The relationship between species diversity and spatial scale is a central topic in spatial community ecology. Latitudinal gradient is among the core mechanisms driving biodiversity distribution on ...most scales. Patterns of
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-diversity along latitudinal gradient have been well studied for aboveground terrestrial and marine communities, whereas soil organisms remain poorly investigated in this regard. The West Siberian Plain is a good model to address diversity scale-dependence since the latitudinal gradient does not overlap with other possible factors such as elevational or maritime. Here, we collected 111 samples following hierarchical sampling (sub-zones, ecosystem types, microhabitat and replicate samples) and performed multi-scale partitioning of
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-diversity of testate amoeba assemblages as a model of study. We found that among-ecosystem
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-diversity is a leading scale in testate amoeba assemblages variation. Rare species determine
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-diversity at all scale levels especially in the northern regions, where rare taxa almost exclusively accounted for the diversity at the ecosystem level.
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-Diversity is generally dominated by the turnover component at all scales in lower latitudes, whereas nestedness prevailed at among-ecosystem scale in higher latitudes. These findings indicate that microbial assemblages in northern latitudes are spatially homogeneous and constrained by historical drivers at larger scales, whereas in southern regions, it is dominated by the turnover component both at the microhabitat and ecosystem scales and therefore determined by recent vegetation and environmental heterogeneity. Overall, we have provided the evidence for the existence of negative latitudinal gradient for among-ecosystem
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-diversity but not for among-microhabitat and among-sample
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-diversity for terrestrial testate amoeba communities.
Understanding the interactions among the functional groups of living organisms within ecosystems is a main challenge in ecology. This question is particularly important in relation to the ...interactions between the above- and below-ground components of terrestrial ecosystems. We investigated the effects of macro- (geographic position and mire size) and micro-environmental (pH, water table depth, water mineralization and temperature) characteristics and vegetation composition (both vascular plants and bryophytes) on the species structure of testate amoeba assemblages in eight Sphagnum-dominated mires across the Northern Caucasus Mountains (Russia). In total, 97 testate amoeba species from 34 genera were identified. A multiple factor analysis indicated the strongest relationships between the species structure of the testate amoeba assemblages and the local vegetation, especially bryophytes, whereas the interaction with the micro-environmental characteristics was the weakest. Among the micro-environmental data, the strongest effects on the species composition of all the assemblages were detected for the pH followed by the water table depth and water temperature. The variance partitioning of the species structure of the testate amoeba assemblages in response to the abiotic and biotic data indicated that most of the variance was related to the bryophyte and vascular plant assemblages, whereas the contribution of the environmental data was lower. Moreover, most of the effects were highly related to each other, so that the proportion of the jointly explained variation was high, whereas the individual effects were much lower.
Samples for analysis of testate amoebae and other protists frequently need to be stored for many months before microscopy. This storage commonly involves refrigeration, but we know that testate ...amoebae can live and reproduce in these conditions. This raises the question: do communities change during storage and how might this effect the data produced? We analysed Sphagnum samples over a 16-week period to address this question. Our results show no evidence for detectable change. This is a reassuring result supporting much current practice although we suggest that frozen storage or the addition of a fixative may be worthwhile precautions where feasible.
Testate amoebae are widely used as proxies in paleoecological reconstructions of lacustrine environments; however, our knowledge on their distribution along depth gradients are limited. This study ...investigates the distribution of benthic testate amoebae along a sampling depth gradient (0 to 57 m) and related environmental characteristics in Lake Valdayskoe, Russia. In total, 101 species belonging to twenty-one genera were identified. Four types of testate amoeba assemblages (littoral, sublittoral, bottom slope and profundal) were distinguished that corresponded well to the bottom zones of the lake. The results of redundancy analysis indicated that sampling depth, temperature, pH and bottom inclination significantly explained 40.2% of the total variance in the species composition. Temperature and sampling depth had the largest individual contributions of 19.2 and 7.4% (p < 0.001), respectively. The minimal values of species diversity were observed on the littoral and at the lower boundary of the thermocline. We estimated depth optima and ranges for the species with high occurrences and distinguish stenobathic and eurybathic species. These data might improve the interpretations of paleoecological records of subfossil testate amoeba assemblages in lacustrine surface sediments and serve as basis for the development of a transfer function for reconstruction of lake depths.
Global climate change might result in permafrost thaw and the formation of thermokarst landscapes that release long-term carbon stocks as greenhouse into the atmosphere, thereby initiating a positive ...climate feedback. These processes are mediated by biological activity, including by microbes, vascular plants and animals, whereas the role of invertebrates in thermokarst ecosystems remains poorly understood. We investigated the diversity and assemblage structures of zooplankton (mainly Copepoda, Cladocera), microbenthos (testate amoebae) and meio- (Copepoda and Cladocera) and macrozoobenthos (mollusks, crustaceans, insects and annelids) from a range of water bodies representing different stages of thermokarst lake formation in the southern part of the Lena River Delta (Central Siberia). Altogether, 206 species of testate amoeba, mollusk, crustacean, insect and annelid taxa were identified. A total of 60 species of macrozoobenthos (mainly insects) and 62 species of testate amoebae were detected in the water bodies of the Lena River Delta for the first time. The species richness of zooplankton and meio- and macrozoobenthos was greater in the large thermokarst lakes than in the polygonal ponds due to the freezing of the latter in the winter. In contrast, the species richness of protists was higher in the polygonal ponds, which was related to the habitat preferences of testate amoebae. Fish grazing strongly affected the macrobenthos assemblages but not the smaller-sized organisms. Water acidity and temperature were the main environmental drivers of the assemblage structure of testate amoeba and microcrustacean. The species structure of the macroinvertebrate assemblages was significantly explained by water acidity, permafrost depth and size of the water area. It means that small size organisms with their short generation times are sensitive to more dynamic factors such as temperature and may serve as indicators of ecosystem changes due to global climate warming. In contrast, large size organisms are affected by driven factors that appear during thermokarst lakes formation and permafrost degradation.
Global climate warming disproportionately affects high-latitude and mountainous terrestrial ecosystems. Warming is accompanied by permafrost thaw, shorter winters, earlier snowmelt, more intense soil ...freeze-thaw cycles, drier summers, and longer fire seasons. These environmental changes in turn impact surface water and groundwater flow regimes, water quality, greenhouse gas emissions, soil stability, vegetation cover, and soil (micro)biological communities. Warming also facilitates agricultural expansion, urban growth, and natural resource development, adding growing anthropogenic pressures to cold regions' landscapes, soil health, and biodiversity. Further advances in the predictive understanding of how cold regions' critical zone processes, functions, and ecosystem services will continue to respond to climate warming and land use changes require multiscale monitoring technologies coupled with integrated observational and modeling tools. We highlight some of the major challenges, knowledge gaps, and opportunities in cold region critical zone research, with an emphasis on subsurface processes and responses in both natural and agricultural ecosystems.
For the first time, novel method for the preparation of previously undescribed substituted 2-arylfuro3,2-bchromen-3-ylacetic acids was developed. The considered approach includes multicomponent ...reaction of various arylglyoxals, 3-hydroxy-4H-chromen-4-ones, and Meldrum’s acid. The studied condensation is a telescoped process consisting of primary base-catalyzed interaction of starting compounds in acetonitrile and conclusive cyclization to target furo3,2-bchromen-3-ylacetic acids under acidic conditions. The readily available starting materials, convenient experimental procedure and simple isolation of the final products are advantages of presented method.
Paleoecological reconstructions of hydrological regimes in tropical peatlands during the Holocene are important for the estimation of their responses to changing environments. However, the ...application of some widely used proxies, such as testate amoebae, is hampered by poor knowledge of their morphology and ecological preferences in the region. The aim of this study is to describe the morphospecies composition of sub-fossil testate amoebae in deposits of a tropical peatland in Central Sumatra (Indonesia) during the Holocene and reconstruct the hydrological regime using morphospecies- and functional-trait-based approaches. In total, 48 testate amoeba morphospecies were observed. Based on morphospecies composition, we distinguished three main periods of peatland development (13,400–8000, 8000–2000, 2000 cal yr BP–present). The application of the morphospecies-based transfer function provided a more reliable reconstruction of the water regime in comparison to the functional trait-based one. The weak performance of the latter might be related to the poor preservation of shells and the greater variation in the functional traits in sub-fossil communities as compared to the training set and linear modeling approach. These results call for future studies on the functional and morphospecies composition of testate amoebae in a wider range of tropical peatlands to improve the quality of hydrological reconstructions.
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