The STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) statement was first published in 2007 and again in 2014. The purpose of the original STROBE was to provide guidance ...for authors, reviewers, and editors to improve the comprehensiveness of reporting; however, STROBE has a unique focus on observational studies. Although much of the guidance provided by the original STROBE document is directly applicable, it was deemed useful to map those statements to veterinary concepts, provide veterinary examples, and highlight unique aspects of reporting in veterinary observational studies. Here, we present the examples and explanations for the checklist items included in the STROBE‐Vet statement. Thus, this is a companion document to the STROBE‐Vet statement methods and process document (JVIM_14575 “Methods and Processes of Developing the Strengthening the Reporting of Observational Studies in Epidemiology—Veterinary (STROBE‐Vet) Statement” undergoing proofing), which describes the checklist and how it was developed.
Background
Reporting of observational studies in veterinary research presents challenges that often are not addressed in published reporting guidelines.
Objective
To develop an extension of the ...STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) statement that addresses unique reporting requirements for observational studies in veterinary medicine related to health, production, welfare, and food safety.
Design
Consensus meeting of experts.
Setting
Mississauga, Canada.
Participants
Seventeen experts from North America, Europe, and Australia.
Methods
Experts completed a pre‐meeting survey about whether items in the STROBE statement should be modified or added to address unique issues related to observational studies in animal species with health, production, welfare, or food safety outcomes. During the meeting, each STROBE item was discussed to determine whether or not rewording was recommended and whether additions were warranted. Anonymous voting was used to determine consensus.
Results
Six items required no modifications or additions. Modifications or additions were made to the STROBE items 1 (title and ), 3 (objectives), 5 (setting), 6 (participants), 7 (variables), 8 (data sources/measurement), 9 (bias), 10 (study size), 12 (statistical methods), 13 (participants), 14 (descriptive data), 15 (outcome data), 16 (main results), 17 (other analyses), 19 (limitations), and 22 (funding).
Conclusion
The methods and processes used were similar to those used for other extensions of the STROBE statement. The use of this STROBE statement extension should improve reporting of observational studies in veterinary research by recognizing unique features of observational studies involving food‐producing and companion animals, products of animal origin, aquaculture, and wildlife.
Nutrient Sensing in Cancer Torrence, Margaret E; Manning, Brendan D
Annual review of cancer biology,
03/2018, Letnik:
2, Številka:
1
Journal Article
Recenzirano
Cell-intrinsic mechanisms of nutrient sensing are intimately linked to adaptive metabolic responses, and these pathways play critical roles in the complex and dynamic nutrient environment of a ...growing tumor. Nutrient-responsive transcription factors (e.g., HIF, SREBP, ATF4) and signaling pathways (e.g., mTORC1, AMPK) allow tumor cells to tune their metabolic output and strategies to fluctuations in nutrient availability, thus balancing tumor cell proliferation and survival with a combination of anabolic and adaptive responses. Coupling these nutrient-sensing mechanisms to the control of recycling and scavenging processes, such as autophagy and macropinocytosis, further enhances the adaptability to nutrients within tumors. Here, we discuss the key nutrient-sensing pathways active in cancer cells, how oncogenic events influence these pathways, and their likely contributions to tumor growth and survival. A better understanding of nutrient-sensing strategies and metabolic adaptations within the tumor microenvironment is critical to defining and targeting metabolic vulnerabilities in cancer.
Epoxy adhesives are experiencing widespread use in concrete structures. However, a common concern regarding the adhesive joints in the infrastructure is their durability when exposed to harsh ...environments, most particularly, high levels of moisture. This work recognizes that adhesive bond between epoxy and substrate resists applied loads by a combination of chemical (hydrogen) bonds and mechanical interlock. Given the complexity of the stress-transfer mechanism this work focused exclusively on the chemical bond component between epoxy and cement paste, while the mechanical interlock was minimized through polishing of the cement paste substrate. A beam adhesion test method with notched interface was developed to assess the durability of chemical bonds between the adherents when aged by water immersion; surface functionalization of cement paste substrate was additionally explored as means of improving the chemical bonding and adhesion along the interface. Test results indicated that interfacial fracture energies were improved in both dry and conditioned groups with silane surface treatment. Analysis of interfacial failure modes with respect to the analytical crack kink criterion revealed that interphase region between epoxy and cement paste is characterized with higher fracture toughness than the cement paste substrate. The study lays groundwork for improvement in the durability of adhesive joints in related infrastructure through bottom-up interface design.
Employee engagement research is typified by the relabeling and reinvention of classic job attitude concepts. In this article, the authors comment on the development of the Work Cognition Inventory ...(WCI), an instrument designed to assess eight major antecedents of employee engagement/work passion. The antecedents measured by the WCI include job autonomy, feedback, task significance, distributive justice, leader-member exchange (LMX), social support, coworker collaboration, and opportunities for growth. The authors note the WCI was created with the use of many of the best scale-development procedures available (e.g., confirmatory factor analyses across several replication samples, convergent and discriminant validity evidence), and is exemplary in this regard. Nonetheless, the WCI appears to measure the same constructs as several long-established instruments. The natural question is whether this new WCI measure of classic human resources (HR) concepts (job autonomy, justice, etc.) is distinct from and performs better than the classic measures of these same concepts. This is an empirical question, but one the authors of the WCI do not address. When attempting to supplant classic measures of well-known constructs, it is important to include those classic measures in the validity study. (Contains 1 figure and 1 table.)
Cyclospora cayetanensis is a parasite causing cyclosporiasis (an illness in humans). Produce (fruits, vegetables, herbs), water and soil contaminated with C. cayetanensis have been implicated in ...human infection. The objective was to conduct a scoping review of primary research in English on the detection, epidemiology and control of C. cayetanensis with an emphasis on produce, water and soil. MEDLINE® (Web of ScienceTM), Agricola (ProQuest), CABI Global Health, and Food Science and Technology Abstracts (EBSCOhost) were searched from 1979 to February 2020. Of the 349 relevant primary research studies identified, there were 75 detection-method studies, 40 molecular characterisation studies, 38 studies of Cyclospora in the environment (33 prevalence studies, 10 studies of factors associated with environmental contamination), 246 human infection studies (212 prevalence/incidence studies, 32 outbreak studies, 60 studies of environmental factors associated with non-outbreak human infection) and eight control studies. There appears to be sufficient literature for a systematic review of prevalence and factors associated with human infection with C. cayetanensis. There is a dearth of publicly available detection-method studies in soil (n = 0) and water (n = 2), prevalence studies on soil (n = 1) and studies of the control of Cyclospora (particularly on produce prior to retail (n = 0)).
This introductory article provides an overview of preharvest food safety activities and initiatives for the past 15 years. The section on traditional areas of preharvest food safety focuses on ...significant scientific advancements that are a culmination of collaborative efforts (both public health and agriculture) and significant research results. The highlighted advancements provide the foundation for exploring future preharvest areas and for improving and focusing on more specific intervention/control/prevention strategies. Examples include Escherichia coli and cattle, Salmonella and Campylobacter in poultry, and interventions and prevention and control programs. The section on "nontraditional" preharvest food safety areas brings attention to potential emerging food safety issues and to future food safety research directions. These include organic production, the FDA's Produce Rule (water and manure), genomic sequencing, antimicrobial resistance, and performance metrics. The concluding section emphasizes important themes such as strategic planning, coordination, epidemiology, and the need for understanding food safety production as a continuum. Food safety research, whether at the pre- or postharvest level, will continue to be a fascinating complex web of foodborne pathogens, risk factors, and scientific and policy interactions. Food safety priorities and research must continue to evolve with emerging global issues, emerging technologies, and methods but remain grounded in a multidisciplinary, collaborative, and systematic approach.
This article documents the muon reconstruction and identification efficiency obtained by the ATLAS experiment for 139
fb
-
1
of
pp
collision data at
s
=
13
TeV collected between 2015 and 2018 during ...Run 2 of the LHC. The increased instantaneous luminosity delivered by the LHC over this period required a reoptimisation of the criteria for the identification of prompt muons. Improved and newly developed algorithms were deployed to preserve high muon identification efficiency with a low misidentification rate and good momentum resolution. The availability of large samples of
Z
→
μ
μ
and
J
/
ψ
→
μ
μ
decays, and the minimisation of systematic uncertainties, allows the efficiencies of criteria for muon identification, primary vertex association, and isolation to be measured with an accuracy at the per-mille level in the bulk of the phase space, and up to the percent level in complex kinematic configurations. Excellent performance is achieved over a range of transverse momenta from 3 GeV to several hundred GeV, and across the full muon detector acceptance of
|
η
|
<
2.7
.
A search for heavy neutral Higgs bosons is performed using the LHC Run 2 data, corresponding to an integrated luminosity of 139 fb−1 of proton-proton collisions at √s = 13 TeV recorded with the ...ATLAS detector. The search for heavy resonances is performed over the mass range 0.2–2.5 TeV for the τ+ τ− decay with at least one τ -lepton decaying into final states with hadrons. The data are in good agreement with the background prediction of the standard model. In the Mh125 scenario of the minimal supersymmetric standard model, values of tan β > 8 and tan β > 21 are excluded at the 95% confidence level for neutral Higgs boson masses of 1.0 and 1.5 TeV, respectively, where tan β is the ratio of the vacuum expectation values of the two Higgs doublets.
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