Fundamental insights into the macromolecular architecture of plant cell walls will elucidate new structure–property relationships and facilitate optimization of catalytic processes that produce fuels ...and chemicals from biomass. Here we introduce computational methodology to extract nanoscale geometry of cellulose microfibrils within thermochemically treated biomass directly from electron tomographic data sets. We quantitatively compare the cell wall nanostructure in corn stover following two leading pretreatment strategies: dilute acid with iron sulfate co-catalyst and ammonia fiber expansion (AFEX). Computational analysis of the tomographic data is used to extract mathematical descriptions for longitudinal axes of cellulose microfibrils from which we calculate their nanoscale curvature. These nanostructural measurements are used to inform the construction of atomistic models that exhibit features of cellulose within real, process-relevant biomass. By computational evaluation of these atomic models, we propose relationships between the crystal structure of cellulose Iβ and the nanoscale geometry of cellulose microfibrils.
We perform a comparison of different approaches to star-galaxy classification using the broad-band photometric data from Year 1 of the Dark Energy Survey.
Vitamin D deficiency has potential adverse effects on neurocognitive health and subcortical function. However, no studies have examined the association between vitamin D status, dementia, and cranial ...MRI indicators of cerebrovascular disease (CVD).
Cross-sectional investigation of 25-hydroxyvitamin D 25(OH)D, dementia, and MRI measures of CVD in elders receiving home care (aged 65-99 years) from 2003 to 2007.
Among 318 participants, the mean age was 73.5 +/- 8.1 years, 231 (72.6%) were women, and 109 (34.3%) were black. 25(OH)D concentrations were deficient (<10 ng/mL) in 14.5% and insufficient (10-20 ng/mL) in 44.3% of participants. There were 76 participants (23.9%) with dementia, 41 of which were classified as probable AD. Mean 25(OH)D concentrations were lower in subjects with dementia (16.8 vs 20.0 ng/mL, p < 0.01). There was a higher prevalence of dementia among participants with 25(OH)D insufficiency (< or =20 ng/mL) (30.5% vs 14.5%, p < 0.01). 25(OH)D deficiency was associated with increased white matter hyperintensity volume (4.9 vs 2.9 mL, p < 0.01), grade (3.0 vs 2.2, p = 0.04), and prevalence of large vessel infarcts (10.1% vs 6.9%, p < 0.01). After adjustment for age, race, sex, body mass index, and education, 25(OH)D insufficiency (< or =20 ng/mL) was associated with more than twice the odds of all-cause dementia (odds ratio OR = 2.3, 95% confidence interval CI 1.2-4.2), Alzheimer disease (OR = 2.5, 95% CI 1.1-6.1), and stroke (with and without dementia symptoms) (OR = 2.0, 95% CI 1.0-4.0).
Vitamin D insufficiency and deficiency was associated with all-cause dementia, Alzheimer disease, stroke (with and without dementia symptoms), and MRI indicators of cerebrovascular disease. These findings suggest a potential vasculoprotective role of vitamin D.
The terrestrial carbon sink, as of yet unidentified, represents 15-30% of annual global emissions of carbon from fossil fuels and industrial activities. Some of the missing carbon is sequestered in ...vegetation biomass and, under the Kyoto Protocol of the United Nations Framework Convention on Climate Change, industrialized nations can use certain forest biomass sinks to meet their greenhouse gas emissions reduction commitments. Therefore, we analyzed 19 years of data from remote-sensing spacecraft and forest inventories to identify the size and location of such sinks. The results, which cover the years 1981-1999, reveal a picture of biomass carbon gains in Eurasian boreal and North American temperate forests and losses in some Canadian boreal forests. For the 1.42 billion hectares of Northern forests, roughly above the 30th parallel, we estimate the biomass sink to be 0.68 ± 0.34 billion tons carbon per year, of which nearly 70% is in Eurasia, in proportion to its forest area and in disproportion to its biomass carbon pool. The relatively high spatial resolution of these estimates permits direct validation with ground data and contributes to a monitoring program of forest biomass sinks under the Kyoto protocol.
The isolation of a patient's metastatic cancer cells is the first, enabling step toward treatment of that patient using modern personalized medicine techniques. Whereas traditional standard-of-care ...approaches select treatments for cancer patients based on the histological classification of cancerous tissue at the time of diagnosis, personalized medicine techniques leverage molecular and functional analysis of a patient's own cancer cells to select treatments with the highest likelihood of being effective. Unfortunately, the pure populations of cancer cells required for these analyses can be difficult to acquire, given that metastatic cancer cells typically reside in fluid containing many different cell populations. Detection and analyses of cancer cells therefore require separation from these contaminating cells. Conventional cell sorting approaches such as Fluorescence Activated Cell Sorting or Magnetic Activated Cell Sorting rely on the presence of distinct surface markers on cells of interest which may not be known nor exist for cancer applications. In this work, we present a microfluidic platform capable of label-free enrichment of tumor cells from the ascites fluid of ovarian cancer patients. This approach sorts cells based on differences in biomechanical properties, and therefore does not require any labeling or other pre-sort interference with the cells. The method is also useful in the cases when specific surface markers do not exist for cells of interest. In model ovarian cancer cell lines, the method was used to separate invasive subtypes from less invasive subtypes with an enrichment of ~ sixfold. In ascites specimens from ovarian cancer patients, we found the enrichment protocol resulted in an improved purity of P53 mutant cells indicative of the presence of ovarian cancer cells. We believe that this technology could enable the application of personalized medicine based on analysis of liquid biopsy patient specimens, such as ascites from ovarian cancer patients, for quick evaluation of metastatic disease progression and determination of patient-specific treatment.
We derive the stellar mass fraction in the galaxy cluster RXC J2248.7-4431 observed with the Dark Energy Survey (DES) during the Science Verification period. We compare the stellar mass results from ...DES (five filters) with those from the Hubble Space Telescope Cluster Lensing And Supernova Survey (CLASH; 17 filters). When the cluster spectroscopic redshift is assumed, we show that stellar masses from DES can be estimated within 25 per cent of CLASH values. We compute the stellar mass contribution coming from red and blue galaxies, and study the relation between stellar mass and the underlying dark matter using weak lensing studies with DES and CLASH. An analysis of the radial profiles of the DES total and stellar mass yields a stellar-to-total fraction of f... = (6.8 plus or minus 1.7) x 10 super( -3) within a radius of r sub( 200c) ... 2 Mpc. Our analysis also includes a comparison of photometric redshifts and star/galaxy separation efficiency for both data sets. We conclude that space-based small field imaging can be used to calibrate the galaxy properties in DES for the much wider field of view. The technique developed to derive the stellar mass fraction in galaxy clusters can be applied to the ~100 000 clusters that will be observed within this survey and yield important information about galaxy evolution. (ProQuest: ... denotes formulae/symbols omitted.)
Sleep apnea is a common disorder that represents a global public health burden. KCNK3 encodes TASK-1, a K
channel implicated in the control of breathing, but its link with sleep apnea remains poorly ...understood. Here we describe a new developmental disorder with associated sleep apnea (developmental delay with sleep apnea, or DDSA) caused by rare de novo gain-of-function mutations in KCNK3. The mutations cluster around the 'X-gate', a gating motif that controls channel opening, and produce overactive channels that no longer respond to inhibition by G-protein-coupled receptor pathways. However, despite their defective X-gating, these mutant channels can still be inhibited by a range of known TASK channel inhibitors. These results not only highlight an important new role for TASK-1 K
channels and their link with sleep apnea but also identify possible therapeutic strategies.
We present an early-phase g-band light curve and visual-wavelength spectra of the normal Type Ia supernova (SN) 2013gy. The light curve is constructed by determining the appropriate S-corrections to ...transform KAIT natural-system B- and V-band photometry and Carnegie Supernova Project natural-system g-band photometry to the Pan-STARRS1 g-band natural photometric system. A Markov chain Monte Carlo calculation provides a best-fit single power-law function to the first ten epochs of photometry described by an exponent of 2.16+0.06−0.06 2 . 16 − 0.06 + 0.06 $ 2.16^{+0.06}_{-0.06} $ and a time of first light of MJD 56629.4+0.1−0.1 56629 . 4 − 0.1 + 0.1 $ 56629.4^{+0.1}_{-0.1} $ , which is 1.93+0.12−0.13 1 . 93 − 0.13 + 0.12 $ 1.93^{+0.12}_{-0.13} $ days (i.e., < 48 h) before the discovery date (2013 December 4.84 UT) and −19.10+0.12−0.13 − 19 . 10 − 0.13 + 0.12 $ -19.10^{+0.12}_{-0.13} $ days before the time of B-band maximum (MJD 56648.5 ± 0.1). The estimate of the time of first light is consistent with the explosion time inferred from the evolution of the Si IIλ6355 Doppler velocity. Furthermore, discovery photometry and previous nondetection limits enable us to constrain the companion radius down to Rc ≤ 4 R⊙. In addition to our early-time constraints, we used a deep +235 day nebular-phase spectrum from Magellan/IMACS to place a stripped H-mass limit of < 0.018 M⊙. Combined, these limits effectively rule out H-rich nondegenerate companions.
Identifying dairy cattle experiencing heat stress and adopting appropriate mitigation strategies can improve welfare and profitability. However, little is known about how cattle use heat abatement ...resources (shade, sprayed water) on drylot dairies. It is also unclear how often we need to observe animals to measure high heat load, or the relevance of specific aspects of this response, particularly in terms of panting. Our objectives were to describe and determine sampling intervals to measure cattle use of heat abatement resources, respiration rate (RR) and panting characteristics (drooling, open mouth, protruding tongue), and to evaluate the relationship between the latter 2. High-producing cows were chosen from 4 drylots (8 cows/dairy, n=32) and observed for at least 5.9h (1000 to 1800h, excluding milking) when air temperature, humidity, and the combined index averaged 33°C, 30%, and 79, respectively. Use of heat abatement resources was recorded continuously; RR and the presence and absence of each panting characteristic were recorded every 5min. From the observed values, estimates using the specified sub-sampling intervals were calculated for heat abatement resource use (1, 5, 10, 15, 20, 30, 60, 90, and 120min), and for RR and panting (10, 15, 20, 30, 60, 90, and 120min). Estimates and observed values were compared using linear regression. Sampling intervals were considered accurate if they met 3 criteria: R2≥0.9, intercept=0, and slope=1. The relationship between RR and each panting characteristic was analyzed using mixed models. Cows used shade (at corral or over feed bunk) and feed bunk area (where water was sprayed) for about 90 and 50% of the observed time, respectively, and used areas with no cooling for 2min at a time, on average. Cows exhibited drooling (34±4% of observations) more often than open mouth and protruding tongue (11±3 and 8±3% of observations, respectively). Respiration rate varied depending on the presence of panting (with vs. without drool present: 97±3 vs. 74±3 breaths/min; open vs. closed mouth: 104±4 vs. 85±4 breaths/min; protruding vs. non-protruding tongue: 105±5 vs. 91±5 breaths/min). Accurate estimates were obtained when using sampling intervals ≤90min for RR, ≤60min for corral shade and sprayed water use, and ≤30min for drooling. In a hot and dry climate, cows kept in drylots had higher RR when showing panting characteristics than when these were absent, and used shade extensively, avoiding areas with no cooling. In general, 30min intervals were most efficient for measuring heat load responses.
Alcohol consumption is an established risk factor for colorectal cancer (CRC). However, while studies have consistently reported elevated risk of CRC among heavy drinkers, associations at moderate ...levels of alcohol consumption are less clear. We conducted a combined analysis of 16 studies of CRC to examine the shape of the alcohol–CRC association, investigate potential effect modifiers of the association, and examine differential effects of alcohol consumption by cancer anatomic site and stage. We collected information on alcohol consumption for 14,276 CRC cases and 15,802 controls from 5 case‐control and 11 nested case‐control studies of CRC. We compared adjusted logistic regression models with linear and restricted cubic splines to select a model that best fit the association between alcohol consumption and CRC. Study‐specific results were pooled using fixed‐effects meta‐analysis. Compared to non‐/occasional drinking (≤1 g/day), light/moderate drinking (up to 2 drinks/day) was associated with a decreased risk of CRC (odds ratio OR: 0.92, 95% confidence interval CI: 0.88–0.98, p = 0.005), heavy drinking (2–3 drinks/day) was not significantly associated with CRC risk (OR: 1.11, 95% CI: 0.99–1.24, p = 0.08) and very heavy drinking (more than 3 drinks/day) was associated with a significant increased risk (OR: 1.25, 95% CI: 1.11–1.40, p < 0.001). We observed no evidence of interactions with lifestyle risk factors or of differences by cancer site or stage. These results provide further evidence that there is a J‐shaped association between alcohol consumption and CRC risk. This overall pattern was not significantly modified by other CRC risk factors and there was no effect heterogeneity by tumor site or stage.
What's new?
Heavy drinking is associated with increased colorectal cancer (CRC) risk, but there's debate about the impact of moderate drinking. Here, the authors conducted a combined analysis of 16 studies comprising 14,276 cases and 15,802 controls. By their analysis, drinking 1‐2 alcoholic beverages per day was associated with a reduced risk of CRC, compared with rare or no alcohol consumption. With 3 or more drinks per day, CRC risk rises. The authors suggest that moderate alcohol consumption may reduce inflammation and DNA damage, although they acknowledge that more research is needed to understand the biochemical mechanism at work.
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