This study aimed to develop a Japanese version of the motivation to lead (MTL) scale consisting of three factors-affective-identity MTL, non-calculative MTL, and social-normative MTL-and examine its ...construct validity and reliability.
The participants comprised 500 university students and 500 employees aged 20-29 years registered with a Japanese research company.
Based on a confirmatory factor analysis, the three-factor model was found to be appropriate for the Japanese context. The measurement invariance analyses indicated scalar invariance between students and employees and between men and women. Finally, the correlation analysis with the Big Five personality traits conducted to examine construct validity indicated that affective-identity MTL and social-normative MTL had significant relationships with all five traits (extraversion, agreeableness, conscientiousness, open-mindedness, and negative emotionality). Although non-calculative MTL was not significantly correlated, it can be interpreted in the Japanese context.
The results indicate the adequate construct validity and reliability of the Japanese version of the MTL scale. These findings hold significant implications for leadership development and selection in Japan, highlighting the motivational factors that drive effective leadership.
Hepatocellular carcinoma (HCC) is characterized by high intertumor heterogeneity of genetic drivers. Two multitarget tyrosine kinase inhibitors (TKI), lenvatinib and sorafenib, are used as ...standard-of-care chemotherapeutics in patients with advanced HCC, but a stratification strategy has not been established because of a lack of efficacious biomarkers. Therefore, we sought biomarkers that indicate lenvatinib-susceptible HCC.
We performed genetic screening of HCC driver genes involved in TKI susceptibility using a novel HCC mouse model in which tumor diversity of genetic drivers was recapitulated. A biomarker candidate was evaluated in human HCC cell lines. Secreted proteins from HCC cells were then screened using mass spectrometry. Serum and tumor levels of the biomarker candidates were analyzed for their association and prediction of overall survival in patients with HCC.
We found that lenvatinib selectively eliminated FGF19-expressing tumors, whereas sorafenib eliminated MET- and NRAS-expressing tumors. FGF19 levels and lenvatinib susceptibility were correlated in HCC cell lines, and FGF19 inhibition eliminated lenvatinib susceptibility. Lenvatinib-resistant HCC cell lines, generated by long-term exposure to lenvatinib, showed FGF19 downregulation but were resensitized to lenvatinib by FGF19 reexpression. Thus, FGF19 is a tumor biomarker of lenvatinib-susceptible HCC. Proteome and secretome analyses identified ST6GAL1 as a tumor-derived secreted protein positively regulated by FGF19 in HCC cells. Serum ST6GAL1 levels were positively correlated with tumor FGF19 expression in patients with surgically resected HCC. Among patients with serum ST6GAL1-high HCC who underwent TKI therapy, lenvatinib therapy showed significantly better survival than sorafenib.
Serum ST6GAL may be a novel biomarker that identifies lenvatinib-susceptible FGF19-driven HCC.
Mouse trophoblast stem cells (TSCs) have been established and maintained using hyperglycemic conditions (11 mM glucose) for no apparent good reason. Because glucose metabolites are used as resources ...for cellular energy production, biosynthesis, and epigenetic modifications, differences in extracellular glucose levels may widely affect cellular function. Since the hyperglycemic culture conditions used for TSC culture have not been fully validated, the effect of extracellular glucose levels on the properties of TSCs remains unclear. To address this issue, we investigated the gene expression of stemness-related transcription factors in TSCs cultured in the undifferentiated state under various glucose concentrations. We also examined the expression of trophoblast subtype markers during differentiation, after returning the glucose concentration to the conventional culture concentration (11 mM). As a result, it appeared that the extracellular glucose conditions in the stem state not only affected the gene expression of stemness-related transcription factors before differentiation but also affected the expression of marker genes after differentiation, with some line-to-line variation. In the TS4 cell line, which showed the largest glucose concentration-dependent fluctuations in gene expression among all the lines examined, low glucose (1 mM glucose, LG) augmented H3K27me3 levels. An Ezh2 inhibitor prevented these LG-induced changes in gene expression, suggesting the possible involvement of H3K27me3 in the changes in gene expression seen in LG. These results collectively indicate that the response of the TSCs to the change in the extracellular glucose concentration is cell line-dependent and a part of which may be epigenetically memorized.
Abstract
The concept of radiation-induced aging is revisited from the viewpoint of a mathematical model. The effect of radiation on carcinogenesis is treated based on the Armitage–Doll multi-stage ...theory. The formula obtained for cancer incidence rate indicates that radiation dose can be explained in terms of time. Radiation-induced aging for acute and chronic exposures is described using age-specific cancer incidence rates as a measure of aging. It shows that accelerated aging is related to the dose rate, whereas premature aging is related to the cumulative dose, providing a simple and natural interpretation of radiation-induced aging. The usefulness of this approach is demonstrated by applying the formula to cancer prevalence data from mice chronically exposed to low dose-rate radiation.
Ropivacaine-induced myotoxicity in surgically incised muscles has not been fully investigated. We evaluated the effects of infiltration anesthesia with ropivacaine on damage, inflammation and ...regeneration in the incised muscles of rats undergoing laparotomy. Ropivacaine or saline was infiltrated below the muscle fascia over the incised muscles. Pain-related behaviors and histological muscle damage were assessed. Macrophage infiltration at days 2 and 5 and proliferation of satellite cells at day 5 were detected by CD68 and MyoD immunostaining, respectively. Pain-related behaviors were inhibited by 0.25% and 0.5% of ropivacaine for 2 h after surgery. Single infiltration of 0.5% ropivacaine did not induce injury in intact muscles without incision, but single and repeated infiltration of 0.5% ropivacaine significantly augmented laparotomy-induced muscle injury and increased the numbers of CD68-positve macrophages and MyoD-positive cells compared to those in rats with infiltration of saline or 0.25% ropivacaine. In contrast, there were no significant differences in them between rats with saline infusion and rats with 0.25% ropivacaine infiltration. In conclusion, single or repeated subfascial infiltration of 0.25% ropivacaine can be used without exacerbating the damage and inflammation in surgically incised muscles, but the use of 0.5% ropivacaine may be a concern because of potentially increased muscle damage.
Abstract
The mechanisms underlying geocentric (orientations of an object or the body relative to ‘gravity’) and egocentric estimates (object orientation relative to the ‘body’) have each been ...examined; however, little is known regarding the association between these estimates, especially when the body is nearly upright. To address this, we conducted two psychophysical experiments. In Experiment 1, participants estimated the direction of a visual line (subjective visual vertical; SVV) and their own body relative to gravity (subjective body tilt; SBT) and the direction of a visual line relative to the body longitudinal axis (subjective visual body axis; SVBA) during a small-range whole-body roll tilt. We evaluated the correlations between performance on each of these tasks as covariates of actual body tilt angles. Our results showed a significant correlation of performance (estimation errors) on the SVBA task with performance on the SBT task but not performance on the SVV task at the group level after adjusting for the actual body tilt angles, suggesting a link between the estimates for SVBA and SBT tasks. To confirm this relationship, in Experiment 2, we further assessed whether manipulating the subjective direction of the body axis by providing visual feedback in the SVBA task subsequently affected SBT performance. We found that feedback in the SVBA task significantly shifted the SBT angles even when the actual body angles were identical. The observed association between SVBA and SBT performance supports at least a partially shared mechanism underlying body tilt and egocentric estimates when the body is nearly upright.
The thermal conductivity of planetary soils, or regolith, is essential for understanding the present global thermal state of bodies. The thermal conductivity of lunar soils is important with respect ...to lunar crustal heat flow. Although
in situ
measurements were performed by the Apollo 15 and 17 missions, laboratory measurements of the returned lunar samples have not reproduced the estimated subsurface values. Since the amount of extraterrestrial soil samples is limited, a small apparatus is needed to measure their thermal conductivity. In this study, we developed an apparatus enabling the measurement of the thermal conductivity of a small amount of soil (< 10 g)
via
the line heat source method as a function of compressional pressure under vacuum conditions. The thermal conductivity of glass beads and lunar regolith simulant derived by the new apparatus is higher than that obtained from the larger line heat source, and then, reliable apparatus. To evaluate the experimental results, we performed numerical simulation of the temperature evolution during the line heat source measurement, and found that the thermal conductivity derived from the simulation data is higher than the input thermal conductivity. This is consistent with the experimental results and is caused by the heat loss through a line heat source with a limited length. The difference depends on the contact conductance between the sample and the line heat source, and the calibration factors for each sample are determined.
Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of ...the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.
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