We tested three oxidant sensitive fluoroprobes (dihydrorhodamine DHR, 2′,7′-dichlorodihydrofluorescein H2DCF, and dihydroethidium DHE) for interactions with three inhibitors of mitochondrial electron ...transport. DHR, H2DCF, and DHE produced large time-dependent increases in fluorescence in a cell-free medium that contained either of the site III inhibitors antimycin (A) and 2-heptyl-4-hydroxy-quinoline-N-oxide but minimal increases in medium that contained another site III inhibitor, myxothiazol (Mx). The interactions between A and each of the fluoroprobes occurred at concentrations of agent/probe that are frequently used in experiments designed to investigate cellular oxidant production. To define more effectively the nature of these agent/probe interactions, we determined the oxygen dependence of the interactions between A and each probe. The A/H2DCF and A/DHR interactions either were highly oxygen-dependent or exhibited a small degree of oxygen dependence, respectively, whereas the A/DHE interaction was oxygen-independent. Finally, we determined multiple ways to reduce the impact of the agent/probe interaction on data acquisition. The addition of either fetal bovine serum (10%) or albumin (5%) to the media abolished the A/DHR and A/H2DCF interactions. Shifting the excitation wavelength of DHE (from 470 to 530 nm) reduced measurement of the A/DHE interaction while preserving measurement of the intracellular signal. Collectively, these results emphasize the importance of testing for interactions between agents and probes, because these interactions can interfere with the accurate interpretation of experimental results. In addition, the methods presented for circumventing these interactions may be applicable to other experiments in which agent/probe interactions are an obstacle to accurate interpretation of the experimental results.
Abstract
Dwarf spheroidal galaxies (dSphs) are ideal targets for Dark Matter (DM) indirect searches due to their high DM content and their negligible expected astrophysical background. In this ...presentation, we report on the combined analysis of the observations of 20 dSphs by
Fermi
-LAT, HAWC, H.E.S.S., MAGIC, and VERITAS collaborations in the search for DM, focusing on the Weakly Interacting Massive Particles (WIMPs) scenario. The combined analysis allows us to maximize the sensitivity by combining individual data sets from all five experiments for which the energy ranges of the search overlap. New constraints, spanning a range of DM masses from 5 GeV to 100 TeV, on the velocity-weighted cross section for DM self annihilation will be presented.
Primordial Black Holes (PBHs) are black holes that may have been created by density fluctuations in the early Universe and could be as massive as supermassive black holes or as small as the Planck ...scale. It is believed that a black hole has a temperature inversely proportional to its mass and will thermally emit all species of fundamental particles via Hawking Radiation. PBHs with initial masses of ~5e14 g (approximately one gigaton) should be expiring today with bursts of high-energy gamma radiation in the GeV – TeV energy range. The High Altitude Water Cherenkov (HAWC) Observatory is sensitive to particles with energies of 300 GeV – 100 TeV, which corresponds to the high end of the PBH burst spectrum. With its large instantaneous field of view of ~2 sr and a duty cycle above 95%, the HAWC Observatory is well suited to perform an all-sky search for PBH bursts. We conducted a search on 959 days of gamma-ray data from HAWC by optimizing a previous gamma-ray burst transient search to the PBH burst energy spectrum, and placed the strongest upper limits on the local PBH burst rate density at the 99% confidence level.
The search for Dark Matter (DM) has great potential to reveal physics beyond the Standard Model. As such, searches for evidence of DM particles are being carried out using a wide range of techniques, ...such as direct searches for DM particles, searches for DM produced with colliders, and indirect searches for the Standard Model annihilation products of DM. Dwarf spheroidal galaxies (dSphs) are excellent targets for indirect Dark Matter searches due to their relatively high DM content and negligible expected astrophysical background. A collaboration was formed to maximise the sensitivity of DM searches towards dSphs by combining for the first time dSph data from three imaging air Cherenkov telescope (IACT) arrays: HESS, MAGIC, and VERITAS; the Fermi-LAT satellite, and the water Cherenkov detector HAWC. Due to the diverse nature of the instruments involved, each experiment will analyse their individual datasets from multiple targets and then the results will be combined at the likelihood level. For consistency of the likelihoods across the five experiments, a common approach is used to treat the astrophysical factor (J-Factor) for each target and an agreed set of annihilation channels are considered. We also agree on a com- mon statistical approach and treatment of instrumental systematic uncertainties. The results are presented in terms of constraints on the velocity-weighted cross section for DM self-annihilation as a function of the DM particle mass.
▪ Abstract We review the direct measurements of the top quark mass M
t
using the sample of Formula: see text events collected by the DØ and CDF experiments at Fermilab. Measurements using events in ...the lepton plus jets, dilepton, and all-hadronic Formula: see text decay modes are reviewed, as is the combination of the results to yield the current world average M
t
= 174.3 ± 3.2 (stat.) ± 4.0 (syst.) GeV/c
2
. We close by estimating the precision attainable with future data sets at Fermilab and the Large Hadron Collider.
Snowmass Cosmic Frontier Report Chou, Aaron S; Soares-Santos, Marcelle; Tait, Tim M P ...
arXiv (Cornell University),
11/2022
Paper, Journal Article
Odprti dostop
This report summarizes the current status of Cosmic Frontier physics and the broad and exciting future prospects identified for the Cosmic Frontier as part of the 2021 Snowmass Process.
Cosmological and astrophysical observations suggest that 85\% of the total matter of the Universe is made of Dark Matter (DM). However, its nature remains one of the most challenging and fundamental ...open questions of particle physics. Assuming particle DM, this exotic form of matter cannot consist of Standard Model (SM) particles. Many models have been developed to attempt unraveling the nature of DM such as Weakly Interacting Massive Particles (WIMPs), the most favored particle candidates. WIMP annihilations and decay could produce SM particles which in turn hadronize and decay to give SM secondaries such as high energy \(\gamma\) rays. In the framework of indirect DM search, observations of promising targets are used to search for signatures of DM annihilation. Among these, the dwarf spheroidal galaxies (dSphs) are commonly favored owing to their expected high DM content and negligible astrophysical background. In this work, we present the very first combination of 20 dSph observations, performed by the Fermi-LAT, HAWC, H.E.S.S., MAGIC, and VERITAS collaborations in order to maximize the sensitivity of DM searches and improve the current results. We use a joint maximum likelihood approach combining each experiment's individual analysis to derive more constraining upper limits on the WIMP DM self-annihilation cross-section as a function of DM particle mass. We present new DM constraints over the widest mass range ever reported, extending from 5 GeV to 100 TeV thanks to the combination of these five different \(\gamma\)-ray instruments.
PDF
