EFFECT OF ADJUVANTS ON LOCAL sIgA RESPONSES Ebersole, Jeffrey L.; Taubman, Martin A.; Smith, Daniel J.
Annals of the New York Academy of Sciences,
June 1983, Letnik:
409, Številka:
1
Journal Article
In two previously well nonsmokers fatal pneumonia developed with extensive abscess formation. Legionnaires' bacillus was the only pathogen isolated. These cases indicate that Legionnaires' bacillus ...is capable of causing extensive necrosis of the lung.
The level and distribution of static crevicular fluid (SCF) antibody was studied in periodontal disease patients. Elevated levels of SCF antibody were demonstrated in approximately 9% of 1292 sites. ...The elevated responses were shown to be limited to particular bacterial specificities at any one site. Additional comparisons indicated that the frequency of elevated antibody levels in SCF were most closely related with responses to A. actinomycetemcomitans, B. gingivalis, and B. intermedius rather than clinical categorization.
A method was developed for the culture and the measurement of immunoglobulin (Ig) synthesis by the relatively small numbers of mononuclear cells recoverable from periodontal disease tissues. In this ...system, we demonstrated that mononuclear cells actively synthesize Ig, remain viable and proliferate. The sensitivity of the quantitative Biotin‐Avidin enzyme linked immunoadsorbent assay (ELISA) procedure developed was tenfold greater than the conventional ELISA for IgG, IgM and IgA measurement. Studies of Ig synthesis by varying cell numbers indicated that 25,000 cells synthesized at least as much IgG, IgA and IgM as any higher number of cells (50‐500,000) tested. Varying the numbers of cells cultured also did not alter the kinetics of Ig synthesis in this system. The composition of the gingival mononuclear cells was studied. These cells consisted of approximately 21% T4 positive cells, 20% T8 positive cells, 42% Ig‐positive cells and 6% adherent, NSE‐positive cells. Ig synthesis by gingival and PB cells in the presence and absence of mitogen was used as an indicator of functional capability of the resident population derived from diseased periodontal tissues.
Summary from only given. Noise-immune cavity-enhanced optical heterodyne molecular spectroscopy (NICE-OHMS) was used to detect the Lamb-dip of nitrous oxide (N/sub 2/O). We used both direct ...cavity-enhanced absorption and NICE-OHMS. We used a quantum cascade laser operating at 8.5 microns which was tightly locked by the Pound-Drever-Hall technique.
Studies concerned with medication compliance after initial prescription dispensing have been frequently cited in the literature; however, little research has been done on the initial filling of the ...prescription order. In order to ascertain the degree of compliance at the initial filling level, a study was carried out on a population associated with a neighborhood health center. Overall, the study indicated an initial filling compliance of 94 percent, which was affected by the site of patient encounter, the chronicity of the disease, and the specificity of the medication in relationship to the disease state. It was concluded that: prescriptions for chronic medications are filled at a higher rate than those for acute illnesses; specific medication for a specific illness has a much higher compliance rate than nondisease specific medications; ease of access to a pharmacy is related to a higher compliance level; and frequent patient contact for a specific disease tends to increase compliance.
A sensitive modification of the ammonium sulfate precipitation assay for antibody affinity is described. The assay combines small reaction volumes and a 32P volume marker to determine the average ...relative affinity of small amounts of antibody to DNP hapten. The assay is relatively safe since two beta-particle emitters are utilized instead of two gamma-ray emitters. The assay has proven useful for the determination of small amounts of antibody such as might be found in secretions and column fractions.
1) Glucosyltransferase serotypes a and g are closely related antigenically but are more distantly related to GTF of serotype c, based on assays of inhibition of total glucan synthesis. 2) Local ...immunization with GTF from serotype c S. mutans reduces the colonization, caries, and lesions caused by infection with the homologous strain compared with sham injected controls. 3) Local immunization with GTF of serotype c S. mutans reduces the colonization, caries, and lesions caused by infection with S. mutans of serotype g (strain 6715).
