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•The sulfides of the ultramafic rocks from the Huangshannan deposit are characterized by high Ni tenor.•High Ni tenor is the result of olivine-sulfide-silicate liquid equilibrium at ...relatively high R values.•They were formed by mechanical transport and deposition of sulfide liquid in a magma pathway or conduit.•Two stage contamination model is indicated by the Os-S isotopes.•Pt and Pd depletion are attributed to sulfide liquid fractionation coupled with Cu-rich sulfide liquid percolation.
The Huangshannan magmatic Ni-Cu sulfide deposit is one of a group of Permian magmatic Ni-Cu deposits located in the southern Central Asian Orogenic belt in the Eastern Tianshan, northwest China. It is characterized by elevated Ni tenor (concentrations in recalculated 100% sulfide) in sulfide within ultramafic rocks (9–19wt%), with values much higher than other deposits in the region. Sulfides of the Huangshannan deposit are composed of pentlandite, chalcopyrite, and pyrrhotite and the host rock is relatively fresh, indicating that the high-Ni tenor is a primary magmatic feature rather than formed by alteration processes. It is shown that sulfides with high-Ni tenor can be generated by sulfide-olivine equilibrium at an oxygen fugacity of QFM +0.5, for magmas containing 450ppm Ni and 20% olivine. Ores with >10wt% sulfur have relatively low PGE and Ni tenors compared to other ores, R factor (mass ratio of silicate to sulfide liquid) modeling of Ni indicates that they formed at moderate R values (150–600). Based on this constraint on R values, ores with <10wt% sulfides in the Huangshannan deposit can be segregated from a similar parental magma with 0.05ppb Os, 0.023ppb Ir, and 0.5ppb Pd at R values between 600 and 3000. This, coupled with the supra-cotectic proportions of sulfide liquid to cumulus silicates in the Huangshannan ores imply mechanical transport and deposition of sulfide liquid in a magma pathway or conduit, in which sulfides must have interacted with large volumes of silicate magma. Platinum and Pd depletion relative to other platinum group elements (PGEs) are observed in fresh and sulfide-rich samples (S>4.5wt%). As sulfide-rich samples are also depleted in Cu, and as interstitial sulfides in those samples are physically interconnected at a scale of several cms, the low Pt and Pd anomalies are attributed to solid Pt and Pd phases crystallization and retention with the monosulfide solid solution (MSS) and Cu-rich sulfide liquid percolation during MSS fractionation. This finding indicates that Pt anomalies in sulfide-rich rocks from magmatic Ni-Cu deposits in the Eastern Tianshan are the result of sulfide fractionation rather than a hydrothermal effect. 187Os/188Os(278Ma) values of the lherzolite samples vary from 0.27 to 0.37 and γOs(278Ma) values vary from 110 to 189, indicating significant magma interaction with crustal sulfides, rich in radiogenic Os. Well constrained γOs values and δ34S values (−0.4 to 0.8‰) indicate that crustal contamination occurred at depth before the arrival of the magma in the Huangshannan chamber. Regionally, deposits with high-Ni tenor have not been reported other than the Huangshannan deposit; however, many intrusions with high-Ni contents in olivine are present in NW China, such as the Erhongwa, Poyi and Poshi intrusions. Those intrusions are capable of forming high-Ni tenor sulfides due to olivine-sulfide-silicate equilibrium and relative high-Ni content in parent magma, making them attractive exploration targets.
Erythromycin fermentation residue (EFR) is a solid waste generated from the fermentation process of erythromycin A production. Some byproducts are produced during the fermentation process of ...erythromycin A production, and erythromycin A can also undergo hydrolysis and biodegradation reactions in the environment with the formation of transformation products. Herein, an accurate analytical method was established and validated to quantify erythromycin A, two byproducts and five hydrolysis or biodegradation products, in solid or semi-solid media of waste EFR and the amended soil. The method mainly included ultrasonic solvent extraction, solid phase extraction, and ultra-performance liquid chromatography-tandem mass spectrometry quantification. All analytes could be effectively extracted in a single process, and the recoveries ranged from 76% to 122% for different matrices. Low matrix effects and excellent precision were achieved by optimizing the mass spectrometry parameters, extraction solution, number of extractions and eluent. This method was applied to evaluate the residual analytes in EFR, treated EFR after industrial-scale hydrothermal treatment, and the subsequent soil application. Seven analytes were detected in the EFR, while six were found in the treated EFR and amended soils. The concentration of erythromycin A in EFR was 1,629 ± 100 mg/kg·TS, and the removal efficiency of hydrothermal treatment (180 °C, 60 min) was about 99.6%. Three hydrolysis products were the main residuals in treated EFR, with anhydroerythromycin A showing the highest concentration. The concentrations of the analytes in soil ranged from 2.17 ± 1.04 to 92.33 ± 20.70 μg/kg·TS, and anhydroerythromycin A contributed 65%–77% of the total concentration. Erythromycin B, a byproduct, was still detected in soil. This work provides an accurate analytical method which would be useful to evaluate the potential risk of byproducts and transformation products of erythromycin A in environment.
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•Erythromycin A and seven transformation products were quantified by UPLC-MS/MS.•Solid phase extraction was optimized to extract analytes from complex matrices.•Seven analytes were detected in erythromycin fermentation residue (EFR), while six were in treated EFR and amended soil.•Three hydrolysis products of ERY-A were the main transformation products in EFR and amended soil.
Two new benzimidazole salts, namely, H2IBI2+ 2X (X = NO3− (1), ClO4− (2) IBI = 2-((1H-imidazol-1-yl)methyl)-1H-benzimidazole, were grown through reacting IBI and two different inorganic acids by slow ...evaporation method, respectively. Compounds 1 and 2 have been characterized by single–crystal X–ray diffraction, IR, UV–Vis, and thermogravimetric analyses (TGA). In both compounds, a set of hydrogen bonds (C/NH⋯O) can be clearly observed, through which a three-dimensional framework will be generated. The luminescent spectra show the emission peaks in compounds 1 and 2 are found at 375 and 371 nm, respectively. By comparison with the free IBI, the emission maxima of compounds 1 and 2 are obviously red-shifted about 67 and 63 nm, respectively.
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•Two salts containing the benzimidazole have been obtained.•C/NH⋯O hydrogen bonds were observed.•Two salts with double protonated moiety were found.•They show the photoluminescent properties.•The red-shifted phenomena were observed.
The purpose of this study was to separate lumbrokinase in Pheretima praepinguis and examine its enzymatic properties. With P. praepinguis as material, lumbrokinase was separated with the salting out ...method. Lumbrokinase activity was measured with casein medium plate method. Effect of pH and temperature on lumbrokinase activity was studied. Results of lumbrokinase separated from P. praepinguis was relatively high. Lumbrokinase activity in neutral or slightly alkaline environment was higher. Lumbrokinase had tolerance ability to high temperature, with highly enzymatic activity under 60°C and wide range of temperature adaptation.
Since the onset of the COVID-19 pandemic, burnout symptoms have been prevalent among healthcare workers. Living with spouses can be complex and was associated with an increased burnout risk during ...the COVID-19 pandemic. This study investigated the relationship between living with spouses and burnout among healthcare workers during the COVID-19 pandemic. We distributed questionnaires to participants working in a hospital affiliated with a medical university in Taiwan. The questionnaires were the Copenhagen Burnout Inventory, which comprises personal burnout (PB), work-related burnout (WB), and client burnout subscales; the Nordic Musculoskeletal Questionnaire; and information on basic demographic variables, family factors, living habits, work-related factors, and physical health factors. Multiple linear regression and mediation analysis were used. We obtained 1615 (63.81%) valid questionnaires. After analysis revealed that marriage was an independent risk factor for PB; however, the effect of marriage on WB was nonsignificant after controlling for risk factors. Parenthood, less alcohol use, reported sleep duration less than six hours, less overtime, less shift work, and participation in leisure activities with family and friends were found to be mediators between marriage and a lower WB level. In addition, chronic diseases, frequent neck pain, and shoulder pain were suppression factors. In summary, marriage was associated with an increased risk of PB. Married individuals sustain a high WB level because of changes in family roles, living conditions, and work conditions. Overall, helping healthcare workers to maintain well-being in marriage or family living may be effective in decreasing burnout during the COVID-19 pandemic.
As an emerging kind of crystalline material, the metal-organic framework (MOF) has shown great promise in the biomedical domains such as drug storage and delivery. In this study, a new porous MOF, ...Dy2(H2O)3(SDBA)3(DMA)6 (1, H2SDBA = 4,4′-sulfonyldibenzoic acid, DMA = N,N-dimethylacetamide (C4H9NO)), with uncoordinated O donor sites has been fabricated using a bent polycarboxylic acid organic linker under the solvothermal condition. The structure of the obtained crystalline product has been fully determined by the X-ray single-crystal diffraction, TGA, elemental analysis, XRD, and the gas sorption measurement. Due to the suitable window size and polar atom functionalized 1D channels, the activated 1 (1a) compound was used for the anticancer drug 5-fluorouracil (5-Fu, C4H3FN2O2) loading by a simple impregnation method. A moderate drug loading and pH-dependent drug-release behavior could be observed for 1a. Furthermore, as indicated by the MTT assay, this drug/MOF composite shows low toxicity toward the human normal cells and demonstrates obvious anticancer activity against the human osteosarcoma cell line MG63.
Abstract Background SARS-CoV-2 continues to mutate over time, and reports on children infected with Omicron BA.5 are limited. We aimed to analyze the specific symptoms of Omicron-infected children ...and to improve patient care. Methods We selected 315 consecutively hospitalized children with Omicron BA.5 and 16,744 non-Omicron-infected febrile children visiting the fever clinic at our hospital between December 8 and 30, 2022. Specific convulsions and body temperatures were compared between the two cohorts. We analyzed potential associations between convulsions and vaccination, and additionally evaluated the brain damage among severe Omicron-infected children. Results Convulsion rates (97.5% vs. 4.3%, $$P$$ P < 0.001) and frequencies (median: 2.0 vs. 1.6, $$P$$ P < 0.001) significantly differed between Omicron-infected and non-Omicron-infected febrile children. The body temperatures of Omicron-infected children were significantly higher during convulsions than when they were not convulsing and those of non-Omicron-infected febrile children during convulsions (median: 39.5 vs. 38.2 and 38.6 °C, both $$P$$ P < 0.001). In the three Omicron-subgroups, the temperature during convulsions was proportional to the percentage of patients and significantly differed ( $$P$$ P < 0.001), while not in the three non-Omicron-subgroups $$(P$$ ( P = 0.244). The convulsion frequency was lower in the 55 vaccinated children compared to the 260 non-vaccinated children (average: 1.8 vs. 2.1, $$P$$ P < 0.001). The vaccination dose and convulsion frequency in Omicron-infected children were significantly correlated ( $$P$$ P < 0.001). Fifteen of the 112 severe Omicron cases had brain damage. Conclusions Omicron-infected children experience higher body temperatures and frequencies during convulsions than those of non-Omicron-infected febrile children. We additionally found evidence of brain damage caused by infection with omicron BA.5. Vaccination and prompt fever reduction may relieve symptoms. Graphical abstract
Three metal coordination polymers with a one-dimensional motif have been obtained, which are constructed from transition mental salts with the 4PBI ligand and aromatic carboxylic acids under ...hydrothermal reaction conditions. The luminescent properties of complexes 2 and 3 have been investigated.
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Three new metal coordination complexes, namely Co(IPA)(4PBI)·H2O (1), Cd(IPA)(4PBI)(H2O) (2) and Cd(TPA)(4PBI)(H2O) (3) 4PBI=2-(pyridine-4-yl)-(1H)-benzoimidazole, IPA=isophthalate and TPA=terephthalate, were obtained under hydrothermal conditions by reacting cobalt and cadmium salts with 4PBI and IPA or TPA. Complexes 1–3 were structurally characterized by X-ray single-crystal diffraction, EA, IR and PXRD. All of these complexes display low dimensional features with one-dimensional (1D) motifs. Complex 1 is a 1D tape-like structure containing bi-nuclear units, which is further extended into a 3D supramolecular architecture through intermolecular hydrogen bonds and stacking interactions. Complex 2 shows a 1D structural motif, which is further assembled into a 3D supramolecular architecture by hydrogen bonds and packing interactions. Complex 3 is also a 1D chain. Through intermolecular hydrogen bonds and π⋯π packing interactions, a 3D supramolecular network is also generated for 3. The luminescent properties of complexes 2 and 3 were investigated in the solid state at room temperature. Additionally, thermogravimetric analysis (TGA) of 1–3 has been discussed in details.
This study aimed to explore the relationship of D-dimer level with the risk stratification of ischemic stroke, and determine whether high D-dimer levels could be used as a risk factor of ischemic ...stroke in patients with nonvalvular atrial fibrillation (NVAF).This single-center, retrospective study recruited NVAF patients who did not undergo anticoagulant therapy. These patients were divided into 2 groups: ischemic stroke group and no-stroke group. The medical records of each patient were reviewed, demographic and clinical analyses were performed, and the laboratory results were summarized.A total of 323 eligible in-patients with NVAF, who did not receive anticoagulant therapy, were recruited (206 male and 117 female patients, median age was 75.18 ± 10.46 years old). Among these patients, 78 patients suffered from acute ischemic stroke. D-dimer level increased with age, and was positively correlated with the risk stratification of stroke, CHADS2 score (rs = 0.441, P < .001), and CHA2DS2-VASC score (rs = 0.412, P < .001), even after adjustment for age and gender (rs = 0.422, P < .001). The difference in baseline D-dimer level between these 2 groups was not statistically significant (0.70 vs 0.66 mg/L, P = .330), but this significantly increased when patients suffered from stroke (1.34 vs 0.70 mg/L, P < .001). The D-dimer level after stroke (≥6 months) was also higher than the baseline (1.16 vs 0.68 mg/L, P = .514) in 6 months, and this level nearly returned to baseline level after one year (0.69 vs 0.68 mg/L, P = .158). However, logistic regression revealed that only the D-dimer level at stroke onset and OMI were independent risk factors for ischemic stroke (P < .001), while the increase from baseline D-dimer levels was not an independent risk factor (P = .125).D-dimer level is positively correlated with the risk stratification of ischemic stroke, but has no predictive value on the occurrence of ischemic stroke in patients with NVAF.
Background. Little is known about the induction of functional and brain structural reorganization in hemiplegic cerebral palsy (HCP) by constraint-induced movement therapy (CIMT). Objective. We aimed ...to explore the specific molecular mechanism of functional and structural plasticity related to CIMT in HCP. Methods. The mice were divided into a control group and HCP groups with different interventions (unconstraint-induced movement therapy UNCIMT, CIMT or siRNA-Nogo-A SN treatment): the HCP, HCP+UNCIMT, HCP+CIMT, HCP+SN, and HCP+SN+CIMT groups. Rotarod and front-limb suspension tests, immunohistochemistry, Golgi-Cox staining, transmission electron microscopy, and Western blot analyses were applied to measure motor function, neurons and neurofilament density, dendrites/axon areas, myelin integrity, and Nogo-A/NgR/RhoA/ROCK expression in the motor cortex. Results. The mice in the HCP+CIMT group had better motor function, greater neurons and neurofilament density, dendrites/axon areas, myelin integrity, and lower Nogo-A/NgR/RhoA/ROCK expression in the motor cortex than the HCP and HCP+UNCIMT groups (P < .05). Moreover, the expression of Nogo-A/NgR/RhoA/ROCK, the improvement of neural remodeling and motor function of mice in the HCP+SN group were similar to those in the HCP+CIMT group (P > .05). The neural remodeling and motor function of the HCP+SN+CIMT group were significantly greater than those in the HCP+SN and HCP+CIMT groups (P < .05). Motor function were positively correlated with the density of neurons (r = 0.450 and 0.309, respectively; P < .05) and neurofilament (r = 0.717 and 0.567, respectively; P < .05). Conclusions. CIMT might promote the remodeling of neurons, neurofilament, dendrites/axon areas, and myelin in the motor cortex by partially inhibiting the Nogo-A/NgR/RhoA/ROCK pathway, thereby promoting the improvement of motor function in HCP mice.
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