevent the development of papillomavirus-associated oral cancer.
Ion channels are located in plasma membranes as well as on mitochondrial, lysosomal, and endoplasmic reticulum membranes. They play a critical role in physiology and drug targeting. It is ...particularly challenging to measure the current mediated by ion channels in the lysosomal and the endoplasmic reticulum membranes using the conventional patch clamp method. In this study, we show that our proposed device is applicable for an electrophysiological measurement of various types of ion channel in plasma and organelle membranes. We designed an on-chip device that can form multiple electrical contacts with a measurement system when placed on a mount system. Using crude cell membranes containing ion channels extracted from cultured cells without detergents, we detected open/close signals of the hERG, TRPV1, and NMDA channels on plasma membranes, those of the TRPML1 channels on lysosomal membranes, and open/close signals of the RyR channels on SR membranes. This method will provide a highly versatile drug screening system for ion channels expressed by various cell membranes, including plasma, SR, mitochondrial, Golgi, and lysosomal membranes.
Among the four groups of HIV-1 (M, N, O, and P), HIV-1M alone is pandemic and has rapidly expanded across the world. However, why HIV-1M has caused a devastating pandemic while the other groups ...remain contained is unclear. Interestingly, only HIV-1M Vpu, a viral protein, can robustly counteract human tetherin, which tethers budding virions. Therefore, we hypothesize that this property of HIV-1M Vpu facilitates human-to-human viral transmission. Adopting a multilayered experimental-mathematical approach, we demonstrate that HIV-1M Vpu confers a 2.38-fold increase in the prevalence of HIV-1 transmission. When Vpu activity is lost, protected human populations emerge (i.e., intrinsic herd immunity develops) through the anti-viral effect of tetherin. We also reveal that all Vpus of transmitted/founder HIV-1M viruses maintain anti-tetherin activity. These findings indicate that tetherin plays the role of a host restriction factor, providing 'intrinsic herd immunity', whereas Vpu has evolved in HIV-1M as a tetherin antagonist.
•The formulations for the fluid-particle interaction model are derived based on the volume-averaging.•The fluid force estimation includes the effect of the flow disturbance around the particle.•The ...present model reproduces the interaction between the vortex and the particle of non-negligible size.
To study the dynamics of particles in turbulence when their sizes are comparable to the smallest eddies in the flow, the Kolmogorov length scale, efficient and accurate numerical models for the particle-fluid interaction are still missing. Therefore, we here extend the treatment of the particle feedback on the fluid based on the volume-averaged fluid equations (VA simulation) in the previous study of the present authors, by estimating the fluid force correlated with the disturbed flow. We validate the model against interface-resolved simulations using the immersed-boundary method. Simulations of single particles show that the history effect is well captured by the present estimation method based on the disturbed flow. Similarly, the simulation of the flow around a rotating particle demonstrates that the lift force is also well captured by the proposed method. We also consider the interaction between non-negligible size particles and an array of Taylor–Green vortices. For density ratios ρd /ρc ≥ 10, the results show that the particle motion captured by the VA approach is closer to that of the fully-resolved simulations than that obtained with a traditional two-way coupling simulation. The flow disturbance is also well represented by the VA simulation. In particular, it is found that history effects enhance the curvature of the trajectory in vortices and this enhancement increases with the particle size. Furthermore, the flow field generated by a neighboring particle at distances of around ten particle diameters significantly influences particle trajectories. The computational cost of the VA simulation proposed here is considerably lower than that of the interface-resolved simulation.
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